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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Young (feeding) L3 larvae were collected and fed overnight with standard flyfood containing EdU (5-ethynyl-2’-deoxyuridine) (Invitrogen) and Bromophenol Blue sodium salt (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... then each blastocyst was added to 5 µl of 2 × Reaction Mix (Invitrogen, CellsDirect One-Step qRT-PCR Kit) and snap-frozen on dry ice and stored at -80°C until processing.
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bioRxiv - Developmental Biology 2024Quote: The 5-ethynyl-2’-deoxyuridine (EdU) assay was performed using a click it-488 (C10337 and Click-it, Invitrogen) by soaking the dissected CNS for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 mL HisTrap column was washed with 10 column volumes of wash buffer (2× GIBCO 14200-075 PBS, 5 mM Imidazole, pH 7.5) followed by 6 column volumes of elution buffer (2× GIBCO 14200-075 PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 300 μm × 5 mm, 5 μm, 100 Å, separation column: C18, Acclaim PepMap, 75 μm × 500 mm, 2 μm, 100 Å, Thermo Fisher Scientific). After loading the sample on the pre-column ...
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bioRxiv - Immunology 2023Quote: Spleen cell suspensions were cultured for 5h in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ... washed 3x in PBS and subsequently for 2h with goat anti-rabbit 555 (1:1500) or donkey anti-sheep 647 (1:1500) respectively (ThermoFisher Scientific). Coverslips were mounted using Prolong gold with DAPI (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and inhibitors (50 µM 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA; Fisher Scientific), 8 µM EHop-016 (Selleck Chemicals) ...
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bioRxiv - Developmental Biology 2024Quote: ... aegypti embryos at specified developmental stages (0-1h, 4-5h, 5-6h, 23-24h, 47-48h, and 71-72h) using Trizol® reagent (Ambion by Life Technologies), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 anti-RUVBL2 rabbit polyclonal antibodies were used (HPA067966, Sigma-Aldrich: 1/500, incubation 32 min and PA5-29871, ThermoFisher Scientific: 1/50, incubation 32 min). Visualization was achieved with the Ultraview Universal DAB Detection Kit (Ventana Medical Systems) ...
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bioRxiv - Developmental Biology 2020Quote: Islet cell clusters were incubated (25 min at 37 °C) in RPMI supplemented with 2 μM Fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR). Fluorescence imaging was performed using a Nikon Eclipse TE2000-U microscope equipped with an epifluorescence illuminator (SUTTER ...
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bioRxiv - Microbiology 2021Quote: ... cells in each well were infected with 2000 TCID50 SARS-CoV-2 pseudovirus in 100 µL of culture medium containing 2% FBS (Gibco, Cat. No. 10099141C). Culture medium was refreshed every 12 hours ...
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bioRxiv - Immunology 2022Quote: ... and transferred using electrophoresis to nitrocellulose membranes by using the iBlot 2 Gel Transfer Device (Thermo Scientific, iBlot 2 NC Regular Stacks, IB23001, Invitrogen, Walham, MA). Membranes were blocked for 1 h at RT in 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Immunology 2021Quote: ... 200μL of platelet suspension (2×106 platelets in HBSSCa-Mg-) from healthy donors was labelled with calcein-AM (2 μg/mL final concentration, ThermoFisher Scientific Cat # C3100MP) for 15 mins and then R406 ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with 400 ng/well 2-E plasmids or 2-E mutations using Lipofectamine 3000 Transfection Reagent (L3000015, Thermo Fisher, MA, USA). The control group was Vero E6 cells transfected with 400 ng/well pcDNA3.1 vector plasmids alone ...
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bioRxiv - Developmental Biology 2023Quote: ... we washed the mature COCs in synthetic oviductal fluid medium (SOF) containing N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES-TL, Thermofisher Scientific, Waltham, MA) and SOF for fertilization (SOF-FERT ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Microbiology 2022Quote: ... and the residual bone marrow cells were plated in tissue-culture-treated microplates at a density of 2 × 105 cells per well in RPMI 1640 containing 2 mM glutamine (Thermo Fisher Scientific, 21875034), 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: To assess endothelium barrier function we perfused microvessels with 2 μM Alexa Fluor647-conjugated 2 MDa dextran (Thermo Fisher Scientific, cat. no. D22914) in EGM-2 medium 48 h following inoculation with Bb or vehicle (BSK-II medium ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Genetics 2024Quote: ... cell pellets were resuspended into PBS with 2% FBS and 1µg/ml of 4’,6-diamidino-2-phenylindol (DAPI) (Thermo Fisher Scientific, Cat#D1306) and transferred into 5 ml round bottom polystyrene flow tubes with cell strainer (Corning Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... and SARS-CoV-2 S protein were immobilized at 2 μg/ml on 96-well polystyrene microtiter plates Nunc MaxiSorp™ (Thermo Fisher Scientific). Following blocking with 1 × synthetic block (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were concentrated and washed onto a trap column (75 μm × 2 cm, 2 μm C18 resin, 100 Å; Acclaim PepMap100, Thermo Fisher Scientific) for 5 min with 2% ACN and 0.05% aqueous TFA at a flow rate of 30 µl/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies were from Dako (cat # P026002-2 and cat # P021702-2) and detected using either SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, cat # SKU:34579) or Western Lightning Plus-ECL Enhanced Chemiluminescence Substrate (Perkin Elmer ...
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bioRxiv - Developmental Biology 2024Quote: ... The pellet was then resuspended in 2 mL of MACS buffer (Hanks Buffered Salt Solution, 2% FBS, 2 mM EDTA) and incubated with streptavidin coated magnetic beads (Invitrogen MSNB-6002-74), spinning for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Experiments were conducted as described in our previous studies.(36) Neurons were loaded with the fluorescent calcium indicator Fura-2-acetomethoxyl ester (2 μg/mL, Molecular Probes, Eugene, OR) for 45 mins in the dark at room temperature and then allowed to de-esterify for 15 min at 37 °C in the warm external solution ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting peptic peptides were collected on a trap column (2 mm × 2 cm) that was filled with POROS 20 R2 material (Thermo Fisher Scientific, USA) and kept at 0.5 °C ...
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bioRxiv - Cell Biology 2024Quote: ... placed into individual wells in a 12 well suspension culture plate (greiner bio-one Cellstar, #665 102) containing warmed, oxygenated resting media (2 ml, RPMI 1640 with 2%FBS (GIBCOTM ThermoFisher Scientific, Cat# 11875093) and incubated overnight while on an orbital shaker.
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate, ThermoFisher Scientific) with a 6 mm diameter dropper ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 2 h at room temperature with the following primary antibodies: rabbit anti-EEA1 (1:3000, Thermo Fisher Scientific Cat# PA1-063A ...
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bioRxiv - Cell Biology 2020Quote: ... The Halo-H2A-expressing U2OS cell line was engineered to stably express NES-mCherry-LINuS (import probe) or NLS-mCherry-LEXY (export probe) by retroviral transfection and selection with 2 μg ml−1 blasticidin (#A1113903, Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: Cells were seeded at for screening at 1×105 cells per well of a 2 well Lab-Tek chamber slide (Thermofisher, 155360). The on-the-fly real-time capture was done using the 488 nm laser channel for excitation and using the 520 nm emission detector to collect the GFP signal and the 647 nm excitation laser and 667 nm emission detector for the segmentation channel ...
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bioRxiv - Physiology 2020Quote: ... frozen left-ventricular tissue was placed in Isolation solution (Supplemental Table 2) containing protease and phosphatase inhibitors (1:100 ratio, Fisher Scientific), 10 mM 1,4-dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were passed through an immobilized pepsin column (2 mm□× 2 cm) at 120 μL min−1 and the digested peptides were captured on a C18 PepMap300 trap column (Thermo Fisher) and desalted ...
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bioRxiv - Biochemistry 2021Quote: ... blotted for 1–2 s and plunge frozen into a liquid ethane/propane mixture with a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Developmental Biology 2020Quote: ... the ovaries were incubated for 2 h at room temperature with secondary Alexa 568-conjugated antirabbit IgG (1:500 in PBX; Invitrogen, Life Technologies), washed and counterstained with Hoescht (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells were incubated for one hour with the secondary fluorescent marker-conjugated antibodies at room temperature and counterstained with 1 μg/mL of 4’6-diamidino-2-phenylindole (DAPI, Molecular Probes, USA). Images were collected using the EVOS microscope ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and oligonucleotide primers (Eurofins Genomics, Louisville, KY; Tables 1 and 2) on a 7500 Real-Time PCR system (Thermo Fisher Scientific). For X ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed and then incubated for 2 hours at room temperature with biotinylated secondary antibodies (anti-rabbit 1:1000; Thermo Scientific) in the same buffer solution ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in PBT-X solution (2% NHS, 0.2% TritonX-100 in PB) with primary antibody (1:1000 polyclonal rabbit anti-GFP, ThermoFisher Scientific, #A11122) at room temperature for 24hr ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was washed with PB and incubated overnight in PBT-X (2% NHS, 0.2% TritonX-100 in PB) with secondary antibody (1:1000 AlexaFluor 488-conjugate anti-rabbit, ThermoFisher Scientific, # A27034). Tissue was washed with PB and mounted onto gelatinised slides ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in Alexa Fluor 488 donkey-anti-mouse (1:500 in TNB, Invitrogen, RRID:AB_141607). Following three washes with TNT ...
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bioRxiv - Neuroscience 2021Quote: ... 0.5% Triton X-100 in PBS) for 2 h at room temperature and then with primary antibody (rabbit anti-GFP, 1:1000, Invitrogen, G10362) diluted in blocking buffer overnight at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 hr incubation at RT in secondary antibody diluted 1:500 in block (goat anti-rabbit Alexa 568, Life Technologies; 1:500 goat anti-chicken Alexa 488 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated in PBT-X solution (2% NHS, 0.2% TritonX-100 in PB) with primary antibody (1:1000 polyclonal rabbit anti-GFP, ThermoFisher Scientific, #A11122) at room temperature for 24hr ...