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Citations for Thermo Fisher :
6451 - 6500 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and human T lymphocytes, Jurkat J16 (Adult lymphoblastic leukemia ACC-282, DSMZ) were cultured in RPMI-1640 (Thermo Fisher) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2022Quote: ... falciparum erythrocytic stages were cultured in human erythrocytes (National Blood Transfusion Service, UK) and RPMI 1640 medium (Life Technologies) supplemented with 0.5% Albumax type II (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mice were bled and total IgG (mouse and human) was pulled down using protein A beads (ThermoFisher, Catalog # 20333). An in–house sandwich ELISA was used to measure human IgG (control IgG or MS-Hu6 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: 1 mL of human plasma was incubated with 0.5 mL of Invitrogen total exosome isolation reagent (Thermo Fisher Scientific) overnight at 4°C and the day after was centrifuged at 10,000 x g for 1h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: cDNA libraries were constructed using Ion Ampliseq Transcriptome Human Gene Expression Kit from Thermo Fisher (MA, USA, Cat# A26325) according to manufacturer’s recommended protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Rat ASC were grown in the same medium than human ASC supplemented with 10% of fetal calf serum (Thermofisher). To manufacture the ASC-enriched gelatin patch ...
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bioRxiv - Neuroscience 2021Quote: ... PLA on human post-mortem paraffin-embedded tissue was performed on sections mounted on superfrost plus slides (Thermo Scientific). 6 μm-thick human caudate-putamen sections were deparaffinized in xylene ...
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bioRxiv - Molecular Biology 2021Quote: Affymetrix Human Exon 1.0 ST arrays were hybridized by GenoSplice technology (www.genosplice.com) according the Ambion WT protocol (Life Technologies, France) and Affymetrix (Santa Clara ...
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bioRxiv - Microbiology 2020Quote: ... Meso Scale Discovery (MSD)) were coated with mouse anti-human IgG antibody (CH2 domain, cat# MA5-16929, ThermoFisher Scientific) at 2 μg/mL in 1X PBS (50μL/well) ...
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bioRxiv - Microbiology 2021Quote: Human foreskin fibroblasts (HFFs) were seeded in 96 wells plates and grown to confluency in DMEM medium (ThermoFisher 11960044) at 37°C in 5% CO (26) ...
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bioRxiv - Genetics 2021Quote: ... 10 μg of total RNA was depleted of rRNAs by using the Human/Mouse RiboMinus Transcriptome Isolation Kit (Invitrogen). Ribosomal RNA depletion was confirmed by an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... The slides were washed and incubated for 2 h with secondary antibodies (Alexa 488 anti-Human IgG Thermo Fisher - #A11013 and Alexa Fluor 555 Anti-Mouse IgG #A21422) ...
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bioRxiv - Neuroscience 2021Quote: Human ESCs and iPSCs were maintained on plates coated with geltrex (life technologies, A1413301) in StemFlex media (Gibco, A3349401) and passaged with accutase (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-actinin-1-M-sstFRET containing the sstFRET module inserted between 300aa and 301aa within the first spectrin repeat of human alpha-actinin-1 (P12814.2) was in the neomyocin selectable pcDNA3.1 expression vector (Invitrogen/ThermoFisher). Actinin-C-sstFRET containing the sstFRET module added to the C-terminus of human alpha-actinin-1 (P12814.2 ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgG labeled with FITC (Chemicon) or goat anti-rabbit IgG labeled with Alexa 488 (Thermo Scientific) was used diluted at 4 μg/mL in PBS and incubated for 30 min with 5 μg/mL 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: cDNAs encoding human full-length of CREB3L3 (NM_032607.2) and green fluorescent protein were cloned into pENTR4 vectors (Life Technologies). In addition ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Neuroscience 2020Quote: The quantitation of soluble human Aβ1-42 in hippocampus extracts was determined with a sandwich antibody ELISA kit (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Neuroscience 2022Quote: ... APOE levels were similarly processed and quantified from iMGL conditioned media using Apolipoprotein E Human ELISA kit (Invitrogen; #EHAPOE).
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bioRxiv - Neuroscience 2022Quote: A 448 nt fragment of human SYN1 promoter was PCR amplified with Phusion high fidelity DNA polymerase (Thermo Scientific) and the following primers ...
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bioRxiv - Immunology 2022Quote: ... The detection antibody used was horseradish peroxidase (HRP)–conjugated goat anti-human IgG (H+L) (ThermoFisher, cat# SA5-10283) at a 1:2000 dilution ...
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bioRxiv - Immunology 2022Quote: Fn purified from human plasma (sigma) or monoclonal antibodies were fluorescently conjugated using AlexaFluor 488 and 647 Ester (Invitrogen). The conjugation and purification were done according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: Human U937 macrophages (ATCC, Cat. No. CRL-1593.2™) were maintained in RPMI (Thermo Fisher Scientific, Cat. No. 11875093) supplemented with 10% FBS (RMBIO ...
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bioRxiv - Cell Biology 2022Quote: The cDNA encoding full-length human CLASP2a was purchased from Dharmacon (Accession: BC140778.1) and subcloned into: (i) a pFastBacHT vector (Invitrogen) containing an N-terminal 6xHis-tag and ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Immunology 2020Quote: Whole genome transcript analysis was performed on 200 ng of total RNA using the Affymetrix Human Gene ST version 2.0 Array (Affymetrix). Genome-wide DNA methylation was profiled using the Illumina EPIC platform (Illumina ...
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bioRxiv - Physiology 2020Quote: Cryo-preserved primary human hepatocytes from single donors (PHH; BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; Thermo Fisher Scientific) and the human HCC cell line HuH7 (ATCC ...
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bioRxiv - Cell Biology 2020Quote: ... For RHBDL4 and OST4 depletion 50 pmol (RHBDL4) or 25 pmol (OST4) ON-TARGETplus SMARTpool human siRNA (Dharmacon) and 2.5 μl RNAimax (Invitrogen) transfection reagent per well of a 12-well plate were used ...
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bioRxiv - Cancer Biology 2020Quote: Copy number analysis was derived from profiling of total DNA on the Affymetrix genome-wide human SNP Array 6.0 chip using instrumentation and protocols from Affymetrix. The raw CEL files were returned and the QC steps were performed using Affymetrix Genotyping Console ...
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bioRxiv - Genetics 2021Quote: ... with 12.5 pmol of either non-targeting negative control siRNA showing no homology to the human transcriptome (#4457287, Ambion), or two different siRNAs targeting OAS1 (siRNA-1 or −2 ...
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bioRxiv - Neuroscience 2020Quote: Fresh-frozen human postmortem brain tissues were sectioned at 14-16 μm onto Superfrost Plus glass slides (Fisher Scientific). Sections were dried for 20 minutes at −20°C and then vacuum sealed and stored at −80°C until use ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Gene expression was performed on a BioRad CFX96 real time PCR machine using gene-specific FAM-labelled TaqMan probe assays: human GAPDH control ID: Hs99999905_m1(ThermoFisher), zebrafish abcb4 ID ...
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bioRxiv - Cancer Biology 2020Quote: ... wells were filled with 250μl of human liver organoid isolation medium consisting Ad+++ supplemented of 1X B27 supplement without retinoic acid (Gibco), 1X N2 supplement (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... and resuspended in complete medium in the absence of human serum or Pf-iRBCs schizonts were isolated in RPMI 1640 medium supplemented with 0.25% Albumax (GIBCO, Invitrogen) and 7.4% Sodium Bicarbonate (GIBCO ...
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bioRxiv - Bioengineering 2021Quote: ... Another subset of slides was labeled with Alexa 568 conjugated anti-human IgG antibody (1:800, ThermoFisher, A-21090) to detect the Fc portion of the EphA4-Fc chimera peptide.
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: Microarray analyses were conducted using the human Clariom D Affymetrix platform following the manufacturer’s instructions (902915, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... the coding sequence of the current human textitTRBP reference (RefSeq NM_134323) was amplified and subcloned into the pcDNA3-cMyc vector (Invitrogen) at the BamHI and XhoI sites ...
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bioRxiv - Cancer Biology 2020Quote: CAFs were isolated from human colon carcinoma resection specimens following 2.4U/ml dispase + 100U/ml collagenase type II (both from Invitrogen); disaggregation of tumour fragments and digestion for 2h t 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and hybridized to the Affymetrix Human Gene 1.0 ST Arrays using the Gene Chip WT Terminal Labeling and Hybridization Kit (Affymetrix). GeneChip fluidics station 450 was used for processing of the arrays and fluorescent signals were detected with the GeneChip scanner 3000-7 G ...
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bioRxiv - Bioengineering 2021Quote: ... media was changed to the following formulation of EC medium: human endothelial serum-free medium (hESFM, Thermo Fisher 1111044) supplemented with 1% platelet-poor plasma-derived serum (PDS ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Immunology 2020Quote: Human PBMCs were incubated with MitoSOX Red (5μM) and Mitotracker Green (1 μM) (both from Molecular Probes, Life Technologies) in PBS during 10 minutes at 37°C before flow cytometry extra-cellular staining.
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bioRxiv - Genomics 2021Quote: 40-80 ng of each probe (labeled with biotin or Dig) and 1µg of unlabeled human COT1-DNA (Invitrogen) were dissolved in 4µl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from native pig and human lung tissue (fresh snap-frozen) was isolated using the PureLink Mini Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Human ASGR1 and its mutants (Q240A/W244A and Q240A/W244A/E253A) and human ASGR2 were sub-cloned into pcDNA 3.1 vector (Invitrogen) containing a C-terminal Flag tag (ASGR1 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Developmental Biology 2021Quote: A human episomal iPSC line derived from CD34+ cord blood was used in this study (A18945, ThermoFisher Scientific; [58]). This cell line has already been validated in its ability to generate light-sensitive 3D retinal organoids ...