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Citations for Thermo Fisher :
8051 - 8100 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with secondary antibodies at room temperature for 2 h at 1:500 dilution: anti-rabbit AF594 (Invitrogen, A21207); anti-rat AF488 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 times with PBS and incubated with the following secondary antibodies at RT for 2 h: Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; Molecular Probes) and Alexa Fluor 633-conjugated goat anti-rabbit IgG (1:1,000 ...
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bioRxiv - Biophysics 2023Quote: Peptides were desalted for 4 minutes on a trap column (1 mM ID x 2 cm, IDEx C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific). Peptides were then separated on a C18 analytical column (Waters Acquity UPLC BEH C18 Column ...
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bioRxiv - Cell Biology 2023Quote: ... The vector and insert (1:2) were ligated using T4 ligase following the manufacturer’s instructions (Thermo Fisher Scientific Cat. No. EL0011). The cloned plasmid (here after denoted as pHBx ...
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bioRxiv - Biochemistry 2023Quote: ... Two datasets were collected with a total of 5988 movies (dataset 1) or 5050 movies (dataset 2) and were recorded automatically with EPU software (Thermo Scientific) at a total electron dose of 62.2 e/Å2 (dataset 1 ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 2 hours at 4°C in a 1:1000 solution of AF-568 donkey anti-mouse secondary antibody (Invitrogen #A10037) in blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... 5μg of the infectious clone and 100ng of a SARS-CoV-2 WA-1 nucleoprotein expression plasmid were diluted in 100μL of OptiMEM (Gibco, Waltham, MA). 3μL of TransIT-2020 (Mirus Bio ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of 1 mM ʟ-photo-leucine and 2 mM ʟ-photo-methionine (Thermo Fisher Scientific, Waltham, MA, USA). Incorporation of these photo-reactive amino acids (pAA ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were washed in 2 mL PBS 2x after which 200 µL of freshly made propidium iodide solution (.1% Triton-X 100 (Fisher Scientific), 50 µg/mL propidium iodide (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
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bioRxiv - Neuroscience 2023Quote: ... repair template (Supplemental Table 1) were introduced into ARPE-19 cells using electroporation (1400 V, 20 ms, 2 pulses, Neon Transfection System, Life Technologies). After electroporation ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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bioRxiv - Neuroscience 2023Quote: ... and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid) on an Acclaim PepMap100 RSLC C18 analytical column (2 μm, 75 μm id x 50 cm, [Thermo Scientific]) with a flow rate of 0.3 μl/min ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into Expi293F cells at a mass ratio of 2:1 (HC:LC) using ExpiFectamine 293 Reagent (Thermo Fisher Scientific). After transfection ...
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bioRxiv - Immunology 2023Quote: ... The heat-killed bacteria were stained 1:500 with Oregon Green 488-X succinimidyl ester (2 μL of dye from stock in 1 mL of PBS) (Thermo Fisher) fluorescent dye for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of eluted RNA was treated with 2 U of DNase using the Invitrogen Purelink DNase kit (ThermoFisher, Waltham, USA) and incubated at 37°C for 30 minutes followed by inactivation at 62°C for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: The tissues were stained with 2 μg ml-1 CellMask™ Deep Red plasma membrane stain (ThermoFisher Scientific, Waltham, MA, USA) for at least 24 h before imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were resuspended in 1mL of FACS buffer (PBS with 2% FBS and 1mM EDTA) containing a live/dead dye diluted 1:2000 (Live/Dead Fixable Aqua, Invitrogen #L34957) and incubated for 10 min at RT ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... The reaction was stopped by addition of 10 µL 10% TFA and samples were desalted by stagetipping with C18 filter plugs (Solaµ HRP 2 mg/1 ml 96 well plate, Thermo Scientific). Peptide samples were stored in 40 µL of 0.1% Formic acid at 4 °C until LC-MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS and medium was replaced by NM+VitA (DMEM/F-12, 1% N2-supplement, 2% B27+VitA supplement (Thermo Fisher), 1% P/S) ...
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bioRxiv - Neuroscience 2023Quote: ... The following day sections were washed in PBS and then incubated for 2 hours at RT in secondary antibody (Alexafluor 488 donkey anti-mouse at 1:200; Invitrogen A21202) following which they were washed ...
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bioRxiv - Physiology 2023Quote: ... followed by incubation for 2 h at room temperature with Alexa Fluor 680-linked anti-mouse IgG (A21058; Invitrogen, 1:5000). Signals were visualized with an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Immunology 2023Quote: ... were grown with 3T3 feeder layer support in a 2:1 mixture of Dulbecco’s Modified Eagle Medium (DMEM; Thermofisher; Cat # 12100061) and Ham’s F-12 nutrient mix media (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... and the EDL was immediately incubated in plating medium (DMEM + 10% horse serum + 2% Penicillin/Streptomycin + 1% HEPES) containing type I collagenase (2.6 mg/mL; Gibco, 17100-017) in a 37°C shaking water bath for 53 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 15 min in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI) solution (1:5000; catalog no. D1306, ThermoFisher Scientific, USA), followed by four 15-min washes in PBS with 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Biophysics 2024Quote: ... followed by a 2-minute incubation at 37 °C (with gentle rotation) in 1 mL warm TrypLE (Thermo Fisher Scientific, #12604013). The majority of TrypLE was removed by pipetting after incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA ID: s6696, s6697 and s6698 for HNF4A and cat# 4390843 and 4390846 for negative control #1 and #2, respectively, Thermo Fisher) and gene expression analysis was performed as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... pre-REP TIL were co-cultured with 2×107 40 Gy-irradiated allogeneic feeder PBMC (1:200 ratio) in upright 25-cm2 flask (ThermoFisher Scientific) in REP-TIL culture medium ...
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bioRxiv - Bioengineering 2024Quote: ... For other experiments, 2 ESC cell lines (H1 and H9, WiCell #WA01 and #WA09 respectively) and 1 episomal iPSC cell line (ThermoFisher, #A18945) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Click-it reaction was performed after staining with primary antibodies by incubating cells in Click-it buffer (100 mM Tris-HCl pH 8, 2 mM CuSO4, 1 ng Alexa Fluor 647 Azide (Life Technologies), and 100 mM ascorbic acid for 30 min at room temperature (RT) ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were co-transfected into Expi293F cells at a 2:1 (HC:LC) mass ratio using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol for a 25 mL culture ...
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bioRxiv - Physiology 2024Quote: Isolation of ADSC-derived small extracellular vesicles (sEVs) involved culturing ADSCs in high-glucose DMEM with 2% exosome-depleted FBS and 1% penicillin/streptomycin (Gibco, A2720803) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times for 15 min in PBS before a 2 h incubation with either a donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (dilution 1:500, Invitrogen, #A21208) or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500 ...
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bioRxiv - Microbiology 2024Quote: MDCK or MDCK SIAT1 cells were seeded in 96-well plates at 1-2 ξ 104 cells/well in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected with Glod4-targeting siRNAs (siRNA Glod4 #1: Cat. #43390816 ID s84641; siRNA Glod4 #2: Cat. #4390816 ID s84642, Thermo Scientific); siRNA scrambled (siRNAscr ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were washed with wash buffer (0.05% Tween 20 in PBS) and blocked with 2% BSA (Biowest) in PBS containing 1% Casein blocker (Thermo Fisher Scientific) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... and rinsed and then incubated for 2 h with appropriate Alexa Fluor-conjugated secondary antibodies (R37117, Thermofisher, Waltham, MA, 1 : 500). Fluorescent images were acquired using a Nikon A1R+ laser scanning confocal microscopy system on a Nikon Ti2-E inverted microscope ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Immunology 2024Quote: ... The epithelial solution was spun down at 1000 g for 2 minutes and then resuspended in 1 mL of TrypLE express enzyme (ThermoFisher #12604013) for 5 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... samples were incubated in Tyramide development solution (2% Dextran sulfate, 0.0015% hydrogen peroxide, 0.2mg/ml Iodophenol, 1:100 Alexa Fluor 594 Tyramide Reagent (Thermo Scientific, Cat. # B40957) in PTw ...
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bioRxiv - Molecular Biology 2024Quote: ... and their bladders were excised and placed into 2 mL conical-bottom screw-cap tubes (USA Scientific #1420-8700) on ice containing 1 mL TRIzol (Invitrogen #15596018) and six 2.8 mm diameter ceramic beads (Omni International #19-646) ...
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bioRxiv - Bioengineering 2024Quote: ... the embryos were incubated in secondary antibody solution (1 mL CAS-Block containing 2 μL Alexa Fluor 488 (Invitrogen, A-11006), 2 μL Alexa Fluor 594 (Invitrogen ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... A549 and/or H1299 cells were cotransfected with 2 ug of NPRL2 plasmid DNA (pKGB2-NPRL2) and 1 ug of pcDNA3.1 vector (Invitrogen, Carlsbad, CA) containing a neomycin-resistant gene ...
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bioRxiv - Immunology 2024Quote: ... as well as transmembrane serine protease 2 (TMPRSS2) were seeded at a density of 1 × 104 cells/well in white-walled 96-well plates (ThermoFisher Scientific) one day prior to usage (day 0) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were co-transfected at a 1:2 heavy to light chain ratio into Expi293F cells using the Expifectamine 293 Expression Kit (Thermo Fisher), and antibodies were purified with protein A agarose (Invitrogen) ...