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Citations for Thermo Fisher :
5701 - 5750 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 5.7μl of reverse transcription mixture (0.5μl Superscript II RT (200 U/μl; Invitrogen), 0.25μl RNase inhibitor (20 U/μl) ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR was performed in a StepOnePlus(tm) qPCR system (Thermo Fisher Scientific) using THUNDERBIRD SYBR qPCR Mix (Toyobo) ...
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bioRxiv - Neuroscience 2021Quote: ... for 15 min at RT and mounted with Prolong Gold Antifade Reagent (Invitrogen). Imaging was performed using a Nikon-A1 confocal microscope in the basilar pontine nuclei (denoted as pons) ...
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bioRxiv - Molecular Biology 2020Quote: ... 45 μL SuperScript™ III RT enzyme (200 U/μL, Invitrogen Cat #18080044), and 73 μL nuclease-free water (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... and RT-qPCR was performed using SYBR™ Select Master Mix (Thermo Scientific) on LightCycler®Instrument II (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... 4μL of RT mix were added in each well (2mM dNTP (Thermo scientific), 20mM DTT ...
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bioRxiv - Genomics 2019Quote: ... RT-qPCR samples were mixed with the following Taqman probes mixture (Applied Biosystems) and run on a LightCycler 96 (Roche) ...
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bioRxiv - Genomics 2020Quote: ... RT reactions were performed according to Superscript II protocol (Thermo Fisher Scientific #18064022) with random 9mer ...
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bioRxiv - Immunology 2019Quote: ... RT-qPCR was performed using Power-Up SYBR Green Master Mix (Thermo Fisher) using a Quant Studio Flex 6 (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... All RT-qPCR reactions were performed with 2x SYBR green master mix (ThermoFisher) on either an Applied Biosystems model 7500 real-time PCR machine or a QuantStudio 6 Flex ...
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bioRxiv - Immunology 2019Quote: ... Purified RNA (200ng) was reverse-transcribed to cDNA (M-MLV RT kit; ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using Power-Up SYBR Green Master Mix (Thermo Fisher) using a Quant Studio Flex 6 (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized using the High-Capacity cDNA RT kit from ThermoFisher (#4368814) from 1000 ng of total RNA ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR for VZV transcripts was performed with SYBR GreenER master mix (Invitrogen) using primers pairs indicated in Table S3.
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... The RNA was converted into cDNA using SuperScript IV RT (Invitrogen cat# 18090010) and Oligo(dT)20 Primer (Invitrogen cat# 18418020 ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed using TaqMan Fast Advanced Master Mix (ThermoFisher Scientific, USA) and the Applied Biosystems ViiA 7 Real-time PCR System (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: Real-time RT-qPCR was carried out using Taqman™ assays from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2020Quote: ... for 20 min at room temperature (RT) in Expi293™ expression media (Gibco). Cells were washed 3× in medium and once in PBS before fixation in 4% paraformaldehyde and 4% sucrose for 10 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using SYBR Green Master Mix (Applied Biosystems) with 5ng cDNA ...
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNA synthesis was done with a SuperScript II RT kit (Invitrogen) and random hexamers (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: RT was performed on 6μl of eluted RNA using MMLV reverse transcriptase (Invitrogen) with either a dT17 primer (for polyadenylated transcripts ...
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bioRxiv - Microbiology 2020Quote: ... stained with DAPI for 15min at RT and mounted using Fluoromont G (ThermoFisher) mounting medium.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was carried out using Superscript III RT enzyme (Invitrogen, Cat #18080044) following recommended manufacturer protocols ...
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bioRxiv - Immunology 2022Quote: ... stained for nuclei (DAPI – ThermoFisher Scientific 62248 – 1:2000 for 5 min RT) and mounted with Fluoromount G (ThermoFisher 00-4958-02) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.5 μg RNA converted to cDNA using Taqman RT reagents kit (Thermofisher) with random hexamer priming ...
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bioRxiv - Microbiology 2021Quote: ... and 1.0 μl SuperScript III RT/Platinum Taq High Fidelity Enzyme Mix (Invitrogen). Thermal cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was prepared using the High Capacity cDNA RT kit (Applied Biosystems #4374966) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The mRNA obtained was then retrotranscribed using the RT reverse transcription Kit (Thermofisher). Real time RT-PCR was performed using the qPCR Master Mix kit (Thermofisher ...
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bioRxiv - Microbiology 2021Quote: ... followed by RT-qPCR with PowerUp SYBR Master Mix (ThermoFisher cat no. A25742) on a QuantStudio 3 Real Time PCR System using gene-specific primers pairs for ACE2 and RPS11 as the reference gene ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were blocked for 1 hr at RT in BlockAid (Thermo Fisher Scientific) and incubated for 48 hr at 4°C with an antibody against RFP (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... for 15 min at RT and permeabilized with ice-cold methanol (Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... the RT reaction was performed in presence of ERCC RNA Spike-in (Ambion). Unused RT primers were digested using Exonuclease I (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... slides were treated with DAPI (1:500, 3 min RT, ThermoFisher Scientific, D1306), washed briefly in PBS (2x) ...
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bioRxiv - Developmental Biology 2019Quote: ... reverse transcription was performed using the RevertAid RT Reverse Transcription Kit (Life technologies), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The RT reaction was performed with oligo(dT)12-18 (Thermo Fisher Scientific) primer or target- and strand-specific primers using PrimeScript Reverse Transcriptase (Takara Bio ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl Superscript III RT/Platinum Taq HiFi Enzyme mix (Invitrogen, Stockholm, Sweden), 25 μl 2x reaction mix ...
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bioRxiv - Cancer Biology 2019Quote: ... and RT-112 (DSMZ) were grown in 90% RPMI 1640 (Life Technologies, CA) + 10% FBS with 100 U/ml penicillin-streptomycin in a 5% CO2 cell culture incubator.
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with DAPI (1µg/mL) for 10 min at RT (ThermoFisher). Acquisition was performed using an Apotome Zeiss at 10X (comet assays) ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription to cDNA was accomplished using SuperScript III RT enzyme (Life Technologies) and random hexamers ...
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bioRxiv - Genetics 2020Quote: ... The RT reaction was carried out using Superscript IV VILO Mastermix (Life Technologies) using 1ug of total RNA according to the manufacturer’s instructions and the resulting cDNA was stored at −80C ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA synthesis was carried out using SuperScript™ II RT (Thermo Fisher Scientific) and Oligo(dT)18 primers ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was generated using the High Capacity cDNA RT Kit (Applied Biosystems, 4368814) and stored at −20°C until further processing ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted RNA was converted to cDNA using the ABI-RT kit (Life technologies). Quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Synthetic Biology 2020Quote: ... for quantification by RT-qPCR with Fast SYBR Green Master Mix (Thermo Fisher). Barcode cassettes were quantified by oligos BC20v1-F and BC20v1-R ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using the High Capacity RT kit (Applied Biosystems, CA, USA). SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Pathology 2021Quote: ... Membranes were blocked for 1 hour at RT with 3% BSA (Thermo Fisher) in tris-buffered saline with 0,1% Tween (TBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... complementary DNA was synthesized using a high-capacity cDNA RT kit (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... and RT-qPCR was performed using Luminaris HiGreen qPCR Master Mix (Thermo scientific) in a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCRs were carried out in a StepOne Real-Time Cycler (Applied Biosystems). All primers (listed in Supplementary Table 1 ...