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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 (15 min) and stained for 30 min with 2 μg/ml HCS Cell Mask stain (Life Technologies). Syncytia were counted microscopically in triplicate wells ...
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bioRxiv - Genomics 2022Quote: ... resuspended in ice cold PBS+BE (1 mg/mL BSA + 2 mM EDTA) and incubated for an hour at +4°C with Dynabeads Biotin Binder (Thermo Fisher). Cells were repeatedly washed with ice-cold sterile PBS while on magnet ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated at 37 °C and images were taken manually every 1 h for 24 h using Evos FL Auto 2 inverted microscopy (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: ... cells were incubated with 20-50 nM SiR-tubulin dye (SpiroChrome) for 1-2 hours and transferred to L-15 medium (ThermoFisher Scientific) before imaging ...
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bioRxiv - Biochemistry 2022Quote: 250 μg of protein from HEK-293T cell lysates were incubated overnight with 2 μg of a 6x-His tag antibody (MA 1-135, Thermo Fisher) in the presence of a low–salt RIPA buffer (20mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated at room temperature for 2 hours along with Alexa Fluor 488 phalloidin (Invitrogen #A12379, 1:40 dilution). Secondary antibodies were removed ...
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bioRxiv - Molecular Biology 2022Quote: ... DHIV3-GFP and env-expression plasmids were co-transfected at a mass ratio of 2:1 using lipofectamine 2000 (ThermoFisher, 11668030). shRNA encoding lentiviral particles were produced by co-transfecting pHIV7 ...
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bioRxiv - Plant Biology 2022Quote: ... was used for floral dip transformation of pTAN1:CFP-TAN1 and selected on 1/2 MS plates containing 100 μg/mL gentamicin (Fisher Scientific). T1 seedlings were screened for mScarlet and CFP signal and then transferred to soil to self.
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Cancer Biology 2022Quote: ... confluent stromal cells were stained for 10 minutes with 2 μM MitoTracker Green FM and 1 μM MitoTracker DeepRed FM (Molecular Probes), washed twice ...
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bioRxiv - Cell Biology 2022Quote: Wild-type HeLa cells (HPA/ECACC 93021013) or FCHO1/2 KO HeLa #64/1.E (Umasankar et al., 2014) were cultured in DMEM with GlutaMAX (Thermo Fisher) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Developmental Studies Hybridoma Bank) and secondary antibody goat α-mouse F(ab’)2 fragments labelled with Alexa Fluor647 (1:500, A21237, Thermofisher). Boutons were visualized using Alexa Fluor488 conjugated goat α-horseradish-peroxidase antibody (α-hrp ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated for 2 hours at room temperature protected from light in fluorescently conjugated Alexa Fluor secondary antibodies 1:500 (ThermoFisher Scientific) and DAPI (D3571 ...
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bioRxiv - Neuroscience 2021Quote: ... and rinsed and then incubated for 2 h with appropriate Alexa Fluor-conjugated secondary antibodies (R37117, Thermofisher, Waltham, MA, 1 : 500). Fluorescent images were acquired using Olympus IX81 or Zeiss 880LSM microscopes.
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bioRxiv - Neuroscience 2021Quote: ... in 2% NHS / 0.4% Triton™ X-100 in PBS with primary antibodies against Pax2 (1:500, rabbit, Invitrogen 71-6000), Calbindin D28-K (1:10.000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfection solution was prepared by adding 1 μg/ml purified DNA and 2 μg/ml linear PEI in Serum- Free Hybridoma Media (Gibco®) supplemented with 1 % FCS ...
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bioRxiv - Immunology 2021Quote: ... and then 1-2 μg of total RNA was used to prepare cDNA using high-capacity cDNA Reverse Transcriptase kit (Applied Biosystems). Quantitative PCR was performed using SYBR Green master (Roche ...
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bioRxiv - Microbiology 2021Quote: ... and incubation at 37°C for 1 hr and 2) 1 hour incubation at 50°C following addition of 15μl Proteinase K (20 mg/ml, Thermo Scientific™) and 30μl Proteinase K digestion buffer (0.1M NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Micropost arrays were passivated using a 1:2 mix (0.5 mg/ml in PBS) of fluorescently labeled (DyLight 405 NHS ester; Fisher Scientific Ireland) endotoxin-free bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The liquid portion of the sample was removed and diluted 1:2 by volume in sterile PBS without calcium or magnesium (Gibco, 10010023). The sample was spun again at 3000 rpm for 5 minutes at RT ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells stably expressing TfR-GFP were plated on fibronectin coated MatTek dishes and the next day incubated with 1 μM Alexa 647 labelled AP3 aptamer in blocking buffer (2 mM salmon sperm DNA, ThermoFisher #15632011), 2 mM yeast tRNA (ThermoFisher #AM7119 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then washed three times in 0.1 M PBS and incubated for 2 h at room temperature in donkey anti-goat AlexaFluor546 (1:500, Invitrogen, #A-11056), donkey anti-goat FITC (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours with the secondary antibody (Alexa fluor™ 488 goat anti-rabbit 1:1000, Invitrogen), mounted on slides and embedded in fluoromount.
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with wash buffer (DPBS containing 1% FBS) and incubated with 2 µg/ml of streptavidin-PE-Cy7 conjugate (Life Technologies) on ice for 30 min and then washed with wash buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Captured cells were stained with 2 μM green-fluorescent calcein-AM and 4 μM red-fluorescent ethidium homodimer-1 (Molecular Probes) and visualized under an EVOS FL cell imaging station (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2 h at RT with an Alexa Fluor 555 nm-conjugated goat anti-mouse secondary antibody (1:500; Molecular Probes, ThermoFisher Scientific cat#A21127 ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Immunology 2022Quote: ... The three Fabs were expressed by co-transfection of plasmids of heavy and light chain at the ratio of 2:1 in ExpiCHO expression system (Life Technologies) for two weeks according to the manufacturer’s manual ...
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bioRxiv - Immunology 2022Quote: ... Sections were then incubated with cocktails of primary antibodies: rabbit anti-SARS-CoV-2 Spike Protein (1:100, Invitrogen, #MA5-36087) + rat anti-ERα H222 (1:100 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in 1xPBS supplemented with 2 % fetal bovine serum (FBS, Hybclone) and 1 μg/mL 7-AAD (Life Technologies), filtered through a 40 μm strainer ...
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bioRxiv - Immunology 2020Quote: ... in PBS/0.5% BSA at room temperature for 2 h and washed in PBS/0.2% Tween-20 at room temperature for 1 h five times before mounting with SlowFade Diamond Antifade Mountant (S36963, Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
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bioRxiv - Biophysics 2020Quote: ... 1 μL of 20 mM latrunculin A or 1 μL of DMSO was mixed with 100 μL of YE5S medium containing 2% (w/v) agarose UltraPure agarose (Invitrogen Corporation) kept in a water bath at ~70 °C ...
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bioRxiv - Microbiology 2021Quote: For GFP RIPs HEK-293Ts were transfected with 2μg GFP or GFP-ORF57 plasmids with DNA in a 1:2 ratio with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... Cells were further washed three times with washing buffer (0.02-0.01% Triton X-100 + 0.3% BSA in PBS) and incubated with 2° Ab at 1:1000 dilution (Goat Ab-AF 594, Life Technologies, A11005) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with TBST and incubated in secondary antibody (2% NGS in TBST) with Alexa 568-conjugated goat anti-rabbit (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes were washed the following day with TBS and incubated in secondary antibody solution (2% NGS in TBST) with goat anti-mouse 488 (1:5000, Thermo Fisher Scientific Cat# A-11001 ...
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bioRxiv - Microbiology 2020Quote: ... and beads were washed 5x 2 minutes in 1 mL of ChIRP Wash Buffer (2x NaCl-Sodium Citrate (SSC, ThermoFisher Scientific), 0.5% SDS ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA samples were prepared from freeze-dried cells (CHRIST Alpha 1‐2 LDplus lyophilizer, Osterode, Germany) derived from untreated and farnesol-treated cultures using TRISOL (Invitrogen, Austria) reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 555 donkey anti-mouse (1:1000, Life Technologies-Invitrogen). Cells were finally stained with DAPI (1:5000 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... in a RNase/DNA-free fume hood 2 µl of RNA was mixed with 1 µl of dNTP mix (Thermo Scientific), 1 µl of Oligo-dT primer (Merck ...
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bioRxiv - Immunology 2020Quote: ... The isolated CD8+ T cells were washed and resuspended at a concentration of 1 × 106 cells per ml in completely reconstituted RPMI supplemented with 50 units/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads (Life Technologies) per ml ...
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bioRxiv - Cell Biology 2020Quote: ... and either pINDUCER-20-IRS2 or pHAGE-FLAG-HA-NTAP-IRS2 in a 4:2:1 DNA ratio using Lipofectamine 3000 (Invitrogen, L3000001) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were resuspended in buffer (PBS/2% FCS/ 1% penicillin/streptomycin) containing a viability dye from the Sytox series (Life Technologies) or DAPI (Sigma) ...