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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Approximately 800,000 U2OS cells were reverse transfected with 20 nM siRNA against the 3’ UTR of endogenous TIRR or a negative control siRNA (Dharmacon ON-TARGETplus Human NUDT16L1 siRNA J-014872-23-0005 or ON-TARGETplus Non-targeting Control) using 3 uL of RNAiMAX lipofectamine (Thermo Fisher) in a 6-well plate ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were then transfected with 25 nM of either ON-TARGETplus Human ABCB1 (5243) SMARTpool or non-targeting pool siRNAs (negative control, Dharmacon) using the Lipofectamine RNAiMAX reagents (Thermo Fisher). Following 24 hours of incubation ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies or co-stains were as follows: Alexa Fluor 647-conjugated streptavidin (surface display staining other than TfR human/mouse cross-reactivity, ThermoFisher S21374); iFluor 647-conjugated anti-His-tag antibody (pilot CYpHER detection and TfR human/mouse cross-reactivity ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Cell Biology 2024Quote: ... human EGF (10 µg/ml) and bovine insulin (10 µg/ml) in low-attachment plates (Nunclon sphera, Thermo Scientific™ #174932) and maintained at 37°C in a humidified 5% CO2 incubator for 3 days before harvesting ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Pathology 2024Quote: ... and slides were analyzed independently by 3 experienced reviewers to identify and measure the area of human cardiomyocyte grafts using an EVOS (Life Technologies) microscope ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Microbiology 2024Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... University of Belgrade-Faculty of Biology which was previously produced by several reactions of cloning and subcloning of CTG repeats originating from human DMPK locus using pJet1.2/blunt cloning vector from CloneJET PCR Cloning Kit (Thermo Fisher Scientific). The subcloned region sequence was verified with Sanger sequencing before prior use ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 million SH-SY5Y cells stably overexpressing human A53T mutated α-synuclein (Danzer et al., 2007) were resuspended in electroporation buffer R (NEON electroporation system, ThermoFisher) and added to the antibody-fibril preparation (final volume 100 μl) ...
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bioRxiv - Cancer Biology 2022Quote: 10 ng of total RNA was reverse transcribed with MegaplexTM miRNA-specific stem-loop RT Primers Human Pool A v 2.1 (Thermo Fisher Scientific) and TaqMan® MicroRNA reverse transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D7 blood-stage cultures were maintained at 4% hematocrit in human A+ erythrocytes in Rosewell Park Memorial Institute (RPMI) 1640/Hepes medium (Gibco, 11875119) supplemented with 0.5% Albumax II (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... a human brain organoid cultured during 4 months has been dissociated in TRIzol RNA isolation reagent (Thermo Fisher Scientific ref: 15596026). Extracted RNA has been processed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Microbiology 2023Quote: ... The experiments were normalized using human β-Actin as the endogenous control (FAM/MGB probe, cat. no. 401846; Applied Biosystems, USA).
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The following secondary antibodies and dilutions were used: Alexa Fluor 568- and 647-conjugated goat anti-human and anti-rabbit (Life Technologies), 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: The C28/I2 human chondrocytes were cultured in T-75 flasks with Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen Corp., Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Cancer Biology 2023Quote: ... human NRP-1 (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 using an Ab labeling kit (Molecular Probes, A20186). Images were acquired with an LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... (74) were cultured in keratinocyte serum-free media (KSFM) supplemented with bovine pituitary extract and human epidermal growth factor (Thermo Fisher). HCT116 human colon carcinoma lines were obtained from ATCC and maintained following their recommendations ...
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bioRxiv - Genetics 2023Quote: RT-qPCR experiments were performed as previously described (Coutton et al., 2019) with cDNAs extracted from various human tissues purchased from Life Technologies® ...
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bioRxiv - Bioengineering 2023Quote: The microdevices containing normal human lung fibroblasts with or without macrophages were fixed using a solution of 2% glutaraldehyde (ACROS organics) in PBS (containing Ca2+/Mg2+ ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Immunology 2023Quote: ... or with rabbit-anti-human TG3 (PA596450, Thermo Fischer Scientific) followed by detection with Alexa 488 donkey anti-rabbit (Molecular Probes) antibody ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Biochemistry 2023Quote: ... harboring a human APP transgene with the K670N and M671L Swedish mutations (25) were grown in DMEM/F12 medium (Thermo Scientific) supplemented with 5% FBS ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Bioengineering 2023Quote: ... wells were washed 3 times with PBS with 0.1% tween (PBST) and then incubated overnight at 4 °C with a human hemoglobin-FITC conjugated antibody (Life Technologies, A80134F). Samples were washed three times with PBST prior to imaging on a Keyence BZ-X800 microscope.
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bioRxiv - Cell Biology 2023Quote: ... expressing GFP-LC3A (human), were prepared as described previously (Fletcher et al., 2018; Jacquin et al., 2017) and cultured in DMEM/F12 (Gibco, 11320074) containing 5% horse serum (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Neuroscience 2022Quote: ... Preparation of nuclear and cytoplasmic extracts from human fibroblasts was performed using a NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher), as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Previously homogenized human-derived AD crude brain extracts were diluted 1 to 20 with Opti-MEM (Thermo Fisher Scientific, cat. 31985062) and sonicated in a Qsonica multiplate horn water bath for 3 minutes at 40% power ...
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bioRxiv - Immunology 2023Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... human prostate tissues were first digested in aDMEM/F12/Collagenase II (1.5mg/ml)/Hyaluronidase VIII (1000 u/ml; Thermo Fisher Scientific) plus 10 μM Y-27632 (Tocris ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Human liver homogenate was prepared from human FLC or matched normal liver tissue (adjacent to the FLC) using RIPA Lysis buffer (Thermo Fisher) with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... 25 nM) or Human YAP/TAZ-siRNA (UGUGGAUGAGAUGGAUACA) (102) by using Lipofectamine RNAiMAX reagent (13778075, Thermo Fisher Scientific, Waltham, MA, USA). Cells were harvested after 72 h.