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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Human liver specimens were minced to extract genomic DNAs (gDNAs) using the PureLink™ Genomic DNA Mini Kit (Invitrogen™, K182001), and genomic DNA concentrations were measured using a NanoDrop™ 2000/2000c spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Molecular Biology 2024Quote: Cell line derived from human neuroblastoma (SH-EP; CVCL_RR78) was verified by STR profiling and grown in RPMI-1640 (Thermo Fisher Scientific). Murine neuroblastoma cells (NHO2A ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... followed by coating with 5 µg/mL of human recombinant laminin 521 (BioLamina, #LN521-02) in DPBS (with calcium and magnesium, Gibco #14040117) overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: An adenoviral construct encoding human aSyn A53T-mVenus was produced using the Vi-rapower Adenoviral Expression System from Invitrogen (Carlsbad, CA). The aSyn A53T-mVenus cDNA was subcloned as a KpnI-XhoI fragment into a variant of the entry vector pENTR1A carrying the human synapsin promoter (h-syn-P).48 Phusion High-Fidelity DNA polymerase or Q5 Hot Start High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... One μg of plasmid was electroporated into 200,000 human primary myoblasts carrying the Δ52 DMD mutation using the Neon® transfection system (Life Technologies, Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... FITC-conjugated mouse anti-human CD4 (Clone OKT4) Antibody (1:500 dilution) and AquaVivid viability dye (Thermo Fisher Scientific, Cat# L43957) for 20 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Strains were then inoculated at an OD600 (optical density at 600 nm) of 0.1 in pooled human urine (Mutiskan™ GO Microplate Spectrophotometer, Thermo Scientific), producing 200 µL bacterial cultures on a 96-well plate ...
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bioRxiv - Microbiology 2024Quote: ... Wells were triple washed in PBST and incubated with goat anti-human IgG conjugated to streptavidin horse radish peroxidase (HRP) (#A18811, ThermoFisher Scientic) for 1 h at RT under agitation ...
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bioRxiv - Microbiology 2024Quote: ... Phalloidin iFluor 647 (1:100, #23127-AAT, Stratech) (F-actin) and goat anti-human IgG Alexa555 (1:200, #A-21433, ThermoFisher Scientific) for 1 h at RT in the dark ...
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bioRxiv - Microbiology 2024Quote: ... 0.01 ng/ml human epidermal growth factor (Pep Rotech, AF-100-15) and 1x Penicillin-Streptomycin (Gibco™, Cat. No. 15140122).
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bioRxiv - Neuroscience 2024Quote: Staining of human Myc tag in brain and spinal cord sections was done using either the 9E10 purified Mab (Invitrogen #132500), or 9E10 hybridoma supernatant as primary antibodies ...
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bioRxiv - Genomics 2024Quote: ShP51 cells were transfected with control siRNA (Silencer Select Negative Control #1) or human TSKU siRNA (Silencer Select standard siRNA s24880) from Thermo Fisher Scientific using Lipofectamine RNAiMAX (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: Soluble immune checkpoints were measured with the 14 plex ProcartaPlex™ Human Immuno-Oncology Checkpoint Panel 1 (Invitrogen, Waltham, MA, USA) using Bio-Plex® 200 technology (Bio-RAD ...
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bioRxiv - Synthetic Biology 2024Quote: ... the plate was washed three times and incubated for 1 h with 100 μL of F(ab’)2-goat anti-human IgG (H+L) antibody conjugated to HRP (1:5,000 dilution; ThermoFisher, Cat # A24464). After three washes ...
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bioRxiv - Immunology 2024Quote: ... was cloned from full-length human PLA2G12A cDNA (provided by Dr. G. Lambeau) in the modified pcDNA3.4 vector (Thermo Fisher Scientific) harboring an N-terminal human IL-2 signal sequence and C-terminal mIgG2aFc (Fc of mouse IgG2a) ...
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bioRxiv - Immunology 2024Quote: Human PBMCs were cultured in RPMI-1640 supplemented with 10% FBS and activated with DynabeadsTM Human T-Activator CD3/CD28 (Gibco, 11132D). One day post activation ...
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bioRxiv - Molecular Biology 2024Quote: ... Relative cDNA abundance for Wnt gene expression was quantified using a TaqMan Human Wnt Pathway Array (Applied Biosystems, Foster City, CA) via probe hybridization and chemiluminescence detection.
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bioRxiv - Neuroscience 2024Quote: ... chemokines and growth factors in 14 supernatant samples by Luminex using the Cytokine Human Magnetic 30-Plex Panel LHC6003M from Life Technologies™ ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Genomics 2024Quote: ... along with the N-terminal peptidase domain of human ACE2 was expressed using the Bac-to-Bac® baculovirus system (Invitrogen). The RBD (Residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2024Quote: MRC-5 human embryonic lung fibroblasts were purchased from ATCC (Manassas, VA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco Thermo Fisher) supplemented with 10% FBS (Gibco Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Neuroscience 2024Quote: Primary mouse and human iPSC-derived astrocytes were incubated with Fluo-4 calcium assay reagent (Fluo-4 Direct™ Calcium-Assay-Kit, ThermoFisher) for 30 min at 37°C before recording baseline fluorescence levels (RFUMin ...
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bioRxiv - Neuroscience 2024Quote: ... or 2% normal goat serum in 0.2–0.3% Triton X-100 in PBS: anti-human tau (clone HT7) (ThermoFisher, MN1000; 1:50), anti- MAP2 (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... The expression plasmid encoding full-length human AIFM1 was synthesised and ligated in the pcDNA3.1(+) backbone by GeneArt (Thermo Fisher Scientific). Insert sequences are provided in the Supplemental Material.
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bioRxiv - Bioengineering 2024Quote: ... Normal human dermal fibroblasts (NHDFs) were purchased from KURABO and maintained in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, MA, USA) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were adjusted to a concentration of 1*10^6 1/mL and cultured together with Dynabeads human T-Activator CD3/CD28 beads (Gibco, Germany) according to the manufacturers instructions for 3 days ...
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bioRxiv - Cell Biology 2024Quote: ... They were cultured in Keratinocyte serum-free medium (SFM) supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (17005042, Life Technologies) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... human CENP-E ON-TARGETplus SMART pool siRNA (L-003252-00-0010, human custom-made 3’UTR HEC1 siRNA (oligo #3, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Two SilencerTM Validated siRNAs along with an ON-TARGET Plus Human siRNA smartpool (Horizon Discovery) for each gene of interest were purchased (Invitrogen, Thermo Fisher).
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with eBioscience Flow Cytometry Staining Buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 1 h in ice ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using TaqMan Advanced miRNA Assary (Assay ID: 477887_mir, for has-miR-126) and TaqMan Advanced miRNA human endogenous control (Thermo Fisher Scientific) with Quantstudio 3 (Applied biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... incubated at 37°C for 48 h prior to CD3 isolation and activation with human T-Activator Dynabeads (Life Technologies 11131D) at a 3:1 bead:cell ratio for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies for immunohistochemistry were mouse anti-human caspase-8 (clone B.925.8; RRID:AB_10978471; 0.619 g/L Thermo Fisher Scientific Cat# MA5-15226), rabbit anti-cleaved-caspase-3 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Human HEK-293T were commercially acquired (cat. no. CRL-11268, ATCC) and grown in Advanced DMEM (cat. no. 12491015, ThermoFisher Scientific) medium supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... that were previously coated for 1 h at room temperature with 6 mg/mL human fibronectin diluted in PBS (ThermoFisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... incubated with the cell marker Cell Mask (Invitrogen) for 20 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cell Trace™ CFSE Cell Proliferation (Life Technologies) and Violet Proliferation Dye 450 (VPD450 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with Cell Trace Violet (Invitrogen) on the day of transfection ...
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bioRxiv - Microbiology 2021Quote: ... 2×106 cells/ml EXPI293F cells (Life Technologies) were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were detached using cell dissociation agent (Invitrogen) and 2% of the cell suspension was distributed per well of a 96-well plate ...
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bioRxiv - Immunology 2024Quote: ... cells were labelled with Cell Trace (ThermoFisher Scientific) and stimulated with microbeads coated anti-mouse CD3/ CD28 (Dynabeads mouse cell activator) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were counted (Countess Automated Cell Counter, ThermoFisher) and ...
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bioRxiv - Pathology 2024Quote: ... lepidopteran ‘SF9’ cells (Gibco™ Sf9 cells, Invitrogen) were transfected at approximately 85-90% confluency ...
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bioRxiv - Pathology 2024Quote: ... lepidopteran ‘SF9’ cells (Gibco™ Sf9 cells, Invitrogen) were transfected at approximately 85-90% confluency ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells (Invitrogen) and cultures grown in LB media ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended at 1×106 cells/ml in T cell media [RPMI-1640 (Gibco) + 10%FBS + 1% penicillin/streptomycin + 40μM 2-β-mercaptoethanol (Sigma-Aldrich)] ...
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bioRxiv - Bioengineering 2022Quote: ... MM cells and T cells were stained with Cell Trace Far Red dye (CTD, Invitrogen) according to the manufacturer’s instructions ...