Labshake search
Citations for Thermo Fisher :
5851 - 5900 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Quantitative-Real-Time-PCR (qRT-PCR) was performed in a 7500 Fast Real-Time PCR system (Applied Biosystems, Thermo Fisher Scientific) using TaqMan® Universal PCR Master Mix ...
-
bioRxiv - Microbiology 2022Quote: ... and bla-SHV) was carried out by conventional polymerase chain reaction (PCR) with PCR master mix (DreamTaq Green PCR Master Mix; Thermo Scientific) using specific primers (Table 1).
-
Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... The quantitative Reverse Transcriptase-PCR (qRT-PCR) was performed on the QuantStudio™ 12K Flex Real-Time PCR System (Applied Biosystems) with PowerUp™ SYBR Green Master Mix (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was performed using the TaqMan Universal PCR Master Mix (Applied Biosytems) and a 7500 Real-Time PCR System (Applied Biosystems). Each condition was performed in duplicate ...
-
bioRxiv - Plant Biology 2023Quote: ... Quantitative reverse-transcription-PCR (qRT-PCR) was performed on a QuantStudio™ 6 Pro Real-Time PCR System (Applied Biosystems A43182) in 384-well plates ...
-
bioRxiv - Cancer Biology 2023Quote: ... qRT-PCR was performed using SYBR Green PCR with the StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA). The following primer sets were used ...
-
bioRxiv - Cancer Biology 2023Quote: ... 30 ng of cDNA was used to perform the qRT-PCR reaction using SYBR green PCR master mix on an ABI Quant Studio 6 Flex Real-Time PCR System (Applied Biosystems). The housekeeping genes GAPDH ...
-
bioRxiv - Immunology 2023Quote: ... Quantitative PCR (Q-PCR) analysis was performed using SYBR Green Real-time PCR Master Mix on a Real-Time PCR System (Applied Biosystems). The primer sequences utilized for the Q-PCR amplification are provided in the supplemental Table 2.
-
bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (4368708, ThermoFisher Scientific). Primers for HA (5’- AAACTCTTCGCGGTCTTTCCA-3’ sense sequence and 5’-GATAAGGTAGCTTGGGCTGC-3’ antisense sequence) ...
-
bioRxiv - Microbiology 2024Quote: ... The quantitative real-time PCR (qRT-PCR) mixture for each reaction sample consisted of 10 μL of 2× SYBR green PCR master mix (Applied Biosystems), 7 μL of nuclease-free water ...
-
bioRxiv - Biochemistry 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in a 96-well format using the StepOne Plus Real-Time PCR system (Applied Biosystems) and SYBR Select Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
-
bioRxiv - Microbiology 2020Quote: ... was performed per manufacture’s recommendations with 150 ng of random primers (Thermo Fisher Scientific) in 20 μl final volume ...
-
bioRxiv - Neuroscience 2020Quote: ... One microgram of RNA was used to synthesize cDNA using random primers (Life Technologies) and SuperScript III Reverse Transcriptase (Life Technologies ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... RNA was reverse transcribed into cDNA using Oligo(dT)18 primer (Thermo Fisher Scientific) and RevertAid H Minus (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primer concentrations were 10 μM for each oligonucleotide (Thermo Fisher Scientific, Waltham, MA, USA). Evaluation of results was performed in the CFX96 Touch RT-PCR Detection System program (Bio-Rad ...
-
bioRxiv - Neuroscience 2021Quote: ... and converted to cDNA using oligo-dT primers and Superscript II reverse transcriptase (Invitrogen). Quantitative RT– PCR was performed using Power SYBR Green PCR Master Mix (ABI ...
-
bioRxiv - Immunology 2021Quote: ... The isolated RNA was transcribed into cDNA using oligo(dT)18 primers (Thermo Scientific), random hexamer primers (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2019Quote: ... 3µg of mRNA was then reverse transcribed using Superscript II using oligodT primers (Invitrogen). Reverse transcriptase (RT)-PCR was performed at 42°C for 50 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... Fragmented RNA was reverse transcribed using random primers and SuperScript III (Thermo Fisher Scientific). After second strand synthesis ...
-
bioRxiv - Biochemistry 2019Quote: ... and the resulting RNA was reverse transcribed with Superscript III (Thermofisher, #18080093, primer below). The cDNA was amplified for 6-16 cycles (primers below ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... Primers and probe for BDNF exon IV and VI were purchased from Life technologies (BDNF exon IV ...
-
bioRxiv - Microbiology 2019Quote: ... 0.5 μM of each primer and 1 U of Taq DNA Polymerase (Invitrogen, EU). DNA amplification was performed in a GeneAmp® PCR System 9700 thermal cycler (Perkin-Elmer) ...
-
bioRxiv - Cancer Biology 2019Quote: ... and first-strand cDNA was synthesized using random hexamer primers and Superscript III (Invitrogen). Quantitative PCR (qPCR ...
-
bioRxiv - Plant Biology 2020Quote: ... using Gibson Assembly NEB Mix and overlapping primers following manufacturer’s recommendation (Thermo Fisher Scientific). The assembled plasmids were then recombined into pBGW destination vectors by LR recombination ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA and relevant primers were added to PowerUp SYBR Green Master Mix (Applied Biosystems) and real-time PCR performed using 7500 Fast Real-Time PCR System ...
-
bioRxiv - Cancer Biology 2019Quote: ... DNA and relevant primers were added to PowerUp SYBR Green Master Mix (Applied Biosystems) and real-time PCR performed using 7500 Fast Real-Time PCR System ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... Primers and probe for BDNF exon IV and VI were purchased from Life Technologies (BDNF exon IV ...
-
bioRxiv - Cancer Biology 2020Quote: ... cDNA and relevant primers were added to PowerUp SYBR Green Master Mix (Applied Biosystems) and real-time PCR performed using 7500 Fast Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2020Quote: ... after removal of primers and dNTPs with the ExoSAP-IT reagent (Affymetrix, CA, USA) as previously described.38 Additional targeted genotyping was performed as described in the appendix.
-
bioRxiv - Neuroscience 2020Quote: ... and cDNA was synthesized using Oligo(dT) primers and SuperScript III reverse transcriptase (Invitrogen). Target genes were amplified using SapphireAmp Fast PCR Master Mix (TaKaRa ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μl of Random Hexamer Primer (at 100 μM concentration, Invitrogen, Thermo Fisher Scientific), 1 μl of dNTP mix (10 mM concentration each) ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μl of Random Hexamer Primer (at 100 μM concentration, Invitrogen, Thermo Fisher Scientific), 1 μl of dNTP mix (10 mM concentration each) ...
-
bioRxiv - Plant Biology 2020Quote: ... random nonamer primers and the reverse transcriptase RevertAid H Minus (Thermo Fisher Scientific, Inc.) according to the manufacturer’s manual ...
-
bioRxiv - Microbiology 2020Quote: ... 500 ng were then reverse-transcribed with random primers and MMLV Reverse Transcriptase (Invitrogen). Quantification of mRNA was obtained by real-time PCR using the Kapa Sybr fast qPCR kit on a CFX96 Bio-Rad thermocycler ...
-
bioRxiv - Cancer Biology 2020Quote: ... cDNA and relevant primers were added to PowerUp SYBR green master mix (Applied Biosystems) and real-time PCR was performed using 7500 Fast real-time PCR system ...
-
bioRxiv - Plant Biology 2022Quote: ... Complementary DNA was synthesized from 500 ng of total RNA using random primers (Thermofisher) and SuperScript™ III Reverse Transcriptase (Thermofisher) ...
-
bioRxiv - Microbiology 2022Quote: ... Remaining primers and dNTPs were digested by adding exonuclease I (10 U, Thermo Fisher) and shrimp alkaline phosphatase (1 U ...
-
bioRxiv - Physiology 2020Quote: ... and primers listed in Supplementary Table 1 using a Step One Plus (Applied Biosystems) qPCR machine ...
-
bioRxiv - Neuroscience 2020Quote: ... and reverse transcribed using random hexamer primers and Maxima Reverse Transcriptase (Thermo Fisher, #K1642Invitrogen). cDNA was prepared with SYBR Green Master Mix (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... qRTPCR was conducted with TaqMan primers and reagents (Life Technologies Inc., Carlsbad, CA, USA). Data are presented as fold changes calculated from least squares mean differences according to the 2−ΔΔCt method 62 and normalized to B2M ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized with Superscript IV First Strand Synthesis System using dT primers (Invitrogen). qPCR was performed using SYBR-Green Mastermix (SA Biosciences ...
-
bioRxiv - Cell Biology 2022Quote: ... 400 nM of each primer and PowerUP SYBR Green Master Mix (Thermo Fisher Scientific). Three technical replicates of each biological sample were analysed using a Rotor-Gene Q 2plex (Qiagen ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA and primers were mixed with PowerUp SYBR green 2X master mix (ThermoFisher Scientific), followed by mRNA quantification with QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2022Quote: ... with Sarbeco E gene primers and probe in a QuantStudio 3 thermocycler (Applied Biosystems). Percent inhibition of virus infectivity was determined by comparing viral load in treated wells with the viral load in untreated control wells ...
-
bioRxiv - Biochemistry 2022Quote: ... 400 nM of each primer and PowerUP SYBR Green Master Mix (Thermo Fisher Scientific). Three technical replicates of each biological sample were analysed as previously [1] ...
-
bioRxiv - Molecular Biology 2019Quote: ... cDNA and relevant primers were added to PowerUp SYBR Green Master Mix (Applied Biosystems) and real-time PCR performed using 7500 Fast Real-Time PCR System ...
-
bioRxiv - Systems Biology 2019Quote: SuperScript II Reverse Transcriptase with oligo(dT) primer (all from Invitrogen, Carlsbad, CA, USA) was used to synthesize 20 μl cDNA templates from 1 μg purified RNA ...
-
bioRxiv - Developmental Biology 2019Quote: ... RNA was reverse transcribed into cDNA using random primers (Life Technologies, Carlsbad, CA, USA) and Superscript IV Reverse Transcriptase (Life Technologies ...
-
bioRxiv - Biochemistry 2019Quote: ... and 0.5 μg/μl Oligo(dT)12-18 Primers (Life Technologies, Carlsbad, CA, USA) per 20 μl reaction ...