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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into HEK293T cells at a mass ratio of 2:1 (HC:LC) using Lipofectamine 2000 (Thermo Fisher Scientific). On day 3 post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with PBS and further incubated with 1:200 dilution of respective Alexa Fluor labeled F(ab’)2 fragment (Thermo Fisher Scientific Cat# A-11017 ...
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bioRxiv - Microbiology 2024Quote: Phage DNA was isolated by treating 2 mL of phage solution with 1 U/μL DNase I (Invitrogen, Carlsbad, CA, USA) to remove free DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of complementary DNA was performed using 1–2 μg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... the chitinous eggshells were first digested with base-bleach treatment (0.4 M KOH, 2% sodium hypochlorite [Fisher Scientific, catalog #SS290-1]) for 1.5 hours at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Then cells were transfected with the pre-incubated mixture of 1 µM 2-5A or 0.25 µg/ml poly I:C (Invitrogen, low molecular weight) and Lipofectamine 3000 (7.5 µl/6-well ...
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bioRxiv - Plant Biology 2024Quote: ... the protoplast-containing Trizol solution from PEG transformation was transferred to 2 mL Phasemaker tubes (1 mL per tube; ThermoFisher Scientific), mixed thoroughly with 300 µL chloroform ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split when they reached 80% confluency (every 2-4 days) using 0.5 mM EDTA in 1× DPBS (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated for 2 hours at RT with a mix of secondary antibodies: goat anti-chicken Alexa Fluor 647 (1:500, Invitrogen A32933) and goat anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Physiology 2023Quote: ... Samples were washed in PBST and incubated at room temperature for 2 hours with 1:200 Alexa Fluor 488- or 568-conjugated goat-species specific secondary antibodies (Molecular Probes) in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... followed by 2 h incubation with HRP-conjugated secondary antibody (1:10000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
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bioRxiv - Cell Biology 2023Quote: ... TMAs were transferred to humidity chambers and incubated for 2 hrs at RT with Alexa-Fluor-488 Wheat Germ Agglutinin (1:100; ThermoFisher, W11261) and Dapi (1µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane and immunoblotting was performed with the following antibodies: HA (#26183, 2-2.2.14, Invitrogen, USA, 1:1000), Myc (#2278 ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then incubated for 2 hours at room temperature with goat anti-rabbit Alexa 488-conjugated antibody (1:2000; A11008, Life Technologies). Sections were subsequently washed with PBS for 3 x 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... When hiPSCs were visually estimated to be 80% confluent they were passaged 1-2 times using Dispase II (2mg/ml; Gibco/Life technologies) in mTeSR1 and plated onto Matrigel coated plates ...
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bioRxiv - Biophysics 2024Quote: ... and desalted onto a trap column (1 mM inner diameter x 2 cm, IDEx C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific). Peptides were then separated onto a C18 analytical column (Waters Acquity UPLC BEH ...
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bioRxiv - Bioengineering 2024Quote: ... Tregs were stimulated for 24 hrs through the TCR (positive control) with anti-CD3/CD28-coated dynabeads (1 bead:2 cells ratio) (ThermoFisher Sci.) or through the HLA.A2-CAR with 2.5µL HLA-A2*02 dextramer (Immudex) ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Immunology 2023Quote: ... at a 1:2 ratio with irradiated (100Gy) CEMx174 cells in R10 (RPMI 1640 medium supplemented with 10% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: DNA illustration by agarose gel electrophoresis has been conducted by exploiting 2% agarose gel and 1X TAE buffer (1 mM EDTA and 40 mM Tris-acetate, Fisher Scientific) and by applying constant voltage of 120 V for 40 to 60 min ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then washed twice in 1% BSA/PBS and co-stained with 0.5mL 2 μM Hoechst 33342 (Thermo Fisher, USA) for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Microbiology 2022Quote: ... CXR dye and primers listed in supplementary table 2 at a final concentration of 1 μM on the StepOnePlus Real-Time PCR system from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Genetics 2023Quote: ... Animals were placed on a microscope slide with a 2% agar pad and immobilized with with ∼1 µl of 100 mM levamisole (ThermoFisher #AC187870100). A coverslip was applied and images for the same experiment were captured with identical exposure times.
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bioRxiv - Cancer Biology 2023Quote: ... F-actin was stained by incubating blocked slides for 2 hours at RT with Alexa Fluor 647 Phalloidin (1:400, Invitrogen #A22287).
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 2 hours with secondary antibody AlexaFluor 488 Goat Anti-Rabbit IgG (1:1000 in 4% BSA, Invitrogen #A11008) at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... then the wells were filled with 1 mL Neurobasal™ Plus Medium supplemented with 2% B-27 Plus supplement and 0.5% GlutaMAX™ all from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were immersed in xylene for 1-2 minutes and secured with coverslips using Cytoseal XYL mounting media (Thermo Fisher). Mounted slides were heated on a slide warmer for 2-3 hours at 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the glasses were incubated for 2 h at room temperature with secondary antibodies (1:800 Alexa Fluor 594 and 647 antibodies from Molecular Probes) and mounted in polyvinyl alcohol mounting medium with DAB-CO [52] ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 2 h at RT with AlexaFluor (AF) 488-labeled donkey anti-rabbit antibody (1:500, Invitrogen, Poland) and AF647-labeled phalloidin (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with the following fluorophore-conjugated secondary antibodies for 2 h at room temperature: anti-rabbit Alexa Fluor 488 (1:400, goat, Invitrogen, A11008), anti-rabbit Alexa Fluor 568 (1:400 ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.9 and 0.2M NaCl were heated for 5 min at 95°C and then cooled to 25°C in 2°C steps with 1 min per step in a thermal cycler (Applied Biosystems). The resulting double-stranded DNA (∼1 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 1 mL LB and added to a mixture of 2:1 methanol : chloroform (3.5 mL total) in borosilicate glass tubes (16×100 mm, Fisher Scientific 1495935AA). Samples were vortexed for 1 min to form a single phase ...
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bioRxiv - Immunology 2023Quote: ... The WIV was then dialyzed against 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered saline (Thermo Fisher Scientific, Bleiswijk, the Netherlands) at 4°C overnight to remove β-propiolactone ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Cell Biology 2023Quote: ... the plate was incubated at 65°C for 5 min and immediately chilled on ice for at least 2 min before adding 0.8 μL reverse transcription reaction mix [in 2 μL RT reaction: 1× First Strand buffer (Invitrogen – 18064-014), 20 mM DTT (Invitrogen – 18064- 014) ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM CHAPS) by centrifugation at 1000x g for 2 minutes with a micro-spin desalting column (Thermo Scientific Zeba) at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... detached cells were collected by centrifugation at 800g for 2 mins and red blood cells were removed by incubating the pellet with 1× red blood cell lysis buffer (Life Technologies) for 7 mins in RT ...
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bioRxiv - Microbiology 2023Quote: ... prior to a one-hour incubation with SARS-CoV-2 N protein antibody (clone 1C7C7) at a 1:300 dilution and a AF594-conjugated Goat-anti-mouse secondary (ThermoFisher, A11005). Slides were counterstained with DAPI and scanned on an Akoya Polaris Vectra imaging system ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 50 μL of a 1:4,000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid antibody (Invitrogen, Waltham, MA, Cat# MA536086) in blocking buffer was added and incubated for 2 h at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015), or electroporated into Hepa1-6 cells using a Neon Transfection System 10 µl kit (ThermoFisher MPK 1096 ...
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bioRxiv - Cell Biology 2023Quote: ... 1.2 × 106 cells were transfected with 1 μg DNA or shRNA for each construct using the Neon transfection system (Life Technologies) following the manufacturer’s instructions (1200 pulse voltage ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... Two sets of 100 μl of either Protein A Magnetic Beads (Thermo PI88846) (rounds 1 and 2) or Dynabeads MyOne Streptavidin T1 (ThermoFisher Scientific) (round 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). At 7 days post-transfection ...
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...