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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: All PCR reactions were performed using the Phusion High-Fidelity PCR Master Mix (Thermo Scientific, Lafayette, CO, USA), and all diagnostic PCR reactions were performed using DreamTaq DNA Polymerase (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with DyNAmo ColorFlash SYBR Green Master Mix (Thermo Fisher Scientific) on a CFX96™ Real Time PCR System (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were amplified by PCR using DreamTaq Hot Start PCR Master Mix (Thermo Scientific, Waltham, Massachusetts, USA, #K9012) with the following primers targeting kcnb1 (10 mM) ...
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bioRxiv - Microbiology 2024Quote: ... Each PCR reaction contained 10 μl of DreamTaq™ Green PCR Master Mix (2X) (Thermo Fisher Scientific, USA), 0.5 μM of each primer (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... The qRT-PCR assays were performed in a QuantStudio 7™ Flex Real-Time PCR Systems (Applied Biosystems). Thermal cycling was performed at 25°C for 2 minutes ...
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bioRxiv - Cell Biology 2020Quote: Fixed HUVEC were blocked for 1hr at RT in blocking solution (5% FBS, 2X antibiotic-antimycotic (Gibco), 0.1% sodium azide (s2002-100G ...
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bioRxiv - Immunology 2021Quote: ... Samples from Biosafety Level 3 were inactivated for 2 hours at RT with 4% paraformaldehyde (ThermoFisher Scientific) after extracellular and intracellular staining.
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bioRxiv - Developmental Biology 2021Quote: ... for 5 min at RT and the signal was captured with X-ray film (Thermo Fisher, 34090) for 5-60 sec ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA was synthesized using 100-200 ng RNA with the Maxima H minus RT (Thermo Fisher, #EP0751) according to the manufacturer protocol and using random hexamers for priming ...
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bioRxiv - Molecular Biology 2020Quote: ... First-strand cDNA was synthesized using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR was performed using the SYBR Green (Maxima SYBR Green qPCR Master Mix, Thermo Fisher Scientific) on a C1000TM Touch Thermal Cycler (CFX96TM ...
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bioRxiv - Molecular Biology 2020Quote: ... the denatured RNA was used for the cDNA synthesis with a Superscript IV RT system (ThermoFisher, 18091050) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... stained for 2h at RT with secondary antibody Donkey Anti-Rabbit IgG – Alexa Fluor 555 (Life Technologies) 1/500 in PBST ...
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bioRxiv - Genomics 2020Quote: ... RT-qPCR for HOXA9 used 100 ng of RNA for cDNA synthesis (Applied Biosystems, Foster City, CA) and qPCR in duplicate in a StepOnePlus PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... The enzymatic steps (RT, RNA decontamination and ligation) were carried out in a Veriti Cycler (Applied Biosystems) according to the sequencing protocol ...
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bioRxiv - Cell Biology 2019Quote: ... GEM-reverse transcriptions (GEM-RTs) were performed in a Veriti 96-well thermal cycler (Thermo Fisher Scientific). After RT ...
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bioRxiv - Microbiology 2020Quote: ... burkhardae cells were stained 2 min at RT with 40 μg/mL AF488 NHS ester (ThermoFisher, Germany). Prior to mounting and STED microscopy ...
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bioRxiv - Genomics 2019Quote: ... The samples were cooled down to RT before loading them in Tris-Base 4-12% (Invitrogen NP0322BOX), then the gel was run in 1X MES-SDS running buffer (Invitrogen B0002 ...
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bioRxiv - Cell Biology 2020Quote: ... 2µg of RNA was used as substrate for first strand cDNA synthesis with Superscript III RT (Invitrogen). For PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were then placed on ice and reverse transcribed using the Superscript IV RT system (ThermoFisher, 18091050). For standard cDNA synthesis (no RNase R) ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS for 20 mins at room temperature (RT) and blocked with 10% goat serum (Life Technologies) for 20 mins at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then incubated for 10 minutes at RT with FM4-64 plasma membrane stain (#T13320, Invitrogen) and washed using M9 minimal media ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA was obtained via reverse transcription using the High Capacity cDNA RT Kit (Thermo Fisher Scientific # 4368814) and prepared for RT-PCR using the SYBR Green PCR MasterMix (Thermo Fisher Scientific # 4309155) ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 h at RT followed by Alexa Fluor 568-conjugated secondary antibodies (1:200 dilution, Invitrogen) for 45 min at RT ...
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Persistent DNA damage rewires lipid metabolism and promotes histone hyperacetylation via MYS-1/Tip60bioRxiv - Cell Biology 2021Quote: ... RT–qPCR was performed using diluted cDNA on a Quantsudio3 (applied biosystems) with PowerUp SYBR green (Invitrogen). Primers used in RT–qPCR are listed in supplementary table 2 ...
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bioRxiv - Pathology 2021Quote: ... RT-qPCR amplification reactions were carried out using the ABI Prism 7900 sequence detection system (Applied Biosystems) with an initial hold step (50°C for 2 min followed by 95°C for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... 5 U SUPERase•In™ RNase Inhibitor and 50 U SuperScript II RT (ThermoFisher Scientific, cat. 18064014). Reactions were incubated at 25°C for 10 min to allow partial primer extension ...
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bioRxiv - Cell Biology 2021Quote: ... For Reverse Transcription (RT) 500ng RNA was reverse transcribed High-Capacity RNA-to-cDNA Kit (Thermo Scientific). Each qPCR contained 5X diluted cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... The RT-qPCR assay was performed on cDNA (diluted 1:40) with SYBR GreenER qPCR SuperMix (Invitrogen) using QuantStudio7™ Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Synthetic Biology 2021Quote: Purified RNA samples were reverse transcribed (RT) into cDNA according to the manufacturer’s instructions (ThermoFisher Scientific, #4368814). qPCR of the RT cDNA was performed according to the manufacturer’s instructions in a LightCycler 480 II (Roche Life Science using SYBR GREEN (Roche Life Science ...
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bioRxiv - Genetics 2020Quote: ... Reverse-transcription was performed with the SuperScript™ III First-Strand Synthesis SuperMix for RT-qPCR (Invitrogen). RT-qPCR data was obtained from three biological replicates/siRNA treatment ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 to 2_μg of RNA was reverse transcribed with the high-capacity cDNA RT kit (Applied Biosystems), using random hexamers ...
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bioRxiv - Microbiology 2019Quote: ... and was used in reverse transcription (RT) reaction using 200 U of SuperScript III reverse transcriptase (Invitrogen). After RT ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out in SYBR™ Select Master Mix (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the total RNA was used for synthesizing cDNA with RevertAid RT Reverse Transcription Kit (Thermo Fisher scientific) under the guidance of manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 20 min at RT with 150 µL solution of 0.2 g/L NeutrAvidin (Thermofisher Scientific) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The reverse transcription (RT) reaction was carried out using SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen). Gene expression was measured by performing TaqMan PCR using Gene Expression MasterMix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... The RT-qPCR reaction used TaqPath 1-Step Multiplex Master Mix (catalog number A28527; Thermo Fisher Scientific) along with the 2019-nCoV RUO Kit (catalog number 10006713 ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesised from 500 ng total RNA using M-MLV reverse transcriptase (RT, ThermoFisher Scientific, 28025013) or Superscript IV reverse transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... We added 10 µl of a master mix with final concentrations of 1X RT Buffer (ThermoFisher Scientific), 10 mM MgCl2 ...
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bioRxiv - Physiology 2022Quote: ... -rat and –guinea pig secondary antibodies at RT for 1 hour on a nutator (1:500, ThermoFisher, #A21206 ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.5 μl of cDNA was used per RT-qPCR reaction with Power SYBR Green (Thermo Fisher) master mix ...
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bioRxiv - Cancer Biology 2022Quote: ... and reverse transcribed by using Maxima first strand cDNA synthesis kit for RT-qPCR with dsDNase (ThermoFisher) according to the manufacturers’ protocols ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific™) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... for 45 minutes at RT with gentle agitation and further digestion halted by addition of SUPERase:In (Ambion). Illustra Microspin Columns S-400 HR (GE healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 500ng of total RNA was used for reverse transcription (RT) using the Superscript III Reverse Transcriptase (Invitrogen). A minus RT reaction was performed for each sample and analyzed by qPCR for the expression of GAPDH (using GAPDH e9 primers ...
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bioRxiv - Immunology 2021Quote: ... 0.2 μM of probe and 1x TaqPathTM 1-Step RT-qPCR (Life Technologies, Thermo Fisher Scientific, Inc.). Amplification cycling conditions were ...
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bioRxiv - Immunology 2021Quote: ... 0.2 μM of probe and 1x TaqPathTM 1-Step RT-qPCR (Life Technologies, Thermo Fisher Scientific, Inc.). Amplification cycling conditions were ...