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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: zebrafish roraa 3′UTR was amplified from zebrafish genomic DNA with SuperScript III RT-kit (Invitrogen #18080044) and cloned into psiCHECK2 (Promega ...
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bioRxiv - Microbiology 2020Quote: MeltMan [57] SVIP-MPv2 RT-qPCR reactions were performed by adding the SYTO82 dye (Thermo Fisher Scientific) in a final concentration of 0.8μM ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed with PBS, BSA (1%), incubated (5min, RT) with a viability dye (Aqua-dye, Invitrogen) and surface stained with titrated amounts of antibodies against i ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed using Taqman RNA-to-CT 1-step kit (Thermo fisher scientific, Waltham, MA) in a total volume of 10 µl and a template concentration of 50 ng/µl according to manufacturer’s recommendations ...
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bioRxiv - Systems Biology 2022Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Immunology 2022Quote: ... and was reverse-transcribed with a RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific, Catalog number: K1691). Real-time PCR was performed with Fast SYBR Green Master Mix and a 7500 Fast Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was carried out using Random Hexamers (Integrated DNA Technologies) and Superscript III RT enzyme (Invitrogen). Primers specific to CAP206-CH12 heavy and light chains (IGHV1-69 and IGKV3-20 ...
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bioRxiv - Synthetic Biology 2022Quote: ... samples were incubated for 3 hours at RT with alexa-conjugated secondary antibodies (Invitrogen or Jackson ImmunoResearch). Samples were mounted onto glass slides using Fluoromount G (Southern Biotech).
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bioRxiv - Microbiology 2022Quote: A variant-specific RT-qPCR with the TaqMan™ SARS-CoV-2 kit Mutation Panel (Applied Biosystems) was used to screen the VOC/VOI mutations S_L452R and S_T478K following manufacturer ‘s protocol.
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bioRxiv - Cell Biology 2022Quote: ... Fixed HUVEC were blocked for 1h at RT in blocking solution (5% FBS, 2X antibiotic-antimycotic (Gibco), 0.1% sodium azide (s2002-100G ...
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bioRxiv - Genomics 2019Quote: ... cDNA was synthetized by reverse transcription of 500 ng of total RNA using M-MLV RT (Invitrogen) and random hexamer priming according to the manufacturer’s protocol and including RNase OUT (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were fixed for 12 min at RT by adding methanol-free paraformaldehyde (PFA, Thermo Fisher Scientific) to a final concentration of 4% ...
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bioRxiv - Cancer Biology 2019Quote: ... 40 U RNase out RNase inhibitor and 200 U SuperScript™ II RT (all from Invitrogen™) to each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... 4sU-RNA and total RNA samples were retrotranscribed using RevertAid RT Reverse Transcription kit (Thermo Fisher #K1691), treated with Dnase I (Thermo Fisher #18068015 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were incubated with HRP-secondary antibodies for 1 hour at RT and revealed using ECL (Invitrogen) and Chemidoc™ MP Imaging System (Biorad) ...
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bioRxiv - Cell Biology 2019Quote: ... Blocking was performed for 30 min at RT and incubation with rabbit anti-human AlexaFluor 647 (Invitrogen) that binds to the Tmab Fc region of T-DM1 and Accum-T-DM1 ...
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bioRxiv - Genetics 2020Quote: ... cDNA was reverse transcribed from 1 ìg samples of total RNA using Superscript IV Vilo RT(Invitrogen). Diluted cDNA was used as a template in PCR reactions carried out in Power SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... treatment and cDNA synthesis was performed with 1 μg total RNA using SuperScriptIII RT (Thermo Fisher Scientific). Gene expression was measured by qPCR on a qPCR cycler (CFX96 ...
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bioRxiv - Molecular Biology 2019Quote: ... All steps were performed at room temperature (RT) using an autostainer 480S and protocol by Thermo Scientific. In brief ...
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bioRxiv - Cell Biology 2020Quote: ... The gene expression levels were calculated with Rt (2−ΔΔCT) values by StepOne Software v2.1 (Applied Biosystems). Primer sequences used in qRT◻ PCR were listed in TABLE 2.
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT using Pierce™ Clear Milk Blocking Buffer (ThermoFisher Scientific). Blots were incubated overnight at 4°C with mouse anti-FLAG tag mAB (Cell Signaling Technology ...
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bioRxiv - Genetics 2020Quote: ... The ligated mRNA was reverse-transcribed into cDNA using the SuperScript™ III RT kit (Invitrogen, USA). All the primers used to reverse-transcribe and to amplify the junctional 5’-fragment of the cDNA are listed in Table S2 ...
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bioRxiv - Immunology 2020Quote: ... Five μg of cDNA were used for RT-qPCR using Taqman Universal Master Mix II (Applied Biosystems). Primers and probes are listed in Table S1 ...
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bioRxiv - Neuroscience 2019Quote: ... RT-qPCR was run with obtained cDNA using TaqMan Fast Advance Master Mix (Life Technologies, cat # 4444963), and TaqMan® Array Card 24 (Life technology ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by gel size selection of 5′ adaptor ligated RNAs and reverse transcription (SuperScript III RT, Invitrogen) with 3′ random priming ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated for 1 hour at RT with secondary antibodies (1:500, goat Alexa Fluor®, Invitrogen) in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: ... One μg of RNA was used for RT (Superscript® III Reverse Transcriptase, 18080044, Invitrogen, Carlsbad, CA). qPCR was performed using SyBR Green (1725122 ...
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bioRxiv - Neuroscience 2019Quote: ... Wako #019-19741) followed by secondary antibody solution (4 h, RT, Alexa-Fluor 488, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Cell Biology 2019Quote: ... RT was completed with the high-capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA), following manufacturer instructions ...
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bioRxiv - Cell Biology 2019Quote: ... sections were incubated with BODIPY 493/503 for 20min at RT (Molecular Probes Inc., Eugene, OR, USA). Mounting medium contained DAPI (4’ ...
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bioRxiv - Genetics 2020Quote: ... 20 U SUPERase•In™ RNase Inhibitor and 200 U SuperScript II RT (ThermoFisher Scientific, cat. 18064014). Reactions were incubated at 42°C for 2 h and then RT was heat-inactivated at 75°C for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... The one-step RT-qPCR was run using a qPCR system (QuantStudio 3, Thermo Fisher Scientific, USA) with the following thermocycle ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time RT-qPCR was carried out with the PowerUp SYBR Green Mastermix (ThermoFisher Scientific, Vilnius, Lithuania) at the StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... washed for 2 min in TBST at RT and mounted with ProLong Diamond Antifade Mountant (P36961 Invitrogen). Images were acquired on a Zeiss Axio Scan.Z1 using a ×20 objective and ZEN 2 software (Zeiss ...
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bioRxiv - Genetics 2021Quote: ... For RT-QPCR 20 mg of RNA was treated with 1 μL of TURBO DNase I (Ambion) for 1 hr at 37°C ...
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bioRxiv - Genomics 2021Quote: ... treatment and cDNA synthesis was performed with 1 μg total RNA using SuperScriptIII RT (Thermo Fisher Scientific). Gene expression was measured by qPCR on a Quantstudio5 cycler (ThermoFischer Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... for 45 minutes at RT with gentle agitation and further digestion halted by addition of SUPERase:In (Ambion). Illustra Microspin Columns S-400 HR (GE healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 3.5 hours at RT with Alexa-conjugated secondary antibodies (Invitrogen or Jackson ImmunoResearch). Sections were mounted onto glass microscope slides ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... preamplified cDNA was diluted 40 times and RT-qPCR was performed using SYBR Green Master Mix (ThermoFisher) and the forward (FW ...
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bioRxiv - Immunology 2021Quote: ... beads were then suspended in 10 μL of RT mix [1× First Strand buffer (Thermo Fisher Scientific), 1 mM dNTP ...
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bioRxiv - Genetics 2019Quote: ... GEM-reverse transcriptions (GEM-RTs) were performed in a Veriti 96-well thermal cycler (Thermo Fisher Scientific). After reverse transcription ...
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bioRxiv - Biochemistry 2020Quote: RT-QuIC reactions were performed in black 96-well plates with a clear bottom (Nalgene Nunc International). We preloaded plates with 6 glass or silica beads (1 mm in diameter ...
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bioRxiv - Immunology 2021Quote: ... The RT-qPCR reaction used TaqPath 1-Step Multiplex Master Mix (catalog number A28527; Thermo Fisher Scientific) along with the 2019-nCoV RUO Kit (catalog number 10006713 ...
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bioRxiv - Bioengineering 2022Quote: ... for 20 minutes at RT and stained with AlexaFluor-488 conjugated phalloidin (Invitrogen A12379, diluted 1:200) and DAPI (Fisher 62248 ...
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bioRxiv - Genomics 2022Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Microbiology 2022Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) using random hexamers ...
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bioRxiv - Plant Biology 2022Quote: ... and cDNA was synthesized using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific). Primers were designed using QuantPrime 94 ...
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bioRxiv - Molecular Biology 2023Quote: Reverse transcription (RT) into complementary DNA (cDNA) was conducted using Maxima Reverse Transcriptase (EP0752, Thermo Fisher Scientific) and Oligo(dT)18 primer (SO131 ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) was performed using 0.5-1 μg total RNA as template and Superscript II (Invitrogen) according to the instructions with the following modifications ...
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bioRxiv - Microbiology 2022Quote: ... for 1h at RT following 30 min of incubation with secondary antibody (1:200, A11034, Life Technologies) and nuclei were visualized by using DAPI (Sigma Aldrich).