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Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 hours at RT with appropriate secondary antibodies: goat anti-rabbit Alexa Fluor 633 (1:500, Invitrogen, A21070), and goat anti-mouse Alexa Fluor488 (Jackson Immunoresearch ...
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bioRxiv - Biochemistry 2021Quote: S9C:WT (1:2) αSyn amyloid fibres were incubated with Alexa Fluor 647 maleimide dye (10-fold molar excess, Thermo Fisher Scientific) and Biotin-X ...
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bioRxiv - Microbiology 2021Quote: ... 11 cDNA fragments with 70 bp end-terminal overlaps which spanned the entire SARS-CoV-2 isolate Wuhan-Hu-1 genome (GenBank accession: NC_045512) were produced by GeneArt™ synthesis (Invitrogen™, ThermoFisher) as inserts in sequence verified ...
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bioRxiv - Immunology 2020Quote: ... SARS-COV-2 Spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg SARS-CoV-2 S-2P or RBD) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
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Metal transporter SLC39A14/ZIP14 modulates regulation between the gut microbiome and host metabolismbioRxiv - Physiology 2021Quote: ... the pellets were resuspended in lysis buffer (Tris-HCl, 137mM NaCl, 10% glycerol, 1% Triton X-100, 2 mM EDTA) with protease and phosphatase inhibitors (Thermo Scientific) added along with the PMSF (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were rinsed three times in PBS and incubated with F(Ab’)2-goat anti-mouse IgG-Alexa 594 (1:500, #A11020, ThermoFisher Scientific) and DAPI (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were incubated for 2 hours at room temperature (RT) in carrier solution containing Alexa-488 conjugated secondary antibodies (1:200, Life Technologies). For phalloidin and DNase I treatment ...
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bioRxiv - Evolutionary Biology 2021Quote: ... (diluted 1:400 in 2% BSA) and a second step of 1 h of incubation with an Alexa Fluor 488 conjugate secondary antibody (Molecular Probes) (diluted 1:1000 in 2% BSA) ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 2 x 105 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 5% FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Immunology 2021Quote: ... OHSCs were washed and incubated with Alexa Fluor™ 488 donkey anti-rabbit IgG (2 μg ml−1; Invitrogen, A-21206) secondary antibody for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Cell Biology 2021Quote: ... The secondary antibody was F(ab’)2-Goat anti-Rabbit IgG conjugated with Alexa Fluor 350 dye (Invitrogen, 1:500 dilution).
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bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...
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bioRxiv - Cancer Biology 2021Quote: ... an additional 100 μL of EGM-2 without growth factor was added with 1.5 μL of 1 mg/mL calcein-am (ThermoFisher Cat # C1430). Cells were then imaged 30 minutes later at the 4-hour timepoint ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (beads/cell ratio of 1/2; Gibco, Thermo Fisher Scientific) or with 4.5 μg/mL phytohemagglutinin (PHA ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... samples were homogenized and ∼15g was diluted 1:2 (w/v) in cold phosphate-buffered saline (PBS; Gibco, ref.:10010-031), vortexed for 1 minute and centrifuged at 4000xg for 15 minutes at 4°C instead of 3000xg ...
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bioRxiv - Developmental Biology 2022Quote: Animals injected with either shRNA #1 or #2 were collected at 48hpf and lysed in 500 µl of TRIzolTM reagent (Invitrogen, 15596026) by vortexing extensively ...
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bioRxiv - Cell Biology 2022Quote: ... islets from Pdx1-CreERTg/0 Rosa26mTmG/- transgenic mice were washed 3× in room temperature PBS + 2 mM EDTA and then dispersed by constant gentle pipetting in 1 mL of 0.025% Trypsin-EDTA (Thermo Fisher Scientific) in PBS at room temperature for 8-10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Lungs were inflated with 1 ml of a solution containing 1 part 4% PFA/30% sucrose and 2 parts of optimal cutting temperature compound (OCT) media (Fisher Scientific). Inflated lungs were tied off and stored in 4% PFA/30% sucrose at 4°C overnight ...
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bioRxiv - Molecular Biology 2022Quote: C2C12 cells were plated onto 6-well plates at a 2 x105 density and 24 h later transfected with either negative control siRNA (Negative Control No.1 siRNA, Life Technologies) or custom siRNAs targeting S1P (Silencer Select siRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Biochemistry 2022Quote: ... then placed in fresh ice-cold HBSS and cut into small pieces to be digested in prewarmed HBSS with 1 mg/ml Collagenase Type 2 (Life Technologies) at 37 °C for 5 min with gentle agitation ...
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bioRxiv - Bioengineering 2022Quote: ... and calcium-sensitive dye Rhod-2 AM (1 mg/ml dye stock solution in DMSO, Thermo Fisher Scientific, catalog number R1244). The heart was subjected to a 15 min equilibration period followed by dye staining to allow the washout of extra dye ...
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bioRxiv - Cancer Biology 2022Quote: ... The stock solutions of the agonists were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific) and the enzyme inhibitors were reconstituted according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using conditions recommended by the supplier (1× PNK buffer without ATP, 2 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 2 h) ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... Total lipids were isolated by Folch extraction116 by adding 24 mL 2:1:0.6 HPLC chloroform (C297-4, Thermo Fisher Scientific): HPLC methanol (A454-4 ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 µL of buffer containing 20 mM of NH4Ac at 42 degrees for 2 hours followed by phosphatase treatment using 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) at 37 degrees for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with rabbit anti-goat IgG conjugated with Q-dot 655 for 2 h at 4 °C (1:350 dilution; Life Technologies). Then ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours later the slices were washed three times in PGT following by an incubation of 2 hours on room-temperature with the following secondary antibodies: anti-rabbit 488 (donkey, 1:1000, Life Technologies), anti-goat 568 (donkey ...
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bioRxiv - Neuroscience 2022Quote: ... pooled from 3 pups were seeded per well on a Seahorse XF96 plate and cultured for 14 days in neuron growth medium (Neurobasal Medium, 2% B27, 0,25% Glutamax, 1% Penicillin/Streptomycin; all Thermo Fisher Scientific) with the different treatments before performing the Seahorse run.
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bioRxiv - Molecular Biology 2022Quote: ... The grids were washed by dipping 2 times in PBS (37°C) before 3 μL of 1 μm Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... After rinsing three times with TBS + 0.2% Triton sections were incubated with secondary antibodies donkey anti-rabbit Alexa Fluor 647 (1:500, Cat# A-31573, ThermoFisher, RRID:AB_2536183) and goat anti-chicken Alexa Fluor 488 (1:500 Cat# A-11039 ...
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bioRxiv - Immunology 2022Quote: ... were transfected with Sanger sequencing-verified SARS-CoV-1 or variant-specific SARS-CoV-2 S expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten- micro-liter of the aliquot was mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher). The resulting RNA samples were fragmented ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 hour probing with the appropriate secondary antibodies diluted in block: Anti-guinea pig IgG-Alexa 594 (1:250, ThermoFisher #A11076), anti-rat IgG-Alexa 488 (1:250 ...
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bioRxiv - Plant Biology 2024Quote: ... Immunolabelling experiments were performed with LM19 and LM20 (1:10 dilution in TTBS-milk, 2 h incubation) followed by anti-rat IgG-Alexa488 (Invitrogen®) or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438 ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...