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Citations for Thermo Fisher :
6001 - 6050 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... for 20 min at room temperature before fixation/permeabilization with the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen) and intracellular staining for 40 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was set up with cDNA as template and PowerUp™ SYBR™ Green Master Mix (Thermo Fisher, A25742) or TaqMan® Fast Advanced Master Mix (Applied Biosytems ...
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bioRxiv - Neuroscience 2023Quote: We used the QuantiGene multiplex system to simultaneously measure the expression of multiple genes in siRNA-treated SHSY5Y cell samples.52,53 Individual reagents and probe sets were purchased from ThermoFisher. Probes were directed against ...
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bioRxiv - Physiology 2022Quote: ... All MS and MS/MS raw spectra of TMT experiments from each set were processed and searched using Sequest HT algorithm within the Proteome Discoverer 2.2 (PD2.2 software, Thermo Scientific). The subsequent analyses were adapted from our previous work (36) ...
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bioRxiv - Molecular Biology 2024Quote: ... and two sets of triplicate technical replicates were run on NuPage 4-12% Bis-Tris SDS Page (ThermoFisher Scientific) gels in 10 mM DTT/1x loading buffer ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... cells were fixed and permeabilized with eBioscience FOXP3/Transcription factor staining buffer set (Thermofisher Scientific, Cat#00-5523-00).
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bioRxiv - Immunology 2024Quote: ... for 5h and harvested for intracellular cytokine staining with the FOXP3/Transcription Factor Staining Buffer Set (eBioscience, ThermoFisher Scientific), according to manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5% CO2 and then fixed and permeabilized using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen, 00-5523). Cells were stained with primary and secondary antibodies as described previously for immunofluorescence ...
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bioRxiv - Immunology 2023Quote: ... but then pelleted and resuspended in 100 μL fixative from the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen) for 30 mins at 4°C while protected from light ...
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bioRxiv - Cell Biology 2023Quote: Two sets of USP3 shRNAs were designed against its target sequence and cloned independently into pSilencer2.1-U6 hygro vector (Ambion). Scrambled shRNA with limited homology to any known sequences was cloned into the same vector and could serve as the negative control ...
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bioRxiv - Biochemistry 2023Quote: ... A total set of 104 tilt series was imaged on a Titan Krios G3i electron microscope (Thermo Fisher Scientific) operated at 300 kV ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized using FoxP3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific Cat# 00-5523-00) and stained with anti-mouse antibodies targeting intracellular markers according to standard protocols.
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bioRxiv - Cancer Biology 2022Quote: ... Fixation was performed with 4% paraformaldehyde or Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; 00-5523-00) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cell fixation and permeabilization was performed using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cell fixation and permeabilization was performed using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and set in a maintenance medium that contained 4% BSA Fraction V (Gibco) and 0.1% L-(tosylamindo-2-phenyl ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with PBS and permeabilized using the Foxp3 Transcription Factor Staining Buffer Set (Invitrogen, 00-5523-00) for 1 h in dark at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeablizied using eBioscience Intracellular Fix & Perm Buffer Set (Thermo Fisher, cat. no. 88-8824-00).
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bioRxiv - Immunology 2023Quote: ... Cells were then fixed and permeabilized with the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Invitrogen, Waltham, MA) to allow intracellular staining for Ki67 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then fixed and permeabilized using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00) following manufacturer’s recommended procedure ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells then underwent fixation and permeabilization using an Biosciences Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00) following manufacturer’s recommended procedure ...
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bioRxiv - Immunology 2023Quote: ... All MS and MS/MS raw spectra from each set were processed and searched using Sequest HT algorithm within the Proteome Discoverer 2.2 (PD2.2 software, Thermo Scientific).The settings for precursor mass tolerance was set at 12 ppm ...
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bioRxiv - Immunology 2023Quote: ... T cells were fixed and permeabilized using the eBioscience FoxP3/Transcription Factor Staining Buffer Set (Invitrogen, #00-5523-00) following manufacturer’s instructions and stained for intracellular cytokines for 30 minutes at room temperature ...
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bioRxiv - Zoology 2024Quote: ... Photographs of cross-sections set at the symphyseal midline were acquired using Avizo v.7.0 (Thermo Fisher Scientific, Waltham). First ...
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bioRxiv - Genetics 2024Quote: ... then with 5% being set aside as the Input and the rest being incubated with 5μl anti-GFP antibody (A-11122, Invitrogen) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed on the ABI 7900HT Real-Time PCR system (Applied Biosystems) using SYBR Green Master mix (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... PCR was conducted on the ProFlex 96-Well PCR System (Thermo Fisher Scientific). Thermal cycling conditions consisted of an initial step at 95 ◻ for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... All PCRs were run on a QuantStudio Real-time PCR machine (Applied Biosystems).
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative PCR analyses were performed using SYBR Green PCR Master Mix (Applied Biosystems) and primers listed in Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative real time PCR was performed on a StepOnePlus PCR machine (Applied Biosystems) using the FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were purified with GeneJET PCR Purification Kit (Thermo Fisher Scientific) and their purity was verified on 1% agarose gel with SYBR Safe reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed using Power SYBR® Green PCR master mix (ThermoFisher), primers specific for human Ifnlr1 (79 ...
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bioRxiv - Molecular Biology 2021Quote: ... Real time-PCR was performed using ViiA7 Real-Time PCR system (Applied Biosystems) with TaqMan gene expression assays (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR was performed using Phusion High-Fidelity PCR Kit (Thermo Fisher Scientific, US), and Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... A 10µl PCR reaction contained TaqMan Fast Universal PCR mastermix (ThermoFisher Scientific, 4366072), 0.1 μM Universal probe library hydrolysis probe ...
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bioRxiv - Microbiology 2019Quote: ... PCR assays were performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific). Strains and plasmids used in this study are listed in Table S1 ...
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bioRxiv - Biochemistry 2019Quote: ... This PCR fragment was first cloned into the pCR®-Blunt vector (Invitrogen), and then subcloned into the pCDNA3 vector ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR reactions were run with Fast Universal PCR master mix (#4352042, ThermoFisher) in a Bio-Rad CFX384 system (Koon et al. ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR products were purified with a GeneJET PCR Purification Kit (Thermo Fisher Scientific). These sequences were domesticated with FastDigest Esp3I (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems), on an Applied Biosystems StepOnePlus Real-time PCR system and the following conditions ...
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bioRxiv - Systems Biology 2020Quote: ... qRT-PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems inc.) on an ABI 7300 instrument (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR was performed in 7500 Real-Time PCR System (Applied Biosystems, USA) using 1/20 times dilution of the respective cDNAs ...
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bioRxiv - Developmental Biology 2021Quote: PCRs were carried out using 2X PCR Master mix (Taq Polymerase) by ThermoFisher Scientific (Catalogue No ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by q-PCR performed with SYBR Green PCR Master Mix (Life Technologies), Bullseye EvaGreen PCR Master Mix (Midwest Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR plates were run on StepOnePlus Real-Time PCR machines (Applied Biosystems) and data was initially analyzed using StepOne Software (Applied Biosystems) ...
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bioRxiv - Biochemistry 2021Quote: ... qRT-PCR were run and analyzed in Stepone Plus PCR system (Applied Biosystems).
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bioRxiv - Synthetic Biology 2021Quote: ... colony PCR was performed using DreamTaq Green PCR Master Mix (2X) (Thermo Scientific) with a colony resuspended in 50µl of water as described (REF openwetware protocol from Endy or Knight) ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was purified (Pure link Quick PCR purification kit, Invitrogen USA), cloned and both strands were sequenced (UMGC ...
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bioRxiv - Systems Biology 2020Quote: ... qRT-PCR was performed using the ViiA7 real-time PCR system (Applied Biosystems). Primers were generated using the Primer-BLAST design tool from NCBI based on the accession number of the gene of interest ...
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bioRxiv - Genetics 2020Quote: ... qRT-PCR was performed using a ViiA7 Real-Time PCR System (Applied Biosystems) (40 cycles at 95 °C for 15 sec and 60 °C for 1 min) ...