Labshake search
Citations for Thermo Fisher :
5501 - 5550 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... with the primers and probes included in the TaqMan microRNA Assay kits (ThermoFisher, hsa-miR-210-3p ...
-
bioRxiv - Immunology 2024Quote: ... The total cDNA was obtained by reverse transcription using random primers from Invitrogen (ref ...
-
bioRxiv - Immunology 2019Quote: ... Quantitative real-time PCR (qRT-PCR) was performed in triplicate using Taqman® Universal PCR Master Mix (Applied Biosystems) in total reaction volumes of 20 μL and thermocycled in a CFX284 TouchTM Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Plant Biology 2021Quote: ... for quantitative real-time PCR (qRT-PCR) by using the QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems). The relative expression was analyzed by using QuantStudio 12K Flex software (Applied Biosystems) ...
-
bioRxiv - Cancer Biology 2019Quote: Quantitative reverse transcription PCR (qRT-PCR) was performed in triplicate using Power SYBR Green PCR Master Mix (Life Technologies). Samples were loaded into a BioRad CFX96 real-time PCR detection system following the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using Power SYBR green PCR Master Mix (Applied Biosystems) and the StepOnePlus Real Time PCR System (Applied Biosystems) ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on a QuantStudio 12K Flex Real-Time PCR System (Life Technologies) with a SYBR green detection protocol ...
-
bioRxiv - Pathology 2022Quote: ... quantitative realtime PCR (qRT-PCR) was performed using a Power SYBR Green PCR Master Mix Kit (Applied BioSystems, Inc). The primer sequences for the genes used in this study are listed in Supplementary Table 1.
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative reverse transcription-PCR (qRT-PCR) was performed on a Quant Studio 3 Real-Time PCR system (Thermo Fisher) with the KAPA SYBR Fast qPCR kit (KAPA Biosystems) ...
-
bioRxiv - Microbiology 2024Quote: ... The final PCR product was ligated with linear pCR-Blunt using a Zero Blunt PCR cloning kit (Life technologies) in DH5ɑ E ...
-
bioRxiv - Molecular Biology 2023Quote: ... The quantitative reverse transcription PCR (qRT-PCR) was run on the StepOnePlus Real-time PCR System (Applied Biosystems, USA), and relative quantification was performed using the 2−ΔΔCT method ...
-
bioRxiv - Cell Biology 2023Quote: ... PCR products were cloned into pCR-BluntII-Topo vector using Zero Blunt Topo PCR Cloning Kit (Thermo Fisher, 450245), sequenced ...
-
bioRxiv - Microbiology 2020Quote: ... Three independent sets of RNA samples for each strain were prepared using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2019Quote: ... the full-length GAD2 was also amplified using primer set GAD2_forward2 (5’-CACCATGGTTTTGACAAAAACCGC-3’) and GAD2_reverse and cloned into pENTR/D-TOPO vector via directional cloning (Invitrogen), followed by a LR reaction recombinant into pMDC3240 ...
-
bioRxiv - Cell Biology 2020Quote: ... Desalted peptides were labelled with TMTsixplex™ Isobaric Label Reagent Set (Catalog No. 90061, ThermoFisher Scientific, Waltham, MA) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were fixed using eBioscience™ FOXP3/Transcription Factor Staining Buffer Set (Thermo Fisher, cat. 00-5523-00) according to the protocol provided ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10% of beads were set aside for quality control and 90% resuspended in 1ml Trizol (Thermo Fisher Scientific) and stored at -80°C until further processing.
-
bioRxiv - Plant Biology 2019Quote: ... The digested peptides were labeled with TMT10plex™ Isobaric Label Reagent Set (90309, Thermo Fisher Scientific, lot TE264412) and combined ...
-
bioRxiv - Immunology 2019Quote: ... labeled with the E06 or PPARγ antibody intracellularly using the Intracellular Fixation & Permeabilization Buffer Set (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2019Quote: ... cells were fixed and permeabilized using ebioscience™ Foxp3/Transcription Factor Staining Buffer Set (ebioscience, Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... the container was quickly transferred to a vacuum oven set to 240 °C (Lindberg/Blue M, Thermo Scientific) and degassed at 25 inHg for 5 min ...
-
bioRxiv - Biophysics 2021Quote: ... a large data set for KATP channel structure determination was collected in Titan Krios microscope (Thermo Fisher Scientific) operated at 300 kV.
-
bioRxiv - Immunology 2021Quote: ... Intracellular staining for Ki67 was performed using the Transcription Factor Staining Buffer Set (ThermoFisher, cat. # 00-5523-00) following the manufacturers instructions ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR reactions of 10 µL were set up with 5 µL PowerUpTM SYBRTM Green Master Mix (Applied Biosystems), 500 nM of each primer OT5/OT and OT7/OT8 and 20 ng of DNA ...
-
bioRxiv - Cell Biology 2020Quote: The MS workflow parameters were set as follows using the Method Editor in XCalibur v3.0.63 (Thermo Fisher Scientific) for the SPS-MS3 acquisition method ...
-
bioRxiv - Biophysics 2021Quote: ... 2 ml of mix was allowed to set in the Lab-Tek chamber (Nunc™, Thermo Fisher Scientific) for 40 minutes ...
-
bioRxiv - Biophysics 2021Quote: ... 2 ml of mix was allowed to set in the Lab-Tek chamber (Nunc™, Thermo Fisher Scientific) for 40 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... Reactions were set up in 1X PowerUP SYBR Green master mix (Cat No. A25743, Thermo Scientific, PA, USA). ROX dye served as passive reference ...
-
bioRxiv - Microbiology 2021Quote: ... A comprehensive set of tissues were collected in either 10% neutral-buffered formalin (Fisher Scientific, Waltham, MA, USA), or as fresh tissues directly stored at −80°C ...
-
bioRxiv - Microbiology 2021Quote: ... A comprehensive set of tissues were collected in either 10% neutral-buffered formalin (Fisher Scientific, Waltham, MA, USA), or as fresh tissues directly stored at −80°C ...
-
bioRxiv - Biophysics 2022Quote: Both chaperones SET/TAF-Iβ-Q69C and NPM were fluorescently labeled using Alexa Fluor 532 C5 maleimide (ThermoFisher). NPM was labeled by means of its endogenous exposed Cys104 ...
-
bioRxiv - Plant Biology 2022Quote: ... All the probes of one set were pooled and en masse coupled with Alexa Fluor 647 (Thermo Fisher) or Texas Red ...
-
bioRxiv - Microbiology 2020Quote: ... A proprietary but publicly available probe set specific for positive sense ZIKV (Asian lineage) RNA was used (Thermofisher). Positive staining (red ...
-
bioRxiv - Cell Biology 2019Quote: ... SB-EM data sets were acquired with a FEG-SEM Quanta 250 (Thermo Fisher Scientific, FEI, Hillsboro, OR), using a backscattered electron detector (Gatan Inc. ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were permeabilized for intracellular staining using the Foxp3/Transcription factor staining buffer set (ThermoFisher, 00-5523-00) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: TMT samples were prepared according to the manufacturer’s instructions (TMTsixplex™ Isobaric Label Reagent Set, Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then fixed (eBioscience™ Intracellular fixation and permeabilization buffer set, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to a new set of tubes and lysed in 4X loading dye (Invitrogen, #NP0007) and boiled at 95℃ for 10 minutes ...
-
bioRxiv - Immunology 2021Quote: ... Surface staining was followed by cell permeabilization using FoxP3/transcription factor staining buffer set (Thermo Fisher Scientific, MA) at 4°C for at least 30 minutes ...
-
bioRxiv - Genomics 2022Quote: qPCR reactions were set up utilizing the 2 x PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, A25777) containing final 250 nM forward /reverse primer (s ...
-
bioRxiv - Immunology 2019Quote: ... cells were fixed and permeabilized using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... and then cDNA were quantified in multiplex TaqMan qPCR reactions using commercially available probe sets (Thermo Fisher 4453320) and TaqMan FastAdvanced Master mix (Thermo Fisher 4444556) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Peptides were labelled with 0.8 mg of TMT label (TMT10plex™ Isobaric Label Reagent Set, Thermo Fisher Scientific). Labelled samples were pooled ...
-
bioRxiv - Neuroscience 2019Quote: TMT labeling was performed according to the manufacturer’s instructions using the TMT10plex Isobaric Label Reagent Set (Thermo Scientific). In brief ...
-
bioRxiv - Cancer Biology 2020Quote: 3D acini were set up as described above in either 8-well coverglass chamber slides (Nunc, LabTek-II) or in 96 well plates (Greiner ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were fix and permeabilized using eBioscience FoxP3 / Transcription Factor Staining Buffer Set (ThermoFisher Scientific, 00-5523-00) and stained with 0.25μg/ml of mouse monoclonal yellow fever antibody (3576 ...
-
bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using buffer sets of Human Regulatory T-cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... and a fixation– permeabilization step was performed using the FOXP3 Transcription Factor Staining buffer set (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... Positive proliferation control wells were set up by plating 1 Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) for every 200 T-cells ...
-
bioRxiv - Immunology 2020Quote: ... serum samples or supernatants were analyzed by the Mouse IL-27 ELISA Read-Set-Go® Kit (Invitrogen) following the manufacturer’s’ instructions ...