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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... a 2 ml deep-well plate (1 ml of suspension per well, eight replicates per treatment) and a honeycomb plate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... were added to 1-2 mg of lysates for overnight incubation at 4 °C before the incubation with protein G Dynabeads (Life Technologies) for additional 2 hrs ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Physiology 2020Quote: ... cDNA (1 μl) was added to a reaction master mix (10 μl) containing 2× SYBR green qPCR reaction mix (Thermo Scientific) and gene-specific primers (5 μM of forward and reverse primers) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells seeded on 13 mm coverslips placed in 24 well plates were transfected twice (on day 1 and 2) with Oligofectamine or RNAi MAX (Thermo Fisher) complexed with 20pmol of each indicated siRNA and analyzed 3 to 4 days after the first transfection ...
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bioRxiv - Biophysics 2021Quote: ... qPCR was carried out in technical triplicates with 2 µl of 1:40-diluted cDNA per 10 µl reaction using SYBR Green PCR Mastermix (Applied Biosystems) with a final primer concentration of 500 nM ...
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bioRxiv - Immunology 2021Quote: ... ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher) was determined by AmplexRed assay [3] ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS then suspended in goat anti-mouse IgG (H&L) cross-adsorbed secondary antibody Alexa Fluor 488 (2 mg mL−1; Thermofisher Scientific) in PBS 0.1 % BSA for 45 min at 4 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: Sections were rinsed three times in PBST and incubated for 2 hrs at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Three washes in PBST were performed ...
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bioRxiv - Cancer Biology 2020Quote: ... PBMC were maintained in complete RPMI 1640 and treated directly with MEDS433 alone or with Dypiridamole (0.1–1 μM) or stimulated with 2% phytohemagglutinin (PHA, Gibco, Thermo Fisher Scientific), IL2 200U/ml (Peprotech ...
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bioRxiv - Bioengineering 2020Quote: ... the media was removed and the gels were washed once with PBS prior to the addition of 500uL of 1X PBS with Calcein AM (2 µM) and Ethidium Homodimer-1(4 µM) (Invitrogen, L3224). After 45 minutes of incubating the stains ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Neuroscience 2021Quote: ... Washed cells were incubated with PBS2+ containing 2 mg mL−1 Pierce Premium Grade Sulfo-NHS-SS-Biotin (PG82077; Thermo Scientific) for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections containing infarct core and penumbra were blocked with 5% BSA in TBS with 0.5% v/v Triton X-100 (TBS-T) and incubated with anti-MAP-2 (1:200; Invitrogen, PAS-17646) and anti-TUJ-1 antibodies (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Three hours after plating medium was exchanged for maintenance medium (Neurobasal-A without Phenol Red, 2% B-27 supplement, 1% penicillin-streptomycin, 0.5 mM glutaMAX, 25 μM β-mercaptoethanol; ThermoFisher Scientific) and cells were kept at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 h at RT in the secondary antibody (1:1000, goat anti mouse Alexa Fluor 647, Life Technologies) carrier solution (1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in 3×10 min in 0.1% PBT and kept in the same solution in the fridge until mounted (1 or 2 days later) and coverslipped with ProLong Diamond with DAPI (Thermo Fisher). Cells were imaged using a confocal microscope (Nikon A1si ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in 3×10 min in 0.1% PBT and kept in the same solution in the fridge until mounted (1 or 2 days later) and coverslipped with ProLong Diamond with DAPI (Thermo Fisher).
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bioRxiv - Cancer Biology 2021Quote: ... the medium was replaced with 1 mL of fresh medium with 2 drops of NucBlue LiveProbe reagent (Thermo Fisher; Cat#: R37605). Live imaging was performed on a Leica DMI8 microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-2 μg of total RNA was used to generate cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Gene expression in mouse cells was quantified using specific primers from Life technologies for mGAPDH (Mm99999915_g1) ...
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bioRxiv - Biophysics 2021Quote: α2-GFP or α2:β at 1:3 ratio was transfected into HEK293T cells on collagen coated glass bottom dishes using lipofectamine 3000 (Invitrogen) for expression of α2 GlyR or α2β GlyR ...
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bioRxiv - Cancer Biology 2020Quote: ... The sections were then incubated with Alexa Fluor 488 antimouse and the Alexa Fluor 546 F(ab’)2 fragment of goat antirabbit IgG (HþL) (Invitrogen; 1:2,000) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... GST-COSA-1 and CDK-2-6xHis were co-expressed and purified from Sf9 cells using the Bac-to-Bac system (Life Technologies). Infected High-Five cells (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... About 1-2 μg total RNA was reverse-transcribed into cDNA using the SuperScript™ III First-Strand Synthesis System (Invitrogen). Qualitative PCR was performed with GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
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bioRxiv - Microbiology 2020Quote: ... 10 μl of the resulting 1:10 dilution were mixed with 10 μl of a 1:100 dilution of the ERCC spike-in mix 2 (Thermo Fisher) and subjected to library preparation for next-generation sequencing (vertis Biotechnologie) ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA levels were quantified by quantitative reverse-transcriptase PCR (qRT-PCR) specific for the SARS-CoV-2 E gene (23) using TaqMan Fast 1-Step Master Mix (Applied Biosystems) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... The dengue 2 E protein antibody PA5-32246 was used at 1:10,000 for western blotting (Thermo-Fisher Scientific, Waltham, MA). Secondary detection for western blotting used anti-rabbit HRP antibody diluted 1:10,000 (Amersham ECL ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Microbiology 2020Quote: ... The buffer with dissolved gel slice was added to 1 mL ice-cold 100% ethanol and 2 μL of glycogen (20 mg/ml, ThermoFisher, R0561) and incubated at −20°C for at least one hour ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using ketamine / xylazine and intraviteally injected with 2 μl AF488 cholera toxin subunit B (1 mg/ml in PBS) (ThermoFisher Scientific). After 72 h mice were anaesthetized and euthanized via 4% PFA cardiac perfusion ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed three times in PBS – 0.05% saponin and incubated for 2 h with a donkey anti-goat Alexa Fluor® 488 (1/1000, Invitrogen A11055) diluted in PBS – 0.05% saponin – 0.2% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were left at room temperature for 24 hours and run in a 2% agarose gel containing 1/10000 SYBR Gold (ThermoFisher S11494) at 90V for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... ticks from each pool were put into a 2 mL centrifuge tube containing 1 mL of Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) prior to being homogenized in a frozen grinder (Jingxin ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in water at a desired concentration of 1-2 ug/uL as measured by a Qubit 3.0 dsDNA assay (Thermo Fisher Scientific). This product was subsequently used as the template for a dsRNA synthesis reaction (MegaScript RNAi ...
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bioRxiv - Cancer Biology 2020Quote: ... Harvested cells were spinned at 400g for 5 min and pellet was suspended at 1×106 cells per ml in pre-warmed DMEM (supplemented with 2% serum) media (Gibco, Invitrogen) in two tubes ...
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bioRxiv - Genomics 2021Quote: ... Cells were then quickly washed with 2 ml of PBS followed by permeabilization with 1 ml of PBS with 0.1% Triton X-100 (Thermo Fisher, 28314) for 10 min at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... At the end the cells were resuspended in 2 ml of medium (DMEM GlutaMax supplemented by 10 % FBS and 1 % antibody from Thermo Fisher Scientific, Invitrogen), counted via the trypan blue method and put in T-75 flask with approximately 20 ml of medium ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated with the indicated primary antibodies in PBS 2% FBS buffer overnight following by 45 min incubation with secondary antibodies and 1 μg/ml Hoechst (ThermoFisher, H1399).
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated 2 hours at room temperature with a secondary antibody donkey anti-rabbit Alexa 555 (1:500 in PBST, ThermoFisher, A31570). After Hoechst counterstaining ...
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bioRxiv - Cell Biology 2022Quote: The fractionated peptides were resuspended in 1% (v/v) TFA + 2% ACN and roughly 1.5 μg were injected onto nanoHPLC Dionex UltiMate 3000RS (Thermo Fisher Scientific) in combination with Thermo Orbitrap Fusion ...
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bioRxiv - Cell Biology 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit, Invitrogen, 32260; anti- mouse, Invitrogen, 32230). Coverslips were washed 3 times with blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 1×106 Cmtr1KD cells were transfected with 2 μg pPyPCAGGS vector (empty-vector or HA-CMTR1) using Lipofectamine 2000 (Life Technologies). Successfully transfected cells were selected with puromycin (1 μg/mL).
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was stained in 20 ml of 1 x TBE buffer containing 2 μl of SYBR Gold stain (ThermoFisher Scientific) for 5 minutes at room temperature and a band migrating just above the 35 nt oligo was cut from the Ribo-seq sample lane.
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bioRxiv - Molecular Biology 2022Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 μm ID x 15 cm, 2 μm, Thermo Scientific) mounted on a DPV ion source (New Objective ...