Labshake search
Citations for Thermo Fisher :
6651 - 6700 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
-
bioRxiv - Immunology 2020Quote: ... Digested sera was probed with Rabbit anti-Human IgG-HRP H+L Secondary Antibody (ThermoFisher Scientific Catalog #A18903, 1:10,000), Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423 ...
-
bioRxiv - Cell Biology 2019Quote: IMR-90 primary human lung fibroblasts (ATCC #CCL-186) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco #12430-054) supplemented with penicillin and streptomycin (5000 U/mL and 5000 μg/mL ...
-
bioRxiv - Cancer Biology 2020Quote: ... Macrophages were obtained from the EpCAM− cells after 2 hours of adherence to the tissue culture dish and were cultured with 50 ng/ml human macrophage colony-stimulating factor (M-CSF, #CYT-308, ProSpec) in RPMI-1640 (Gibco) containing 20% fetal bovine serum (FBS ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... In order to generate the construct for inducible ZEB2 expression from the AAVS1 site the coding sequence of the human ZEB2 transcript variant 1 (NM_014795.3) was purchased as an ultimate ORF clone in the pENTRY221 (Thermo Scientific, #IOH53645). The ZEB2 ORF was amplified with primers ZEB2_IndOE_F & _R and tagged with GFP-Flag by Gibson assembly into the pT2-CAG-MCS-2A-Puro plasmid linearized by restriction digestion with EcoRI (NEB ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with pEGFP-human LA (EGFP-wt LA) and mutants after 3h from release of the nocodazole block using Lipofectamine 2000 (Invitrogen) in accordance with the manufacturer’s protocol at early passages having 80-85% confluency and were kept in culture for 24 hrs [67] ...
-
bioRxiv - Cell Biology 2021Quote: The VEGF-A concentration in the culture medium was analyzed using a VEGF-A Human ProcartaPlex Simplex Kit (Cat. no. EPX01A-10277-901, Invitrogen), specific for the detection of VEGF-A165 ...
-
bioRxiv - Biochemistry 2020Quote: ... serum reactivity to the Spike protein was measured using an HRP-conjugated goat anti-human IgG (H+L) (Invitrogen, 31410) at a 1:3000 dilution for 1 hour ...
-
bioRxiv - Cancer Biology 2020Quote: Surgically-resected human brain metastases and newly diagnosed glioblastomas were collected in neurobasal media A supplemented with 1x B27 (17504-044, Gibco), 1x N-2 (17502-048 ...
-
bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... fresh human pancreatic islets preparations were resuspended in CMRL culture medium (cat. 11-530-037, ThermoFisher Scientific, Waltham, MA, USA) supplemented with L-Glutamine 1% (cat.G7513-100ML) ...
-
bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with staining buffer and incubated in 0.01 μg/mL AF 488 secondary anti-human IgG (Invitrogen, A18821) solution for 30 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... IPSCs were maintained on human Laminin-521 (BioLamina, Sundbyger, Sweden) coating in essential 8 (E8) medium (DMEM/F12 supplemented with 15 mM Hepes (Gibco/Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: The 2019-nCoV receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen). The 2019-nCoV RBD (residues Arg319-Phe541 ...
-
bioRxiv - Immunology 2020Quote: ... Wells were washed three times with 1xDPBS followed by a one hour incubation with horse radish peroxidase-conjugated goat anti-human total Ig at a dilution of 1:6000 (Invitrogen). Wells were then washed four times with 1xDPBS followed by a 5-15 minute incubation with TMB substrate (SeraCare) ...
-
bioRxiv - Microbiology 2020Quote: ... and HRT-18G (ATCC CRL-11663; human colorectal adenocarcinoma) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco Life Technologies) supplemented with 8% heat-inactivated fetal bovine serum (HyClone ...
-
bioRxiv - Immunology 2020Quote: ... the cytokine levels were determined by Luminex xMAP technology using Human Custom ProcartaPlex Assay kit (Thermofisher, Cat PPX-04-MXRWEYU) in accordance with the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... on HEK-293T overexpressing the human ACE2 and maintained in the previously described media containing 1 mg/ml of geneticin (Invitrogen) to obtain TMPRSS2/ACE2 HEK-293T cells.
-
bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
-
bioRxiv - Immunology 2020Quote: ... the cells were fixed for 10 min at 4°C with 100 μL of the Fixation/Permeabilization solution and then washed and stained in 100 μL of the BD Perm/Wash Buffer containing anti-human polyclonal PPARγ antibody (PA3-821A, Invitrogen) at 1:25 for 1 hour at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: The catalytic domain of the human ST6Gal I (residue range 132-406) was expressed in a BL21(DE3) Escherichia coli strain (Invitrogen), using a polycistronic Ptac vector containing the CyDisCo system (Gąciarz et al ...
-
bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
-
bioRxiv - Cell Biology 2021Quote: To prepare the probe for hybridisation approximately 200-600ng of labelled probe DNA was ethanol precipitated with the 5 µg of sheared salmon sperm DNA and 5 µg of mouse or human Cot1 DNA (both from Invitrogen) and resuspended in hybridisation buffer (50% formamide ...
-
bioRxiv - Cell Biology 2021Quote: ... For spindle length and chromosome alignment analyses the following secondary antibodies were used at 1:500 dilution: Goat anti-Human IgG conjugated to Alexa Fluor 488 (A11013; Invitrogen), Goat anti-Mouse IgG conjugated to Alexa Fluor 647 (A21236 ...
-
bioRxiv - Cell Biology 2021Quote: ... falciparum cell lines were cultured in vitro in human erythrocytes (UK Blood and Transfusion Service) at 37°C in RPMI-1640 (Gibco) based media supplemented with 5% (w/v ...
-
bioRxiv - Cell Biology 2021Quote: ... fresh human pancreatic islets preparations were resuspended in CMRL culture medium (cat. 11-530-037, Fisher Scientific, Pittsburgh, PA, USA) supplemented with L-Glutamine 1% (cat ...
-
bioRxiv - Cancer Biology 2019Quote: ... Real-time quantitative RT-PCR (qRT-PCR) was performed to assess the expression of human PIK3CD (Hs00192399_m1) using the TaqMan gene expression assay (Applied Biosystems) while GAPDH was used as reference (Hs99999905_m1) ...
-
bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
-
bioRxiv - Microbiology 2022Quote: ... Plates were washed four times with washing buffer followed by incubation with HRP-conjugated anti-Human IgG secondary Abs (Invitrogen) (prepared in a diluted solution of blocking buffer [0.4% BSA] ...
-
bioRxiv - Immunology 2022Quote: ... Kits and murine and human serum CD40L was quantified using the commercially available soluble CD40L ELISA Kits (Thermo Fisher Scientific), following the manufacturer’s procedure.
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with corresponding secondary antibodies for 1 hr at room temperature: goat anti-human-Alexa-488 and goat anti-mouse-Alexa-488 (Invitrogen) for 293T internalization studies and goat anti-rabbit-Alexa-488 and goat anti-human-Alexa-555 (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... containing N-terminal ten histidine tag (His-tag) and human rhinovirus 3C (HRV 3C) protease site was amplified by PCR and cloned into the pFastBac vector (Invitrogen) using seamless cloning method ...
-
bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...
-
bioRxiv - Neuroscience 2022Quote: ... Affymetrix Human Transcriptome Array 2.0 ST arrays were hybridized according Affymetrix recommendations using the Ambion WT protocol (Life technologies, France) and Affymetrix labelling and hybridization kits ...
-
bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from rat glomeruli tissue and human podocyte samples using the mirVanaTM Isolation Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags ...
-
bioRxiv - Cell Biology 2022Quote: Cryo-preserved primary human hepatocytes (PHH) from single donors (BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; and Thermo Fisher Scientific) were used for all in vitro experiments ...
-
bioRxiv - Immunology 2022Quote: ... in culture media or stimulated for 48h with α-CD3/CD28 coated beads (Dynabeads Human T-Activator, Thermo Fisher scientific) in a 1:1 ratio (beads to cells) ...
-
bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
-
bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
-
bioRxiv - Cell Biology 2022Quote: Toxoplasma gondii RH∆hxgprt strain was maintained by growth in confluent primary human foreskin fibroblasts (HFFs) in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
-
The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
-
Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... were designed and used to create three different human-yeast hybrid genes by PCR (AccuPrime Pfx DNA polymerase from Invitrogen). The primers also add the attB1 and attB2 sites at the 5’ and 3’ ends of the PCR for cloning in pDONR221 entry clones using the BP cloning strategy ...
-
bioRxiv - Immunology 2022Quote: ... Sorted Treg cells from HD and IPEX patients were stimulated with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
-
bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
-
bioRxiv - Genomics 2022Quote: Purified human and bacterial DNA were quantified for concentration with the Qubit dsDNA HS assay (Life Technologies, Q33231, MA, USA), and pooled by adding 94% of human DNA ...
-
bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: Telomerase-transduced Human Foreskin Fibroblasts (HFFs) and MRC5 lung fibroblasts were cultured in Dulbecco’s modified Eagle serum (DMEM; Invitrogen #11965118) supplemented with 10% (v/v ...