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Citations for Thermo Fisher :
7201 - 7250 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Bioengineering 2023Quote: ... isolated naïve CD8+ T cells were plated at 2.5 × 105 cells/well in a 24-well plate in 2 ml cRPMI on day 0 and activated with DynaBeads Human T-Activator anti-CD3/CD28 coated microbeads (Thermo Fisher Scientific) and recombinant human IL-2 (rIL-2 ...
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bioRxiv - Neuroscience 2022Quote: ... RNA quality and quantity were assessed and samples with RINS of 7 or greater were deemed suitable for further analyses with the Affymetrix Human Exon 1.0 ST Array according to the manufacturer’s instructions (Affymetrix, Santa Clara, CA, USA). After hybridisation ...
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bioRxiv - Cell Biology 2023Quote: ... E6 medium was changed daily up to 4 days before switching to human endothelial serum free medium (hESFM) (Thermo Fisher Scientific, 11111) supplemented with 10nM bFGF (Fibroblast growth factor ...
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bioRxiv - Cell Biology 2022Quote: ... human FLOT2 (ON-TARGETplus Human FLOT2 siRNA SMART POOL, L-003666-01-0010) (Horizon Discovery Ltd.), non-targeting control siRNA (#4390843, 40 nM) (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2023Quote: ... the mice received an intravitreal injection of either human monocytes or hMdɸs that were labeled with the Vybrant DiO tracer (Invitrogen-Molecular Probes, Carlsbad, CA), and then returned to the standard light/dark cycle ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times with PBS and labeled with secondary antibodies (goat anti-rabbit/human conjugated to Alexa Fluor 647; 1:1000; Invitrogen, #A21246/A21445) and FluoTag-X4 anti-GFP conjugated to Atto488 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Cell Biology 2023Quote: ... seeding media was exchanged for fresh media containing 10% human serum from pregnant or non-pregnant donors together with EdU (Click-iT™ EdU Proliferation Assay for Microplates, ThermoFisher Scientific) and cultured for 1 week ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), anti-human/mouse Foxp3 Ab pre-labeled with Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... ATCC TIB-202) or primary human monocytes (PHMs) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 10% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Developmental Biology 2024Quote: The targeted transcriptome sequencing was performed on the Ion S5XL Next Generation Sequencing system with the Ion AmpliSeq Transcriptome Human Gene Expression Assay (ThermoFisher Scientifics Inc). This assay covers 20,802 human RefSeq genes (>95% of UCSC refGene ...
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bioRxiv - Microbiology 2023Quote: ... medium was changed to differentiation medium containing a 1:1 mixture of IntestiCult™ OGM Human Basal Medium (#100-0190) and Advanced Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, ThermoFisher Scientific, 12634-028) supplemented with 1% Pen-Strep ...
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bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and a 1:1000 dilution of goat anti-human IgG (γ-chain specific) alkaline phosphate conjugate antibody (A3187, Thermo Fisher) was added and incubated for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37°C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2mM L-glutamine ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Immunology 2024Quote: ... The concentration of hIL-1β and hIL-8 were measured using a human Invitrogen ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA). All the protocols were used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Cryopreserved primary human hepatocytes were genotyped for PNPLA3 rs738409(G) using TaqMan® SNP Genotyping Assays (Life Technologies, Assay ID C_7241_10, 4351379) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were transferred to gelatinized slides and immunofluorescence staining was performed using a human CD45 antibody (Rat. YAML501.4; Thermo Fisher Scientific, USA), GFP Polyclonal Antibody (A-11122 ...
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bioRxiv - Cell Biology 2024Quote: ... one million human iPSCs were thawed and resuspended with 1 mL of Dulbecco’s Modified Eagle Medium F12 (DMEM F12) media (Thermofisher, Catalog No. 12634010) then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf ...
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bioRxiv - Cell Biology 2024Quote: The pTP6-3xFlag-KDM6A was obtained through EcoRI digestion and subcloning into the pTP6 vector59 of the 3xFlag-KDM6A sequence optimized for human expression (purchased from Thermo Fisher Scientific). The cDNA encoding for human ZFX was purchased from Genescript ...
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bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... human MSCs were isolated from the UC (Wharton’s Jelly) and expanded with proliferation medium (Minimum essential Medium-α (MEM-α) (Gibco™) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... The respective mouse and human brain sections were subsequently incubated with Invitrogen donkey anti-rabbit Alexa Fluor 488 (Catalog No. A-21206; Invitrogen, Carlsbad, CA) for VGLUT2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
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bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Human slices were routinely stained with anti-NeuN (diluted 1:300 from stock; Rabbit anti-NeuN, Thermo Fisher Scientific PA5-37407, RRID:AB_2554049), visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock ...
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bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific, U.S.A.] with B27 Supplement [Thermo Fisher Scientific, U.S.A.]), then transferred to the hiPSC-derived cardiomyocytes for 48 hours of treatment either in the presence or absence of 100ng/mL of dexamethasone (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Human monocyte-derived macrophages (hMDM) were cultured in Nunc™ MicroWell™ 96-well optical-bottom plates (Thermo Fisher Scientific, Waltham, MA) and left untreated or treated with vehicle (sterile 1X PBS) ...
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bioRxiv - Bioengineering 2024Quote: ... flow throughs and washes from StEVAC method were suspended in 20 µl of Exosome-human CD81 flow detection beads (Thermo Scientific, 10622D) and incubated at 4°C overnight while rotating ...
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bioRxiv - Synthetic Biology 2024Quote: Total secreted HSA concentrations of 72 hr yeast HSA expression cultures were quantified via commercially available Albumin Human ELISA Kit (ThermoFisher Scientific #EHALB), following the included protocol ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Fixable Viability Dye eFluor 780 and the following monoclonal antibodies: TexasRed-PE conjugated anti-human CD3 (clone 7D6, Invitrogen, cat. MHCD0317); PerCP/Cyanine5.5 conjugated anti-human CD4 (clone OKT4 ...
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bioRxiv - Microbiology 2024Quote: Supernatant collected from human tissues at 48 or 72 hpi after infection with RVFV ZH501 or LAVs (ΔNSs, ΔNSm, MP-12, ΔNSs/ΔNSm) was used to detect total human interferon alpha (IFNα; Invitrogen catalog #BMS216) and human interleukin 29 (IL-29/IFN-λ1 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... CR3022 antibody (10 μg/mL) and 10 μg/mL of goat anti-human IgG (H+L) secondary antibody Alexa Fluor 647 (Invitrogen #A-21445) was pre-incubated for 30 min at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... and further subcloned into pAAV2 under the control of the human synapsin promoter and flanked by lox71 and lox66 sites using EcoRI and BamHI restriction sites (FastDigest, Thermo Fisher Scientific). The constructs were soma-targeted using the Kv2.1 targeting domain.
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in Live Cell Imaging Solution (Invitrogen) for 2 hours prior to incubation in experimental nutrient combinations ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were imaged in Live Cell Imaging buffer (Invitrogen) supplemented with 20⍰mM glucose ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were maintained in Live Cell Imaging Solution (Invitrogen) at 37°C in 5% CO2 during image acquisition and microscope acquisition parameters for all images were identical ...
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bioRxiv - Systems Biology 2021Quote: ... Myogenic cells were passaged with Cell Dissociation Buffer (Gibco).
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bioRxiv - Cell Biology 2021Quote: Cells were imaged in Life Cell Imaging buffer (Invitrogen) supplemented with 20 nM Glucose (Merck 1.08342.1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultivated in cell culture incubator (Thermo Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were filtered through 70 μm cell strainers (ThermoFisher) and applied onto a 40%:80% Percoll gradient (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... cells were labelled with cell trace violet (Thermo Fisher) or cell trace yellow (Thermo Fisher ...