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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 18μl of 1X SuperScript III Reverse Transcriptase reaction buffer (SSIII-RT; Thermo Fisher) containing 1μl of each 5′-fluorescein labeled primer (10 pmol/μl ...
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bioRxiv - Systems Biology 2024Quote: ... RT–qPCR was performed in triplicates using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, A25742) on QuantStudio 6/7 qPCR instruments ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples further washed 2X with PBS at RT and mounted with ProLong Gold Antifade Mountant (P36930, ThermoFisher).
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... cDNA synthesis was further carried out using RevertAid H minus reverse transcriptase (RT) enzyme (Thermo Fisher Scientific). The reaction mixture included RNA template of 4 µg ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription (RT) was performed using 1 μg of total RNA as template and Superscript II (Invitrogen) according to the instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Each target or multiplex combination was prepared with TaqPath 1-Step RT-qPCR Master Mix (ThermoFisher, A15300) as per the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with the Maxima First Strand cDNA Synthesis for RT-qPCR kit (Thermo Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1.5 hrs (RT) with donkey anti-rabbit Alexa 647 antibody (1:500; A32795; ThermoFisher Scientific) diluted in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... and was reverse-transcribed with a RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific, Catalog number: K1691).
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bioRxiv - Cancer Biology 2023Quote: The expression levels of ATE1 were analyzed by RT-qPCR on the ABI 7500 (Thermo Fisher Scientific). Primers were designed to amplify a region containing 80–120 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... which was diluted 1:10 for RT-qPCR measurements using PowerUp SYBR Green Master Mix (A25742, ThermoFisher. QuantStudio 5 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR reactions were prepared using the Fast SYBR Green Fast Mix (Applied Biosystems, catalog no. 4385610). Quantification was performed using the ΔΔCt method.
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bioRxiv - Cell Biology 2023Quote: ... Aortic root cryosections were dried at RT for 10 minutes and fixed in acetone (Acros organics, 423245000) at -20⁰C for 3 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated for 4 hours at RT with a goat anti-rat-AF647 secondary antibody (polyclonal, Invitrogen). After secondary antibody ...
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bioRxiv - Pathology 2023Quote: ... RNA concentration was determined with a nanodrop and adjusted to 1 ug for RT reaction (ThermoFisher 4368814). 8 ng of cDNA was used for the SYBR green reagent (BioRad 1725270 ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated in secondary antibody for 1 hour at RT: goat anti-rabbit Alexa Fluor 488 (ThermoFisher), goat anti-chicken Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... Thawed and dried smears were incubated with 1x clear milk block (Thermo Fisher Scientific, 30 min, RT), mouse IgG1 specific to the ISAV HE (clone 3H6F8 [52] ...
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bioRxiv - Microbiology 2023Quote: ... cells were stained for 20 min at RT with the Fixable Blue Dead Cell Stain Kit (Invitrogen) to determine viability ...
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Induction of PARP7 Creates a Vulnerability for Growth Inhibition by RBN2397 in Prostate Cancer CellsbioRxiv - Cancer Biology 2023Quote: ... fixed at room temperature (RT) for 20 min using the 10% neutral buffered formalin (Thermo Scientific 5701), washed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed in triplicates for each sample using PowerUp SYBR Green Master Mix (Applied Biosystems) with a CFX384 Real-Time System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR reactions were carried out using a SYBR green master mix (Applied Biosystems, Waltham, MA, USA) on a QuantStudio 3 qPCR system (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The RT reaction was performed according to the manufacturer protocol SuperScript™ IV Reverse Transcriptase (Invitrogen, 18090050). Successively ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed with the Maxima First Strand cDNA Synthesis for RT-qPCR kit (Thermo Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Cell Biology 2023Quote: ... and then reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Genomics 2022Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for 1 hour at RT with a goat antirat Alexa Fluor 594 antibody (A11007, Thermofisher Scientific) diluted 1:1000 in 1x PBS/0.3% Triton X-100/1% BSA ...
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bioRxiv - Biophysics 2023Quote: ... Cell cultures were pelleted by centrifugation at 3000 rpm in a Sorvall Legend RT centrifuge (Thermo Scientific) and washed three times in sterile water ...
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bioRxiv - Cell Biology 2024Quote: ... The SMART RT reaction was performed at 42°C for 90□min using commercial SuperScript II (Invitrogen) and a TSO ...
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bioRxiv - Plant Biology 2024Quote: ... and approximately 1 μg of RNA was reverse transcribed using SuperScriptTM II RT (Invitrogen, Carlsbad, CA, USA) for cDNA synthesis following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Gene expression was determined by performing RT-qPCR in duplicate with PowerUpTM SYBRTM green master mix (Thermofisher) using a 7500 Fast system (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... the domes were incubated for 20 min at RT in the dark with Hoechst 33342 (Invitrogen, #H3570). The domes were washed again 5 times with 200 μl of working buffer and prepared for imaging with 200 μl of ProLong Glass mounting medium (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... washed (4 x 300 µL PBST) and blocked for 1h at RT with 1% BSA (Fisher Scientific) in PBS solution ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using SYBR GreenER™ qPCR SuperMix for ABI PRISM™ Instrument (ThermoFisher # 11760100). The primers used are the following ...
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bioRxiv - Molecular Biology 2023Quote: ... reverse transcription was performed during 15 min at 50°C using SuperScript IV RT Enzyme (Invitrogen,18090050), followed by heat inactivation for 5 minutes at 70°C ...
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bioRxiv - Neuroscience 2023Quote: Sections were first acclimatized at room temperature (RT) and further rehydrated in PBS (Life Technologies, 7001-36) for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Microbiology 2023Quote: ... mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer’s instructions) and random hexamers ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was reverse transcribed to yield cDNA using SuperScript III RT and random hexamers (Life Technologies) per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RT-qPCR was carried out by using the PowerTrackTM SYBR Green Master Mix (Thermo Fisher Scientific, A46110). Primers were designed to amplify the junction between two exons using a web-based primer design program ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked for 1 h at RT with nucleic acid detection blocking buffer (Life Technologies), then probed for 1 h at RT with streptavidin-HRP 1:5,000 (Genescript ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Genomics 2023Quote: ... RT-qPCR samples were prepared using TaqMan probes and the TaqMan Fast Advanced Master Mix (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... for 45 minutes at RT with gentle agitation and further digestion halted by addition of SUPERase:In (Ambion). Illustra Microspin Columns S-400 HR (GE healthcare ...
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bioRxiv - Microbiology 2024Quote: ... tuberculosis were used to perform RT-qPCR using Superscript III platinum-SYBR green one-step kit (Invitrogen). cDNA was generated from 50 ng of RNA using Superscript III platinum enzyme ...
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bioRxiv - Neuroscience 2023Quote: ... followed by donkey anti-rabbit secondary antibody (1:200 for 2hrs at RT; Thermofisher Cat#A-21206;) conjugated to a green fluorophore (AF488) ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was done with 0.5 µg total RNA using RT SuperScript III kit (Invitrogen, California, US) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: RT-qPCR: RNA was converted to cDNA using a High-Capacity cDNA Reverse Transcription Kit (ThermoFisher, # 4368814). qPCR was performed using a QuantStudio Real-Time PCR system (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: The cDNA was amplified RT-qPCR using Fast SYBR Green master mix (Thermo Fisher scientific, Waltham, MA) in a CFX96 Touch Real-Time PCR Detection System (BioRad ...