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Citations for Thermo Fisher :
6701 - 6750 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: Two biological replicates of RNA samples were isolated from A549 cells transduced with pMSCV-dnCRTC or pMSCV-GFP retroviruses at 72 hours post-infection and were then subjected to microarray experiment using GeneChip® Human Transcriptome Array 2.0 (Affymetrix) at the Genomics Core at Sanford Burnham Research Institute ...
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bioRxiv - Cancer Biology 2021Quote: ... Gene expression data was collected using the Gene Chip® Human Genome U133 Plus 2.0 Array (Affymetrix, Santa Clara, CA) protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were incubated with anti-human IgG1-AlexaFluor 594 or streptavidin Alexa Fluor 594 (Life Technologies-Thermo Fisher Scientific) diluted in PBS + 1% BSA buffer for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were incubated with anti-human IgG1-AlexaFluor 594 or streptavidin Alexa Fluor 594 (Life Technologies-Thermo Fisher Scientific) diluted in PBS + 1% BSA buffer for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... ON-TARGET plus human MGEA5 (10724) siRNA-smartpool) or nontargeting siRNA (Dharmacon, ON-TARGETplus nontargeting pool) using Lipofectamine RNAiMAX (Invitrogen) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSC-CMs were washed 3 times with PBS 1X and incubated with the secondary antibodies diluted in blocking solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse Alexa Fluor 488 (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... and ESRG_cn2 (Hs06675423_cn) detected the ESRG locus and TaqMan Copy Number Reference Assay human RNase P (4403326, Thermo Fisher Scientific) was used as an endogenous control ...
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bioRxiv - Cell Biology 2020Quote: ... and target genes were amplified for 12 cycles of PCR using the Ion AmpliSeq Human Gene Expression Core Panel (Thermofisher). This panel contains a pool of 20,802 amplicons (41,604 primers ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were washed and stained for flow cytometry using appropriate seconday antibodies: goat anti-human fluorescein isothiocyanate (FITC; ThermoFisher A18812), anti-mouse Alexa Fluor 488 (Cell Signaling Technology 4408S) ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... from the RUES2 line (RUESe002-A; WiCell) were maintained on recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) in Essential 8 (E8) media (ThermoFisher) at a density of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Paraffin-embedded human tissues and hind paws were cut into 5 μm sections with the Microtome HM355S (Thermo Fisher Scientific) and transferred onto microscope slides.
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bioRxiv - Immunology 2022Quote: For RNA extraction from human lung resection material 50∼100 mg of RNAlater-stabilized lung tissue pieces were added to 1ml of Trizol (Invitrogen) and homogenized ...
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bioRxiv - Neuroscience 2022Quote: DNA coding for full length HA-tagged human NEGR1 (NM_173808.2) was synthesized using the GeneArt® Gene Synthesis service (Life Technologies) and subcloned into the pcDNA3 vector ...
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bioRxiv - Microbiology 2022Quote: J774.1 mouse macrophages (ATCC) or primary neonatal normal human dermal fibroblasts (Neo-NHDF) (ATCC) were cultured in 10 mL DMEM (Gibco, ThermoFisher) in T75 flasks (VWR ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... Globin mRNA was depleted from a portion of each total RNA sample using the GLOBINclear™-Human kit (Thermo Fisher). Following the removal of globin transcripts transcriptome profiles were generated via mRNA sequencing ...
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bioRxiv - Cell Biology 2022Quote: Human and mouse lung samples were inflated with a 4% w/v paraformaldehyde (PFA) solution in PBS (14190250, Thermo Fisher) then placed in 4% PFA overnight for fixation ...
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bioRxiv - Bioengineering 2022Quote: ... The xeno-free epidermal bioink was formulated with 2 x 106/ mL human KCs in 500 μL of EpiLife medium (#MEPI500CA; ThermoFisher) supplemented with HKSdaFREE and HKGE (AvantBio ...
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bioRxiv - Microbiology 2022Quote: Human monocytes/macrophages U937 and Thp1 were cultured in RPMI-Roswell Park Memorial Institute media supplemented with 10% FBS (Gibco) at 37°C in a humidified incubator (Panasonic ...
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bioRxiv - Biochemistry 2022Quote: Chimeric SHh precursor encoding HA-tagged NLuc fused to native and mutant forms of human Sonic HhC autoprocessing domain were assembled in pDisplay (Invitrogen). Fragments encoding Sonic HhC were inserted into pDisplay using PstI and XhoI ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3 times with PBS and incubated for 1 hour with Alexa 488 nm or Alexa 594 nm conjugated secondary antibodies specific for human and rat IgG (Invitrogen) diluted 1:2,000 in 3% BSA in PBS and containing 1 μg/ml DAPI ...
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bioRxiv - Immunology 2022Quote: ... The abundance of select cytokines was then measured in 50μL of the supernatants with a Human Cytokine Magnetic 10-Plex Panel (ThermoFisher, LHC0001M) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Immunology 2020Quote: DNA encoding antibody variable domains was amplified by PCR from the pHEN1 phage display vector and cloned into a human IgG1 expression vector based on pCEP4 (Invitrogen). After validation of the cloning by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: RNA from human cerebral organoids was extracted according to the manufacturer’s protocol supplied with TRIzol reagent (15596018, Thermo Fisher Scientific). The concentration and purity of the RNA samples were measured using Spectrophotometer (Beckman Coulter).
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bioRxiv - Molecular Biology 2021Quote: ... and S309 and VHH72-Fc or variants thereof was detected with an AF633 conjugated goat anti-human IgG antibody (Invitrogen). Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec ...
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bioRxiv - Biochemistry 2021Quote: ... the cells in each well were transfected with 20 pmol siRNA targeting to human XPO1 (GenePharma, Shanghai, China) using lipofectamine 2000 (Invitrogen). At 6 h after transfection ...
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bioRxiv - Molecular Biology 2020Quote: Efficiency of all qPCR probes was tested by using a simplex 2-fold serial dilution of Quantigene commercial reference human RNA (Invitrogen) or DNA (Promega ...
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bioRxiv - Immunology 2020Quote: ... and SARS-CoV-2 and the N-terminal peptidase domain of human ACE2 (residues Ser19-Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously described 18,19,37-39 ...
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bioRxiv - Biochemistry 2020Quote: In vitro 3D epidermal skin equivalents were generated as previously described.[28] Human neonatal keratinocytes (HEKn, ThermoFisher Scientific, Loughborough, UK) were maintained as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: We purified polyclonal IgG from human plasma samples using Pierce™ Protein G Ultra Link™ Resin (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Fibrin formation was visualized by spiking whole blood with human fibrinogen conjugated to Alexa Fluor 647 (Cat. No. F35200, Invitrogen, used at final concentration of 7.5 μg/mL) ...
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bioRxiv - Cancer Biology 2021Quote: ... PLK2 in a pDONR223 vector was obtained from human ORFeome (Open Biosystems-Horizon Discovery) and was cloned into pCW57.1 using the Gateway recombination reaction (Life Technologies) and verified by sequencing prior to transfection ...
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bioRxiv - Microbiology 2021Quote: ADAP1CRISPR and CtrlCRISPR TM were left resting or stimulated with Dynabeads human T-activator anti-TCR/anti-CD28 (Gibco, 11132) (1 bead/cell ...
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bioRxiv - Microbiology 2021Quote: ... and the N-terminal peptidase domain of human ACE2 (residues Ser19–Asp615) were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as described previously21 ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Immunology 2020Quote: ... Acquired DDA .raw files were searched against the human UniProtKB/SwissProt database (v2012_07) using Byonic (Protein Metrics) embedded in Proteome Discoverer (ThermoFisher Scientific) to obtain peptide sequence information ...
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bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
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bioRxiv - Microbiology 2021Quote: Huh-7 cells seeded in 6 well plates were transfected with human WWOX-specific or random siRNAs (DHARMACON) at a final concentration of 50nM per well using Lipofectamine 2000 reagent (INVITROGEN). At 24 hours post siRNA transfection ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies were detected by probing with biotin-conjugated anti-rat antibody (1:1,000) and Alexa 488-conjugated anti-human antibody (1:1,000) followed by Alexa 594-conjugated streptavidin (Invitrogen, 1:1000). Slides were then stained with 1ug/mL DAPI ...
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bioRxiv - Microbiology 2022Quote: ... gondii parasites of the type I RH strain Δku80/Δhxgprt genetic background (ATCC PRA-319) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% inactivated fetal calf serum (IFS ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: A recombinant fragment of human SP-D (rfhSP-D) was expressed under bacteriophage T7 promoter in Escherichia coli BL21 (λDE3) pLysS (Invitrogen), transformed with for plasmid containing cDNA sequences for neck ...
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bioRxiv - Immunology 2022Quote: ... were generously provided by Anita Koshy (University of Arizona) and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco] ...
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bioRxiv - Immunology 2022Quote: ... and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco], 10% FBS [Gibco] ...
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bioRxiv - Immunology 2022Quote: ... and maintained by serial passage through human foreskin fibroblast (HFF) monolayers in parasite culture medium (DMEM [Gibco], 20% Medium 199 [Gibco] ...
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bioRxiv - Genetics 2022Quote: ... hiPSC-CMs were then transfected with siRNAs directed against PAX9 and EGR2 (ON-TARGETplus Human) using lipofectamine RNAi Max (ThermoFisher). Each siRNA was tested individually in 8-plicates ...