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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Genomics 2023Quote: ... cDNAs from the human KZFPs were codon-optimized and synthesized using the GeneArt service from ThermoFisher (former Life Technologies). Sequences were cloned into the doxycycline inducible expression vector pTRE-3HA which yields a C-terminally tagged proteins ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Bioengineering 2023Quote: ... The human dermal fibroblasts were cultured in a reconstituted Complete medium composed of DMEM medium (Dulbecco’s Modified Eagle Medium Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The human embryonic kidney 293T/17cells (ATCC, CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (Gibco Life Technologies) containing 10%FCS ...
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bioRxiv - Cancer Biology 2023Quote: ... pooled RNA were hybridized to Affymetrix Gene Chip Human Gene 1.0 ST arrays (Thermo Fisher Scientific, Meerbusch, Germany, 901086). cDNA syntheses ...
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bioRxiv - Cancer Biology 2023Quote: ... was used to prepare samples and to facilitate hybridization to the human Clariom™ D arrays (Thermo Fisher Scientific). Transcriptome Analysis Console (TAC ...
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bioRxiv - Microbiology 2023Quote: ... the human microbiome database was used as described by Li et al.30 and analyzed using Proteome Discoverer 2.5 (Thermofisher). For subsequent data analysis ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were co-transfected with the R278C-sgRNA-pCCCplasmid and the ssODN donor template using Lipofectamine 3000 (ThermoFisher). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... custom micropatterned glass coverslips presenting 500 µm groWTh areas (CYTOOCHIPS Arena 500A) were covered with 800µL of 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... hESCs were grown in glass surfaces previously coated with 10μg/ml recombinant human laminin 521 (BioLamina) diluted in PBS+/+(Gibco) for 3h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The human embryonic kidney 293T/17cells (ATCC, CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (Gibco Life Technologies) containing 10%FCS ...
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bioRxiv - Systems Biology 2023Quote: ... Thawed Primary human hepatocytes were recovered in Cryopreserved Hepatocyte Recovery Medium (Cat. CM700, Thermo Fisher Scientific, Waltham, MA, USA), and re-suspended in ice-cold HBSS prior to surgery ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary antibody used was R-Phycoerythrin labelled Goat anti-Human IgG Fc (eBioscienceTM; 12-4998-82, Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum and activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) at a 1:1 bead:cell ratio according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human neutrophils were used directly out of thaw and cultured on poly-l-lysine (15 µg/mL, Thermofisher) coated tissue culture dishes ...
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bioRxiv - Cancer Biology 2023Quote: ... and for human biopsies (see Fig. S1B and C) goat anti-ZO-1 (Thermo Fisher; PA5-19090; 1:200) and anti-laminin primary antibodies were used ...
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bioRxiv - Bioengineering 2023Quote: ... Two different lots (lot 1355434 and 1767629) of human dermal neonatal foreskin fibroblasts (hDFn) were purchased from Thermo Fisher Scientific (cat ...
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bioRxiv - Microbiology 2023Quote: Enriched neutrophils were resuspended at 1×106 cells/mL in RPMI with 20 ng/mL GM-CSF (R&D or STEMCELL) (for murine neutrophils) or RPMI with 2% FBS (for human neutrophils) with 1μM Sytox Green (Invitrogen) and plated in 96-well plates at 200,000 neutrophils per well ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were grown to log phase in the presence of 1:200 pooled normal human serum (Thermo Fisher Scientific) to opsonize bacteria ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA encoding full-length human CLASP2α was purchased from Dharmacon (Accession: BC140778.1) and subcloned into a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag ...
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bioRxiv - Cell Biology 2022Quote: ... Endometrial ADGR gene expression in these 21 samples was determined using the high-density human genome (HG) U133 Plus 2.0 Arrays (Affymetrix), containing 42,203 genes and 12,397 expressed sequence tags (ESTs) ...
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bioRxiv - Genomics 2023Quote: ... in condΔEXP168 were maintained in 3% hematocrit in human O+ blood in RPMI 1640 supplemented with 5% Albumax-II and 50µg/ml hypoxanthine (Gibco), 25mM HEPES (Sigma ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Immunology 2023Quote: ... the blot was labelled with horse-radish peroxidase (HRP) conjugated goat anti-human IgG antibody (Invitrogen, catalog number 31410) using a 1:3333 dilution in TBS-T ...
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bioRxiv - Immunology 2023Quote: ... the blot was labelled with horse-radish peroxidase (HRP) conjugated goat anti-human IgG antibody (Invitrogen, catalog number 31410) using a 1:3333 dilution in TBS-T ...
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bioRxiv - Cancer Biology 2022Quote: ... Each pool was labeled and hybridized to Affymetrix® Human Gene 2.1 ST according to the manufacturer instructions (Affymetrix). Quality control and normalization of Affymetrix.CEL files using the Robust Multi-array Average (RMA ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Genomics 2023Quote: ... falciparum clone II313 was maintained in human A+ erythrocytes at 37 °C in RPMI 1640 medium containing AlbumaxII (Invitrogen) supplemented with 2 mM L-glutamine ...
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bioRxiv - Developmental Biology 2023Quote: ... and/or ALK1 (SMARTpool ON-TARGETplus Human ACVRL1 siRNA, Dharmacon L-005302-02-0005) using Lipofectamine 3000 (ThermoFisher L3000015) according to manufacturer directions ...
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bioRxiv - Cell Biology 2023Quote: ... after which they were washed with 1% human albumin in PBS and stained with propidium iodide (Molecular Probes #P3566) for assessing viability ...
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bioRxiv - Cell Biology 2023Quote: ... The digested tissue was mixed with an equal volume of 1% human albumin (CSL Behring) in DPBS −/− (Gibco #14190094) to stop the lysis ...
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bioRxiv - Microbiology 2023Quote: ... Cat#40590-D001) and anti-human AF488 (1:1000 with final concentration of 2μg/ml; ThermoFisher Cat#A-11013) for secondary conjugation ...
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bioRxiv - Neuroscience 2023Quote: ... Amyloid Aβ40 and Aβ42 in the hippocampus and striatum and D2R in the striatum were quantified by ELISA with the following kits: Aβ40 human ELISA kit (Thermofisher); Aβ42 human ultrasensitive ELISA Kit (Thermofisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies) with Lipofectamine 2000 according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Genetics 2024Quote: ... 3% human serum (heat-inactivated from Sigma-Aldrich, St. Louis, MO, USA or Thermo Fisher Scientific, Waltham, MA, USA), 2% human plasma (isolated from umbilical cord blood provided by Stanford Binns Cord Blood Program) ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: MS raw data were searched against the UniProtKB human sequence data base (92,954 entries) and TMT 10plex quantitation performed using Proteome Discoverer software (v 2.3; Thermo Scientific). Search parameters were set to include carbamidomethyl (C ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then treated with human serum containing phospholipase D (Australian Red Cross Lifeblood) or 0.25% (w/v) Albumax II (Gibco) and incubated for 3 hours at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and separately two Silencer Select siRNAs targeting human Myosin ID (4392420; ID s9203 and s9204; Invitrogen, Thermo Fisher Scientific) at a final concentration of 0.01 uM using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Tg tachyzoites were maintained in vitro by serial passage on confluent monolayer of human foreskin fibroblasts (HFF, ATCC SCRC-1041) in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 1% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... DRG expression levels were determined from total RNA extracts measured using Affymetrix’ Human Transcriptome Array 2.0 (Affymetrix, Santa Clara), scanned with Affymetrix’ GeneChip 3000 G7 Instrument System ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ concentrations in three separate fractions of brain homogenates were determined by Invitrogen™ Amyloid β 42 and 40 Human ELISA kits (Cat.-No.: KHB3441 and KHB3481, respectively; both from Thermo Fisher Scientific). Results were normalized on the basis of the sample’s protein concentration.
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: All parasite lines were maintained in human foreskin fibroblasts (HFFs; ATCC) in Minimal Essential Media-α (MEM-α; Gibco) supplemented with 7% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: The gene sequences of human MCHR1(residues 70-405) and MCHR2(residues 1-331, with L2566.40Y mutation) was subcloned into pFastBac vector (Invitrogen) with an N-terminal haemagglutinin signal peptide (HA ...
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bioRxiv - Microbiology 2023Quote: Mouse fecal pellet and human stool sample data analysis was performed using FreeStyle software (version 1.8 SP2, Thermo Scientific). Metabolite identification was established by matching accurate mass ...
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bioRxiv - Biophysics 2023Quote: ... The plate was then washed and incubated with horseradish peroxidase-conjugated anti- human IgG (InvitrogenTM, ThermoFisher Scientific, Waltham, MA) at a dilution of 1:2000 for 2h at room temperature ...