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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The CF cell line CFBE41ō (ΔF508/ΔF508) and normal human bronchial epithelial cells16HBEō (from D. Gruenert) were maintained in complete MEM-media (GibcoBRL, Life Technologies, MD). The normal human tracheal cell line 9HTEō ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Immunology 2021Quote: ... IgA or IgM antibodies were detected by horseradish peroxidase-conjugated goat anti-monkey/human IgG (γ-chain specific, catalog no. 62-8420, Thermo Fisher), IgM (μ-chain specific ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Genomics 2021Quote: Human brain tissues were manually dissected into small 2-3 mm3 pieces and immersed in homogenization buffer (HBSS (Life Technologies, 14175-095), 1% bovine serum albumin (Sigma-Aldrich ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... The number of viral genome copies for the strain specified in the experiment was spiked into 50 ng of Universal Human Reference RNA (ThermoFisher Scientific #QS0639) to the final volume of 11 μl ...
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bioRxiv - Cancer Biology 2020Quote: Tumor samples with sufficient high-quality RNA (n=5) were analyzed on the Affymetrix GeneChip Human Genome U133 Plus (v.2.0) Array (Affymetrix, Santa Clara, USA) at the Microarray Department of the University of Amsterdam ...
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bioRxiv - Microbiology 2020Quote: ... All parasites obtained from other sources or generated in this study were maintained under standard conditions (37) at 2% hematocrit with O+ human erythrocytes in RPMI1640 (Thermo Fisher Scientific) containing hypoxanthine ...
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bioRxiv - Cell Biology 2022Quote: ... media was collected at the end of 48 h of lipid loading and albumin concentration was measured with a commercially available human albumin ELISA kit (Invitrogen, Waltham, MA).
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bioRxiv - Cell Biology 2022Quote: ... Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (#C10428) and 1-Step Human Coupled IVT Kit – DNA (#88882) were obtained from Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2022Quote: ... Purified molecules or mouse anti-human IL-15 antibodies were coated on a MaxiSorp™ 96-well flat-bottom ELISA plate (ThermoFisher Scientific) for 12h and for 72h ...
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bioRxiv - Immunology 2022Quote: ... 11D5-3 single chain variable fragment (scFv)3 and the human heavy-chain-only FHVH3316 were codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific). Retroviral constructs encoding APRIL CARs ...
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bioRxiv - Immunology 2023Quote: The coding sequence of wild-type human CXCR4 was synthesized by General Biol (Chuzhou, China) and was cloned into the pFastBac transfer plasmid (Invitrogen, Carlsbad, CA). Heat-stabilized BRIL ...
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bioRxiv - Neuroscience 2022Quote: ... similarly as described previously.[60] MS data were analyzed using an in-house maintained human protein sequence databases using SEQUESTTM and Proteome DiscovererTM (Thermo Fisher Scientific). The mass tolerances of a fragment ion and a parent ion were set as 0.5 Da and 10 ppm ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs were plated onto 6-well dishes coated with Matrigel (Thermo-Fisher Scientific; Waltham, MA, USA; Cat. No. 08-774-552) in mTeSR1 medium (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Developmental Biology 2022Quote: ... or miR-483-3p 2⍰-O-Methyl antagonist (anti-human; 0, 50 and 100⍰nM in HepG2) using the Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific – 13778100). Cells were harvested and the luciferase assay was performed as previously described21 using the Dual-Luciferase Reporter Assay System (Promega – E1910) ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from these transfected cells and sent to the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet for Human Clariom™ S assay (ThermoFisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used (all at 1:100 dilution in immunofluorescence, 1:1000 in immunoblots): CX36 mouse anti-human (Invitrogen, clone 1E5H5), rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662) ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic®) and subcloned into pENTR (Life technologies®). GST- ...
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bioRxiv - Cell Biology 2024Quote: Primary or secondary antibodies were purchased from the following vendors: rabbit anti-human ROBO1 (PA5-29917, Thermo Fisher Scientific, Rockford, IL, USA); rabbit anti-human ROBO1 (HPA052968 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... from Illumina and the Axiom™ Genome-Wide Human Origins (HO) and the Axiom™ Precision Medicine Diversity Array Plus Kit (PMDA) from ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... of the human BPTF was amplified by PCR and subsequently cloned into the pDEST-566 vector using Gateway technology (Thermo Fisher Scientific). The resulting 6x-MBP-hBPTF (701-1073 ...
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bioRxiv - Immunology 2024Quote: ... washed in PBS/T and then incubated with 50 µL γ-chain specific goat anti-human IgG-alkaline phosphatase (AP) (Thermo Fisher) at a 1/2000 dilution for 1 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and C-X-C motif chemokine 11 (CXCL11) ELISAs were performed using basal supernatants collected 48 hours after cytokine treatments using IL-8 Human ELISA Kit (ThermoFisher, Cat# KHC0081) and Human CXCL11/I-TAC Quantikine ELISA Kit (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in 1 ml medium per well of a 24-well plate and stimulated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific), IL-7 (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... Human LRRK2 G2019S and LRRK2 Knock-out (KO) iPSC lines were generated in human iPSCs obtained from a female clone from Thermo Fisher (#A18945). Sequence information for generating targeting gRNA ...
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bioRxiv - Microbiology 2023Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37◦ C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... at the final concentration of 5 µM and one of the following anti-human mouse monoclonal antibodies (5 µl per sample) conjugated with allophycocyanin (APC): anti-CD9 (clone MEM-61, Thermo Fisher Scientific), anti-CD63 (clone MEM-259 ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Stable cell lines were transfected with an expression vector containing the full-length cDNA coding for specific human sodium channel α-subunit using Lipofectamine (Thermo Fisher). The NaV1.x stable cell lines and accessory constructs used correspond to the following GenBank accession numbers ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed with the respective human-specific sense and antisense primers and RT-SYBR™ Green PCR Master Mix (Applied Biosystems). Multiwell plates of 384 wells were run on a Viia 7 PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: The region of the elephant shark genome homologous to human CNE1426 was amplified by PCR and subcloned pCR™8/GW/TOPO® (Invitrogen) (the primer sequences are in Extended Data Table 1) ...
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Cryo-electron tomography of eel sperm flagella reveals a molecular “minimum system” for motile ciliabioRxiv - Cell Biology 2023Quote: ... Danio rerio (zebrafish), Mus musculus (mouse), and human proteomes using the CPFC (Trudgian et al., 2010) or Proteomics Discoverer (Thermo Fisher Scientific) programs ...
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bioRxiv - Cancer Biology 2023Quote: Knockdown of PR130 in murine and human PDAC cells was performed by transfecting 30 pmol of validated siRNA molecules targeting the PPP2R3A/Ppp2r3a mRNAs (murine: Santa Cruz, sc-108917, 10 µM stock solution; human: Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), human/mouse Foxp3 (clone 1054c ...
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bioRxiv - Bioengineering 2023Quote: ... To create SiRPα-Fc fusion soluble proteins the cDNA sequence of the full extracellular domain (aa 27-371) of human wild-type allele 1 SiRPα (wtSiRPα) cDNA (Uniprot: P78324) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1 ...
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Immunology 2023Quote: ... and next intracellularly stained with PE-conjugated anti-human phospho-Akt (Ser473) (D9E, CST) and anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) manually conjugated with Alexa 488 or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed twice with PBS + 1% BSA and stained with 10 μg/mL Alexa Fluor™ 647 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (ThermoFisher Scientific) diluted in PBS with 1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...