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Citations for Thermo Fisher :
5801 - 5850 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and RT-qPCR were performed according to standard protocols (7300 AB; Applied Biosystems). The following gene of interests were quantified (Applied biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligated RNA was reverse transcribed using Maxima H Minus RT (Thermo Scientific, EP0752) at 60°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... Samples were then saturated for 24h at RT in a blockaid solution (Thermofisher) with 0,5% triton X-100 and 0,1% tween ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RT-qPCR was performed using the TaqMan Gene Expression Assays (Thermo Fisher Scientific), targeting human HPRT1 (Hs01003267_m1) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA synthesis was performed with random hexamers using the RevertAid RT Kit (Thermofisher). qRT-PCR was performed using the HOT FIREPOL EvaGreen qPCR mix plus (Solis Biodyne ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was further obtained using the High Capacity cDNA RT kit (Applied Biosystems). RNA-seq libraries were prepared using the Swift Biosciences RNA library kit according to manufacturer’s instructions and sequenced on an Illumina HiSeq2500 (SE50 ...
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bioRxiv - Molecular Biology 2023Quote: ... and amplified by RT-qPCR using PowerUp SYBR-Green MasterMix (Thermo Fisher Scientific), as described in Desideri et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time RT-qPCR was carried out on a StepOne System (Applied Biosystems) using the THUNDERBIRD Next SYBR qPCR Mix (Toyobo) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were brought to RT (10 min) and disuccinimidyl dibutyricurea (DSBU, ThermoFisher, A35459) was added to a final concentration of 0.5 mM ...
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bioRxiv - Physiology 2024Quote: ... and reagents for performing reverse transcription and RT-qPCR were from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 9μL RT mix (6.5μL smRNA Mix 2, 0.5μL RNAse inhibitor ((Invitrogen Cat. #AM2696), 2μL PrimeScript RT (200U/ul) ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using Power-Up SYBR Green Master Mix (Thermo Fisher) using a Quant Studio Flex6 (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... Permeabilized tissue sections were then washed with 1X Maxima RT buffer (EP0751, Thermofisher). Reverse transcription was conducted on the array through the incubation of RT mixture (1X RT buffer (EP0751 ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Immunology 2024Quote: ... and the MMLV-RT reverse transcriptase (Moloney Murine Leukemia Virus, Invitrogen Life Technologies). The relative quantification of the genes of interest was carried out in a 10 μl reaction volume in white ultraAmp 384 well PCR plates (Sorenson ...
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bioRxiv - Microbiology 2024Quote: ... and complementary DNA (cDNA) was synthesized using the SuperScript III RT kit (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was run by using Fast SYBRTM Green Master Mix (Applied Biosystems) and a Step One Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... then blocked for 1 hour at RT with 100% SuperBlock (Thermo Scientific; 37535). Primary antibodies for DNA damage assays were diluted in PBS with 10% SuperBlock at the following dilutions ...
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bioRxiv - Plant Biology 2024Quote: ... followed by first-strand cDNA synthesis using the RevertAid RT kit (Thermo Scientific) with oligo(dT)18 primer ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed with the High-Capacity RT Kit (Applied Biosystems 4368814). The resulting cDNA was then subjected to qPCR with the TaqMan Master Mix II with UNG (Thermo Fisher 4440038) ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were performed on an ABI Prism 7500 SDS (Applied Biosystems).
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was conducted using the PowerUP SYBR green master mix (A25778, ThermoFisher) in a QuantStudio 5 real-time PCR system (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated during 10 minutes at RT of DAPI (Invitrogen, Waltham ...
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bioRxiv - Microbiology 2020Quote: ... Fused PCR products were cloned into pCR-Blunt II-TOPO using the Zero Blunt TOPO PCR Cloning kit (Thermo Fisher Scientific), and subsequently subcloned into pGRG36 (35) ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the SYBR Green PCR Master Mix on a ViiA7 Real-time PCR system (ThermoFisher Scientific) according to manufacturer’s instructions with 3 biological replicates (embryos randomly selected from 3 clutches ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system (Applied Biosystems). Expression of genes was normalised to three housekeeping genes (Gapdh ...
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bioRxiv - Genomics 2020Quote: ... cDNA was diluted 20-fold in PCR-grade water and subjected to real-time quantitative PCR (qPCR) using SYBR Green PCR Master Mix (ThermoFisher 4309155) with gene specific ...
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bioRxiv - Genomics 2019Quote: We diluted mouse genomic DNA to 5 ng/ul and performed quantitative PCR using an Applied Biosystems 7500 Fast Real-Time PCR instrument and Power SYBR Green PCR Master Mix (Applied Biosystems) with the following cycling conditions ...
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bioRxiv - Physiology 2019Quote: ... Quantitative RT-PCR was carried out with either TaqMan™ Universal PCR Master Mix or SYBR Green PCR master mix on the QuantStudio 7 Flex Real time PCR system (Applied Biosystems). All reactions were carried out in either duplicate or triplicate and Ct values were obtained ...
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bioRxiv - Plant Biology 2020Quote: We performed the quantitative reverse transcription-PCR (qRT-PCR) assays using SybrGreen Takara Master Mix in a 7500 Fast Real-Time PCR system (Applied Biosystems). For PCR ...
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bioRxiv - Cell Biology 2021Quote: ... was used for quantitative real-time PCR using either StepOne Plus Real-Time PCR system or Quant Studio 12K Flex Real-Time PCR Sytem thermocycler (Applied Biosystems). For each reaction technical duplicates and at least three biological replicates per experiment were performed ...
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bioRxiv - Cell Biology 2022Quote: ... real-time PCR was performed using Powerup SYBR Green PCR master mix (A25743) and a Step-One Plus realtime PCR machine (Applied Biosystems). Human Actin expression was used for normalization.
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bioRxiv - Pathology 2022Quote: ... Ten nanograms of cDNA was added to a 20-μL PCR reaction using SYBR Green PCR Master Mix or TaqMan Fast Universal PCR Master Mix (Applied Biosystems) on StepOne Plus System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: Real-time PCR was performed using the Applied Biosystems 7900HT Fast Real-Time PCR System with SYBR green PCR Master Mix (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was cloned into the pCR Blunt II-TOPO vector using the Zero Blunt TOPO PCR cloning kit (Thermo Fisher). The LINE-1 promoter-luciferase reporter plasmid was constructed by subcloning the promoter fragment into the KpnI-XhoI restriction site of the pGL4.11[luc2P] vector (Promega ...
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bioRxiv - Immunology 2020Quote: ... Quantitative PCR was performed with QuantaFast SYBR Green PCR mix (Qiaqen) on a 7500 Fast Real-Time PCR system (Applied Biosystems). The following primers were used: mGAPDH F ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real time-PCR (QRT-PCR) was run on a 7300 real-time PCR system (Applied Biosystems – Life Technologies, Carlsbad, CA). mRNA levels were normalized to 3 housekeeping genes ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real time-PCR (QRT-PCR) was run on a 7300 real-time PCR system (Applied Biosystems – Life Technologies, Carlsbad, CA). mRNA levels were normalized to 3 housekeeping genes ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed in 384 well PCR plates in an ABI 9700 PCR system (Applied Biosystems Inc., Foster City, CA, USA), and consisted of initial denaturation for 10 min at 95°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative-Real-Time-PCR (qRT-PCR) was performed in a 7500 Fast Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific) using TaqMan® Universal PCR Master Mix ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time PCR was performed on the QuantStudio 7 Flex Real-Time PCR System using Power SYBR Green PCR Master Mix (Applied Biosystems). The PCR conditions were 950C for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... Quantitative real time PCR (qRT-PCR) reactions were performed in the StepOnePlus or Quant Studio6 Flex Real Time PCR System (ABI, Thermo Fisher) with ten-fold dilution of cDNA and 200 nM of each primer using the SYBR Select PCR Master Mix (ABI ...
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bioRxiv - Microbiology 2019Quote: ... For cloning of UGV-1 GPC and HISV GPC we used RT-PCR amplification (reverse transcription with RevertAid Reverse Transcriptase, ThermoFisher Scientific; PCR with Phusion Flash High-Fidelity PCR Master Mix, ThermoFisher Scientific) from RNA extracted (by GeneJET RNA Extraction Kit ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR System and Power SYBR Green PCR Master Mix (Applied Biosystems). Relative liver parasite levels were quantified using the ΔΔCt method comparing levels of P ...
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bioRxiv - Molecular Biology 2019Quote: ... the 3’ end of rpc37 was first amplified by PCR and cloned into pCR Blunt II-TOPO using the Zero Blunt II TOPO PCR Cloning Kit (Invitrogen Life Technologies). Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription–quantitative PCRs were performed and analyzed using SYBR green PCR Master Mix and a StepOnePlus Real-Time PCR system (Applied Biosystems). Primer sequences are ACTB (endogenous control ...
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bioRxiv - Biochemistry 2021Quote: ... quantitative real-time PCR was performed in 5 μl of SYBR Green PCR master mix using a real-time PCR system (Applied Biosystems). Amplification products were then analyzed by a melting curve ...
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bioRxiv - Cell Biology 2021Quote: ... we performed quantitative Real-time PCR (qRT-PCR) assays by using SYBR™ Green PCR Master Mix (Applied Biosystems, Waltham, MA). These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time PCR was performed using the StepOnePlus Real-Time PCR System and Power SYBR Green PCR Master Mix (Applied Biosystems). Relative liver parasite levels were quantified using the ΔΔCt method comparing levels of P ...
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bioRxiv - Plant Biology 2020Quote: ... quantitative real-time PCR (qRT-PCR) assays were performed in the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, USA) using TB Green Premix Ex Taq II (Takara ...