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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were subjected to fixation and permeabilization overnight using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, San Diego, CA, USA). The following day ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed and permeabilized using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set according to manufacturer instructions (Cat# 00-5523-00, Invitrogen, Waltham, MA). Cells were resuspended in Flow Cytometry Staining Buffer (R&D Systems ...
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bioRxiv - Developmental Biology 2023Quote: ... The dauer larvae were lysed using a Dounce tissue grinder set (Kimble 885300-0007) in PBS with Halt™ Protease Inhibitor Cocktail (100X) (Thermo Scientific 78429) until no intact worms remained ...
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bioRxiv - Cell Biology 2024Quote: ... unstitched high resolution (original pixel size = 8.2169 Å) TEM tile sets showing ultrathin sections of whole worm guts were examined via Maps software (Thermo Fisher Scientific Inc.) to detect all MVBs (per worm one section was analyzed) ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was conducted with a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using SYBR Green Master Mix (TaKaRa) ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies) using the TaqMan 2× Universal Master Mix and the following TaqMan Gene Expression Assays ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies) using the TaqMan 2× Universal Master Mix and the following TaqMan Gene Expression Assays ...
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bioRxiv - Genetics 2021Quote: ... The PCR was performed on cDNA templates using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, # 4368706) and primers specific for sequences in Pkhd1 exons 48-49 (forward ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Fluorescence was monitored on StepOnePlus Real-Time PCR System (Applied Biosystems ™StepOnePlus™Real-Time PCR System). To determine the critical threshold (Ct ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were screened through 1% agarose gel electrophoresis and purified using ExoSAP PCR Product Cleanup Reagent (Thermofisher). Amplicons were Sanger sequenced by Macrogen Europe Lab ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcriptase PCR (AppliedBiosystems #4368814) was performed to generate cDNA and Power SYBR green PCR mastermix (Applied Biosystems) was used for qPCR.
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was cloned into pJet1.2/blunt using CloneJET PCR Cloning Kit (K1231, Thermo Fisher Scientific, USA). The correct clone was selected by sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR was performed on Applied Biosystems 7500/7500 Fast Real-Time PCR System(Applied Biosystems, USA) using SYBR Fast Universal kit (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... Usp9x status was assessed by PCR using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific). Cycling conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR was carried out using the QuantStudio 12K Flex Real Time PCR System (ThermoFisher Scientific) with TaqMan Fast Advanced Master Mix (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... All quantitative PCR reactions were performed using QuantStudio-6/ViiA7/7900HT Fast Real-Time PCR System (Applied Biosystems) using 96-well or 384-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... and then subjected to real-time PCR analysis using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Genomics 2020Quote: ... Long PCRs were carried out in 50 µL reaction mixtures with 1x AccuPrime™ PCR Buffer II (Invitrogen), 1 U of AccuPrime™ Taq DNA Polymerase High Fidelity (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... Real-time quantitative polymerase chain reaction (PCR) was performed on QuantStudio 5 Real-Time PCR Instrument (Applied Biosystems). All experiments were done in triplicate with SYBR-GreenER pPCR SuperMix (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR reactions were carried out in the StepOne plus Real-Time PCR System (Applied Biosystems, United States) using FAST SYBR Green Master Mix (4385610 ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were amplified by real-time PCR (qPCR) using the Power SYBR Green PCR master mix (Applied Biosystems) on a CFX Connect™ Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Pathology 2019Quote: ... the PCR sample to test gene expression was prepared with SYBR Green PCR Master Mix (Applied Biosystems, USA), and the relative quantification 2 (-ΔΔCT ...
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bioRxiv - Biochemistry 2019Quote: ... qRT-PCR and data analysis were performed on the QuantStudio 12K Flex Real-Time PCR System (ThermoFisher Scientific). qRT-PCR was normalized using the geometric mean of the following internal control genes ...
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bioRxiv - Cell Biology 2019Quote: ... subcloned into pCR-BluntII-TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific, Waltham, MA) and verified by sequencing ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was performed with the SuperScript III Platinum Syber Green One-Step qRT-PCR Kit (ThermoFisher Scientific) using 25 ng of DNased RNA following the manufacturer’s instructions on a BioRad iQ5 cycler with melt curve analysis.
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bioRxiv - Genomics 2019Quote: ... PCR reactions were conducted using Platinum™ SuperFi™ Green PCR Master Mix (Cat. 12359010, Thermo Fisher Scientific). T7EI (Cat ...
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bioRxiv - Plant Biology 2019Quote: ... the same cDNAs were used in conventional PCR reactions using DreamTaq Green PCR Mastermix (Thermo Fisher Scientific, K1081) in a T-Personal thermal cycler (Biometra).
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bioRxiv - Microbiology 2019Quote: ... All transformants were screened by PCR using Phire Plant Direct PCR Kit (Thermo Scientific™: F130WH or F122S) and Taq DNA polymerase (NEB ...
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix with HF Buffer (Thermo Scientific) and primer concentrations of 0.5 µM ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time qRT-PCR was performed using power SYBR® green PCR master mix (Thermo Fisher, Waltham, MA), and an Applied Biosystems 7500 real-time PCR machine using a standard protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR (qPCR) was performed in triplicate on cDNA using SYBR® Green PCR Master Mix (Invitrogen, 4309155) using the QuantStudio™ 7 Flex Real-Time PCR System (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... Real-time PCR (qPCR) experiments were performed with a QuantStudio Real-Time PCR Light Cycler (Thermo Fisher Scientific). Transposon levels were quantified using the ΔΔCT method (Livak and Schmittgen 2001) ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative PCR was performed in a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the MicroAmp® fast 96-well reaction plate (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed as previously described (20) on a StepOnePlus Real-Time PCR System (Applied Biosystems). Relative quantity of transcripts was calculated as 2-ΔΔCt ...
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bioRxiv - Cancer Biology 2020Quote: ... Amplicons from genomic DNA PCR were ligated into vectors using the Zero Blunt TOPO PCR cloning system (ThermoFisher) and the presence of the desired mutations was validated using Sanger sequencing (GENEWIZ) ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR was carried out using an ABI Step One plus Real-Time PCR System (Applied Biosystems). Reactions for each sample were performed in triplicate ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: ... and All PCR analyses were conducted in triplicate using the 7900HT Fast Real-Time PCR System (Applied Biosystems). The primer sequences used to amplify the investigated genes listed in Supplementary Table 1.
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bioRxiv - Bioengineering 2021Quote: ... PCR was performed using Phusion DNA polymerase or Platinum™ SuperFi II Green PCR Master Mix (ThermoFisher Scientific). E ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Life Technologies, CA, USA). The cycling conditions were a polymerase activation step (95°C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, Life Technologies, CA, USA). The cycling conditions were a polymerase activation step (95°C for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... was used for qRT-PCR experiments on a Step-One Real-time PCR System (Thermo Fisher, Waltham, MA). Detailed steps are listed in Method S5.
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bioRxiv - Neuroscience 2019Quote: ... Quantitative real time PCRs (qRT-PCRs) were performed on 2 μl cDNA using SYBR Fast mastermix (Applied Biosystems) and specific primers for overall C9orf72 mRNA levels ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified with the HT ExoSAP-IT High-throughput PCR Product Cleanup (Affymetrix, Santa Clara, USA) following manufacturer’s instruction ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time quantitative PCR was performed using 2x Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific) to amplify 90 bp target with LINE primers (final concentration 200 nM) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA samples were amplified for two rounds by nested PCR using Platinum™ PCR Supermix High Fidelity (Invitrogen) and 5’ GeneRacer™ forward and reverse Tet1-isoform specific primers (Tet1FL 5’ RACE outside ...
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bioRxiv - Microbiology 2021Quote: ... The qRT-PCR was performed on an ABI QuantStudio™ 6 Quantitative Real-Time PCR System (Applied Biosystems) under the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... We conducted quantitative real-time PCR on ViiA7 Real-Time PCR System with QuantStudio software (v1.3) (Applied Biosystems) using PowerUP SYBR Green Master Mix (Applied Biosystems ...