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Citations for Thermo Fisher :
5751 - 5800 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... in 1x SS-IV RT buffer containing 10 units of RNase OUT (Invitrogen), 5 mM DTT ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized using a Thermoscript RT III kit (Invitrogen, USA). Gene specific primers (GmSALT3_gF and GmSALT3_gR ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed in triplicate using SYBR Select Master Mix (ThermoFisher Scientific) using a StepOnePlus Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... or by RT-qPCR analysis of mRNAs purified using TRIzol reagent (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... First-strand cDNA was generated from total RNA using SuperScript RT IV (Invitrogen) and IgA and IgG isotype specific primers 30 including UMIs at 50 °C for 45 min (inactivation at 80 °C for 10 min).
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bioRxiv - Biochemistry 2021Quote: ... RT-qPCR samples were prepared in 96-well plates (4306737, Applied Biosystems, USA) by mixing 5 μL of 2 × iTaq universal SYBR green reaction mix ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription (RT) was performed in a Veriti 96-well thermal cycler (ThermoFisher). After RT ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl of 200 U/µL SuperScript III RT enzyme (Invitrogen, 18080044). Following reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl of 200 U/μL SuperScript III RT enzyme (Invitrogen, 18080044). The reaction product (in the form of a cDNA:RNA hybrid ...
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bioRxiv - Pathology 2021Quote: ... incubated (without detergent-based permeabilization) with AlexaFluor 594 Phalloidin (Invitrogen; 20 min, RT) to visualize actin cytoskeleton ...
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bioRxiv - Genomics 2020Quote: ... at RT in 1% freshly prepared Formaldehyde in PBS (ThermoFisher, Cat. N: 28908) under slow rotation and quenched by addition of Glycine (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Power-Up SYBR Green Master Mix (Thermo Fisher) using a Quant Studio Flex 6 (Applied Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... and RT-qPCR performed using the ViiA 7 system (Applied Biosystems/ThermoFisher Scientific) as previously described[54] ...
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bioRxiv - Immunology 2020Quote: ... and RT-qPCR performed using the ViiA 7 system (Applied Biosystems/ThermoFisher Scientific) as previously described[54] ...
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bioRxiv - Immunology 2021Quote: ... The RT-qPCR reaction used TaqPath 1-Step Multiplex Master Mix (Thermo Fisher) along with 2019-nCoV RUO Kit (IDTDNA ...
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bioRxiv - Cell Biology 2021Quote: ... were coated for 4 h at RT with rat-tail collagen-1 (Gibco) in 0.02 M acetic acid for a resultant 8.3 µg/cm2 collagen density ...
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bioRxiv - Genomics 2021Quote: ... 2.75 ul of RT mix was added using a Multidrop Combi (ThermoFisher, 5840300). Once the assay plates were thawed ...
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bioRxiv - Developmental Biology 2022Quote: ... RT-qPCR was performed using TAQMAN FAST ADVANCED MMIX (Applied Biosystems, Cat#4444557) using custom designed Taqman Probes for Nppa (Assay ID ...
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bioRxiv - Cell Biology 2022Quote: ... Vitronectin coated plates (Life Technologies, A14700, 1:100 in DPBS 1h at RT) were used to culture BIONi010-C iPSCs with StemFlex medium (Fisher scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... reagents used for the reverse transcription (RT) reaction such as Superscript II (Invitrogen) and RNasein Plus (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR was performed with Fast SYBRGreen Master Mix (Applied Biosystems, Cat#4385614) and run on Applied Biosystems StepOnePlus Real Time PCR system with StepOne v2.3 software ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... RT-qPCR was performed using PowerUP SYBR Green Master Mix (Applied Biosystems, A25742) in a QuantStudio 3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems).
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bioRxiv - Microbiology 2022Quote: ... RNA was reverse-transcribed using SuperScript® II RT (Invitrogen, Carlsbad, CA, USA), oligo dT and random primers ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using Fast SYBR Green Master Mix (Thermo Fisher, 4385616) on a QuantStudio 5 instrument (Applied biosystems).
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bioRxiv - Microbiology 2022Quote: ... Ligated RNA was then reverse transcribed to cDNA with SuperScript III RT (Invitrogen) using random primers of different lengths that also include a known sequence adapter (long and short RT primers ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR reactions were performed in a QuantStudio 5 System (Applied BioSystems, USA) under the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... Ligated RNA was reverse transcribed to cDNA using MMLV RT (Thermo Fisher Scientific) per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Maxima first strand cDNA synthesis kit for RT-qPCR with DNase (Thermo Scientific), and the SYBR Green Master Mix (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... Barcoded cDNA of each sample was generated with a Maxima RT polymerase (ThermoFisher) using oligo-dT primer containing barcodes ...
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bioRxiv - Immunology 2022Quote: ... a template-switch adaptor (5’-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG) and the Superscript II RT enzyme (Invitrogen). The cDNA was then purified using AMPure XP Beads (Agencourt) ...
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bioRxiv - Immunology 2022Quote: ... cells were permeabilized with permeabilization buffer for 20 min at RT (Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Physiology 2024Quote: ... Permeabilized tissue sections were then washed with 1X Maxima RT buffer (EP0751, Thermofisher). Reverse transcription was conducted on the array through the incubation of RT mixture (1X RT buffer (EP0751 ...
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bioRxiv - Physiology 2024Quote: ... and reagents for performing reverse transcription and RT-qPCR were from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR and qPCR were then performed on a QuantStudio 7 Flex (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription (RT) was conducted using SuperScript IV Reverse Transcriptase (Thermo Fisher, 18090050) with RT primer GGGCGGCAAGATCGCCGTG ...
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bioRxiv - Microbiology 2024Quote: ... followed by RT-qPCR analysis using PowerUp SYBR Green Master Mix (Applied Biosystems), utilizing ISG-specific primers that were described before 76 and are listed in the Table S2 ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were brought to RT (10 min) and disuccinimidyl dibutyricurea (DSBU, ThermoFisher, A35459) was added to a final concentration of 0.5 mM ...
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bioRxiv - Microbiology 2024Quote: ... were determined by RT-qPCR (SuperScript III First Strand Synthesis System, Life Technologies and PowerUP SYBR Green Master Mix ...
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bioRxiv - Cell Biology 2024Quote: ... blocked for 1h at RT in blocking solution (5% rabbit serum (10510, ThermoFisher) and 1% BSA (A4503 ...
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bioRxiv - Immunology 2024Quote: ... RT-qPCR was performed with absolute qPCR SYBR Green Mix (Thermo Fisher Scientific) using Roche LightcyclerTM 175 ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA were reverse transcribed using TaqMan RT reagents (Applied Biosystems) with random hexamers following manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... Reverse transcription (RT) was performed in a Veriti 96-well thermal cycler (ThermoFisher). After RT ...
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bioRxiv - Immunology 2022Quote: ... RT-reaction was completed using SuperScript™ III Reverse Transcriptase (18080-400, Invitrogen). qRT-PCR analysis was completed using a Quant-Studio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR data were then acquired on a QuantStudio 5 (Thermo Fisher Scientific) and normalized as [2^-(CtBst− - CtMRT− ...
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bioRxiv - Genetics 2023Quote: ... RT-qPCR reaction was performed with SYBR Green Master mix (Applied Biosystems; A25742) in the 7500 or QuantStudio 6 flex Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µl Invitrogen™ SuperScript® IV Reverse Transcriptase (RT) (Thermo Fisher Scientific), 1 µl 10 mM dNTPs (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed in a Fast SYBR Green Master Mix (Applied Biosystems) containing 12.5 ng cDNA template ...