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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... siHIBCH (SASI_Hs01_00064760) or siMCCC2 (SASI_Hs01_00039625) was transfected into cells with RNAiMAX transfection reagent (ThermoFisher, #13778150) diluted 1:25 in serum free RPMI 1640 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (Gibco, Carlsbad, CA, USA), 2 mM glutamine (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10X Bolt™ Sample Reducing Agent (ThermoFisher, #B0009) and loaded onto a Bolt™ 4-12% Bis-Tris Mini Protein Gel (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were seeded at a density of 10,000 cells per well in standard phenol red-free RPMI 1640 (ThermoFisher, #11835055) with 5% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mM sodium pyruvate (ThermoFisher, #11360070) adjusted to pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... The passaging of cells was carried out at a confluence of 70– 80% with trypsin-EDTA (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... 10 mg/mL penicillin (Gibco, Carlsbad, CA, USA), 10 mg/mL streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then diluted (1:5) with RNAase/DNAase-free water and qRT-PCR performed using SYBR-Green Master Mix (ThermoFisher, #4309155) and primers listed in Supplementary Data ...
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bioRxiv - Cell Biology 2024Quote: ... (Invitrogen) solution was added to the pre-gel mixture (1.8 v/v%) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-RAD51 (Invitrogen, MA1-23271), anti-RNF8 (14112-1-AP ...
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bioRxiv - Developmental Biology 2024Quote: ... Worms were incubated in 10µM OPP (Invitrogen) diluted with M9 buffer containing 2×108 CFU/mL of dead OP50 in a total volume of 1 mL for 3 hours at 20 °C with gentle shaking ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 10% Fetal Bovine Serum (FBS) (Catalog No. 25-514H, Genclone, USA) and 1% Penicillin-Streptomycin (Catalog No. 15140148, Gibco, USA). For transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and either 2.00 mM or 0.15 mM glutamine (Life Technologies). Cells grew to approximately 90% confluence by the end of the collection period ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were selected in puromycin (Gibco; A1113803) (1.2mg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was isolated using a midi-prep kit (Invitrogen; K210005). The integrity of the CRISPRi library was validated by Illumina Miseq through Cornell Genomics Facility (detailed library preparation steps are presented below in “Library validation and CRISPRi dropout screening”).
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bioRxiv - Cell Biology 2024Quote: ... These flies were submerged in ice-cold 1X PBS (Thermo Fisher Scientific) during the micro-dissection procedure ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco; 15140122), and 0.18% β-mercaptoethanol (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The glands were then incubated with 500 nM Lysotracker Red (Thermo Fisher Scientific) for 5 minutes on ice ...
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bioRxiv - Cell Biology 2024Quote: ... This involved culture for 24 h in serum-free DMEM/F12 medium without L-glutamine (#21331046; Life Technologies), which was supplemented with 1% ITS (Insulin-Transferrin-Selenium ...
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bioRxiv - Cell Biology 2024Quote: ... (Thermo Fisher Scientific) for 48 h at 4 °C on a vertical rotator at 20 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... Si-RNA transfections were carried out using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: Total RNA from cells and tumor tissues was extracted using Trizol (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... in 50 μL Opt-MEM (Thermo Fisher Scientific). Media was changed 16 hours later to 1.5 mL fresh complete media ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were identified in Proteome Discoverer 2.4 (Thermo Fisher Scientific) using Sequest HT ...
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bioRxiv - Cell Biology 2024Quote: The sites of unsaturation in glycerophospholipids were elucidated using a high-resolution mass spectrometer (Orbitrap Elite, Thermo Scientific), modified to allow for ozone-induced dissociation (OzID ...
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bioRxiv - Cell Biology 2024Quote: ... 75 μm ID EasySpray column (ES803; Thermo Fisher) on a 60-minute gradient of 2 to 35% acetonitrile (containing 0.1% formic acid and 5% DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... The organic fraction was evaporated using a Savant Speedvac spd111v (Thermo Fisher Scientific) at room temperature and the remaining lipid pellet was stored at -20°C under argon.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3 times in wash buffer and incubated in CY5-conjugated antibodies at 1:300 and 0.6 mg/mL Hoescht-33342 (Invitrogen) for one hour with rocking at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected into fluorescence-activated sorting tubes and co-stained with 5 µM 7AAD (Invitrogen) and 1X BD Pharmingen™ PE Annexin V (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... and scraped with 300 µL extraction buffer 1:1 LC-MS methanol:water (Optima®, Thermo Fisher Scientific) into a 1.5 mL microcentrifuge tube ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with Dulbecco’s phosphate-buffered saline (DPBS) 1X (Biowest) and detached enzymatically-TrypLE (Thermo Fisher). Cell suspension was collected to 15mL centrifuge tubes ...
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bioRxiv - Cancer Biology 2024Quote: Cells were washed with ice-cold PBS three times and treated with 1-5mM disuccinimidyl suberate (DSS, A39267, Thermo Scientific, Waltham, MA) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were cultured in the presence of 2.5 µM BrdU for one population doubling before 0.2 µg/ml colcemid (Gibco 15212012) treatment for the final 4 hours before trypsinization ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cells grown on 13 mm circular glass coverslips (Thermo Fisher Scientific) were pre-extracted in 0.2% Triton x-100 in PBS for 2 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Both types of media were supplemented with 10% FBS (Gibco) and maintained in a humidified atmosphere at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1mL of highly concentrated bacterial culture was seeded into 9mL of Middlebrook 7H9 + 10% ADC + Hygromycin 50μg/mL in 30mL sterile bottles (Cat. No. 2019-0030 – Thermo Scientific) and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... whereas siRNA was delivered using Lipofectamine™ RNAiMAX (ThermoFisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... and Bcl-xS antibody was from ThermoFisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2024Quote: ... including IMDM (Thermo Fisher, No.12440053) and Ham’s F12 (Corning ...
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bioRxiv - Developmental Biology 2024Quote: ... and goat anti-mouse IgG (1:10000, Invitrogen; 31430) and goat anti-rabbit IgG (1:2000 ...
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bioRxiv - Developmental Biology 2024Quote: Each microdissected tissue was singularized with TrypLE Express (Life Technologies, Cat# 12604-013) at 37C and quenched with 1% FBS in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were then washed in media containing 10% fetal bovine serum (FBS, ThermoFisher), centrifuged at 300 g for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNAs from cells carrying CRISPRi sgRNA library were extracted using a Genomic DNA Purification Kit (Thermo Scientific; K0722). To recover sgRNA sequences ...
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bioRxiv - Developmental Biology 2024Quote: ... Glutamax (ThermoFisher, No. 35050-061), ascorbic acid (Sigma,A4544) ...
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bioRxiv - Developmental Biology 2024Quote: ... washed in PBS containing 10% fetal bovine serum (FBS, ThermoFisher), centrifuged at 500 g for five minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... Three micrograms of RNA were reverse transcribed into cDNA using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA), according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from cells using TRizol Reagent (Invitrogen; 15596018). Purified RNAs were used for Illumina RNA-seq library preparation with NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... with B27 Supplement with retinoic acid (Gibco, No.17504044), N2 Supplement (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplifications were performed on an ABI PRISM 7000 thermal cycler (Applied Biosystems) using the following cycling conditions ...
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bioRxiv - Developmental Biology 2024Quote: Cells were lysed and homogenized using RIPA buffer (Thermofisher; 89900) with protease inhibitors (Sigma ...