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Citations for Thermo Fisher :
5551 - 5600 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: The digested sticky ends were filled up with 10mM dNTPs (without dATP) and 0.4mM biotin-14-dATP (Life Technologies) and 40U DNA Pol I ...
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bioRxiv - Neuroscience 2020Quote: ... then conjugated with an alkyne-biotin compound according to the Click-iT Protein Analysis Detection Kit (Molecular Probes, C33372) protocol ...
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bioRxiv - Biochemistry 2021Quote: ... slides were incubated with goat anti-rabbit IgG biotin-SP conjugate secondary antibody (31752, 1:200, Invitrogen, Carlsbad, CA) and rabbit anti-rat IgG biotin-SP (31834 ...
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bioRxiv - Genomics 2022Quote: ... PCR products were labeled with biotin-16-dUTP or digoxigenin-11-dUTP using BioPrime CGH array labelling kit (Invitrogen), and then purified using BioPrime Purification Module (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... During this time the primary antibody was biotinylated according to manufacturer protocol (Thermo Scientific EZ-Link NHS-PEG4-Biotin). Blocking solution was removed and 100 μl/well of biotinylated mAb 1C7C7 ...
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bioRxiv - Microbiology 2019Quote: ... total RNA (100 ng) was converted to biotin-labeled cRNA with an Ambion Wild-Type Expression Kit (Life Technologies) and was hybridized to a Mouse Gene 2.0 ST GeneChip microarray (Affymetrix) ...
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bioRxiv - Genomics 2020Quote: ... the restriction fragment overhangs were filled in and the DNA ends were marked with biotin-14-dATP (Life Technologies). The biotin-labeled DNA was sheared and pulled down using Dynabeads MyOne Stretavidin T1 beads (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: Biotinylated sense (negative control) and antisense biotin-labeled DNA oligomers corresponding to GUARDIN (1μg) were coupled to streptavidin-coupled Dynabeads (Invitrogen). The Dynabeads were subsequently incubated with cell lysates for 4 h and eluted proteins subjected to SDS–PAGE and staining with Coomassie Brilliant Blue G-250 ...
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bioRxiv - Genetics 2021Quote: ... Newly synthesized transcripts were selected for biotin-16-UTP incorporation using streptavidin coated magnetic beads (ThermoFisher, Dynabeads M-280). Beads were extracted using QIAzol reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotin-labeled HNRNPH1 and AdML pre-mRNAs were transcribed with a MEGAscript T7 transcription kit (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... Biotin-labeled HNRNPH1 and AdML pre-mRNAs were transcribed with a MEGAscript T7 transcription kit (Invitrogen–Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2020Quote: ... intratesticular injection of EZ-Link Sulfo-NHS-LC-Biotin (15μl, 7.5mg/mL) was performed in the left testis (21335, Thermo Scientific). Testes were harvested 30 min after injection and fixed in 4% PFA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The liganded beads were blocked with excess biotin and washed with SEA BLOCK Blocking Buffer (Thermo Fisher, Rockford, IL), 1 % BSA ...
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bioRxiv - Bioengineering 2019Quote: ... was biotinylated using a 5:1 molar excess of Ez-Link™ Sulfo-NHS-LC-Biotin (Thermo-Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: 40-80 ng of each probe (labeled with biotin or Dig) and 1µg of unlabeled human COT1-DNA (Invitrogen) were dissolved in 4µl of hybridization buffer (10% dextran sulfate ...
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bioRxiv - Genomics 2021Quote: ... The secondary antibody staining and TSA reaction reagents were from a commercial Biotin-XX-Tyramide Superboost kit (Invitrogen, #2005939) and were used as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Glass slides were treated with 3-aminopropyl triethoxysilane and passivated with a biotin-PEG SVA/mPEG SVA mix (Invitrogen). Treated glass slides were assembled with double sided tape and an aluminum backing to generate flow channels in a custom microscope slide ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestion generated overhangs were filled in with a mixture of 0.4mM biotin-14-dCTP (10 mL; Thermo Fisher Scientific) and 10mM dATP/dGTP/DTTP (0.5 mL each ...
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bioRxiv - Neuroscience 2021Quote: ... then cells were placed on ice and incubated with 1.5 mg/ml Sulfo-NHS-SS-biotin (ThermoFisher Scientific, #21331) for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Cortical neuronal cultures were washed three times with ice-cold PBSCM (PBS + 1mM MgCl2 + 0.1 mM CaCl2) and incubated with 0.5 mg/ml Sulfo-NHS-SS-biotin (Life Technologies) for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Anti-mouse VE-cadherin (VC) antibody was biotinylated in-house using FluoReporter Mini-Biotin-XX Protein labeling kit (Invitrogen). Cell populations were sorted using a FACSAria-II sorter (BD ...
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bioRxiv - Genomics 2021Quote: ... 0.5μM oligo-dT (IDT; 100uM 5′-biotin-ACGAGCATCAGCAGCATACGA-T30VN-3′) and 0.5mM dNTPs/each (Thermo Fisher; 25 mM each) and snap frozen at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... or with Biotin-SP (1:100; Jackson Immuno Research) followed by fluorescent conjugates of streptavidin 647 (1:500; Invitrogen) in 0.1M DPBS ...
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bioRxiv - Cell Biology 2022Quote: ... EC were washed three-times with ice-cold PBS and incubated with 0.3 mg/ml EZ-link Sulfo-NHS-LC-Biotin (ThermoFisher) in PBS containing 2 mM MgCl2 and 2 mM CaCl2 on ice for 30 minutes with gentle agitation ...
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bioRxiv - Biophysics 2022Quote: ... Equal aliquots of RBCs were then mixed with various concentrations of EZ-Link Sulfo-NHS-LC-Biotin (Thermo Scientific) at a pH of 7.2 for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cell surface proteins were biotinylated with 0.5 mg/ml Ez-link sulfo-NHS-SS-Biotin (21331, Thermo Fisher Scientific) in PBS containing 1 mM CaCl2 and 0.5 mM MgCl2 (PBS/CM ...
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bioRxiv - Synthetic Biology 2022Quote: The RBDs were biotinylated by amine coupling with sulfo-NHS-SS-Biotin according to the manufacturer’s manual (Thermo Fisher). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 1 µl of a 12 mM solution of dextran (3kDa, tetramethylrhodamine and biotin labeled, D7162, Thermo Fisher Scientific) in sterile DMSO was injected using a pulled glass pipette (tip diameter ∼30 μm ...
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bioRxiv - Immunology 2022Quote: ... Equal aliquots of RBCs were then mixed with various concentrations of EZ-Link Sulfo-NHS-LC-Biotin (Thermo Scientific) at a pH of 7.2 for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells (5×106/0.5 ml) were labeled with 100 μg Immunopure Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) for 30 min at 22 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with biotin monoclonal antibody (BK-1/39) conjugated with Alexa Fluor 488 (AF488, Thermo Fisher Scientific), and imaged by Leica SP8 laser confocal microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA purification and enrichment of biotin-labeled DNA with Dynabeads™ M-280 streptavidin (Thermo Fisher Scientific, Cat# 11205D). The enriched bead-bound DNA was subjected to end repair ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin-16-UTP-labelled NEAT1_1 IVT product (1 µg) was immobilized on 25 µL streptavidin C1 dynabeads (Invitrogen), washed twice with 800 µL IP buffer and incubated (2 h ...
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bioRxiv - Microbiology 2024Quote: ... both RBD and HEL were site-specifically biotinylated in house using EZ-link maleimide-PEG2-biotin (ThermoFisher, CAT#A39261) so that the binding epitopes on these proteins were not modified ...
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bioRxiv - Cell Biology 2024Quote: ... 0,5µM Smartseq3 OligodT30VN (IDT; 5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’) adjusted to RT volume and 0,5mM dNTPs/each (Thermo Fisher, #R0181). After cell sorting lysis plates were centrifuged before storage at -80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with EZ-Link™ Sulfo-NHS-SS-Biotin (0.5 mg ml-1, #21331; Thermo Fisher) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 2 mM MgCl2 and 0.1 mM CaCl2 (PBSCM) and incubated in 0.5 mg/mL Sulfo-NHS-LC-Biotin (Thermo Fisher) dissolved in PBSCM at 4°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Signal was enhanced using a triple western blot protocol with biotin conjugated goat antirabbit IgG and Alexa Fluor 680 conjugated S-32358-streptavidin (1:100,000)35 and 31820-Goat anti-rabbit IgG (H+L) secondary antibody Biotin IgG (1:100,000) from Thermo Fisher Scientific were used.
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hSA4 antibody (in-house antibody) was generated using 200 μg Sulfo-NHS-LC Biotin (Pierce, Thermo Fisher Scientific), diluted 1:200 in blocking buffer and added to the plate for 1 hour at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked with 5% bovine serum albumin in TBST and biotin was detected with Streptavidin-HRP (ThermoFisher).
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bioRxiv - Immunology 2023Quote: ... cerevisiae was subjected to biotinylation in 1 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific #21331) for 1h at RT with rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... biotin-labeled oligonucleotide probes were used together with chemiluminescent nucleic acid detection kits according to manufacturer’s instructions (Thermo Scientific). The 18S RNA from the agarose gel run was used as loading control ...
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bioRxiv - Biophysics 2023Quote: ... Protein at 2 mg/mL in interaction buffer (150 mM NaCl 50 mM Phosphate pH 8) was treated with 1.25 mM Biotin LC-Hydrazide (Thermofisher) and 5 mM EDC for two hours then dialyzed against the initial buffer ...
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bioRxiv - Genomics 2023Quote: ... Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific, Cat # 65306) into DNA lo-bind tubes ...
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bioRxiv - Microbiology 2023Quote: ... a biotin-labeled 64-nt single-stranded DNA and the Century-Plus RNA ladder (Thermo Fisher Scientific, MA, USA) were loaded to an 8% polyacrylamide gel and electrophoresed in 1× TBE buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylation of the cell surface was performed by adding 1 mM Sulfo-NHS-LC-Biotin (Thermo Fisher, Waltham, MA) to the cell pellet at 250 μL per 1 million cells ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface proteins were subsequently labelled with 0.3 mg/ml EZ-Link™ Sulfo-NHS-SS-Biotin (Thermo Scientific) at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation overnight with biotin-XX goat anti-rabbit (1:500 in PBS, Invitrogen, Cat: B2770, LOT:1870403). To amplify the histamine signal ...
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bioRxiv - Genomics 2023Quote: ... Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific, Cat # 65306) into DNA lo-bind tubes ...
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bioRxiv - Genomics 2023Quote: Fragment ends were filled in by adding 37.5 μL of 0.4 mM biotin-14-dATP (ThermoFisher Scientific, # 19524-016), 1.5 μL each of 10 mM dCTP ...