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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1% w/v BSA) were precomplexed with anti-human Fc specific antibodies-HRP (A18817, ThermoFisher Scientific, 2:1 ratio lectin:antibody) and pre-incubated with 20 mM EDTA or 40 mM glucose ...
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bioRxiv - Cancer Biology 2024Quote: The fusion (no stop) human ID1 or Clover open reading frames were cloned into the pcDNA3.2 V5 destination vector (Invitrogen, 12489019) to create V5 tagged ID1 or Clover constructs ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse serum and purified human IgG Fc (#0855911) were diluted in LDS sample buffer (#B0007, Life Technologies, Carlsbad, California, USA) with 2.5% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293AD cells were stably transfected with pLVX-IRES-Neo encoding full-length human EphB4 with an N-terminal FLAG tag using Lipofectamine 2000 reagent according to the manufacturer’s instruction (#11668019, ThermoFisher Scientific). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Human fibroblasts were cultured on 100 mm Petri dishes (Falcon) in High Glucose Dulbecco’s modified Eagle Medium (DMEM, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... TRAC knockouts were detected via staining with anti-human alpha/beta TCR FITC (Thermo Fisher Scientific, catalog #11-9955-42). Anti-CD19 CAR expression was detected using FITC-labelled human CD19 protein (Acro Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... SH3GL2) in pDONR201 (HSCD00000899, Harvard Institute of Proteomics) and human endophilin-A3 (EndoA2, SH3GL3, IMAGE5197246-AK68-m23) in pENTR11 (Invitrogen) were transferred into pCMV-DEST- EGFP using Gateway® recombination (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Fully-adhered human fibroblasts were washed in PBS and immediately lysed in the tissue culture dish with 1xNUPAGE LDS sample buffer (Invitrogen) supplemented with 50mM DL-Dithiothreitol (DTT) ...
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bioRxiv - Cell Biology 2024Quote: The analysis of classical tetraspanin markers on WJ-MSC273 and WJ-MSC/TERT273-derived EVs was carried out using exosome-human CD81 beads (Invitrogen) to capture EVs ...
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GRASP55 Safeguards Proper Lysosome Function by Controlling Sorting of Lysosomal Enzymes at the GolgibioRxiv - Cell Biology 2024Quote: ... total RNA was isolated from human WI-26 fibroblasts using a standard Trizol/chloroform-based extraction (15596018, Thermo Fisher Scientific), and converted to cDNA using Superscript II (#18064014 ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM N-acetyl-L-cysteine plus 100 IU/mL of IL-2 and activated with Dynabeads Human T-Activator CD3/CD28 (Gibco) at a 1:1 bead-to-cell ratio.
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bioRxiv - Genetics 2024Quote: ... C247R we obtained constructs which were codon-optimized for Drosophila and encoded the human protein from GeneARTTM (Thermo Fisher Scientific) (See Supplemental Text) ...
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bioRxiv - Genetics 2024Quote: ... 250.000 human or murine iPSCs were transfected with Lipofectamin RNAiMAX (Cat. No. 13778075) according to the manufacturers protocol (ThermoFisher Scientific). Ambion® Pre-miR™ miRNA Precursor for hsa-miR-197-3p (Assay ID PM10354 ...
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bioRxiv - Microbiology 2020Quote: 293T cells (ATCC) and 293FT cells (Invitrogen) were cultured in Dulbecco’s modified Eagle media (DMEM ...
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bioRxiv - Cell Biology 2022Quote: Mother cells were stained using CFSE (Cell Trace CFSE Cell Proliferation kit Thermofisher), 5×105 cells were placed in a 60mm plate in 5mL of culture medium mixed with 5µL of CFSE at 5 mM (final concentration 5µM ...
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA-TruB1 was generated by inserting the ORF of human TruB1 with a Flag sequence into pcDNA.3.1(+) (Thermo Fisher Scientific) at the Nhe1 and EcoR1 sites ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNAs (siRNAs) specifically targeting human CD44 (siRNA IDs s2681 and s2682) were obtained from Life Technologies (Carlsbad, CA, USA). DPSCs (1×105 per well ...
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bioRxiv - Cancer Biology 2020Quote: ... were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher). Libraries were then purified via solid phase reversible immobilization (SPRI ...
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bioRxiv - Developmental Biology 2020Quote: ... Relative mRNA levels of a panel of inflammation-associated genes were initially screened using a Human Immune Taqman PCR array (4418718, ThermoFisher Scientific) and Taqman Fast Advanced Master Mix ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA samples were used for global gene expression profiling on human Clariom S Assay microarrays (Thermo Fisher Scientific, Wilmington, DE, USA), which include 24351 genes ...
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bioRxiv - Immunology 2022Quote: ... Blots were washed 3x with PBS-T and incubated for 1 h at room temperature with appropriate secondary antibodies: mouse IgGk-BP-HRP (cat# sc-516102, SantaCruz) or goat anti-human-HRP (cat# 31410, Thermo Scientific), diluted 1:10,000 in 5% blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Expression levels of Kif11 and APP were then measured by multiplex qRT-PCR using mouse Kif11 and human APP primer probes (Applied Biosystems, Mm01204225_m1 ...
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bioRxiv - Neuroscience 2021Quote: ... of human iPSC-induced neurons were plated on completely dry PDL- (100 µg/ml) and laminin-(10 µg/ml; Thermo Fisher) coated 96-well or 6-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... 100 units penicillin and 100 μg streptomycin per ml) supplemented with 50 U/ml IL-2 and 1 × 106 CD3/CD28-coated Human T-Activator Dynabeads/ml (Life Technologies). The next day ...
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bioRxiv - Immunology 2021Quote: ... BV421 anti-human CD69 together with Live/Dear-near IR (1:1000 in PBS; LD-NIR; ThermoFisher Scientific; Cat no L10119) for exclusion of dead cells ...
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bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA, Thermo Fisher Scientific) sequences using RNAiMax lipofectamine transfection reagent (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: The spike protein gene from MERS-CoV strain Camel/Qatar/2/2014 (GenBank accession number KJ650098.1) was codon optimized for humans and synthesized by GeneArt (Thermo Fisher Scientific). The synthesized S gene was cloned into a transgene expression plasmid comprising a modified human cytomegalovirus immediate early promoter (CMV promoter ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Immunology 2021Quote: ... with unconjugated rabbit anti-human phosphorylated NF-κB p65 (Clone: Ser536 93H1) followed by goat anti-rabbit IgG (H+L) Alexa Fluor 488 (Thermo Fisher). For p38 ...
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bioRxiv - Immunology 2021Quote: ... and either left them untreated (hence naive) or activated them ex vivo with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific), wherein the bead-to-cell ratio was 1:1 ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Human Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The raw spectra were searched against the Human (Homo sapiens) reference proteome database (UNIPROT) using SEQUEST HT (Proteome Discoverer v1.4, Thermo Fisher Scientific) with a Full MS precursor mass tolerance of 20 ppm and MS2 peptide fragment mass tolerance of 0.5 Da ...
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bioRxiv - Bioengineering 2020Quote: Cytokine analysis was performed on supernatants derived from cultures given the indicated treatments using a human cytokine 10-plex panel (Thermo Scientific) per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... human muscle biopsies from healthy donors were minced and digested in 0.8% w/v collagenase I (Life Technologies; Carlsbad, CA, USA) in DMEM ...
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bioRxiv - Neuroscience 2021Quote: The medial and baso-lateral amygdaloid nuclei were dissected from the mesial superior temporal lobe for 0.75-1 cm thick coronal slabs of frozen human brains on dry ice using a stainless steel punch (8mm diameter Thermo Fisher Scientific Integra Miltex standard biopsy punch) ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-Rabbit IgG Alexa Fluor 647 and Goat anti-Human IgG Alexa Fluor 647 were used at 1ug/ml concentration in FACS buffer (ThermoFisher Scientific). For western blotting ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 2.5 μg of the in vitro synthesized RNA was used to transfect ∼6 ×105 BHK-hACE2-N cells stably expressing the SARS-CoV-2 N and the human ACE2 genes(Rihn et al., 2021) using the MessengerMax lipofection kit (Thermo Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral overexpression of PNUTS was achieved by cloning the full-length human cDNA of PNUTS into pLenti4/v5-DEST (Life technologies). The PP1 non-binding mutant PNUTS (W401A ...
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bioRxiv - Microbiology 2021Quote: ... sodium bicarbonate and either a) 10% heat-inactivated A+ human plasma (Interstate Blood Bank, Memphis, TN) or b) 1% (w/v) AlbuMAX II (Thermo Fisher) and 320 μM hypoxanthine (Sigma) ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... ACE2 expression was assessed by staining cells with polyclonal rabbit antibodies against human ACE2 (PAB886Hu01, Cloud-Clone Corp.) followed by secondary anti-rabbit antibodies conjugated with PE (1:250, # P-2771MP, ThermoFisher Scientific). Samples were analyzed on CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 live cells were incubated with the serum from a convalescent donor at the 1:100 dilution in PSB for 30 min followed by the incubation with secondary anti-human IgG antibodies conjugated with PE (1:250, # H10104, ThermoFisher Scientific) for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Cytokine and chemokine levels in mycobacterial-infected supernatants were determined using human cytokine and chemokine and growth factor 45-plex panel 1 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...