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Citations for Thermo Fisher :
8351 - 8400 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... qRT-PCRs reactions were performed in technical duplicate using a StepOnePlus real-time PCR system (Thermo Fisher Scientific). Gene expression was calculated using ΔCT relative to the gyrA housekeeping gene and comparative ΔΔCT was determined by comparison of each time point to the ΔCT of the initial 2 h sample (∼0.05 OD600).
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplified DNA of the target site of interest was purified via a GeneJet PCR Purification kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and used for quantitative real-time PCR analysis (StepOne-PlusTM Real-Time PCR System, Applied Biosystems, Darmstadt, Germany). Relative expression levels were calculated using the ΔΔCt method ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, USA).
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bioRxiv - Immunology 2023Quote: ... mRNA levels were evaluated in a two-step PCR reaction (StepOnePlus Real-Time PCR Cycler, Applied Biosystems, USA) with 60°C annealing/extension temperature for 40 cycles using the 2x qPCRBIO SyGreen Mix Hi-ROX (PCR Biosystems Ltd. ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplicons from the second round PCR were then column purified using the PureLink Quick PCR Purification Kit (Invitrogen). Purified PCR products were sequenced using HiSeq 2500 (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... The qRT–PCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System (Thermo Fisher Scientific). The sequences of primers and probes for Adar1 p150 and GAPDH have been previously reported (16) ...
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bioRxiv - Genetics 2023Quote: ... 5 μl of PCR products were purified by ExoSAP-IT™ PCR Product Cleanup Reagent (Thermo Fisher Scientific) following manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed in a QuantStudio 12K Flex Real Time PCR System (Thermofisher Scientific, Waltham, MA) using TaqMan™ Gene Expression Master Mix (Applied Biosystems/Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR-were performed in technical triplicate using the TaqMan Fast Universal PCR Master Mix (Thermo Fisher #4352042) according to manufacturer’s instructions in either a ViiA7 or QuantStudio7 Flex Real-Time PCR machine ...
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bioRxiv - Immunology 2023Quote: ... mRNA levels were evaluated in a two-step PCR reaction (StepOnePlus Real-Time PCR Cycler, Applied Biosystems, USA) with 60°C annealing/extension temperature for 40 cycles using the 2x qPCRBIO SyGreen Mix Hi-ROX (PCR Biosystems Ltd. ...
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bioRxiv - Microbiology 2023Quote: ... Real time PCR assay was performed on Quant studio 5 real time PCR machine (Thermo Fisher Scientific, USA). Temperature profile employed is provided in Table S4.
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bioRxiv - Plant Biology 2023Quote: ... PCR products and DNA fragments containing DNA were cleaned up with the GeneJET PCR Purification Kit (Thermo Scientific) or Nucleospin gel and PCR cleanup kit.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was cloned in a TOPO TA vector (Thermo Fisher Scientific, pCR™ II-TOPO®) and 20 clones with Satb1 inserts were analyzed upon restriction enzyme digestion and Sanger sequencing indicating the presence of Satb1 cDNAs with different length ...
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bioRxiv - Neuroscience 2023Quote: ... and qRT-PCR was validated with the QuantStudio 1 Real Time PCR system (Applied Biosystems, Thermo Fisher Scientific). Expression level of target genes was calculated using the comparative method of relative quantification (2−ddCt ...
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bioRxiv - Neuroscience 2023Quote: ... and qRT-PCR was validated with the QuantStudio 1 Real Time PCR system (Applied Biosystems, Thermo Fisher Scientific). Expression level of target genes was calculated using the comparative method of relative quantification (2−ddCt ...
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bioRxiv - Immunology 2023Quote: ... The qRT-PCR experiment was done with an ABI 7500 Real Time PCR System (Applied Biosystems, Carlsbad, CA). Each cDNA sample of each target gene (TGF-β ...
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bioRxiv - Immunology 2023Quote: ... Real-time quantitative PCR was performed using the QuantStudio™ 3 Flex Real-Time PCR System (Applied Biosystems) and TaqMan™ Fast Advanced Master Mix ...
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bioRxiv - Bioengineering 2023Quote: ... quantitative real time PCR (qRT-PCR) was conducted using TaqMan™ Fast Advanced Master Mix (Thermo Fisher 444455) and analyzed on Quantstudio 7 Flex Real-Time PCR system (Thermo Fisher 4485701) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR progress was monitored by adding SYBR Green dye using StepOnePlus™ Real-Time PCR System (Applied Biosystems), and data were processed and quantified by StepOne™ Software (v2.0.2) ...
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bioRxiv - Bioengineering 2022Quote: Quantitative real time PCR (qPCR) was performed with a StepOnePlus™ Real-Time PCR System Upgrade (Life Technologies). All PCRs were performed with a final volume of 20 µL ...
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bioRxiv - Cancer Biology 2023Quote: ... real-time PCR (qPCR) was carried out in triplicates using Power SYBR Green PCR Master Mix (Applied Biosystems). Gene-specific ...
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bioRxiv - Cancer Biology 2022Quote: ... Nested PCR products were gel purified and cloned into pCR®2.1-TOPO® vectors (Invitrogen, Carlsbad, CA). Plasmid DNA from 10 positive clones was sequenced using an ABI 3730 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplicons from the second round PCR were then column purified using the PureLink Quick PCR Purification Kit (Invitrogen). Purified PCR products were sequenced using HiSeq 2500 (Illumina) ...
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bioRxiv - Immunology 2023Quote: ... The mRNA levels were measured by quantitative PCR using a Step-One Real-Time PCR system (Applied Biosystems) with THUNDERBIRD probe qPCR Mix (TOYOBO ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were treated with ExoSAP-IT™ Express PCR Product Cleanup reagent (Thermo Fisher Scientific Baltics, Lithuania), according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: Real-time PCR was performed using Applied Biosystems HT7900 with Power SYBR Green PCR Master Mix (Life Technologies) following standard protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplification was conducted in a GeneAmp PCR System 2700 thermal cycler (Applied Biosystems, Foster City, California, USA). The PCR products were purified by using a High Pure PCR product purification kit (B A High Pure PCR Product Purification Kit (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed in biological triplicate and technical quadruplicate using Power SYBR PCR master mix (Thermo Fisher) for Dkk4 and En1 ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was used as template for quantitative PCR (aPCR) using Power SYBR Green PCR master mix (ThermoFisher). Transcripts were normalized to Gapdh expression and relative expression shown as 2-ΔΔCt compared to the average ΔCt of experiment-matched controls ...
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bioRxiv - Molecular Biology 2022Quote: The qRT-PCR assays were performed using the QuantStudio™ 5 Real-Time PCR System (ThermoFisher Scientific, USA) with the following cycling conditions for CHIKV ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed on a StepOne Plus Real-Time PCR System (Applied Biosystems, Foster, CA, USA) using SFCgreen® (BIOFACT ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were then purified using the ExoSAP-IT Express PCR cleanup kit (Applied Biosystems, Foster City, CA) and sequenced at the Genomics Research Core at the University of Pittsburgh ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was performed using a 7500 Real-Time PCR system and SYBR Green reagents (Applied Biosystems). Specific primer sets used were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were TOPO cloned using Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing (ThermoFisher K287520), transformed into 5-alpha Competent E ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR product was cloned into pJet1.2/blunt using CloneJET PCR Cloning Kit (K1231, Thermo Fisher Scientific, USA). The correct clone was selected by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was performed using a 7500 Real-Time PCR system and SYBR Green reagents (Applied Biosystems).
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bioRxiv - Microbiology 2023Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (Quant Studio 6 Flex; Applied Biosystems) with SYBR green PCR master mix (Life Technologies) ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was performed using a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA). The PCR cycling conditions were as follows ...
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bioRxiv - Immunology 2023Quote: ... The samples were subjected to 45 PCR cycles in a thermocycler StepOnePlus Real-Time PCR System (Applied Biosystems). Abundance of satellite DNA from T ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragments of interest were generated by PCR with further purification using the PureLink™ PCR Purification Kit (Invitrogen), and then automated Sanger sequencing was performed at IBTEC (UNESP – Botucatu) ...
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bioRxiv - Cancer Biology 2023Quote: ... Using qRT-PCR (QuantStudio 6, ThemoFisher Scientific) and the SYBR Green Real-time PCR master mix (Thermo Scientific), the expression levels of certain genes were determined.
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bioRxiv - Microbiology 2023Quote: ... The generated PCR products were assembled and cloned into the pCR™8/GW/TOPO™ vector (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Quantitative PCR was performed in triplicate on a QuantStudio 12K Flex Real-Time PCR System (Thermo Fisher Scientific) for five homozygous carriers of the NOA1 frameshift insertion and five matched controls ...
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bioRxiv - Bioengineering 2024Quote: ... Transcript abundance was determined by quantitative PCR using SYBR Green PCR mix (Applied Biosystems, Thermo Fisher Scientific #A25778). The primers were designed according to specific targets (Table 1) ...
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bioRxiv - Bioengineering 2024Quote: ... Transcript abundance was determined by quantitative PCR using SYBR Green PCR mix (Applied Biosystems, Thermo Fisher Scientific #A25778). The primers were designed according to specific targets (Table 1) ...
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bioRxiv - Immunology 2024Quote: ... Viia 7 Real-Time PCR system with a Power SYBR green PCR master mix kit (Applied Biosystems, 4367660) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... Thermal cycling of PCR was run using Phusion High-Fidelity PCR Master Mix with HF Buffer (ThermoFisher, USA) for 30 cycles ...
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bioRxiv - Microbiology 2024Quote: ... Real time PCR assay was performed on Quant studio 5 real time PCR machine (Thermo Fisher Scientific, USA). Temperature profile employed is provided in Table S3.
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bioRxiv - Genetics 2024Quote: All PCR reactions were performed using the Phusion High-Fidelity PCR Master Mix (Thermo Scientific, Lafayette, CO, USA) or KAPA HiFi DNA polymerase (Roche) ...