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Citations for Thermo Fisher :
6751 - 6800 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation (IP) of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human endogenous PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The low and high molecular weight membranes proteins were incubated with respective primary antibodies (Rabbit anti-human BAIAP2L1, PA5-54000, Invitrogen) or (Goat anti-mouse GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells or MiniBAR-GFP stable cell lines were transfected with 25 nM of siRNAs targeting either luciferase or human Mini-BAR (5’-CUGCAAAUUUUACGGAUCA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The wells were washed 5x and incubated with 100 µl/well with an HRP-conjugated anti-human IgG Fc antibody (1:10000 dilution, Invitrogen) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Human TSC media (TSCm) used herein was previously described in 70 with slight modifications: 470 ml DMEM/F12 (Invitrogen 21331), 5 ml Commercial N2 supplement (Invitrogen 17502048) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Genomics 2023Quote: ... These mRNAs were generated from cloned human cDNAs inserted into pCS2+ and in vitro transcribed using mMessage mMachine SP6 kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Transcriptome libraries were generated using automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The transcriptome libraries were barcoded ...
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bioRxiv - Cell Biology 2023Quote: NK cells were transfected with scrambled or ON-TARGET plus human WWTR1 SMARTpool siRNA from Dharmacon Inc, (CCGCAGGGCUCAUGAGUAU, GGACAAACACCCAUGAACA, AGGAACAAACGUUGACUUA, CCAAAUCUCGUGAUGAAUC) using the Neon Transfection System (Invitrogen) and according to the manufacturer recommended parameters ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for 2 hr at room temperature with the following antibodies at the manufacturer-recommended dilutions in 1× TFP Perm/Wash buffer: APC- eFluor780 anti-human CD3 (clone UCHT1, Invitrogen); PerCP-Cy5.5 anti-human CD4 (clone SK3 ...
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bioRxiv - Developmental Biology 2023Quote: ... floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher, Cat#12634010), 2 mM Glutamax (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The brain sections were then incubated with goat anti-mouse Alexa Fluor 488 and goat anti-human Alexa Fluor 647 (1:500, Invitrogen). Following secondary staining ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice with PBS and 100 μL of Goat anti-human IgG (H+L) Alexa647 secondary antibody (2 μg/mL, Invitrogen) and Aqua viability dye was added for 20 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... hTDP-43 mRNA was measured using the PrimeTime Gene Expression Master Mix (IDT) with the following TaqMan probes: human TDP-43 (Hs00606522_m1; ThermoFisher Scientific) and mouse Actb (Mm02619580_g1 ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were transfected with 50nm ON-TARGETplus Human SMARCA4 siRNA (Dharmacon L-010431-00-0005) or the corresponding siControl (D0012061305) by lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... MSC-EV preparation vesicular structure and canonical surface marker expression were determined using EV bead-based flow cytometry with human CD81 detection beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: We used the cytokine/chemokine/growth factor 45-Plex Human ProcartaPlex™ Panel 1 (Cat# EPX450-12171-901, ThermoFisher, USA) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Lipofectamine™ 3000 transfection reagent (InvitrogenTM, Cat# L3000001) and human PAR-1 si-RNA (CGGUCUGUUAUGUGUCUAUdTdT) were purchased from Invitrogen (USA) and Eurogentec (Belgium ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
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bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Cell Biology 2024Quote: Previously generated microarray data from microdissected human glomeruli sourced from individuals with kidney disease and healthy donors were used (Affymetrix HGU133Plus2.0 ...
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bioRxiv - Physiology 2024Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
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bioRxiv - Microbiology 2024Quote: ... or the type II ME49 strain Δku80/Δhxgprt background (Waldman et al., 2020) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% calf serum and 10 μg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Transcriptome analysis was done using the GeneChip Human Transcriptome Array 2.0 for in combination with the GeneChip WT PLUS Reagent kit (Applied Biosystems). 33,3ng total RNA was used as input for the analysis that was done according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... ELISAs for total IgM or IgG were also performed in parallel using human uncoated IgM or IgG ELISA kits (Invitrogen), following manufacturer’s instructions.
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bioRxiv - Biophysics 2024Quote: ... Secondary antibodies include donkey anti-human IgG Alexa Fluor 555 (1:600) and Goat anti-mouse IgG Alexa Fluor 405 (1:500, Invitrogen). Confocal microscopy was performed using a laser scanning confocal microscope (LSM 880 ...
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bioRxiv - Biochemistry 2024Quote: The purified full-length human and mouse CNPase (hCNPase and mCNPase) were immobilised overnight at +4 °C on a 96-well immunosorbent plate (Nunc). All the following steps were done by gentle shaking on an orbital shaker ...