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Citations for Thermo Fisher :
6701 - 6750 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Molecular Biology 2022Quote: ... and 24 hours later cells were transfected with the Bluescript vector (empty vector control) and Human L1 plasmid (used in L1 retrotransposition assay) using Lipofectamine 2000 reagent (Invitrogen). Cells were harvested 36 hours after transfection ...
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bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...
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bioRxiv - Molecular Biology 2022Quote: ... 2×105 cells were transfected with 20 pmol of ON- TARGETplus Human HMG20A (10363) siRNA-SMART pool (Dharmacon) using Oligofectamine™ according to the manufacturer’s instructions (Invitrogen). Cells were cultured for 3 days before being harvested for follow-up experiments.
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bioRxiv - Neuroscience 2022Quote: ... human BDNF-biotin (Alomone labs; B-250-B) was conjugated to Quantum dots-605 (QD-605) (Thermo Fisher Scientific; Q10103MP). Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Bioengineering 2024Quote: Human BJ fibroblasts (ATCC CRL-2522) were cultured on standard cultureware in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco 11965-092) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Acquired spectral data files were searched against the human component of UniProtKB/SwissProt database using SEQUEST search engine in the Proteome Discoverer 1.4 (Thermo Scientific). The following parameters were applied ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Molecular Biology 2024Quote: ... the RAD54L2 cDNA was assembled from a partial cDNA clone encoding Met109 to Lys1467 was from the human ORFeome collection (Lamesch et al, 2007) and a synthetic DNA fragment encoding Met1 to Glu108 (Invitrogen). RAD54L2K311R was constructed by QuikChange site-directed mutagenesis with primers (5’-AGATCACTTGCAAAGTTCTCCCCAGACCCATGCTG-3’ and 5’-CAGCATGGGTCTGGGGAGAACTTTGCAAGTGATCT-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
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bioRxiv - Neuroscience 2024Quote: Organoid culture supernatants and lysates were analyzed for secreted proteins using single- and multiplexed Human ProcartaPlex™ Panel (Thermofisher Scientific), following previously documented procedures.37,38 The plates were read using the Luminex MAGPIX xMAP instrument and xPONENT® software ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Cell Biology 2024Quote: ... the mucosal layer was removed from an approximately 1 cm3 fresh section of human colonic tissue and washed twice with 1X Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher, 14040133). The mucosa was transferred to a 5-ml conical tube containing 0.5 ml crypt isolation buffer (2 mM EDTA ...
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bioRxiv - Cell Biology 2024Quote: ... The process entailed cloning the various human ACTL7B gene variants into the pENTR TOPO vectors using the pENTR d-Topo cloning kit (Invitrogen), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... Primary antibody incubation was carried out overnight at 4 °C with human-CD68 monoclonal antibody (cat. No. 14-0688-82, Invitrogen) and CD19 Rabbit polyclonal antibody (cat ...
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bioRxiv - Immunology 2024Quote: ... Bound mAb was detected using goat anti-human (H+L)-HRP (1:1000 dilution) (Thermo Fisher Scientific, Waltham, MA, USA). In the case of His-tag detection ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM GlutaMAXTM (4.5 g/L D-glucose) (Life Technologies #10-566-024) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... The Pro-Pol fusion HA-tagged (ProWTPolWT and ProMUTPolWT) plasmids were generated using an in-house human PGK promoter-driven lentiviral vector via Gateway cloning (Invitrogen). Plasmids expressing HuSaV (GI.2 ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated human Anti-CD9 antibody (MEM-61; catalog no. MA1-19485) and biotinylated human anti-CD81 antibody (M38; catalog no. ab239238) were purchased from Thermofisher Scientific (Stockholm ...
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bioRxiv - Neuroscience 2023Quote: ... Zebrafish Kcnc3a channel was labeled using a polyclonal antibody originally generated against the human Kcnc3 protein (ThermoFisher Scientific PA5-53714) at a concentration of 2.5 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: The 387 complex genetics sarcomas were analyzed on Human Genome U133 Plus 2.0 array (900466, Affymetrix, Santa Clara, CA, USA), according to the manufacturer’s procedures ...
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bioRxiv - Systems Biology 2023Quote: 1000-3000 IEQ human islets pellets were resuspended in 200 µL PBS and treated with 20 µL 10 mg/mL Rnase A (Invitrogen) and 20 µL Protein Kinase K (Qiagen ...
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bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: RT-qPCR with TaqMan primer/probes was used to determine relative expression levels of miR-146a compared to U18 (human) or sno251 (mouse) endogenous control genes (ThermoFisher, assay IDs ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... β-actin expression was measured using an anti-human β-actin horseradish peroxidase-conjugated mAb (1:25000, Life Technologies, USA) as the loading control ...
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bioRxiv - Microbiology 2023Quote: Type I RH parasites (ATCC PRA-319) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) and maintained in DMEM (GIBCO) supplemented with 2 mM glutamine ...
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bioRxiv - Microbiology 2023Quote: ... CVCL_1926) and HEK293T engineered to overexpress human ACE2 (BEI NR-52511, RRID: CVCL_A7UK) were maintained in DMEM (Gibco, 11965-092) supplemented with 10% FBS (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... a separate aliquot of each sample during each immunoblot was probed with a monoclonal mouse anti-human GAPDH antibody (1:500 dilution; #43700, Invitrogen) and a peroxidase-conjugated goat anti-mouse secondary antibody (1:5,000 dilution ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C in media with or without 0.5 ng/ml (1.0 unit/ml) human IFN-γ (ThermoFisher Scientific) at time points ranging from 5 minutes to 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... A 10% (w/v) solution of biomaterial was prepared by manually mixing the pulverized biomaterial in Human endothelial serum free media (HESFM; Gibco) supplemented with fetal bovine serum (2% v/v ...
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bioRxiv - Developmental Biology 2023Quote: A small piece of primary human or mouse tissue was resected and embedded in Fisher Healthcare Tissue-Plus Optimum Cutting Temperature (OCT, ThermoFisher). This was frozen at -80 0C ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: All acquired MS2 spectra were searched against a UniProt human database using Sequest within Proteome Discoverer 1.4 (Thermo Fisher Scientific). The search parameters were as follows ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then incubated with a 1:10,000 dilution of goat anti-human Fc HRP secondary antibody (Invitrogen, A18817) in PBST for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... PBMCs were labeled with 5 μM CFSE and activated with Dynabeads™ Human T-Activator CD3/CD28 (Thermo Fisher Scientific). To evaluate immunosuppressive potential ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Genetics 2023Quote: ... was extracted from skin fibroblasts derived from two different Welsh Mountain ponies that were overexpressing human SOX11 (as described above) using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Cancer Biology 2023Quote: ... of the target genes together with a human GAPDH endogenous control and the use of 7500 SDS software (Applied Biosystems). The primers used in this study were as follows ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The pENTR-hMCAK construct was generated by amplifying the coding sequence of human MCAK by PCR and inserting it into a pENTR entry clone using the pENTRTM/D-TOPO kit (ThermoFisher). The pFB-6HF2hMCAK construct was created from pFB-6HFRET2-DEST vector and pENTR-hMCAK constructs using Gateway® cloning technology (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... Clathrin Heavy Chain siRNA (Human CLTC, sequence: GGUUGCUCUUGUUACG, ID: s475) and Negative Control#1 siRNA Silencer Select were purchased from ThermoFisher Scientific.
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bioRxiv - Bioengineering 2023Quote: ... TJC4) was detected using a 1:500 dilution of goat anti-human Alexa Fluor 488 or Alexa Fluor 647 (Invitrogen). Secondary antibodies were incubated for 15 min at 4°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... MiniBAR-GFP sta-ble cell line was transfected with 25 nM of siRNAs targeting luciferase (5’-CGUACGCGGAAUACUUCGA-3’) or human Rab35 (5’-GCUCACGAAGAACAGUAAA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...