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Citations for Thermo Fisher :
7901 - 7950 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... followed by a 1:1000 dilution of HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (#62-6520, Invitrogen). Detection was performed with SuperSignal West Pico Chemiluminescent substrate (Thermo ...
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bioRxiv - Immunology 2021Quote: ... 1.75x103 cells were loaded into individual well of a 96 well v-bottom plate in RPMI/5% (v/v) FBS/2mM EDTA/1% (v/v) Penicillin/Streptomycin/1% (v/v) L-Glutamine (ThermoFisher)/0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... 0.5 μg of pTM1-L and 1 μg of pCI-T7opt were re-suspended in 250 μL of Opti-MEM (Gibco). The plasmid cocktail was then gently mixed with 15 μL of TransIT LT1 transfection reagent (Mirus) ...
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bioRxiv - Cell Biology 2020Quote: ... and Arp2 siRNA (Dharmacon, ON-TARGET plus SMART pool siRNA, catalogue no. L-011195-00-0005) using Lipofectamine RNAiMAX (Invitrogen) on day 1 and day 2 ...
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bioRxiv - Cell Biology 2021Quote: ... and HT-29 (HTB-38) were obtained from ATCC (Manassas, VA, USA) and cultured in Leibovitz’s L-15 Medium (11415064, Thermo Fisher Scientific/Gibco) supplemented with 10% FCS at 37°C without CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were maintained in culture (37°C, 5% CO2) fed by Neurobasal-A media supplemented with 2 mM L-glutamate (GlutaMAX, Gibco) 2 % B27 ...
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bioRxiv - Cancer Biology 2020Quote: ... obtained as formerly described [66]) were cultured in DMEM with 4 mM L-glutamine and 25 mM D-glucose (ThermoFisher) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Immunology 2021Quote: ... Co-expression of GFP and the CAR construct was verified by flow cytometry staining of the CAR using an anti-mouse IgG (H+L) antibody (A21235, Invitrogen).
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bioRxiv - Immunology 2021Quote: ... Dissected head kidneys were placed in 2mL of R-90 media (90% RPMI 1640 with L-Glutamine, without Phenol red; Gibco) in a sterile 24-well plate on ice ...
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bioRxiv - Cell Biology 2021Quote: ... authenticated) were grown in Dulbecco’s modified Eagle’s medium (DMEM) (high-glucose with L-glutamine and phenol red) (Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... serum reactivity to the Spike protein was measured using an HRP-conjugated goat anti-human IgG (H+L) (Invitrogen, 31410) at a 1:3000 dilution for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed 3 times and stained with a goat anti-hamster IgG (H+L) Alexa Fluor 488 (ThermoFisher Scientific). Cells were then fixed with 4% formaldehyde solution and fluorescence was evaluated on a LSRII analytic cytometer (BD Bioscience).
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bioRxiv - Immunology 2021Quote: ... polyclonal rabbit anti-Banf1 (Cat. PA5-20329) and goat anti-rabbit IgG (H+L)-Alexa Fluor 647 were purchased from ThermoFisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... pellets were resuspended in 50μl of 5% SDS containing phosphate buffered saline and cytoplasmic fractions were mixed 1:1 with 10% SDS solution (Thermofisher) to give a final 5% SDS concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthetized by in vitro transcription with 1μl of GTP added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Developmental Biology 2021Quote: ... the sections were washed and incubated with x1/2000 Alexa 488-conjugated anti-mouse IgG(H+L) (Thermo Fisher Scientific) or x1/500 ...
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bioRxiv - Cell Biology 2020Quote: NIH3T3 fibroblasts were grown in DMEM containing 4.5 g/L glucose supplemented with 10 % fetal bovine serum and penicillin/streptavidin (all ThermoFisher Scientific) at 37 °C in 5 % CO2 on non-coated tissue culture plates.
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bioRxiv - Evolutionary Biology 2021Quote: ... 1.7×107 cells were seeded into 175 cm2 cell culture flasks in RPMI1640 media with L-glutamine (Thermo Fisher Scientific) containing 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... 0.3 µL of Lipofectamine 3000 was diluted and well mixed into 10 µL of Opti-MEM I reduced serum medium (Gibco). In the other tube ...
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bioRxiv - Genomics 2020Quote: ... Immunoprecipitation of DNA-protein was performed using 4 µL of the polyclonal rabbit anti-Mcd1p (Covance Biosciences) overnight at 4°C or anti-V5 for Brn1p-V5 (Invitrogen). Antibody-bound lysates were incubated with 120 µL Protein A dynabeads (Invitrogen 10001D ...
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bioRxiv - Immunology 2020Quote: ... 2µl of cDNA was used in 12µl qPCR reactions with appropriate primers and SYBR Green PCR Master Mix (Applied Biosystems). Data were analyzed by ddCT method ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed using 2x ChamQ Universal SYBR qPCR Master Mix (Vazyme, L/N TE342F9) with QuantStudio3 machine (Thermofisher). The cycle procedure was at 50.0°C for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... the bacteria were grown overnight at 37°C with 240 RPM agitation in 5 mL of Lennox L broth (LB, Invitrogen) then diluted 1/20 in pre-warmed DMEM 25 mM Hepes (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-cleared by incubation with 20μL of IgG from rat serum crosslinked to protein-L Agarose beads (Thermo Scientific) for 2h at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies (1:1000) used were goat anti-mouse IgG (H+L) Alexa Fluor 594 (Thermo Fisher Scientific, A11005), goat anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2020Quote: ... they were crushed through a cell strainer and washed before being re-suspended in culture media (RPMI containing 10% FBS, Penicillin/Streptomycin, 0.1% β-ME, 20mM HEPES and L-Glutamine) (media and supplements from ThermoFisher) at the desired density of 107/ml of which 2.5×106 were used for subsequent flow cytometry experiments ...
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bioRxiv - Immunology 2021Quote: ... and cultured in tissue culture-treated petri dishes in complete medium (Dulbecco’s Modified Eagle Medium with high glucose and L-glutamine (Life Technologies) containing 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Tissue was shaken for 10 s before 5 ml of L-15 medium supplemented with 10% foetal calf serum (Invitrogen) was added ...
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bioRxiv - Immunology 2021Quote: ... or control rabbit IgG (Wako, 148-09551) for an hour followed by Alexa Fluor 594-conjugated donkey anti-rabbit IgG (H+L) (Invitrogen) for an hour ...
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bioRxiv - Immunology 2020Quote: ... The vRNA was eluted (45μL) and 5 μL was added in a 0.1 mL fast 96-well optical microtiter plate (Cat.# 4346906, ThermoFisher, CA).
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with siHMOX2 (Dharmacon L-009630-00-0005) or non-targeting pool (Dharmacon D-001810-10-05) siRNA using Lipofectamine RNAi Max (Thermofisher). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Capture plate wells contained 5 µl of capture solution (1:500 Phusion High-Fidelity Reaction Buffer, New England Biolabs; 1:250 RnaseOUT Ribonuclease Inhibitor, Invitrogen). Single cell libraries were then prepared using the previously described mcSCRB-seq protocol (18 ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were incubated with the FITC-conjugated goat anti-mouse IgG (H + L) secondary antibodies (1:300, Molecular Probes) for 1 h at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... L-glutamine 200 mM, Transwell® culture inserts (12 mm diameter, 0.4 μM pore size) and trypsin–EDTA from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Two days later the medium was refreshed and cells were either treated with 0.5 mM L-histidinol (228830010, Acros Organics), or 200 nM ISRIB ...
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bioRxiv - Neuroscience 2021Quote: ... the transfected cells were plated on poly-L-lysine-coated 35-mm round coverslip (#1.5 thickness) (Fisher Scientific, Waltham, MA). The cells were used for TIRF imaging 2 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-hamster IgG (H+L) HRP conjugated secondary antibody (SeraCare 5220-0371) was diluted 1:3,000 in casein in PBS blocking buffer (Thermo Fisher), and 100 μL was added to the wells and allowed to incubate for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM l-glutamine, 1 mM sodium pyruvate, 30% L929-conditioned medium, 20% heat-inactivated fetal bovine serum [FBS; Invitrogen] ...
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bioRxiv - Neuroscience 2022Quote: ZIKV isolated from a febrile patient in the state of Pernambuco, Brazil (BRPE243/2015, KX197192) was propagated in the C6/36 cell lineage cultured in Leibovitz L-15 medium (Invitrogen) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... washed with Coating Buffer and blocked with Cell culture medium (RPMI 1640) containing 10% Fetal Bovine Serum (FBS) and 1% Penicillin-Streptomycin-L-Glutamine containing 1% penicillin/streptomycin (GIBCO) for 2 h at room temperature.
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bioRxiv - Cell Biology 2022Quote: ... The cells obtained were cultured in complete medium (DMEM-F12, penicillin-streptomycin, L-glutamine, nonessential aminoacids, sodium pyruvate, all Gibco, Monza ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Cell Biology 2022Quote: The following secondary antibodies were used for immunofluorescence: AlexaFluor 488 goat anti-rabbit IgG (H+L) (Invitrogen, A11034, Lot: 2256692); AlexaFluor 594 goat anti-rabbit IgG (H+L ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... Entire 30 μL of both cellular and supernatant fractions were loaded on to a Bolt 4–12% Bis–Tris Plus Gels (Invitrogen). Details regarding membrane transfer and western blotting were documented previously (Zhou and Routh 2020) ...
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bioRxiv - Bioengineering 2022Quote: ... hMSCs were grown in alpha-minimum essential media (1×) (supplemented with L-glutamine, ribonucleosides, and deoxyribonucleosides) (Gibco, Cat. #12561-056) containing 20% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently probed with alexa fluor 488-conjugated anti-His or anti-mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (ThermoFisher), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... resuspended in ice-cold FACS buffer (2% v/v FBS, 50 mM EDTA in DMEM supplemented with high glucose, L-glutamine and HEPES, without phenol red [Gibco]), and incubated with 2 μg/mL of SARS-CoV-2 S RBD-IgG Fc for 1 h at 4 °C ...