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Citations for Thermo Fisher :
6501 - 6550 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The plate was then washed and incubated with horseradish peroxidase-conjugated anti- human IgG (InvitrogenTM, ThermoFisher Scientific, Waltham, MA) at a dilution of 1:2000 for 2h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pooled RNA were hybridized to Affymetrix Gene Chip Human Gene 1.0 ST arrays (Thermo Fisher Scientific, Meerbusch, Germany, 901086). cDNA syntheses ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... Human precision cut lung slices (PCLS) were purchased from IIVS and were grown in DMEM/F-12 media (Gibco) supplemented with antibiotics and antimycotic reagents.
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bioRxiv - Immunology 2024Quote: ... The following fluorochrome-labeled anti-human Abs were used for surface staining: PerCP/Cy5.5-CD19 (Invitrogen, #45-0198-42), APC-CD27 (eBioscience ...
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Interaction between gene expression and morphokinetic parameters in undisturbed human embryo culturebioRxiv - Developmental Biology 2024Quote: ... Labelled PolyAcDNA was then hybridised to the GeneChip® Human Genome U133 Plus 2.0 Array (Affymetrix, SantaClara, CA, USA) that covers over 47,000 transcripts and have been commonly used for human transcriptome analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... The Macrophage panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:50, 48-3697-82, Invitrogen) and anti-mouse Ki67-PE/Cyanine7 (1:150 ...
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...
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bioRxiv - Neuroscience 2024Quote: ... Human iPSCs were cultured as colonies in feeder-free conditions using Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES-qualified (Corning ...
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bioRxiv - Biochemistry 2024Quote: Protein in mouse and human wound fluid samples were quantified with the BCA Protein Assay kit (Thermo Fisher Pierce) using the 96-well plate protocol recommended by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: RNA extracted from human left ventricular tissue was digested with DNase I (Invitrogen DNA-free DNA removal kit AM1906). 20 ng digested RNA was depleted of rRNA (NEBNext rRNA depletion kit E6350L) ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a secondary Alexa Fluor 647 conjugated goat anti-human IgG (H+L) antibody (Invitrogen #A-21445; 10 μg/mL) was used.
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bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Promutagens were first activated by incubating in equal volumes with a 2-fold human microsomal (ThermoFisher Cat. No. HMMCPL) reaction mixture containing 1mg/mL microsomes ...
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bioRxiv - Molecular Biology 2024Quote: ... All human L1 plasmids contain the indicated fragments of L1.3 DNA (a retrotransposition-competent human L1, GenBank accession no. L19088 (32)) cloned into pCEP4 (Invitrogen) unless indicated otherwise ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Immunology 2024Quote: Primary human PBMCs and CAR T derivatives thereof were cultured in RPMI 1640 (Gibco, Life Technologies, Carlsbad, CA, USA) with 10% FCS ...
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bioRxiv - Physiology 2024Quote: ... The PCR amplification of human Piezo1 was performed using a Veriti Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA) and ExTaq (Takara ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fragments of human L1 ORF1 and ORF2 from the EF06R plasmid were amplified by PCR and cloned into pcDNA3.1(+) (#V79020, Invitrogen) with the puromycin resistant gene split by an intron ...
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bioRxiv - Physiology 2021Quote: ... Mouse C2C12 cells: C2C12 cells (ATCC) and HEK293A cells (Thermo Fisher Scientific) were cultured in DMEM with 4.5 g/L glucose and 584 mg/L L-glutamine (Corning) ...
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bioRxiv - Cell Biology 2020Quote: ... Each labeled sample was assayed with the Affymetrix PrimeView human gene expression array read with an Affymetrix GeneChip Scanner 3000 (Affymetrix/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... cells were subsequently pulsed at 370C for different time points with 25µg/ml Alexa flour-488 or- 568-conjugated human transferrin (Invitrogen, ThermoFisher Scientific) reconstituted in starvation medium and clarified by centrifugation at 15,000 × gmax at 40C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Bovine or human TLR2 sequences of interest were cloned into pcDNA3.3 TOPO TA mammalian expression vector (Thermo Fisher Scientific, UK), and transfected into SEAP HEK cells using TurboFect transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... The extraction and purification of human genomic DNA were performed using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific) and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2020Quote: ... and the secondary antibodies including horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (#31439) and HRP-conjugated goat anti-human IgG (#31410) were purchased from Invitrogen. The secondary antibody for HRP-conjugated goat Anti-rabbit IgG (#12-348 ...
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bioRxiv - Genomics 2020Quote: Primary human keratinocytes were isolated from fresh surgically discarded neonatal foreskin and cultured in Keratinocyte-SFM (Life Technologies 17005-142) and Medium 154 (Life Technologies M-154-500) ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Systems Biology 2022Quote: ... RNA from human skin and wounds was reverse transcribed using TaqMan® Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific). Individual miRNA expression was then quantified using TaqMan® Advanced miRNA Assays (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Ambion™ Silencer™ Pre-Designed siRNA specific of human GLUT3 (SLC2A3) was purchased from Fisher Scientific (#10446914, Illkirch, France)
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bioRxiv - Neuroscience 2021Quote: DIV80 human iPSC-derived astrocytes were incubated with the calcium indicator Fluo-4 AM (1μM; ThermoFisher Scientific; Cat. No. F14201) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were then incubated with the following primary antibodies: rabbit anti-Profilin 1 (Novus Biologicals, NB200-262, 1:10’000 for human lysates; Invitrogen, PA5- 17444 ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... The next day when cells were at 60–70% confluency and were transduced with 10 µl of lysate normalized to 3ng/µl per well of tau from rTg4510 mice or 5ng/µl per well of tau from human brains plus 1% Lipofectamine 2000 (Invitrogen) diluted in OptiMEM (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2019Quote: Human genital ridges were dissected in PBS and separated from the mesonephros followed by dissociation with TrypLE Express (Life Technologies) at 37°C for 20-40 minutes (depending on the tissue size ...
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bioRxiv - Cancer Biology 2019Quote: ... were used to retro-transcribe 1 μg of total RNA from Human Liver Total RNA (ThermoFisher Scientific, Waltham, MA, USA). The reverse transcriptase reaction for the viral pellet was carried out with random primers using a commercial kit (EasyScript cDNA Synthesis kit ...
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bioRxiv - Neuroscience 2019Quote: ... or 1 µg of pCMV-SPORT6.FBXL3 and 40 nM ON-TARGETplus human CUL1 (8454) siRNA SMARTpool (Dharmacon, L-004086) using the Neon transfection system (Invitrogen) following the manufacturer instructions and 1400 V ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting supernatant was decanted and the concentration of secreted IL6 was measured by the human IL6 ELISA kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: The pCMV-c-JUN-MYC plasmid was generated by cloning the human c-JUN coding sequence in the pCMV-MYC plasmid (ThermoFisher). Primers used for generation of the construct are shown in supplementary table S5 ...
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bioRxiv - Genetics 2019Quote: ... Extracted DNA samples were quantified following the Quantifiler Duo Human DNA Quantification Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) as recommended by the manufacturer in 7500 Real-time PCR System (Thermo Fisher Scientific Company ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Bioengineering 2020Quote: ... R&D systems sheep anti-human CD31/PECAM-1 antibody (AF806) and Hoechst 33324 (H3570) were obtained from Thermo Fisher Scientific (Whaltam ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Bioengineering 2021Quote: ... Primary human astrocytes were grown on the bottom of transwell inserts (24-well, 0.1 um pore size, PET, Fisher Scientific) which were coated with poly-L-lysine (20 ug/mL) ...
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bioRxiv - Biochemistry 2021Quote: Codon optimized human SERT was assembled from synthetic DNA fragments (IDT) and inserted into the NheI-XhoI sites of pCEP4 (Invitrogen). The use of a synthetic gene ensures there are no sequence features ...
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bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...