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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Cryo-preserved primary human hepatocytes (PHH) from single donors (BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; and Thermo Fisher Scientific) were used for all in vitro experiments ...
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bioRxiv - Immunology 2022Quote: ... in culture media or stimulated for 48h with α-CD3/CD28 coated beads (Dynabeads Human T-Activator, Thermo Fisher scientific) in a 1:1 ratio (beads to cells) ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: Toxoplasma gondii RH∆hxgprt strain was maintained by growth in confluent primary human foreskin fibroblasts (HFFs) in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... were designed and used to create three different human-yeast hybrid genes by PCR (AccuPrime Pfx DNA polymerase from Invitrogen). The primers also add the attB1 and attB2 sites at the 5’ and 3’ ends of the PCR for cloning in pDONR221 entry clones using the BP cloning strategy ...
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bioRxiv - Immunology 2022Quote: ... Sorted Treg cells from HD and IPEX patients were stimulated with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
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bioRxiv - Genomics 2022Quote: Purified human and bacterial DNA were quantified for concentration with the Qubit dsDNA HS assay (Life Technologies, Q33231, MA, USA), and pooled by adding 94% of human DNA ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: Telomerase-transduced Human Foreskin Fibroblasts (HFFs) and MRC5 lung fibroblasts were cultured in Dulbecco’s modified Eagle serum (DMEM; Invitrogen #11965118) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Molecular Biology 2022Quote: ... and 24 hours later cells were transfected with the Bluescript vector (empty vector control) and Human L1 plasmid (used in L1 retrotransposition assay) using Lipofectamine 2000 reagent (Invitrogen). Cells were harvested 36 hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...
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bioRxiv - Molecular Biology 2022Quote: ... 2×105 cells were transfected with 20 pmol of ON- TARGETplus Human HMG20A (10363) siRNA-SMART pool (Dharmacon) using Oligofectamine™ according to the manufacturer’s instructions (Invitrogen). Cells were cultured for 3 days before being harvested for follow-up experiments.
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bioRxiv - Neuroscience 2022Quote: ... human BDNF-biotin (Alomone labs; B-250-B) was conjugated to Quantum dots-605 (QD-605) (Thermo Fisher Scientific; Q10103MP). Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... the RAD54L2 cDNA was assembled from a partial cDNA clone encoding Met109 to Lys1467 was from the human ORFeome collection (Lamesch et al, 2007) and a synthetic DNA fragment encoding Met1 to Glu108 (Invitrogen). RAD54L2K311R was constructed by QuikChange site-directed mutagenesis with primers (5’-AGATCACTTGCAAAGTTCTCCCCAGACCCATGCTG-3’ and 5’-CAGCATGGGTCTGGGGAGAACTTTGCAAGTGATCT-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: Organoid culture supernatants and lysates were analyzed for secreted proteins using single- and multiplexed Human ProcartaPlex™ Panel (Thermofisher Scientific), following previously documented procedures.37,38 The plates were read using the Luminex MAGPIX xMAP instrument and xPONENT® software ...
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM GlutaMAXTM (4.5 g/L D-glucose) (Life Technologies #10-566-024) supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The Pro-Pol fusion HA-tagged (ProWTPolWT and ProMUTPolWT) plasmids were generated using an in-house human PGK promoter-driven lentiviral vector via Gateway cloning (Invitrogen). Plasmids expressing HuSaV (GI.2 ...
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bioRxiv - Cell Biology 2024Quote: ... The process entailed cloning the various human ACTL7B gene variants into the pENTR TOPO vectors using the pENTR d-Topo cloning kit (Invitrogen), following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish Kcnc3a channel was labeled using a polyclonal antibody originally generated against the human Kcnc3 protein (ThermoFisher Scientific PA5-53714) at a concentration of 2.5 μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated human Anti-CD9 antibody (MEM-61; catalog no. MA1-19485) and biotinylated human anti-CD81 antibody (M38; catalog no. ab239238) were purchased from Thermofisher Scientific (Stockholm ...
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bioRxiv - Cell Biology 2024Quote: ... the mucosal layer was removed from an approximately 1 cm3 fresh section of human colonic tissue and washed twice with 1X Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher, 14040133). The mucosa was transferred to a 5-ml conical tube containing 0.5 ml crypt isolation buffer (2 mM EDTA ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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bioRxiv - Immunology 2024Quote: ... Bound mAb was detected using goat anti-human (H+L)-HRP (1:1000 dilution) (Thermo Fisher Scientific, Waltham, MA, USA). In the case of His-tag detection ...
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bioRxiv - Bioengineering 2024Quote: Human BJ fibroblasts (ATCC CRL-2522) were cultured on standard cultureware in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco 11965-092) with 10% Fetal Bovine Serum (FBS ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... Acquired spectral data files were searched against the human component of UniProtKB/SwissProt database using SEQUEST search engine in the Proteome Discoverer 1.4 (Thermo Scientific). The following parameters were applied ...
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bioRxiv - Microbiology 2023Quote: ... a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (1:500 dilution; #43700, Invitrogen) and a peroxidase-conjugated goat anti-mouse secondary antibody (1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... A 10% (w/v) solution of biomaterial was prepared by manually mixing the pulverized biomaterial in Human endothelial serum free media (HESFM; Gibco) supplemented with fetal bovine serum (2% v/v ...
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bioRxiv - Developmental Biology 2023Quote: A small piece of primary human or mouse tissue was resected and embedded in Fisher Healthcare Tissue-Plus Optimum Cutting Temperature (OCT, ThermoFisher). This was frozen at -80 0C ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Cancer Biology 2023Quote: The 387 complex genetics sarcomas were analyzed on Human Genome U133 Plus 2.0 array (900466, Affymetrix, Santa Clara, CA, USA), according to the manufacturer’s procedures ...
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bioRxiv - Systems Biology 2023Quote: 1000-3000 IEQ human islets pellets were resuspended in 200 µL PBS and treated with 20 µL 10 mg/mL Rnase A (Invitrogen) and 20 µL Protein Kinase K (Qiagen ...
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bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: RT-qPCR with TaqMan primer/probes was used to determine relative expression levels of miR-146a compared to U18 (human) or sno251 (mouse) endogenous control genes (ThermoFisher, assay IDs ...