Labshake search
Citations for Thermo Fisher :
6901 - 6950 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
-
bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
-
bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
-
bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
-
bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
-
bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
-
bioRxiv - Cell Biology 2024Quote: Previously generated microarray data from microdissected human glomeruli sourced from individuals with kidney disease and healthy donors were used (Affymetrix HGU133Plus2.0 ...
-
bioRxiv - Physiology 2024Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
-
bioRxiv - Microbiology 2024Quote: ... or the type II ME49 strain Δku80/Δhxgprt background (Waldman et al., 2020) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% calf serum and 10 μg/mL gentamicin (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The iPSCs were resuspended in human embryonic stem media with 10 ng/mL of bFGF (beta fibroblast growth factor, Invitrogen) (HES-10 ...
-
bioRxiv - Cell Biology 2024Quote: ... the human embryonic kidney cell line HEK293T (purchased from ATCC) and the human hepatocellular carcinoma HepG2 (purchased from ATCC) were maintained in DMEM medium (Gibco) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... permeabilized and blocked in PBS w/ 0.1% Tx-100 3% BSA and IF stained with goat anti-human IgG AF488 1:500 (Invitrogen A11013), mouse anti-Tubulin β 3 (TUBB3)-AF647 1:300 (BioLegend 801210 ...
-
bioRxiv - Cell Biology 2024Quote: Human pancreatic islets were cultured in Connaught Medical Research Laboratories (CMRL) 1066 medium (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) containing 5.6 mM glucose and supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... The siRNA sequences targeting human TFEB (5□-GAA AGG AGA CGA AGG UUC AAC AUC A-3□) were purchased from Invitrogen. The siRNA sequences targeting human NPC1 (5□- CAA UUG UGA UAG CAA UAU UTT-3□ ...
-
bioRxiv - Immunology 2024Quote: ... and the cell pellet resuspended in 5 mL of RP10 medium (RPMI 1640 [Gibco] supplemented with 10% human AB serum [Gemini BioProducts], 2mM L-glutamine [Gibco] ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were stained for RSV using a human anti-RSV antibody (Beiresources, Mannasas VA) and an Alexa Fluro 555 secondary (Invitrogen), were re-fixed and imaged.
-
bioRxiv - Microbiology 2024Quote: ... immuno-staining with 5 µg/ml anti-spike monoclonal antibody mAb48 (gift of Hugo Mouquet, institute Pasteur) 63 and alexa-Fluor-488-conjugated anti-human IgG antibody (Invitrogen) was performed at 4°C ...
-
bioRxiv - Microbiology 2024Quote: Plasmodium falciparum parasites were grown in human erythrocytes obtained from the Stanford Blood Center at 2% hematocrit in RPMI 1640 medium (Gibco). Medium was supplemented with 0.25% Albumax II (Gibco) ...
-
bioRxiv - Microbiology 2024Quote: ... cells were incubated for 1 hour with secondary antibodies (1:500 goat anti-human Alexa-fluor 647 [Invitrogen #A48279TR], 1:500 goat anti-rabbit Alexa-fluor 594 [Invitrogen #A32740] ...
-
bioRxiv - Immunology 2024Quote: YTHDF2 and CD8 in mouse samples or human specimen were detected by Tyramide SuperBoost kits (Alexa Fluor 488-labeled tyramide, Cy3-labeled tyramide) (ThermoFisher), according to the manufacturers’ protocol ...
-
bioRxiv - Immunology 2024Quote: ... and bound CLR-Fc’s were detected with goat anti-human IgG Fc Alexa Fluor 488 (1:200, Thermo Fisher Scientific) for Dectin-1-Fc ...
-
bioRxiv - Cell Biology 2024Quote: ... a codon optimized insert encoding for full-length human ABHD2 was synthesized at Thermofisher Scientific and cloned via Gateway technology (Invitrogen) into pD-INS26 ...
-
bioRxiv - Immunology 2024Quote: ... Plates were then washed thrice and incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Thermo Fisher Scientific) at 1:5,000 dilution for 1 hour at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... and the cell pellet resuspended in 5 mL of RP10 medium (RPMI 1640 [Gibco] supplemented with 10% human AB serum [Gemini BioProducts], 2mM L-glutamine [Gibco], and 100 U/mL penicillin/streptomycin [Gibco]). Cells were counted as described above ...
-
bioRxiv - Immunology 2024Quote: ... The plates were washed as before and incubated for 30 minutes at room temperature with anti-human IgG secondary antibody (Invitrogen) at a 1:10,000 dilution in 1% milk in PBS-T ...
-
bioRxiv - Immunology 2024Quote: ... and functional grade anti-CD3 (aCD3, 1 μg/mL) and anti-CD28 (aCD28, 1 μg/mL) murine or human monoclonal antibodies (Invitrogen)) ...
-
bioRxiv - Immunology 2024Quote: ... and functional grade anti-CD3 (aCD3, 1 μg/mL) and anti-CD28 (aCD28, 1 μg/mL) murine or human monoclonal antibodies (Invitrogen). Subsequently ...
-
bioRxiv - Immunology 2024Quote: ... were incubated with each indicated peptide (100 μM) and human β2-microglobulin (β2m, Prospec-Tany, USA, 2.5μg/ml) in serum-free Opti-MEM (Thermo Fisher, USA) for 16-18h at 26°C with 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... PE anti-human PD1 (clone MIH4, #12-9969-42) and PE-anti TOX (clone TXRX10, #12-6502-82) were purchased from Invitrogen. Pembrolizumab (#A2005 ...
-
bioRxiv - Immunology 2024Quote: ... the cells were stained with fluorescently labelled secondary antibodies (goat anti-human IgG Dylight488 from Invitrogen; catalogue number SA5-10134] and goat anti-mouse AlexaFluor647 from Invitrogen; catalogue number A32728] ...
-
bioRxiv - Microbiology 2024Quote: ... All secondary antibodies goat anti-(human, mouse, rat, or rabbit) (H+L) used for immunofluorescence (1:1000) were purchased from Invitrogen. Secondary antibodies for western blotting ...
-
bioRxiv - Cell Biology 2024Quote: ... Transcriptome analysis was done using the GeneChip Human Transcriptome Array 2.0 for in combination with the GeneChip WT PLUS Reagent kit (Applied Biosystems). 33,3ng total RNA was used as input for the analysis that was done according to the manufacturer’s guidelines ...
-
bioRxiv - Immunology 2024Quote: ... ELISAs for total IgM or IgG were also performed in parallel using human uncoated IgM or IgG ELISA kits (Invitrogen), following manufacturer’s instructions.
-
bioRxiv - Biophysics 2024Quote: ... Secondary antibodies include donkey anti-human IgG Alexa Fluor 555 (1:600) and Goat anti-mouse IgG Alexa Fluor 405 (1:500, Invitrogen). Confocal microscopy was performed using a laser scanning confocal microscope (LSM 880 ...
-
bioRxiv - Biochemistry 2024Quote: The purified full-length human and mouse CNPase (hCNPase and mCNPase) were immobilised overnight at +4 °C on a 96-well immunosorbent plate (Nunc). All the following steps were done by gentle shaking on an orbital shaker ...
-
bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes from PBMC buffy coats were isolated with anti-human CD14 magnetic beads (Miltenyi) and cultured in RPMI containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 50 U/ml penicillin ...
-
bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
-
bioRxiv - Immunology 2024Quote: ... chronic infected patient and HD groups by Luminex using the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (#EPX450-12171-901 ThermoFisher) according supplier’s recommendations ...
-
bioRxiv - Immunology 2024Quote: ... 1×106 cells were stained with the panel of antibodies in 100 µl of staining buffer containing 0.5 % human serum albumin (HSA, Albunorm, Octapharma) and 2 mM EDTA (ThermoFisher Scientific) in PBS (Invitrogen) ...
-
bioRxiv - Microbiology 2024Quote: Immortalized human HaCaT keratinocytes [46] and murine K38 keratinocytes [59] were cultured in high glucose DMEM media (GIBCO 11965-092) supplied with penicillin and streptomycin (GIBCO 15140-122 ...
-
bioRxiv - Cell Biology 2024Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: The 3xHA-NTSR1 plasmid consists of N-terminal 3xHA-tagged wild-type human NTSR1 cloned into the pcDNA3.1(+) vector (Invitrogen, Thermo Fisher Scientific Corporation ...
-
bioRxiv - Neuroscience 2024Quote: Duplex of the MEF2C enhancer sequence containing a mutation site [5′-ATGTATTTTTCTGCAATAAGT-3′ (×2)] in human genomic DNA were synthesized (Invitrogen). Additionally ...
-
bioRxiv - Molecular Biology 2024Quote: To generate PUF tethered constructs for full length RBPs and C-terminal/N- terminal fragments (i.e., effector domains), we used primers containing suitable restriction sites (XbaI, NotI) and human cDNAs for amplification (SuperScript III, Invitrogen). The amplified DNA fragments were then cloned into pGL-PUF backbone ...
-
bioRxiv - Physiology 2024Quote: ... TN where microarray gene expression profiling was performed with the Affymetrix Human Gene 2.0 ST expression array (Thermo Fisher Scientific). Gene expression analysis was performed in RStudio v2024.04.0 ...
-
bioRxiv - Immunology 2024Quote: ... Serum was serially diluted in media supplemented with 10% normal human serum and incubated with pseudotyped virus and dextran (ThermoFisher) for 1 hour at 37°C ...
-
bioRxiv - Physiology 2024Quote: ... hiPSC-aCM lines were maintained on human Biolaminin 521 LN-coated dishes in TeSR-E8™ medium (Thermo Fisher Scientific). Cardiac differentiation was carried out by monolayer culture on Matrigel® hESC-qualified Matrix (Corning ...
-
bioRxiv - Biochemistry 2024Quote: ... were maintained by continuous culture at 2-5% hematocrit in human erythrocytes with malaria culture medium (RPMI 1640 supplemented with 5 g/L Albumax II (Gibco), 0.12 mM hypoxanthine (1.2 ml 0.1 M hypoxanthine in 1 M NaOH) ...
-
bioRxiv - Cancer Biology 2024Quote: ... which can be accessed from www.cancerrxgene.org/.44 The expression data was collected using the Human Genome U219 96-Array Plate with the Gene Titan MC instrument (Affymetrix). This data was normalized using the robust multi-array analysis (RMA ...
-
bioRxiv - Microbiology 2024Quote: ... 1% w/v BSA) were precomplexed with anti-human Fc specific antibodies-HRP (A18817, ThermoFisher Scientific, 2:1 ratio lectin:antibody) and pre-incubated with 20 mM EDTA or 40 mM glucose ...