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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was obtained from Life Technologies (Carlsbad, CA) and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Biophysics 2024Quote: ... and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Thermo Scientific, 24510) in Borate buffer (100 mM Borate-NaOH ...
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bioRxiv - Microbiology 2024Quote: ... was mixed with the internal standards (ISTDs) d4-CDCA and d4-LCA (10 µM each) and dried in a SpeedVac system (Jouan RC10.22, ThermoFisher Scientific, USA). The residue was dissolved with methanol and water [1:1] ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Biophysics 2021Quote: ... or a combination of pepsin and fungal protease type XIII (manually conjugated to POROS™ 20 AL Aldehyde Activated Resin, Thermo Fisher Scientific, and packed in a 2 mm ID x 2 cm IDEX C-130B column, Fisher Scientific, Hampton, NH), loaded on a C18 analytical column (Hypersil Gold ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies of strains were inoculated into 2 mL of minimal medium containing 2% succinate and 50 µg/mL kanamycin in 14 mL polypropylene culture tubes (Fisher Scientific, Waltham, MA, USA), then grown to mid-exponential growth phase with shaking at 200 rpm and 30 °C on an Innova 2300 platform shaker ...
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bioRxiv - Microbiology 2021Quote: ... The cell monolayers were then blocked with blocking buffer (2% Blotting-Grade Blocker [Bio-Rad] supplemented with 2% goat serum [Gibco] in PBS-Tween) for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... iAT2s were serially passaged approximately every 2 weeks by dissociation into single cells via the sequential application of dispase (2 mg/mL; Thermo Fisher Scientific; 17105-04) and 0.05% trypsin (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... samples were incubated with primary Chr 2 intron probes for 30 hrs at 37°C in 50% Hybridization Buffer (2×SSC, 50% Formamide (v/v) (Thermo Fisher Scientific, Cat. AM9344), 10%(w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco], 2 mM L-glutamine, 0.1% of sodium bicarbonate [wt/vol; Gibco], 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... the corneas were cut into 2×2 mm pieces and sterilized by soaking in 1X Gibco phosphate buffered saline (PBS) (Fisher Scientific, 10-010-023) containing 1% HyClone penicillin streptomycin 100X solution (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... species-matched allogeneic T cells were added to passaged human organoids or passaged Balb/c small intestinal organoids with either human IL-2 (30 U/mL, Peprotec) and Dynabeads human T-activator CD3/CD28 (ThermoFisher, 2 µL per well), or murine IL-2 (30 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... 50 or 100 Multiplicity of Infection (MOIs) as described in the individual experiments in 2% complete medium (2%CM - DMEM F12 (Thermo Fisher, Waltham, MA, USA), 2% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then incubated in primary antibodies against VPRBP (1:80) and fibrillarin (1:100) for 2h at RT followed by Alexa flour 594 Goat anti mouse (Invitrogen, Cat # A11020) or Alexa flour 488 Goat Anti R (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated for 2h at RT in 1% BSA with the appropriate Alexa-Fluor secondary antibody (1:300; Life Technologies, USA) dissolved in 0.5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were trapped at 20 μL/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) in 1X blocking buffer for 45 min ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) for 45 min in 1X blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: We seeded 2×105 cells / well into 6 well dishes and replaced media with 5% KSR (Knockout Serum Replacement) (Gibco) containing media the next day ...
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bioRxiv - Cell Biology 2020Quote: ... collected into a new tube and stained with 4′,6-diamidino-2-phenylindole (DAPI) (∼5 µg/ml, Molecular Probes # D1306). Round coverslips (Ø12 mm ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 × 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Linear Trap Quadrupole Orbitrap Velos (with CID ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with PBS 0.1% Triton X-100 for 5 minutes and nuclei were counterstained with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS 0.1% Triton X-100 for 5 minutes at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... freshly cultured hESC colonies were dissociated into clumps (2-5 cells) with Versene buffer (with EDTA, Gibco, Cat. No. 15040066). The dissociated cells were seeded on Geltrex-coated 6-well tissue culture plates at 25,000 cells/cm2 (0.25M per well in the 6-well plates ...
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bioRxiv - Immunology 2022Quote: ... The cells were individually collected using an automated micro-manipulating system (CellCelector, ALS) and deposited in nuclease-free microtubes containing 5 μL of 2× CellsDirect buffer and RNase Inhibitor (Invitrogen). Single cell RT-qPCR was then performed using the protocol ADP41 developed by Fluidigm ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μm) and a C18 analytical column (PepMapAcclaim C18, 50 cm x 075 mm, 2 μm, Dionex-Thermo-Fisher Scientific), applying a linear gradient from 2 to 35 % solvent B (80 % acetonitrile ...
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bioRxiv - Genetics 2020Quote: ... and 2 μg of plasmid encoding tethered NHA-protein using 5 μl of Lipofectamine 2000 and OPTI-MEM media (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... neurons facing down, onto dishes containing the feeder layer of glia and 5 ml of culturing medium (94% Neurobasal, 2% Glutamax (Invitrogen), 2% B27 ...
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bioRxiv - Microbiology 2021Quote: Plasmodium falciparum blood stage parasites were maintained at 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 130 × g for 5 min and the pellet was resuspended in culture medium consisting of Neurobasal medium with 2% B27 (Invitrogen), 1% L-Glutamine (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at RT and were exposed to ECL imager after 5 min incubation with ECL reagent (Thermo Fisher).
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bioRxiv - Cancer Biology 2020Quote: Cells were sorted into 96-well plates at 50 cells/well in 5 µL CellsDirect™ 2× Reaction Buffer (Invitrogen). Cells lysates were reverse transcribed using Superscript™ III (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μm) and a C18 analytical column (PepMapAcclaim C18, 50 cm × 0.75 mm, 2 μm, both Dionex-Thermo Fisher Scientific), applying a linear gradient from 2 to 35% solvent B (80% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... 4390771 ID: s69588 and s69586) or ERK3 siRNA (5 pmol; catalog number: 4390771 ID: s78451) and Lipofectamine 2000 (2 µl) (Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated on an Acclaim PepMap 100 (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) trap column and separated on an Acclaim PepMap RSLC (75 μm × 50 cm ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were washed 2 × 5 min in PBS and mounted in ProLong® Gold antifade reagent (Life Technologies, P36934). Antibodies used for immunohistochemistry and their working concentrations are listed in Table 1.
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were permeabilised in 0.2% triton for 5 min and incubated with Click-it reaction cocktail (Click-it Alexa Fluor 647 C-10424 Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity 5 and mixed together with 2% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (A3678 ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 μm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Bioengineering 2022Quote: ... 721.221 cell lines were kept in a humidified incubator at 37°C with 5% CO2 and cultured in complete RPMI1640 medium supplemented with 2 mM GlutaMAX (Invitrogen), 10% FBS (Sigma),10 mM HEPES pH 8.0 (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The inoculum was removed and cells washed 2× with PBS and 500 μL culture media (high glucose DMEM + 5% FBS (Gibco) + 1% antibiotic-antimycotic ...