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Citations for Thermo Fisher :
5951 - 6000 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were diluted in a mix containing RP1 primer (Table 1) and dNTPs (Thermo Fisher), followed by denaturation at 65 °C for 1 minute and incubation in ice water for 1 minute ...
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bioRxiv - Cell Biology 2023Quote: ... Pre-amplification was performed using Taq-Man primers and Taq/SSIII reaction mix (Invitrogen), according to prep program ...
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bioRxiv - Molecular Biology 2023Quote: ... Primers were designed with a 30bp overlap with the pDest17 expression vector (Invitrogen: 11803012) for subsequent incorporation into this vector via Gibson assembly.
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription was performed after incubation of mRNA with Random Primers (ThermoFisher Scientific, 48190011) for 5 minutes at 70°C ...
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bioRxiv - Plant Biology 2024Quote: ... cDNAs and qPCR primers were combined with 2X SYBR master mix from Thermo Fisher for qPCR ...
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bioRxiv - Physiology 2024Quote: ... (2023) using Primer Express software version 3.0 (Applied Biosystems, ThermoFisher Scientific, Wilmington, DE, USA).
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bioRxiv - Microbiology 2024Quote: ... 2 µM PAGE-purified primers and one unit of Platinum Taq DNA polymerase (Invitrogen). qPCR signal from a mock control lacking antibody was measured to determine the efficiency of recruitment ...
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bioRxiv - Neuroscience 2024Quote: ... oligo(dT)20 primers and the SuperScript III First-Strand kit (Thermo Fisher Invitrogen). Amplification was performed using a LightCycler 480 (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... oligo(dT)20 primers and the SuperScript III First-Strand kit (Thermo Fisher Invitrogen). Amplification was performed using a LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2024Quote: ... cDNA synthesis was performed by random primers after DNase I treatment (TURBO DNase, Ambion) using the SuperScript III First Stand Synthesis system (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using random hexamer primers and RevertAid Reverse Transcriptase (Thermo Fisher Scientific). Real-time PCR experiments were run on a Mastercycler (Biorad) ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary DNA (cDNA) was synthetized using MultiScribe™ reverse transcriptase and random primers (Invitrogen) from 1.5 or 2 µg of RNA and diluted to 10ng/µl ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was reverse transcribed using random primers from a reverse transcription kit (Applied Biosystems). Quantitative PCR was performed using SYBR green master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... The following TaqMan primer probes were used: Snx12-FAM-MGB (Thermo Fisher Scientific, #Hs04999580_s1), Stx12-FAM-MGB (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... All TaqMan assays were designed using the Software Primer Express v3.0 (Applied Biosystems Inc.). TaqMan genotyping assays were carried out according to the standard protocol on an ABI 7500 Real-Time PCR System machine using Genotyping Master Mix (Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... cDNA was amplified using gene-specific primers and PowerUp SYBR Green Master Mix (Invitrogen) on a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was then generated using SuperScript III reverse transcriptase and oligo-dT primers (Invitrogen). Primers used to amplify Syrian hamster PKR from BHK-21 cells for RT-PCR analysis were BA16 and BA26 ...
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bioRxiv - Cell Biology 2022Quote: All MS and MS/MS raw spectra from each set were processed and searched using Mascot algorithm within the Proteome Discoverer 1.4 (PD 1.4 software, Thermo Scientific). Precursor mass tolerance was set at 20 ppm and fragment ion mass tolerance was set at 0.05 Da ...
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bioRxiv - Molecular Biology 2021Quote: ... sets of 10 worms (sex-separated) or five adult pairs were transferred to 0.4 cm pathway electroporation cuvettes (Invitrogen) containing DMEM supplemented with 2 mM L-glutamine and 100 µg/ml penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... ICFC was performed with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Catalog # 00-5523-00). Cells were surface stained as described above ...
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bioRxiv - Neuroscience 2019Quote: ... mRNA encoding for hyperPiggyBAC (VF1-20268-01) was detected using a custom probe-set designed by Affymetrix (now Thermofisher) using the Affymetrix ViewRNA ISH Tissue 1-Plex kit (Thermofisher ...
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bioRxiv - Biochemistry 2021Quote: ... TMT-labelling and fractionation according to previously described (24).TMT 10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific) was used despite two biological replicates of four conditions (a total of eight samples) ...
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bioRxiv - Immunology 2021Quote: ... samples were washed once more and resuspended in SET buffer before adding the styryl dye FM4-64 (#T13320, ThermoFisher) to a final concentration of 0.2 µg/ml [33] ...
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bioRxiv - Biochemistry 2021Quote: The combined SILAC-labeled protein samples (heavy/light) were labeled using iodoTMT sixplex Isobaric Label Reagent Set (Thermo Scientific) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2021Quote: ... two data sets were collected on the SFX incubated sample using a Titan Krios Cryo-TEM (Thermo Fisher Scientific) operating at 300 keV ...
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bioRxiv - Microbiology 2021Quote: TMT labelling of the desalted tryptic peptides was carried out using TMTsixplex isobaric label reagent set (Thermo Fisher Scientific). The peptides of each experimental condition were dissolved in 100 µL of 100 mM TEAB and treated with an RT equilibrated and freshly dissolved unique TMT label reagent in 41 µL anhydrous acetonitrile ...
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bioRxiv - Microbiology 2020Quote: ... RNA strands of YFV were detected following the manufacturer’s protocol (ViewRNA ISH Cells Assays) using probe sets designed by Affymetrix. The Alexa-Fluor 546-conjugated (+)-strand probe set targets a region between position nucleotides 4567 and 5539 of the YFV genome ...
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bioRxiv - Microbiology 2022Quote: ... and fixed for 30 minutes with fixation/permeabilisation buffer of eBioscience FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific). The cells were then washed with permeabilisation buffer ...
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bioRxiv - Physiology 2022Quote: ... Transfer was set at 35 V for 1h30 in a Xcell II Blot module (catalog no. EI9051, ThermoFisher Scientific). After transfer ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher) and stained for the following intracellular markers ...
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bioRxiv - Cancer Biology 2022Quote: ... we took advantage of a gene expression previously described data set of publicly available microarray data (Affymetrix HG-U133Plus2.0) consisting of 928 normal human tissue samples and 50 EwS samples32 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were permeabilized using eBioscience™ Foxp3/Transcription factor Staining Buffer Set (00-5523-00,Thermo Fisher Scientific) according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2020Quote: ... intracellular detection of IFITM3 was done using the eBioscience Foxp3 / Transcription Factor Staining Buffer Set (ThermoFisher; #00-5523-00) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Rabbit mAb (SinoBiological) and IFITM3 was subsequently performed using eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) followed by surface antigen staining.
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permabilized using the FoxP3/Transcription factor staining buffer set according to the manufacturer’s directions (ThermoFisher). APC-conjugated MHC class II (I-Ab ...
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bioRxiv - Neuroscience 2021Quote: ... . qPCR reactions were set up in duplicate reactions using the PowerUp SYBR Green Master Mix kit (Applied Biosystems #A25777) using an Mx3000P qPCR System (Agilent ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Treatment reactions (which were run in triplicate) were set up with 1ml of appropriate filtrate and 1ml of polysaccharide solution (PS) (0.9% polygalacturonic acid (ThermoFisher) in 0.1M sodium acetate (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the pelleted bacteria re-suspended in 2ml of SET buffer (65% v/v molecular grade H2 O (ThermoFisher); 20% v/v Tris (pH8) ...
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bioRxiv - Biophysics 2021Quote: High resolution data sets for both fragment complexes were collected on the Glacios using EPU software version 2.9.0 (ThermoFisher Scientific). Electron movies were collected in the counting mode at a nominal magnification of 150,000x yielding a pixel size of 0.97 Å ...
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bioRxiv - Neuroscience 2022Quote: ... To avoid genomic DNA contamination a DNAse step was performed using Invitrogen™ PureLink™ DNase Set (#12185010, Invitrogen). RNA quantity and purity were assessed with the Pico Kit Assay on the Agilent 2100 Bioanalyzer System.
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bioRxiv - Cell Biology 2022Quote: ... Four sets of three retinas per genotype were incubated in DMEM without methionine or cysteine (ThermoFisher Scientific. cat# 21013) but supplemented with 200 mM cysteine (SigmaAldrich C7352 ...
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bioRxiv - Microbiology 2020Quote: ... Gene expression for 22,238 probe sets of 12,490 genes was generated with U133A2.0 microarrays following the manufacturer’s instructions (Affymetrix, Santa Clara, CA). Drugs were profiled in duplicate or triplicate ...
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bioRxiv - Immunology 2020Quote: ... cells were permeabilized overnight at 4°C with the Foxp3/ Transcription Factor Staining Buffer Set according to the manufacturer’s instructions (ThermoFisher).Cell fluorescence was measured using an LSRII flow cytometer ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed and permeabilized using eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen, Catalog Number 00-5523-00) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... each sample was diluted to a set concentration of 100 ng µl−1 using the Qubit Fluorometer (Q32857, Invitrogen). For each biological sample and gene ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the eBioscience™ FOXP3 / Transcription Factor Staining Buffer Set (Invitrogen, 00-5523-00) and stained with anti-FOXP3 antibody according to the manufacturer’s protocol.
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Immunology 2020Quote: ... For intracellular flow cytometry cells were intracellular stained using the eBioscience™ Intracellular fixation & permeabilization buffer Set (Thermo Fisher) protocol using the PE conjugated CXCR2 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for transcription factors was performed using the eBioscience FoxP3/Transcription Factor Staining Buffer set according to manufacturer’s instruction (ThermoFisher). Cells were acquired on a BD LSRII or BD FACSAria III ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We set aside 10% of each sample as input and incubated the remaining chromatin with Dynabeads Protein G (Invitrogen) for 2-6 hours ...