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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was performed on Step One Plus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using TaqMan gene expression assays (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in Hibernate A buffer (NC1787837, Fisher Scientific) and incubated for 25-30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq assays were performed using the following antibodies: FOXA1 (Thermo Fisher Scientific: PA5-27157); AR (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... (Thermo Scientific) using electrospray ionization (ESI) ...
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bioRxiv - Cancer Biology 2024Quote: For total protein isolation, cells were lysed in RIPA buffer (Pierce, USA) containing protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific, USA). Cytoplasmic and nuclear portion of protein were extracted by NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... CM was prepared after drug or DMSO treatment by incubating cells in growth media containing 5% exosome-depleted FBS (Thermo Fisher Scientific, USA) for 72 h ...
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bioRxiv - Cancer Biology 2024Quote: ... FOXA1 (Thermo Fisher Scientific: PA5-27157); Vinculin (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... with DAPI (Prolong gold anti-fade, catalog no. P36931, Invitrogen/Fischer-Scientific). The multiplex staining was evaluated under confocal LMS900 system by Carl Zeiss.
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bioRxiv - Cancer Biology 2024Quote: ... Cytoplasmic and nuclear portion of protein were extracted by NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific, USA). Protein concentration was measured using the Bradford assay (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... Grids were imaged using transmission electron microscope Tecnai T12 (Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was mixed with SYBR Select Master Mix for CFX (Applied Biosystems, USA) and relevant primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) reaction was carried out with Step-One Plus system (Thermo Fisher Scientfic, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were cultured in DMEM medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... relative to the total cell number was counted in two to four different optic fields using EVOS microscope 40X objective lens (Thermo Fisher Scientific, USA). At least 200 cells were counted per sample.
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bioRxiv - Cancer Biology 2024Quote: ... FOXA1 (Thermo Fisher Scientific: PA5-27157); BRD4 (Diagenode ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transfected into HEK293-T or LNCaP cells using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... sEV isolation was performed using the Total Exosome Isolation Reagent (from cell culture media) kit (Invitrogen, USA) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA quality and concentration were determined using NanoDrop 2000 system (Thermo Fisher Scientific, USA). Reverse transcription of RNA to cDNA was performed using the iScript cDNA synthesis kit (Bio-rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... were transfected into cells using Lipofectamine 2000 reagent (Invitrogen, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: GeltrexTM LDEV-free reduced growth factor basement membrane matrix (Thermo Fisher Scientific A1413201) was defrosted on ice and diluted to 1 mg/mL in ice cold 0.01M Tris (pH 8.0) ...
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bioRxiv - Cancer Biology 2024Quote: ... MLECs were grown in DMEM:F12 (1:1) medium (Gibco), supplemented with 20% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... MM cell lines were maintained in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS (R&D Systems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic peptides extracted from each IP product were separated by reverse-phased nano-flow liquid-chromatography (EASY-nLC II, Thermo Scientific, San Jose, CA) and analyzed on an LTQ/Orbitrap Velos Elite hybrid mass spectrometer (Thermo-Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... the following antibodies were used: p300 (Invitrogen: 33-7600); CBP (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent dyes Hoechst (Invitrogen™, H1399) and Sytox™ Green (Invitrogen™ ...
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bioRxiv - Cancer Biology 2024Quote: ... CBP (Invitrogen: PA5-27369); AR (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 unit/mL of Penicillin and 100 mg/mL of streptomycin (Thermo Scientific, 15140122) with IL-7 (10 ng/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... before coated with red carboxylate-modified microspheres with diameters of 0.1 um (Thermo Fisher). Gel precursor solution containing 7.5% acrylamide and 0.15% bis-acrylamide was degassed for 30 minutes and mixed with 0.1% tetramethylethylenediamine and 0.1% ammonium persulfate before sandwiched between silane-activated coverslips and bead-coated coverslips for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... the cell pellet was resuspended in 1ml of stem cell media consisting of DMEM/F12 (Gibco, USA), 1% v/v Anti-anti (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... GBM39HSR and HK296 cell lines were cultured in DMEM/F12 (Gibco, 11320-033) supplemented with 1 × B27 (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using TRIzol (Invitrogen, USA). HiScript Q RT SuperMix (Vazyme,China ...
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bioRxiv - Cancer Biology 2024Quote: ... after which cells were stained for 1 hour at room temperature with AlexaFluor-conjugated secondary antibodies diluted in PBS+10% FCS (Life technologies) and stained with DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA (Gibco, 25300-054). Cells were then suspended in sterile phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng/mL hSCF (Life Technologies), 10nM EDN-1 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 10-20 % FBS (ThermoFisher, A5256701), 1% penicillin-streptomycin (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: 2×106 cells were seeded in 6 well plates and were incubated 24 hours later with 1uM of CellTrace Violet reagent (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin-streptomycin (ThermoFisher, 15140122) and passaged 1:4 every 3-4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Both CT2A and U87MG lines were maintained in culture using complete DMEM (Gibco, 11995-065, 10% FBS) and passaged using 0.05% Trypsin ...
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bioRxiv - Cancer Biology 2024Quote: ... Excess medium was removed and 200 μl of pre-warmed Neuron isolation enzyme (Gibco) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... counting beads (Invitrogen, C36950) were added to a fixed volume (i.e. ...
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bioRxiv - Cancer Biology 2024Quote: ... Small cell lung cancer cell line DMS53 was grown in Waymouth’s MB medium (Gibco, Invitrogen), supplemented with 10% FCS and gentamycin ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA (Gibco, 25300-054).
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 Pen-Strep (Gibco, 15140122), 1:1000 2-Mercaptoethanol (ThermoScientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with PrestoBlue reagent (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Melanoma IGR cell lines (IGR37 and IGR39) were grown in RPMI w/o HEPES (Gibco, Invitrogen) supplemented with 15% FCS and gentamycin ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was determined using Trypan Blue staining and counted using an automated cell counter (Applied Biosystems Countess 3). Cells were centrifuged at 300 x g for 5 minutes and resuspended in the appropriate volume of resuspension buffer (HBSS + 0.04% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 L-Glutamine (Gibco, 25030081), 1:100 Pen-Strep (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 Sodium Pyruvate (Gibco, 11360070), 1:100 L-Glutamine (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... in HBSS with calcium and magnesium (Gibco, 14175095). Tissue in digestion buffer placed in a dounce tissue homogenizer (Wheaton ...
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bioRxiv - Cancer Biology 2024Quote: ... The absorbance per well was measured with a CellInsight CX5 microplate reader (ThermoFisher). Determination of IC50 values was performed by nonlinear curve fitting using the Prism9 statistical software (GraphPad) ...