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Citations for Thermo Fisher :
6601 - 6650 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The plate was then washed and incubated with horseradish peroxidase-conjugated anti- human IgG (InvitrogenTM, ThermoFisher Scientific, Waltham, MA) at a dilution of 1:2000 for 2h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pooled RNA were hybridized to Affymetrix Gene Chip Human Gene 1.0 ST arrays (Thermo Fisher Scientific, Meerbusch, Germany, 901086). cDNA syntheses ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Cancer Biology 2024Quote: ... The Macrophage panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:50, 48-3697-82, Invitrogen) and anti-mouse Ki67-PE/Cyanine7 (1:150 ...
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...
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bioRxiv - Neuroscience 2024Quote: ... Human iPSCs were cultured as colonies in feeder-free conditions using Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES-qualified (Corning ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: RNA extracted from human left ventricular tissue was digested with DNase I (Invitrogen DNA-free DNA removal kit AM1906). 20 ng digested RNA was depleted of rRNA (NEBNext rRNA depletion kit E6350L) ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a secondary Alexa Fluor 647 conjugated goat anti-human IgG (H+L) antibody (Invitrogen #A-21445; 10 μg/mL) was used.
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Promutagens were first activated by incubating in equal volumes with a 2-fold human microsomal (ThermoFisher Cat. No. HMMCPL) reaction mixture containing 1mg/mL microsomes ...
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bioRxiv - Molecular Biology 2024Quote: ... All human L1 plasmids contain the indicated fragments of L1.3 DNA (a retrotransposition-competent human L1, GenBank accession no. L19088 (32)) cloned into pCEP4 (Invitrogen) unless indicated otherwise ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Physiology 2024Quote: ... The PCR amplification of human Piezo1 was performed using a Veriti Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA) and ExTaq (Takara ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fragments of human L1 ORF1 and ORF2 from the EF06R plasmid were amplified by PCR and cloned into pcDNA3.1(+) (#V79020, Invitrogen) with the puromycin resistant gene split by an intron ...
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bioRxiv - Cell Biology 2024Quote: ... Human precision cut lung slices (PCLS) were purchased from IIVS and were grown in DMEM/F-12 media (Gibco) supplemented with antibiotics and antimycotic reagents.
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bioRxiv - Immunology 2024Quote: ... The following fluorochrome-labeled anti-human Abs were used for surface staining: PerCP/Cy5.5-CD19 (Invitrogen, #45-0198-42), APC-CD27 (eBioscience ...
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Interaction between gene expression and morphokinetic parameters in undisturbed human embryo culturebioRxiv - Developmental Biology 2024Quote: ... Labelled PolyAcDNA was then hybridised to the GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix, SantaClara, CA, USA) that covers over 47,000 transcripts and have been commonly used for human transcriptome analysis ...
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bioRxiv - Microbiology 2024Quote: ... Alexa fluor 647-conjugated goat anti-human IgG was used as the secondary antibody (Invitrogen, A-21445, 1:500). For phospholipase C (PLC ...
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-adsorbed goat anti-human IgM (heavy chain specific) (Invitrogen, A-21215) before washing as above and fixing in PBS/0.5% paraformaldehyde ...
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bioRxiv - Microbiology 2024Quote: ... the S protein labelling was visualized with an Alexa-488-conjugated goat anti-human antibody (ThermoFisher, cat. No.: A11013), while for the N protein labeling an Alexa-488-conjugated goat anti-rabbit secondary antibody (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... All human L1 plasmids contain the indicated fragments of L1.3 DNA (a retrotransposition-competent human L1, GenBank accession no. L19088 (31)) cloned into the pCEP4 (Invitrogen) episomal vector unless indicated otherwise ...
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bioRxiv - Neuroscience 2024Quote: Cryopreserved GW18-19 human MGE tissue stocks were thawed in warm Leibovitz’s L-15 Medium (Thermo Fisher Scientific # 11415064). Following thawing ...
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bioRxiv - Neuroscience 2024Quote: ... MS/MS spectra were searched against the Uniprot human database (downloaded on 04/2015) with Proteome Discoverer 2.1.1.21 (Thermo Fisher Scientific). Variable and static modifications included methionine oxidation ...
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bioRxiv - Cell Biology 2024Quote: ... The amount of albumin and A1AT in the medium was then determined using Albumin Human ELISA Kit (Invitrogen, EHALB) and Human alpha-1-Antitrypsin ELISA Kit (AssayPro ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from mouse or human brain tissues using TRIzol™ reagents (Thermo Fisher Scientific, Cat# 15596026) following the manufacturer’s instructions and appropriately stored at -80°C until further use ...
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bioRxiv - Microbiology 2024Quote: ... 50 µL of goat anti-Human IgG Fc HRP antibody (0.5 µg/mL, Cat: A18829, Thermo Fisher Scientific, USA) with blocking buffer was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... Primary human embryonic lung fibroblasts (HEL 299; PHE, 87042207) were grown in Minimal Eagle Medium (MEM; Invitrogen, 21090-22) supplemented with 1% L-glutamine (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The basal BM2 medium for murine and human CRC tumor-organoids culture consisted of Advanced Dulbecco’s Modified Eagle Medium (DMEM)/F12 medium (Gibco) supplemented with 10 mM N’-2-Hydroxyethylpiperazine-N’-2 ethanesulphonic acid (HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... Levels of mRNA were quantified using a TaqMan Human Molecular Mechanisms of Cancer Array (Applied Biosystems, Foster City, CA) that utilizes real-time qPCR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The 3xHA-NTSR1 plasmid consists of N-terminal 3xHA-tagged wild-type human NTSR1 cloned into the pcDNA3.1(+) vector (Invitrogen, Thermo Fisher Scientific Corporation ...
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bioRxiv - Microbiology 2024Quote: ... falciparum NF54 asexual stage parasites were cultured under standard conditions in RPMI-HEPES medium at 4% hematocrit (human erythrocytes of O+ group) and 0.5% (w/v) AlbumaxTM (Invitrogen) and kept at 37°C in a gas mixture of 5.0% oxygen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with Gibco™ Human Melanocyte Growth Supplement-2 (HMGS-2; Gibco™, US-referred to as HMGS2 media) or Gibco™ Human Melanocyte Growth Supplement (HMGS ...
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bioRxiv - Immunology 2024Quote: ... The presence of EVs was determined flow cytometrically using Exosome-Human CD63 Isolation/Detection Reagent according to manufacturer’s instructions (Invitrogen) and the absence of contaminating soluble proteins using the Qubit Protein Assay (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The detection antibody used was horseradish peroxidase (HRP)–conjugated goat anti-human IgG (H+L) (ThermoFisher, cat# SA5-10283) at a 1:2000 dilution ...
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bioRxiv - Immunology 2024Quote: IgA or IgG were depleted from human plasma samples using CaptureSelect™ IgA or IgG Affinity Matrix (Thermo Fisher). The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore) ...
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bioRxiv - Developmental Biology 2024Quote: ... siRNAs were transfected into human chondrocytes obtained from articular cartilage using Lipofectamine™ RNAiMAX Transfection Reagent (Thermo Fisher Scientific). After treatment with siRNA for 1 week ...
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bioRxiv - Immunology 2024Quote: ... After being stimulated with anti-CD3/anti-CD28 (Cdmix) (Dynabeads™ Human T-Activator CD3/28, Gibco, Vilnius, Lithuania), lymphocytes were cultured with 50 µg/ml exosomes of IFN-γ primed (IFN+Exo ...
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bioRxiv - Immunology 2024Quote: ... Catalog Number 00-4976) or F(ab’)2-anti-Human IgM/IgG (10μg/ml; Invitrogen, Catalog Number 16-5099086) + IL4 (20ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Both recombinant proteins were pre-clustered with 10 µg/mL of an anti-human IgG antibody (Thermo Fisher, U.S.A.). Cortical single cells were fixated with 4% PFA/1x PBS for 10 min.
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bioRxiv - Genomics 2024Quote: ... Human lung tissue was filled with 3 % of low gelling temperature agarose in DMEM/F-12 (Thermo Scientific, USA) with phenol red supplemented with 0.1 % FCS ...
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bioRxiv - Bioengineering 2024Quote: Cryopreserved hiPSCs were thawed and plated into 6-well culture plates coated with 1.1 µg/cm2 recombinant human laminin-521 (rhLN-521, A29248, Gibco) in 2 ml mTeSR Plus medium (100-0274 ...
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bioRxiv - Cancer Biology 2024Quote: ... Data were processed by database searching against Human Uniprot Proteome database using Proteome Discoverer 2.2 software (Thermo Fisher Scientific). Precursor and fragment mass tolerance were set at 7 ppm and 0.6 Da respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... commercially available pool (n=23) of total RNA isolated from healthy human brains was purchased (Ambion, cat. No# AM6050) and utilized as control ...
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bioRxiv - Cell Biology 2024Quote: Human islets were incubated in neuronal medium containing 3 μM Bodipy 558/568 C12 (Bodipy C12, ThermoFisher, Waltham, MA) with vehicle (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... extracts from BMDMs or human visceral adipose tissue (VAT) were prepared using M-PER mammalian extraction buffer (Thermo Scientific) containing Halt phosphatase inhibitor cocktail (1:50 ...
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bioRxiv - Biochemistry 2024Quote: The human BEST1 protein (spanning amino acids 1-398) was expressed in Pichia pastoris using the pPICZ vector (Invitrogen) with a C-terminal green fluorescent protein (GFP ...
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bioRxiv - Cancer Biology 2024Quote: ... Human PDO#2 line was grown in DMEM/F12 [+] L-Glutamine [+] 2.438 g/L sodium bicarbonate (Gibco, 11320-033) supplemented with 1X Penicillin/Streptomycin (Corning ...