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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... cDNA were generated using SuperScript® VILOTM cDNA Synthesis Kit (ThermoFisher Scientific).Real-time PCR reactions were carried out using Power SYBR® Green PCR Master Mix (ThermoFisher Scientific) and run on StepOne Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative PCR were performed with an Applied Biosystems 7500 real-time PCR detection system (ThermoFisher Scietific, USA) and Applied Biosystems 7500 software version 2.0.6 was used for data analysis.
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR was performed on the Step One Plus Real-Time PCR System (Applied Biosystems) using iTaq SYBR Green Supermix with ROX (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... All PCRs were performed using the Platinum II Hot-Start PCR Master Mix (Invitrogen, ref. 14000-013), containing 8 µL of the cDNA template ...
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bioRxiv - Neuroscience 2023Quote: ... Polymerase chain reaction (PCR) was conducted with the Taqman® Fast Universal PCR Master Mix (Applied Biosystems). The TaqMan® probes and primers that were used are as follows ...
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bioRxiv - Microbiology 2023Quote: ... The final PCR products were purified by PureLink PCR purification kit (Thermo Fisher Scientific, catalog no. K310002) and submitted for next-generation sequencing using Illumina MiSeq PE250.
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bioRxiv - Immunology 2023Quote: Quantitative PCRs (qPCR) were run and analyzed on a QuantStudio3 Real-Time PCR System (Thermo Fisher Scientific) according to the PowerUP SYBR Green (Thermo Fisher Scientific #25741 ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were cloned into a blunt vector using the Zero Blunt PCR cloning kit (#K270040, ThermoFisher) to sequence the two alleles independently.
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bioRxiv - Cell Biology 2023Quote: ... The PCR product was cloned into the pJET1.2 plasmid using the CloneJET PCR Cloning Kit (Thermo Scientific), according to the manufactureŕs instructions ...
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bioRxiv - Neuroscience 2023Quote: ... All qRT-PCR reactions were performed in triplicate on a StepOnePlusTM Real- Time PCR System (Applied Biosystems) and relative expression calculated using the 2-ΔΔCT method with data normalized to GAPDH or SNORD48 (see Table XX for primer sequences).
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified using the PureLink™ Quick Gel Extraction and PCR Purification Combo Kit (Invitrogen). The purified PCR products were Sanger sequenced at a core facility (IBTEC) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and colony PCR was performed using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific). Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR was performed in the QuantStudioTM 3 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA) using the PowerUpTM SYBRTM Green Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR amplified fragment was subcloned into pCR-Blunt II-TOPO (Zero blunt TOPO cloning kit, Invitrogen) for sequence validation by Sanger sequencing (Eurofins) ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time PCR was performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems) after mixing cDNA ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed by 7900HT FAST Real-time PCR system (Applied Biosystems, Foster City, CA) with a SYBR green I mix ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real time PCR (qRT-PCR) was done using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) along with custom designed ...
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bioRxiv - Genetics 2024Quote: ... The PCR fragments that were shorter than that of the wildtype were subcloned into pCR-TOPO (Invitrogen) and sequenced using the M13 forward and reverse primers.
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Candida albicans enhance Staphylococcus aureus virulence by progressive generation of new phenotypesbioRxiv - Microbiology 2024Quote: Quantitative real-time PCR (qRT-PCR) was performed with the PowerUp SYBR Green Master Mix (Applied Biosystems) in a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... and used as a template for PCR amplifications with Platinum Green Hot Start PCR Master Mix (Invitrogen) and primers in Supplementary Table 7 ...
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bioRxiv - Cell Biology 2024Quote: ... which were subsequently used for PCR screening using Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific). PCR positive clones were then expanded and tested for their response to pomalidomide treatment (positive control compound ...
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bioRxiv - Synthetic Biology 2024Quote: ... Real-time PCR (qPCR) was performed using the Power SYBR® Green PCR Master Mix (ThermoFisher Scientific) in a LightCycler 480 instrument (Roche ...
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bioRxiv - Cell Biology 2020Quote: Each TMT labeled peptide set was fractionated to improve depth of proteome coverage using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific, Part 84868). The fractionation was performed according to the manufacturer’s protocol and yielded 8 peptide fractions for analysis ...
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bioRxiv - Cell Biology 2020Quote: ... a first series of PCR was performed using gene-specific barcodes placed at the extremities of each oligo to amplify specific probe sets using a hot start Phusion DNA Polymerase (Thermo Fisher Scientific, F549L). A second series of PCR was done to add the T7 RNA polymerase promoter using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated as described by the manufacturer with an on-column DNA digestion using the PureLink™ DNase Set (Invitrogen, Thermo Fisher Scientific). RNA was eluted in nuclease-free H2O and stored at −20° C for short-term storage or −80° C for long-term storage.
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bioRxiv - Immunology 2021Quote: ... then permeabilized and stained with antibodies for intracellular protein for 1 h using the Foxp3 Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA). Data were collected in a FACSCalibur flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... SHIV RNA was visualized with the 1-Plex ViewRNA™ ISH Tissue Assay Kit using SIVmac239 or Beta actin (positive control) probe sets that were dectected via the ViewRNA™ Chromogenic Signal Amplification Kit (all from ThermoFisher, Waltham, MA). These two sequential slides were indivudually imaged with a Zeiss AxioObserver microscope and AxioCam MRm camera ...
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bioRxiv - Microbiology 2020Quote: ... followed by hybridization with Affymetrix GeneChip Mouse Gene 1.0 ST Array comprising more than 750,000 unique 25-mer oligonucleotide that contains over 28000 gene-level probe sets (Affymetrix, Santa Clara, CA, USA). Arrays were washed and stained using GeneChip Fluidic Station 450 as per the protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the Shambhala algorithm [20] was performed in R package HARMONY (https://github.com/oncobox-admin/harmony) for a set of 6546 genes in common between the two platforms (Affymetrix microarrays and RNA sequencing). For the exploration of relationships between the samples cluster dendrograms were built using R package ComplexHeatmap [21] and the statistical significance of hierarchical clustering was assessed with bootstrapping method using the R package pvclust [22] ...
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bioRxiv - Biophysics 2022Quote: ... A 16-hour dialysis was set-up by placing the reconstitution mixture in a 7 kDa MWCO Slide-A-Lyzer MINI Dialysis Device (Thermo Scientific cat # 69560) and placed in a flotation device in high-salt buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... were aliquoted into a final volume of 100 µL of 100 mM TEAB and labeled with tandem-mass tag (TMT) isobaric labels (TMT10 or 11plex™ Isobaric Label Reagent Set, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... IL-5 and IFN-γ were quantified using Invitrogen™ eBioscience™ ELISA Ready-SET-Go!™ (Thermo Fisher Scientific, Waltham, MA, USA). IP-10 (CXCL10) ...
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bioRxiv - Cell Biology 2019Quote: ... All 96 blood samples (4 sets from each donor) were collected into one 96-tube rack and 200 μL of PB-MAX™ karyotyping media (Thermo Fisher Scientific) was added to each 20 μL blood sample ...
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bioRxiv - Neuroscience 2019Quote: ... using a 2X/0.06 NA air objective on an EVOS FL Cell Imaging System equipped with DAPI filter set (λex: 357/44 nm, λem: 447/60 nm; ThermoFisher Scientific, Waltham, MA). Images were collected at 0.1 fps and manually traced to measure slice perimeter and surface area using IMOD (https://bio3d.colorado.edu/imod/) ...
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bioRxiv - Cell Biology 2020Quote: ... the same maximal quantity of peptides was used for TMT labelling using a TMT10plex™ Isobaric Label Reagent Set (#90110, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... for 30 minutes at room temperature and then fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher, 00-5523-00) following kit guidelines ...
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bioRxiv - Cell Biology 2020Quote: siRNA sequences were Dharmacon SMART siGenome on-target plus set of 4 duplexes and scrambled siRNA negative control from Thermo Scientific (Lafayette, CO). Tpp1 targeting siRNAs were (1-GGGCUGAGUUUCAUCACUAUU ...
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bioRxiv - Microbiology 2020Quote: TaqMan RT-PCR was performed with the N2 primer/probe set and BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific) [31] using the QuantStudio 5 system (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Immunology 2021Quote: ... washed twice with FACS buffer and then fixed/permeabilized for 40 mins at 4°C in the dark using the eBioscience™ Intracellular Fixation & Permeabilization Buffer Set (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface markers then fixed and permeabilized for at least 16 h using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific). Cells were then stained with directly labeled Abs to NOS2 (CXNFT ...
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bioRxiv - Immunology 2021Quote: ... Amplicons were generated using 100 ng DNA as input in a final volume of 40 μL (consisting of 8 μl 5x Phusion GC buffer (Phusion High-Fidelity DNA Polymerase set (ThermoFisher Scientific, Cat#F534), 0.8 μL dNTPs (dNTP Set ...
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bioRxiv - Microbiology 2022Quote: ... RNA was isolated as described by the manufacturer with an on-column DNA digestion using the PureLink™ DNase Set (Invitrogen, Thermo Fisher Scientific). RNA was eluted in nuclease-free H2O and stored at -20° C for short-term storage or -80° C for long-term storage.
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bioRxiv - Immunology 2022Quote: ... cells were next permeabilized and fixed for 30min according to the eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific #00-5523-00). Fluorescent antibodies targeting the FoxP3 marker were diluted in the permeabilization buffer and incubated with the fixed cells for 1h ...
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bioRxiv - Cancer Biology 2022Quote: Expression levels of 754 miRNAs were quantified using the TaqMan Human MicroRNA Array A+B Card Set v3.0 (Applied Biosystems, Foster City, CA).
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bioRxiv - Plant Biology 2024Quote: ... The flow rate of the mobile phase was set at 0.5 mL / min and consisted of a 0.1% formic acid solution (A) (A117-50, Thermo Fisher Scientific, Waltham, MA, USA) and a 0.1% formic acid in acetonitrile (B ...
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bioRxiv - Pathology 2024Quote: ... Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific, Cat#90110) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were centrifuged (300rcf, 7min, 4°C) and fixed (1h, 4°C) using the eBioscience Foxp3/Transcription factor staining buffer set (ThermoFisher #00-5523-00) fixation/permeabilization reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... for the discovery set and the Recover AllTM Total Nucleic Acid Isolation Kit for the French external validation cohort (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Pellets were set to dry on the bench before being resuspended in 20 μl of The RNA Storage Solution (Ambion, Thermo Fisher Scientific) and stored at -80 °C.
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bioRxiv - Genomics 2024Quote: ... 100 ng of each probe set was then analyzed on a 12.5% Urea-PAGE gel stained with SYBR-Gold (Thermo Fisher Scientific, Waltham, MA).