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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the eBioscience FOXP3/transcription staining buffer set (Invitrogen, Reference: 00-5523-00) and then stained for the intracellular proteins ...
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bioRxiv - Biochemistry 2022Quote: Cryo-EM data set of the FERRY complex was collected on a Titan Krios TEM (Thermo Fisher Scientific), equipped with an Cs-Corrector and in-column energy filter ...
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bioRxiv - Bioengineering 2022Quote: ... and intracellular staining according to the manufacturer’s protocols from eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Invitrogen). The following anti-mouse antibodies were used ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixated with 200 μL fixation buffer (eBioscience™ Foxp3/Transcription Factor Staining Buffer, Set, Invitrogen™) and incubated for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed (20min) and permeabilized (30min) on ice using the Foxp3 Transcription Factor buffer set (ThermoFisher Scientific) and incubated for an hour with TF antibodies ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... and intracellular staining was performed by using eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: Intracellular transcription factor stains were done using the eBioscience Foxp3/ Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) according to the supplier’s information ...
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bioRxiv - Cell Biology 2024Quote: ... This staining procedure was carried out with the Transcription Factor Staining Buffer Set (Invitrogen, Cat. # 00-5523-00).
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was performed using the eBioscience FoxP3/Transcription Factor Staining Buffer Set and protocol (Invitrogen, Waltham, MA). Stained cells were run on a CytoFLEX LX (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... Maximum release was set up with 2% Triton X-100 (Acros Organics, Thermo Fisher Scientific, Waltham, MA, USA). All samples and controls were set up in triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... A 5ng/μL cDNA reaction mix was set up according to manufacturer’s instructions (SYBR green MM, Fisher Scientific). Thermal cycling was performed at 50°C for 2 min then 95°C for 10 min then 40 cycles of 95°C for 15s ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were fixed for 1 h using the eBioscienceTM Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and stained according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The TMT-set was further purified using High Protein and Peptide Recovery Detergent Removal Spin Column (88305, ThermoFisher) and Pierce peptide desalting spin columns (89852 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and then blocked and permeabilized in eBioscience Foxp3/Transcription Factor Staining Buffer Set permeabilization buffer (Invitrogen, 00-5523) for another 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... An EiscoTM Superior Stand and Rod Set with a three-prong clamp with a Boss Head (Fisher Scientific) was used to stabilize the nebulizer ...
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bioRxiv - Microbiology 2023Quote: ... Intracellular staining for transcription factors was performed using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Invitrogen) with antibodies against granzyme B (QA16A02) ...
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bioRxiv - Microbiology 2023Quote: TMT labelling of the desalted phosphopeptides was carried out using TMTsixplex isobaric label reagent set (Thermo Fisher Scientific). The phosphopeptides of each experimental condition were dissolved in 100 µL of 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Immunology 2022Quote: ... splenic cells were fixed using Fixation/ Permeabilization buffer (Foxp3 / Transcription Factor Staining Buffer Set, #00-5523-00, Invitrogen) immediately after isolation at room temperature for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg peptide from each sample was labeled with TMT10plex Isobaric Label Reagent Set (ThermoFisher Scientific, cat# A37725) following to the manufacture’ s instruction ...
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bioRxiv - Immunology 2023Quote: ... and for Treg characterization cells were fixed with FoxP3/Transcription factor staining buffer set (00-5523-00, Invitrogen) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The cytospun cells were fixed and stained with a Protocol® HEMA3 stain set (Fisher Scientific, Kalamazoo, MI). Cellular/nuclear morphology was assessed by light microscopy ...
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bioRxiv - Immunology 2023Quote: ... Transcription factor staining was performed without pre-incubation using Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... MYC (Mr04269876_mr) and SeV (Mr04269880_mr) was subsequently set up using TaqMan™ Gene Expression Assay (FAM) (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... A 100% lysis rate was set using the A541nm from the group of 0.6% RIPA (Thermo Fisher Scientific) lysis buffer.
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bioRxiv - Developmental Biology 2024Quote: ... and extracted RNA was purified using Purelink RNA mini kit/Pure link DNase-set (Invitrogen/Thermo Fischer Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT-PCR was performed with a SYBR Green PCR Master Mix and the 7900 Real time PCR system (Applied Biosystems). Results were analyzed by the standard curve method and normalized to β-actin expression ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was used as template for quantitative PCR with PCR amplification detected with Sybr green (SYBR Green Real-time PCR master mixes, Invitrogen). See the table below for the sequences of primers used to quantify real time amplification.
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bioRxiv - Genomics 2019Quote: ... Quantitative real time PCR reactions were performed with the ABI 7900 Real Time PCR System using Universal PCR Master Mix (Thermofisher), with initial activation at 50°C for 120 seconds and 95°C for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using Power SYBR® Green PCR Master Mix (Thermo Fisher Scientific, Slovakia) and a QuantStudio 5 thermocycler (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The quantitative PCR (Q-PCR) reactions were conducted in triplicate on a ViiA 7 Real-Time PCR system (Thermo Fisher) from 1 μL of cDNA using the PerfeCTa SYBR Green SuperMix reagent (Quantabio Cat# ...
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bioRxiv - Microbiology 2022Quote: ... The resulting cDNA was diluted 1:50 in water and utilized for real-time quantitative reverse transcription PCR (qRT-PCR) in a QuantStudio 3 PCR system (Applied Biosystem) with SYBRGreen PowerUp reagent (Invitrogen), Primers used to amplify internal fragments of CPS biosynthesis genes are listed in Table S1 ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed in a Quantstudio 5 Real-Time PCR Instrument using SYBR Green PCR Master Mix (Applied Biosystems). Three biological replicates were carried out ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR (qRT-PCR) was performed using QuantStudio™ 12K Flex Real-Time PCR System (Applied Biosystems™). Primers used in this study are listed in (Table 2) ...
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bioRxiv - Developmental Biology 2019Quote: ... The TOPO XL PCR cloning kit was used to introduce the PCR fusion fragments into the pCR-XL-TOPO vector (Invitrogen). Mutations on LIN-39 motifs were introduced via mutagenesis PCR ...
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bioRxiv - Physiology 2019Quote: ... Quantitative Real-time PCR (qRT-PCR) was performed in duplicate using the ViiA 7 Real-time PCR system (Applied biosystems). All expression data were normalized by the 2(-ΔCt ...
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Mitochondrial ROS1 increases mitochondrial fission and respiration in oral squamous cancer carcinomabioRxiv - Cancer Biology 2020Quote: ... Q-PCR was performed using Power SYBR Green PCR Master Mix and ABI StepOnePlus Real-Time PCR System (Applied Biosystems) with specific primers ...
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bioRxiv - Neuroscience 2019Quote: ... These fragments were combined by fusion PCR and then subcloned into a pCR-Blunt vector with the Zero Blunt PCR Cloning Kit from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed using SYBR Select PCR master mix on a ViiA7 Real-Time PCR System (both from ThermoFisher). Fold changes were calculated by the 2−(ΔΔCt ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR was performed by StepOnePlus Real-Time PCR system using SYBR Green PCR master mix (Applied Biosystems). Primer sequences (Supplementary Table S1 ...
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bioRxiv - Plant Biology 2022Quote: ... Gene expression was accessed using real-time PCR (qRT-PCR) (Step One PlusTMReal Time PCR System, Applied Biosystems, CA, USA) with the SYBR green fluorescence detection (Applied Biosystems®) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was cloned in the pCR BluntII TOPO vector (Invitrogen). The resulting plasmid was digested first with EcoRV and after with XhoI/NdeI ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was cloned in the pCR BluntII TOPO vector (Invitrogen). The resulting plasmid was digested first with EcoRI and after with NdeI/SpeI and the fragment (877 bp ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed with a StepOnePlus Real-Time PCR System (ThermoFisher) using the Quantitect SYBR Green PCR kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified using EXOSAP-it PCR purification kit (Applied Biosystems) and sequenced with the ABI BigDye Terminator v ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quantitative PCR was done using Power SYBR PCR master mix (Thermo Fisher). SP1 occupancy of a given region after the immunoprecipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... qRT-PCR was performed on 7500 Real-Time PCR instrument (Applied Biosystems) using THUNDERBIRD® SYBR qPCR Mix (Toyobo life science ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR fragments were subsequently cloned into the pCR-bluntII-TOPO vector (Invitrogen). Plasmid DNA was isolated from single colonies and sent for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR was performed on a 7500 real-time PCR system (Applied Biosystems) using iTaq SYBR Green Supermix (BioRad) ...