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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the media was removed and cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) at a concentration of 1 µg/mL (ThermoFisher Scientific) in Phosphate-Buffered Saline (PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... the plate was washed three times and incubated for 1 h with 100 μL of F(ab’)2-goat anti-human IgG (H+L) antibody conjugated to HRP (1:5,000 dilution; ThermoFisher, Cat # A24464). After three washes ...
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bioRxiv - Genomics 2024Quote: ... then incubated for two hours at room temperature with donkey secondary antibodies diluted in PBS with 2% NDS and 0.1% Triton X-100 as follows: anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, RRID AB_141607), anti-guinea pig Alexa Fluor 647 (1:300 ...
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bioRxiv - Immunology 2024Quote: ... Spleen and lymph nodes were collected from euthanised mice and macerated through 40 μm filters using PBS supplemented with 2% fetal bovine serum and 1 mM EDTA (Invitrogen, AM9260G). CD8+ T-cells were isolated from the single cell suspension using the EasySep Mouse CD8+ T-cell isolation kit (Stem Cell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... then wildtype HeLa or HEK293T-H-Kb cells were transfected with the plasmids using Lipofectamine 2000 in a ratio of 2:1 reagent:DNA (Life Technologies, 11668500). 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA ID: s6696, s6697 and s6698 for HNF4A and cat# 4390843 and 4390846 for negative control #1 and #2, respectively, Thermo Fisher) and gene expression analysis was performed as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... pre-REP TIL were co-cultured with 2×107 40 Gy-irradiated allogeneic feeder PBMC (1:200 ratio) in upright 25-cm2 flask (ThermoFisher Scientific) in REP-TIL culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Physiology 2024Quote: Isolation of ADSC-derived small extracellular vesicles (sEVs) involved culturing ADSCs in high-glucose DMEM with 2% exosome-depleted FBS and 1% penicillin/streptomycin (Gibco, A2720803) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 15 min in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) solution (1:5000; catalog no. D1306, ThermoFisher Scientific, USA), followed by four 15-min washes in PBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... and rinsed and then incubated for 2 h with appropriate Alexa Fluor-conjugated secondary antibodies (R37117, Thermofisher, Waltham, MA, 1 : 500). Fluorescent images were acquired using a Nikon A1R+ laser scanning confocal microscopy system on a Nikon Ti2-E inverted microscope ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol for a 25 mL culture ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times for 15 min in PBS before a 2 h incubation with either a donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (dilution 1:500, Invitrogen, #A21208) or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500 ...
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bioRxiv - Microbiology 2024Quote: MDCK or MDCK SIAT1 cells were seeded in 96-well plates at 1-2 ξ 104 cells/well in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... and their bladders were excised and placed into 2 mL conical-bottom screw-cap tubes (USA Scientific #1420-8700) on ice containing 1 mL TRIzol (Invitrogen #15596018) and six 2.8 mm diameter ceramic beads (Omni International #19-646) ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected with Glod4-targeting siRNAs (siRNA Glod4 #1: Cat. #43390816 ID s84641; siRNA Glod4 #2: Cat. #4390816 ID s84642, Thermo Scientific); siRNA scrambled (siRNAscr ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were washed with wash buffer (0.05% Tween 20 in PBS) and blocked with 2% BSA (Biowest) in PBS containing 1% Casein blocker (Thermo Fisher Scientific) for 1 h at RT ...
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bioRxiv - Plant Biology 2024Quote: ... the protoplast-containing Trizol solution from PEG transformation was transferred to 2 mL Phasemaker tubes (1 mL per tube; ThermoFisher Scientific), mixed thoroughly with 300 µL chloroform ...
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bioRxiv - Neuroscience 2024Quote: ... DPBS was removed and cells were crosslinked by adding 1 mL pre-warmed PBSx1 supplemented with 2 mM disuccinimidyl glutarate (DSG, ThermoFisher, #20593) and protease inhibitors for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... All cultures received N2 medium from d0 to 11 (50% MACS Neuromedium, 50% DMEM/F12 containing 1% N-2 supplement [Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with secondary antibodies for 2 hours at RT: Alexa Fluor 647 anti-guinea pig (Invitrogen, A21450; 1:200), Alexa Fluor 488 anti-rabbit (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBST then incubated for 2 hours in blocking solution plus secondary antibodies: goat anti-rabbit 568 (1/500, Invitrogen, A11011) and goat-anti guinea pig 633 (1/500 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed three times in PBS and incubated for 2 hours with the secondary antibody Alexa Fluor 488 Goat-anti-Chicken IgG (1:1000, Invitrogen, A11039) and DAPI (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with plasmid coding for eIF2A-Flag using Lipofectamine 2000 in a ratio 1:2 DNA:reagent (Life Technologies, 11668500). One day later ...
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated for 1 hour in blocking solution containing the DNA dye Hoechst 33342 (Invitrogen; 2-10 µg/ml), Alexa Fluor 555-conjugated secondary antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated for 1 hour at room temperature with secondary antibodies diluted in 1XPBS with 2% goat serum (AlexaFluor-594 anti-mouse Thermo Fisher Scientific #A11032 ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI was allowed to dry for approximately 3 minutes before immediately coverslipping with 1-2 drops of ProLong Gold (Invitrogen, P36930). Slides were protected from any surrounding light ...
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bioRxiv - Microbiology 2024Quote: ... the cells were washed once with PBS and 3 mL of DMEM (2% FCS) containing 1% low melting point agarose (ThermoFisher Scientific) was added to each well ...
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bioRxiv - Neuroscience 2024Quote: ... or 2% normal goat serum in 0.2–0.3% Triton X-100 in PBS: anti-human tau (clone HT7) (ThermoFisher, MN1000; 1:50), anti- MAP2 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested protein was diluted 1:2 v/v with water and protein concentration was determined using a Pierce BCA assay kit (ThermoFisher Scientific)
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bioRxiv - Neuroscience 2024Quote: ... Biopsy punch samples 200 – 400 μm thick were dehydrated in an ascending series of ethanol dilutions in water (50%, 70%, 90%, and 2 x 100%) containing 1 mM dithiothreitol (DTT; ThermoFisher Scientific) for 40 seconds per step with the temperature restriction at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Nuclei and F-actin were stained by incubation with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific) and phalloidin–tetramethylrhodamine B isothiocyanate (phalloidin-TRITC ...
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bioRxiv - Biophysics 2024Quote: ... 50 nM of fuel DNA strands 1 and 2 in 1xTE buffer containing 750 mM NaCl and 100 nM anti-Dig antibodies (Rb Monoclonal, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The remaining 2 mL aliquot of 1% acetic acid was combined with glutaraldehyde (50% aq. Soln, Thermo Scientific Chemicals, Waltham, Massachusetts) to produce a 1% glutaraldehyde solution ...
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bioRxiv - Bioengineering 2024Quote: ... and transmembrane serine protease 2 (TMPRSS2) were seeded at a density of 1×104 cells/well in white-walled 96-well plates (ThermoFisher Scientific). The following day ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with 2 μg mL-1 Alexa Fluor Plus 647-conjugated donkey anti-rabbit IgG [H+L] and 2 μg mL-1 Hoechst 33342 dye (Invitrogen 62249) for 1 h to label non-internalized beads and DNA ...
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bioRxiv - Bioengineering 2024Quote: ... Mouse syngeneic Py8119 breast cancer cells suspended at 1–2 million/150 μL of 33% (v/v) Matrigel (Thermo Fisher Scientific) were orthotopically injected into 6-8-week-old female C57BL mice (Jackson Laboratories) ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were resuspended in 100 µl of 1× DNase buffer and incubated with 2 units of DNase (TURBO, Thermo Fisher Scientific) at 37 °C for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were then transferred to a 1:2 mix of 30% sucrose solution and optimal cutting temperature (OCT, 23-730-571, Fisher Scientific) for about 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The following commercial antibodies were used: Na/K ATPase (ATPA1 mAb M7-PB-E9, Invitrogen #MA3-928, 1-2 μg/ml), α-Tubulin (mAb DM1A ...
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bioRxiv - Cancer Biology 2024Quote: OVCAR4 cells expressing scramble control or MPZL3 shRNAs (sh #1 and sh #2) were stained with CellTrace Far Red (Invitrogen, C34572), seeded into 96-well ULA plates (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... OVCAR4 cells expressing scramble control or MPZL3 shRNA (sh #1 and sh #2) were stained with CellTrace Far Red (Invitrogen, C34572) and added as a suspension to the pre-seeded 96-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... They were then washed and incubated 2 hrs in Alexa Fluor 647-conjugated donkey anti-rabbit IgG antibody (1:2000, Invitrogen: A31573). The membranes were then imaged to detect for fluorescence using a gel imager (Bio-Rad ChemiDoc MP).
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bioRxiv - Cell Biology 2024Quote: ... The PA gel surface was functionalized with 1 mg/ml of Sulfo-SANPAH (sulfosuccinimidyl-6-(4-azido-2-nitrophenylamino) hexanoate) in warm 50 mM HEPES buffer (Life Technologies) for immobilization of 100 μg/ml collagen type I (PureCol ...
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bioRxiv - Cell Biology 2024Quote: ... the slides were washed in 1 x PBS and covered with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images for Protein A fusion proteins were obtained using camera Olympus DP73 (Axioplan 2 imaging) ...
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bioRxiv - Developmental Biology 2024Quote: ... The hemi-thoraxes were incubated 2 hours in 24-well plates for actin staining with phalloidin rhodamine (1/500, Invitrogen R415) or GFP nanobody (1/2000 ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilised with 0.2% Triton-X in PBS-Ca2+ and blocked for 1 hour at RT in 2% FBS in PBS-Ca2+prior to incubation in Alexa Fluro-conjugated secondary antibody (1:000 dilution) (Thermofisher, A32728). Slides were mounted onto coverslips and imaged were obtained with a Leica Stellaris 8 microscope using a 64 x oil immersion objective and sequential excitation at wavelengths of 405 ...
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bioRxiv - Bioengineering 2024Quote: ... Methanol-d4 (CD3OD) was purchased from ACROS Organics. NaOD in D2O was purchased from Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...