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Citations for Thermo Fisher :
7851 - 7900 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... prior to differentiation cells were grown for at least 2 passages in 2i medium plus LIF (2i/L): DMEM/F12 (Gibco) supplemented with 0.5x N2 supplement ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were cultured in complete RPMI media (cRMPI; RPMI 1650, L-glutamine, 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... 20µl reactions to amplify the msp2 locus were prepared by mixing 0.02 units/μL of AmpliTaq DNA polymerase (ThermoFisher Scientific), 1x AmpliTaq Buffer II (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... Expi293F cells (Thermo Fischer Scientific) were seeded at 106 cells/ml density in 1 L of Dynamis growth medium (Gibco) 24 h before the transfection and supplemented with Tryptone/Glucose ...
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bioRxiv - Neuroscience 2022Quote: ... cells were resuspended in neuronal plating medium (NPM: Minimal Essential Medium with Earl’s Salt and L-glutamine, 0.6% D-glucose, 5% fetal bovine serum; Invitrogen, Carlsbad, USA) and seeded to a concentration of 3.0 × 105 cells per well on EM grids and glass bottom dishes (MatTek corp. ...
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bioRxiv - Neuroscience 2019Quote: ... Trypsin was neutralized with culture media (MEM, with L-glutamine, Phenol Red, without sodium pyruvate) supplemented with 10% horse serum (heat-inactivated, Gibco), 10 U/ml penicillin ...
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bioRxiv - Systems Biology 2020Quote: ... and the collection of progenitors from week 2 were immediately re-suspended in RPMI-1640 containing L-Glutamine (Life Technologies) and 10% Fetal Bovine Serum for macrophage differentiation (see macrophage differentiation).
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bioRxiv - Molecular Biology 2019Quote: ... 0.5 µL dideoxynucleotide and 0.2µL of the Platinum Taq DNA polymerase (PlatinumTM Taq DNA polymerase, Catalog number: 10966026; Thermo Fisher Scientific). Touchdown program was used in the Veriti 96 Applied Biosystems PCR thermal cycler and all samples were denatured at 95°C for 2min and 30sec ...
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bioRxiv - Cell Biology 2019Quote: ... 2 mM D-Glucose, 2 mM L-Glutamine, 1 mM Sodium Pyruvate, 140 mM KCl and 20 mM HEPES, Invitrogen) for longer imaging sessions ...
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bioRxiv - Neuroscience 2019Quote: ... the loosened tissues were collect and passed through a 40µm nylon mesh in 800µl resuspension buffer [Leibovitz L-15 with 0.3mM Glutamine (Gibco, Thermal Fisher, USA), 0.5% of Penicillin Streptomycin solution (Gibco ...
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bioRxiv - Systems Biology 2019Quote: ... cells were washed twice with PBS and then incubated in DMEM without glucose and pyruvate (0 g/l glucose, 0 mM pyruvate; Invitrogen) supplemented with 10% dialyzed FBS (Omega Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... immediately followed by addition of 100 µl of an isotonic medium based on Leibovitz’s L-15 medium (Gibco 21083-027) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: ... The suspension was shaken for 10 s before addition of 5 ml of L-15 medium supplemented with 10% foetal calf serum (Invitrogen) and centrifugation at 400 g for 5 min ...
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bioRxiv - Neuroscience 2019Quote: Brain capillaries were isolated from cortices of 8-20 weeks-old Cldn12-KO and WT mice by homogenization in Dulbecco’s modified Eagle’s medium (DMEM, 4.5 g/l glucose; Life Technologies, Darmstadt, Germany) with a dounce tissue grinder (Wheaton ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibodies were used at 1:1000 and included: goat anti-mouse IgG (H+L) dylight 488 (ThermoFisher Scientific, 35505); alexa fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2019Quote: ... containing anti-CD3 and anti-CD28 antibodies or isotype control antibodies (100 μg/ml) mixed with donkey anti-rabbit IgG (H&L)– Alexa Fluor 647 (10 μg/ml, Invitrogen) to visualize the spots ...
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bioRxiv - Bioengineering 2019Quote: ... or human organoid basal culture media consisting of conditioned media produced using stably transfected L cells (Wnt 50%; R-spondin 10%; Noggin 5%) and the following growth factors: B12 1X (Invitrogen), Nicotinamide 10mM (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... We used the following secondary antibodies conjugated with Alexa Fluor fluorochromes: 488 goat anti-guinea-pig IgG H+L (catalog #A11073, Invitrogen/ThermoFisher), 555 donkey anti-rabbit IgGs H+L (#A-31572) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on coverslips coated with poly-L-lysine in 6-well plates and cultured in Neurobasal medium (Gibco) supplemented with B27 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were resuspended in adhesion media (MEM, 10% Horse Serum, 30% glucose, L-glutamine and penicillin/streptomycin) (Life Technologies) and plated in 6-well plates coated with poly-L-lysine 0.1% w/v in water (Brunschwig ...
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bioRxiv - Bioengineering 2021Quote: ... where 2 × 105 HeLa cells were treated per electroporation using the Neon® TransfectionSystem 10 L Kit (Thermo Fisher Scientific) with the recommended electroporation parameters ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were grown on 10 cm tissue culture dishes in RPMI 1640 medium with L-glutamine (Thermo Fisher, Waltham, MA) and supplemented with 10% fetal bovine serum (ATCC ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 mg/l streptomycin and 3% bovine serum albumin (BSA) were pipetted into a 96-well optical bottom plate (Nunc) and centrifuged at 1,000 rpm for 3 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were labelled with 100 μl of a solution consist of 0.05 g/L NR dye and 2 droplets/ml NucBlue™ (Invitrogen) in UltraCULTURE™ by incubating for 3 h at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: Transformants were selected by plating first on YPD plates and then replica plating to yeast agar plates including 600 mg/L geneticin (Gibco) and incubating for a further 16 hours.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The 200 μL bacteria expressing dsRNA of LdCPR was spread on potato leaves in each petri dish (diameter = 8.5 cm, Fisher Scientific) to feed five female adults ...
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bioRxiv - Microbiology 2021Quote: ... and the re suspended in 50 μl ASB containing 0.1 μl Alexa 488 goat anti-rabbit IgG (H+L) (Molecular Probes). After 45 min ...
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... HEK 293T/ACE2 cells were first seeded in 96-well plates coated with 50 μg/mL poly-l-lysine at a density of 2×104/well in the culture medium described above but with GlutaMax (Gibco) added ...
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bioRxiv - Pathology 2021Quote: ... followed by nested or heminested PCRs (Taq Pegasus, PB-L Productos Bio Lógicos and/or Platinum™ Taq DNA Polymerase, Invitrogen). Initial screening was performed by using primers specific for the S segment as described [18] ...
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bioRxiv - Pathology 2021Quote: ... Polyclonal IgG H&L secondaries were Alexa Fluor 488- and 647-conjugates (Thermo Fisher #s A11029, A21235, A11008, and A21244) applied 1:500 in 10% ngs in PBST.
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bioRxiv - Molecular Biology 2020Quote: ... After washed three times with 1XPBS and incubated with Alexa Fluor 488 Goat anti-rabbit lgG (H+L) secondary antibody (1:500, Invitrogen) for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... After washed three times with 1XPBS and incubated with Goat anti-rabbit lgG (H+L) secondary antibody (1:500, Invitrogen) for 1 hours ...
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bioRxiv - Immunology 2020Quote: ... with or without the presence of peptides or anti-CD3 and inhibitors in a final volume of 200 μl RPMI1640 + L-glutamine (Gibco) containing 10 % FCS and penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Digested sera was probed with Rabbit anti-Human IgG-HRP H+L Secondary Antibody (ThermoFisher Scientific Catalog #A18903, 1:10,000), Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423 ...
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bioRxiv - Immunology 2021Quote: ... Fc Block was diluted in deficient RPMI (dRPMI; RPMI-1640 supplemented with HEPES and 1g/L sodium azide [ThermoFisher Scientific]) with 10% heat-inactivated FBS (dRPMI+FBS) ...
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bioRxiv - Immunology 2020Quote: ... was added to the cells in half culturing volume (40 μL added) and incubated in the dark for 5 min prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done on all ten mouse sera samples from each group in at least duplicate ...
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bioRxiv - Immunology 2020Quote: ... 4-incubation with 4 μg/mL secondary goat anti rabbit conjugated with AlexaFluor(AF)488 (goat IgG H+L, Invitrogen) in microscopy buffer for 1 h at RT ...
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bioRxiv - Immunology 2020Quote: ... gradient centrifugation and resuspended at 1-2 x 106 cells/ml in RPMI-10% FCS (RPMI 1640 w/L-glutamine (Cellgro Gibco) supplemented with 10% FCS (Cellgro Gibco) ...
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bioRxiv - Bioengineering 2019Quote: ... MDA-MB-231 and GFP-MDA-MB-231 cells were cultured in RPMI 1640 medium supplemented with 2 mM L-glutamine (Gibco), 10% FBS (Gibco) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1 µl of a 1:50 dilution of the first PCR product and 0.4 µl Long PCR Enzyme Mix (ThermoFisher Scientific). The PCR program was the same as for the first PCR ...
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bioRxiv - Microbiology 2019Quote: ... were seeded at 5×105 macrophages per well of a 24-well plate 24h prior to infection in (DMEM) with L-glutamine (2 mm) (Invitrogen). Overnight cultures of P ...
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bioRxiv - Cell Biology 2020Quote: ... OK) were maintained at room temperature (22°C) in 70% Leibovitz’s L-15 medium (ThermoFisher Scientific-Gibco, Cat#11415-064), 15% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2020Quote: ... OK) were maintained at room temperature (22°C) in 70% Leibovitz’s L-15 medium (ThermoFisher Scientific-Gibco, Cat#11415-064), 15% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2019Quote: ... PC3 and MX-1 cells were maintained in RPMI 1640 media and remaining cell lines in DMEM media supplemented with 2mM L-glutamine (Invitrogen) and 10% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... coverslips were mounted in magnetic chambers (Chamlide CM-S22-1, LCI) with cells maintained in L-15 medium without phenol red (Invitrogen) supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C on a heated stage in an environmental chamber (Incubator BL ...
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bioRxiv - Cell Biology 2019Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (AL007A, HiMedia, Mumbai, Maharashtra, India) supplemented with 10% FBS (10270-106, GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified lysates from His-tagged or dual tagged constructs were loaded onto an Nickel-nitrilotriacetic acid (Ni2+-NTA; 1.6 mL suspended beads/L growth) column (Thermo Scientific) equilibrated with 10 column volumes (CVs ...
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bioRxiv - Molecular Biology 2019Quote: ... exchanging half of the media with Serum-Free medium (DMEM w/o Phenol Red-GIBCO-, 25mM HEPES, L-Glu, Pen/Strep, 1% glucose, B-27 and N2 supplement-GIBCO-) every 2 days ...
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bioRxiv - Genomics 2021Quote: ... donovani promastigotes of the strain MHOM/NP/03/BPK282/0 clone 4 (further called BPK282, reference genome of L. donovani) were maintained at 26°C on HOMEM medium (Gibco, ThermoFisher) supplemented with 20% Fetal Bovine Serum ...