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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... # 236-EG), 20 ng/ml recombinant human FGF basic protein (R&D, # 4114-TC),1% penicillin/streptomycin (P/S, Invitrogen, SV30010), 1% sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and ME49 hxgprt-ko ku80-ko (ME49) were grown in confluent human foreskin fibroblasts (HFF, ATCC SCRC-1041) in DMEM (Gibco 41965039) supplemented with 5% fetal calf serum and 40 μg/ml gentamycin (Gibco 15710-049) ...
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bioRxiv - Immunology 2020Quote: Human iPSC-derived microglia (Tempo-iMG™; Tempo Bioscience, CA) was cultured in DMEM/F-12 (Thermo Fisher, Waltham, MA, USA) supplemented with 1X N2 supplement ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated overnight and IFNγ levels in the supernatant were detected using the human IFN-γ ELISA Ready-SET-Go! kit (Invitrogen) accorded to manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... The IgG from human sera or MuSK-specific mAb 4A3 and AChR-specific mAb 637 were captured with AG beads (Thermo Fisher) and then eluted by boiling at 95 degrees in 2X Laemmli buffer (with 10% beta-mercaptoethanol) ...
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bioRxiv - Immunology 2020Quote: ... The secondary antibodies goat anti-rabbit IgG H&L (HRP) (GE NA934,1:4000) and HRP-labeled goat anti-human IgG (gamma chain) cross-adsorbed secondary antibody (Invitrogen, 62-8420) (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... populations were subject to either “rest” (low dose 30U/mL IL-2 only) or stimulation using Dynabeads Human T-Expander CD3/28 (ThermoFisher Scientific) at 1 million cells/mL at a ratio of 2 beads per cell for 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Sybr green assays were also used for human or mouse gene expression with SYBR Green Master Mix (2x, Thermo Fisher Scientific). Primer sequences are as follows:
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bioRxiv - Neuroscience 2021Quote: ... From live single cells with top 30% protein expression (evaluated by EGFP signal) the MFI of the Alexa Fluor 647-conjugated goat anti-human IgG (1:400, Life Technologies) was calculated.
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted and the whole transcriptome of ALDH+ and ALDH-populations was assessed using Affymetrix Whole-Transcript Human Gene 1.0 ST Array (Affymetrix, Thermo Fisher Scientific) (30) ...
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bioRxiv - Neuroscience 2022Quote: Healthy wild type control (WT) iPSCs derived were obtained from Thermo-Fisher Scientific (WT, GIBCO human episomal iPSC line, cat # A18945), which were generated via reprogramming of cord blood derived CD34+ progenitors ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were maintained in monolayer cultures of human foreskin fibroblasts (HFFs, ATCC® SCRC-1041) in Dulbecco’s Modified Eagles Medium (DMEM, GIBCO, USA) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... FLAG-tagged human DNA2 nuclease-deficient (D277A) and helicase-deficient (K654R) mutants were generated by Phusion site-directed mutagenesis (Thermo Fisher) of wild-type DNA2-FLAG (pIF494 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5.5μg of fragmented cDNA was hybridized on Affymetrix GeneChip™ Human Transcriptome Array 2.0 (HTA2.0) chips (Invitrogen, 902162, lot no. 4364589) for 16 hrs at 45°C ...
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bioRxiv - Cell Biology 2022Quote: ... Transcriptional profiling was performed with the use of the Affymetrix Human Genome U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA). All labeling reactions and hybridizations were carried out according to the standard GeneChip® eukaryotic target labeling protocol ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat polyclonal antibodies conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/100 in FACS buffer ...
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bioRxiv - Genomics 2022Quote: PBMC culture supernatants were analyzed for secreted proteins with Immune Monitoring 65- Plex Human ProcartaPlex Panel (Thermo Fisher Scientific, Carlsbad, CA) as per manufacturer’s instructions (all proteins assayed shown in Table S5) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 pmol of desthiobiotinylated HOXC13-AS was mixed with magnetic beads and incubated with 200 µg of protein lysate from human adult epidermal keratinocytes (ThermoFisher Scientific). Poly(A)25 RNA ...
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bioRxiv - Cell Biology 2022Quote: ... established mature DB-VOs at day 15 from diabetic iPSCs were started to culture in high glucose concentration (33mM, adapted from our previous publication (Yang et al., 2020)) plus 1 ng/ml human TNF (Invitrogen, PHC3011) and 1 ng/ml IL-6 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA levels were normalized to mouse Gapdh or human RPL22 cDNA and relative transcription levels were analyzed using the comparative ΔΔCt method (7500 Software V2.0.5, Applied Biosystems, Life Technologies). The Lgmn mRNA level of the microglia-enriched fraction was obtained from a NanoString dataset (Omnibus GSE129709 https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE129709)10.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed 3x in FACS Buffer and stained on ice for 1 hour with goat anti-Human IgG Fc Alexa Fluor™ 647 secondary antibody (ThermoFisher), protected from light ...
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bioRxiv - Pathology 2024Quote: ... and slides were analyzed independently by 3 experienced reviewers to identify and measure the area of human cardiomyocyte grafts using an EVOS (Life Technologies) microscope ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... samples were washed in PBS and a mixture of goat anti-Human Alexa488 conjugated secondary antibody (Thermo Fisher, diluted 1:2000) and DAPI (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Microbiology 2024Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Approximately 800,000 U2OS cells were reverse transfected with 20 nM siRNA against the 3’ UTR of endogenous TIRR or a negative control siRNA (Dharmacon ON-TARGETplus Human NUDT16L1 siRNA J-014872-23-0005 or ON-TARGETplus Non-targeting Control) using 3 uL of RNAiMAX lipofectamine (Thermo Fisher) in a 6-well plate ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20 ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Biophysics 2023Quote: ... University of Belgrade-Faculty of Biology which was previously produced by several reactions of cloning and subcloning of CTG repeats originating from human DMPK locus using pJet1.2/blunt cloning vector from CloneJET PCR Cloning Kit (Thermo Fisher Scientific). The subcloned region sequence was verified with Sanger sequencing before prior use ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were then transfected with 25 nM of either ON-TARGETplus Human ABCB1 (5243) SMARTpool or non-targeting pool siRNAs (negative control, Dharmacon) using the Lipofectamine RNAiMAX reagents (Thermo Fisher). Following 24 hours of incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... human EGF (10 µg/ml) and bovine insulin (10 µg/ml) in low-attachment plates (Nunclon sphera, Thermo Scientific™ #174932) and maintained at 37°C in a humidified 5% CO2 incubator for 3 days before harvesting ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D7 blood-stage cultures were maintained at 4% hematocrit in human A+ erythrocytes in Rosewell Park Memorial Institute (RPMI) 1640/Hepes medium (Gibco, 11875119) supplemented with 0.5% Albumax II (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... a human brain organoid cultured during 4 months has been dissociated in TRIzol RNA isolation reagent (Thermo Fisher Scientific ref: 15596026). Extracted RNA has been processed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 million SH-SY5Y cells stably overexpressing human A53T mutated α-synuclein (Danzer et al., 2007) were resuspended in electroporation buffer R (NEON electroporation system, ThermoFisher) and added to the antibody-fibril preparation (final volume 100 μl) ...