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Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Human ASB8 cDNA in pENTR221 vector was purchased as an Ultimate ORF clone (Thermo Fisher Scientific, clone ID IOH3117), PCR amplified and inserted into pGEX-4T3 (AmpR ...
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bioRxiv - Genetics 2024Quote: ... we obtained constructs which were codon-optimized for Drosophila and encoded the human protein from GeneARTTM (Thermo Fisher Scientific) (See Supplemental Text) ...
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bioRxiv - Cell Biology 2024Quote: A bead-based multiplex immunoassay system (Multiplex kit Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A, ThermoFisher, USA) was applied to measure the secretion of cytokines and chemokines by the Silk-Ovarioids samples into the spent culture media after 42 days of culture ...
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bioRxiv - Biochemistry 2024Quote: ... Cell lines ExpiCHO cells and Expi293F cells were obtained from ThermoFisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... Mouse C2C12 cells: C2C12 cells (ATCC) and HEK293A cells (Thermo Fisher Scientific) were cultured in DMEM with 4.5 g/L glucose and 584 mg/L L-glutamine (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Bovine or human TLR2 sequences of interest were cloned into pcDNA3.3 TOPO TA mammalian expression vector (Thermo Fisher Scientific, UK), and transfected into SEAP HEK cells using TurboFect transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The extraction and purification of human genomic DNA were performed using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific) and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: ... and the secondary antibodies including horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (#31439) and HRP-conjugated goat anti-human IgG (#31410) were purchased from Invitrogen. The secondary antibody for HRP-conjugated goat Anti-rabbit IgG (#12-348 ...
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bioRxiv - Genomics 2020Quote: Primary human keratinocytes were isolated from fresh surgically discarded neonatal foreskin and cultured in Keratinocyte-SFM (Life Technologies 17005-142) and Medium 154 (Life Technologies M-154-500) ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Systems Biology 2022Quote: ... RNA from human skin and wounds was reverse transcribed using TaqMan® Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific). Individual miRNA expression was then quantified using TaqMan® Advanced miRNA Assays (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Ambion™ Silencer™ Pre-Designed siRNA specific of human GLUT3 (SLC2A3) was purchased from Fisher Scientific (#10446914, Illkirch, France)
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bioRxiv - Neuroscience 2021Quote: DIV80 human iPSC-derived astrocytes were incubated with the calcium indicator Fluo-4 AM (1μM; ThermoFisher Scientific; Cat. No. F14201) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with the following primary antibodies: rabbit anti-Profilin 1 (Novus Biologicals, NB200-262, 1:10’000 for human lysates; Invitrogen, PA5- 17444 ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... The next day when cells were at 60–70% confluency and were transduced with 10 µl of lysate normalized to 3ng/µl per well of tau from rTg4510 mice or 5ng/µl per well of tau from human brains plus 1% Lipofectamine 2000 (Invitrogen) diluted in OptiMEM (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Bioengineering 2020Quote: ... R&D systems sheep anti-human CD31/PECAM-1 antibody (AF806) and Hoechst 33324 (H3570) were obtained from Thermo Fisher Scientific (Whaltam ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human astrocytes were grown on the bottom of transwell inserts (24-well, 0.1 um pore size, PET, Fisher Scientific) which were coated with poly-L-lysine (20 ug/mL) ...
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bioRxiv - Biochemistry 2021Quote: Codon optimized human SERT was assembled from synthetic DNA fragments (IDT) and inserted into the NheI-XhoI sites of pCEP4 (Invitrogen). The use of a synthetic gene ensures there are no sequence features ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were generated by RUCDR Infinite Biologics® from human primary lymphocytes using Sendai viral vectors (CytoTune™, ThermoFisher Scientific), as previously described (Moore JC et al ...
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bioRxiv - Cancer Biology 2021Quote: Isolated human PBMCs were seeded and grown in 6 well culture dish with hybridoma-(SFM) serum free medium (Cat #12045076, Gibco). PBMCs were treated with patient EVs under serum free conditions for 24 hrs ...
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bioRxiv - Cell Biology 2021Quote: Human foetuses were obtained following elective termination of pregnancy with full consent and stored overnight in Hibernate-A Medium (Gibco) at 4° C ...
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bioRxiv - Cell Biology 2021Quote: MS/MS spectra were searched against a Swiss-Prot human database containing 20,341 reviewed protein entries using Mascot algorithm (Matrix Science) via Proteome Discoverer 2.2 (PD, Thermo Fisher Scientific) and were processed as previously described (10) ...
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bioRxiv - Cell Biology 2021Quote: ... full length N-terminally 3xFLAG tagged human KNSTRN ORF was cloned into pTREx-DEST30 vector by serial Gateway recombinations (Invitrogen) according to the manual ...
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bioRxiv - Cell Biology 2021Quote: ... A probe mix containing 60 ng of each library probe and fluorescently labeled readout probe and 40 μg human COT1 DNA (Invitrogen) in 1.1 ml of hybridization buffer (20% dextran sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... cardiomyocytes were incubated with the secondary antibody diluted in a blocking buffer solution: goat anti-Human Alexa Fluor 647 (1:400; A-21445 - Invitrogen) and goat anti-Mouse 594 (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... HDL was labeled with Alexa568 by incubating 4.5 mg Human HDL in 90 μl 1 M sodium bicarbonate with Alexa Fluor 568 succinimidyl ester (A20003, Thermo Fisher) for 1 hr at room temperature (8:1 dye:estimated apoA1 mole ratio) ...
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bioRxiv - Cancer Biology 2020Quote: ... HFKs and iHFKs were grown and maintained in keratinocyte-serum-free media (KSFM) supplemented with human recombinant epidermal growth factor and bovine pituitary extract (Invitrogen). IMR-90s normal human diploid lung fibroblasts were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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bioRxiv - Cell Biology 2021Quote: Human islet and hESC-derived differentiated organoids (approximately 20 organoids or human islets per well) were respectively plated into a 96-well black plate (ThermoFisher) coated with 1% hESC-qualified Matrigel ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... human serum or oseltamivir were serially two-fold diluted in Dulbecco’s phosphate buffered saline with calcium and magnesium (ThermoFisher Scientific) containing 1% BSA (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies were diluted 1:100 in PBS-T as follows: single labeling: CD14 (mouse anti-human, 14-149-82, Invitrogen), CD56 (mouse anti-human ...
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bioRxiv - Microbiology 2021Quote: ... the bound antibody was detected with goat anti-human Ig (γ chain specific)-HRP conjugated secondary antibody (Thermo Fisher Scientific) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... and human fibroblasts (HF) clone EF-1-F (sourced from Doorbar lab, University of Cambridge) were grown in MEM (Gibco) supplemented with 10 % FBS ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500ng of a subset of ACE2 expression constructs (human, hamster, rabbit, pig, chicken, horseshoe bat) or mock transfected with an empty vector (pDISPLAY) in OptiMEM (Gibco) using TransIT-X2 transfection reagent (Mirus ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293T overexpressing the human ACE2 were kindly provided by Integral Molecular Company and maintained in DMEM with 1 μg/mL of puromycin (Invitrogen). TMPRSS2 human plasmid (Origene ...
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bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/mL of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells ...
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bioRxiv - Microbiology 2021Quote: ... and HCT 116 p53- /- (human, kind gift from Dr. Wilson) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplied with 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... HUVECs were cultured in a mixture of 199 medium and SFM (Human Endothelium Serum-Free Medium, GIBCO, cat. No. 11111044), with 10% of FBS (Biowest ...