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Citations for Thermo Fisher :
5251 - 5300 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and lysed for 15 min on ice using M-PER Mammalian Extraction Buffer containing Halt Phosphatase Inhibitor and EDTA-free Halt Protease Inhibitor Cocktail (1:100 each; Thermo Scientific). Lysates were centrifuged for 15 min at 16,000 × g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Serial 10-fold dilutions of gene fragments were performed in sheared salmon sperm DNA 5 µg mL-1 (m/v) (Thermofisher, LT) diluted in Tris-EDTA (TE ...
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bioRxiv - Plant Biology 2023Quote: ... cells were harvested and RNA was extracted using the Sigma Spectrum total RNA kit and cDNA was synthesized using M-MLV reverse transcriptase (Thermo Fisher). Quantitative reverse-transcription-PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). Samples were denatured in Laemmli buffer at 95 °C and run at 170 V on a 10% acrylamide gel with a 4% stacking gel ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were rinsed twice with PBS and lysed in 8 M urea 50 mM Tris pH 7.4 containing protease inhibitor (Thermo Fisher Scientific) and DTT ...
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bioRxiv - Cell Biology 2023Quote: ... Tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 µm resin (Waters) on an in-line 150 x 0.075 mm column using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific). Peptides were eluted using a 60 mins gradient from 98:2 to 65:35 buffer A:B ratio (Buffer A = 0.1% formic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... or normal IgG (4 µg) was incubated with 50 µl Dynabeads M-280 sheep anti-mouse IgG magnetic beads (Thermo Fisher Scientific Cat# 11201D ...
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bioRxiv - Developmental Biology 2023Quote: ... Each PLAC-seq library was prepared using DpnII as the restriction enzyme and Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen #11203D) mixed with 5 µg of H3K4me3 (04-745 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was sectioned at 20 µm on a cryostat at - 20°C and mounted directly onto SuperFrost Plus microscope slides (cat. no22-037-246, Fisher Scientific). Slides were air dried at room temperature for 24 hours before undergoing RNAScope in situ hybridization ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved at flow rate of 250 nL/min on an Easy-Spray 50cm X 75 m RSLC C18 column (Thermo Fisher), using a 123 min gradient of 3% to 35 % of Buffer B (0.1% FA in Acetonitrile ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were pelleted by centrifugation at 1,000 g for 5 min and lysed with M-PER™ mammalian protein extraction reagent (Thermo Scientific) supplemented with Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with DPBS and were pelleted by centrifugation at 1,000 g for 5 min and lysed with M-PER™ mammalian protein extraction reagent (Thermo Scientific) supplemented with the Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The calculated extinction coefficient of 28100 M-1cm-1 was used to measure the concentration using a NanoDrop 2000 (Thermo Fisher) spectrometer ...
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bioRxiv - Biophysics 2023Quote: ... was washed with buffer A (0.1 M sodium phosphate, 150 mM NaCl, 0.01% Tween20, pH 7.4) using DynaMag™-2 Magnet (Thermo Fisher Scientific, 12321D). After the wash ...
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bioRxiv - Cell Biology 2023Quote: ... was adjusted to a concentration of 1 mg/mL and then its buffer was exchanged to 0.1 M sodium bicarbonate using a Zeba spin desalting column (Thermo Scientific, 89882). For fluorescence labelling of gelatin or vitronectin ...
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Immunology 2023Quote: Whole cell and nuclear compartment were lysed with 8 M Urea buffer containing a protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) and were sonicated for 15 seconds at 60% amplitude ...
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bioRxiv - Microbiology 2023Quote: ... aliquots of 100 µl of collected NaOH samples were mixed with 100 µl of 1 M HCl (Fisher Scientific U.K. Ltd) and immediately covered with HCN detection filter paper (16) ...
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bioRxiv - Microbiology 2023Quote: ... samples were resuspended to a concentration of 1 µg/mL in 100% MeOH containing 2 µM sulfamethazine as an internal standard and LC-MS/MS analysis was performed using an UltiMate 3000 UPLC system (Thermo Scientific) with a Kinetex 1.7 µm C18 reversed phase UHPLC column (50 X 2.1 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were isolated at 6-hour intervals for 24 hours using CelLytic M lysis reagent (MilliporeSigma, Burlington, MA) and Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific). A total of 30 μg of each sample was loaded onto a Mini-PROTEAN TGX 7.5% precast mini-gel (Bio-RAD Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed for 5 minutes in 0.1 M Tris and mounted with coverglass in ProLong gold with DAPI (Invitrogen, Cat#P36931). Fluorescent slides were imaged at 20x magnification on a Zeiss AxioScan 7 microscope.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The chromatographic separation was achieved using a TR-5MS capillary column (30 m × 0.25 mm × 0.25 μm i.d.; Thermo Fisher Scientific, Cheshire, UK) with a helium carrier gas (99.999% purity ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 for 24 h before the cells were lysed with 300 µl M-PER Mammalian Protein Extraction Reagent (Thermo Scientific). A volume of 10 µl was resolved on a 3-8% Tris Acetate gel ...
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bioRxiv - Genomics 2023Quote: ... Macrophages were lysed 16 hours post-infection with a guanidine thiocyanate solution (4 M) plus β-mercaptoethanol and total RNA was isolated from the bacterial pellets using TRIzol reagent (Ambion) in the presence of zirconia/silica beads ...
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bioRxiv - Plant Biology 2023Quote: ... patens) or 1 µg (M. polymorpha) of DNase-treated total RNA was retro-transcribed with the SuperScript IV enzyme (ThermoFisher Scientific) and an oligo(dT)18 ...
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bioRxiv - Neuroscience 2023Quote: ... each hippocampal nuclei suspension was subjected to a 40-minute incubation with 30 µL pre-washed M-280 Streptavidin Dynabeads (#11205, ThermoFisher Scientific). The mixture of nuclei and beads in each sample was placed on a Dyna-2 magnetic stand (#12321.D ...
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bioRxiv - Neuroscience 2023Quote: ... The drill was then replaced with a micropipette loaded with 3-5nL of 0.6 M MnCl2 with 0.3mg/ml 3K rhodamine dextran amine (RDti and 0.15mg/ml 10K fluorescein dextran amine (Molecular Probes/Invitrogen/ThermoFisher) in sterile phosphate-buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... and a TR-FAME column (70% cyanopropyl polysilphenylene-siloxane; 120 m, 0.25 mm ID, 0,25 µm df, Thermo Scientific, Bremen, Germany). The samples were injected in splittless mode ...
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bioRxiv - Biophysics 2023Quote: ... The desired nominal Ca2+ concentration was achieved using WebMaxC to calculate the volume of a 1 M CaCl2 stock (Thermo fisher) in HEDTA (http://web.stanford.edu/~cpatton/webmaxcS.htm).
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bioRxiv - Microbiology 2023Quote: ... produced from hybridoma cell line supernatants and purified using protein A Sepharose) were bound to 5mg of M-280 Tosylactivated Dynabeads (Invitrogen # 14203) O/N at 37⁰C on a rotating wheel ...
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bioRxiv - Biochemistry 2023Quote: Samples were applied to glow discharged C-flat 1.2/1.3 μm Cu grids (Protochips, #M-CF313-100) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Scientific) at 4 °C and 100 % humidity.
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bioRxiv - Neuroscience 2023Quote: Fetal brains (within the skulls) and placenta were cryosectioned (300 µm thick) at −12 °C as before using M-1 embedding matrix (Thermo Scientific) (Tobiansky et al ...
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bioRxiv - Molecular Biology 2023Quote: ... or 90 pmol of SBP-tagged eIF4A1 protein was incubated with 30 μl of Dynabeads M-270 Streptavidin (Thermo Fisher Scientific), which had been preequilibrated with equilibration buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... cDNAs for qRCP were generated with 1 µg DNase treated total RNA samples using the M-MLV reverse transcriptase and random hexamer primers (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The reactions were stopped by adding 100 µL H2SO4 (0.5 M) to each well and absorbances were measured in a microplate reader (Multiskan FC; Thermo Fisher Scientific) at 450 nm and 620 nm.
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bioRxiv - Microbiology 2023Quote: ... a program with 6 repeats of bead beating at 4.5 m/sec was performed for 45 seconds using the FastPrep-24™ 5G (Thermo Fisher). Samples were incubated for two minutes on ice after the third repeat ...
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bioRxiv - Microbiology 2023Quote: ... 3 repeats of bead beating at 4.5 m/sec was performed for 45 seconds using the FastPrep-24™ 5G (Thermo Fisher) and the final elution volume was decreased to 40 μL.
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bioRxiv - Cancer Biology 2023Quote: Clear lysates from cells and EVs in 8M urea buffer (8 M urea, 75 mM NaCl, 50 mM Tris) were quantified using microBCA Protein Assay Reagent Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: SVEC4-10 cells were transfected with 20 nM siGENOME Nemo SMARTpool (Horizon Discovery #M-040796-01-0005) or siGENOME non-targeting control (Dharmacon #D0012061305) using lipofectamine RNAiMAX (Invitrogen #1377075). Experiments were performed 48 hours after transfection.
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bioRxiv - Cancer Biology 2023Quote: ... A predetermined volume of 1 M NaOH was quickly added and then the mixture was immediately plated in a 24-well flat bottom plate (Fisher Scientific) and allowed to solidify at room temperature before adding media and dissociating the gels from the sides of the well ...
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bioRxiv - Cancer Biology 2023Quote: ... Pellets were resuspended in 2 mL of 8 M urea (containing 50 mM HEPES, pH 8.5) and protein concentrations were measured by BCA assay (Thermo Fisher Scientific) before protease digestion ...
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bioRxiv - Biochemistry 2023Quote: ... The rest of the lysate was incubated with 50 μl of precleaned streptavidin beads (Invitrogen, Dynabeads™ M-280 Streptavidin #11206D) at room temperature for 2 hr.
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... The MTU1-mediated mitochondrial tRNA τm5s2U (m/z 398.0686) modification was detected using a Q Exactive mass spectrometer (Thermo Fisher Scientific) in full mass scan and positive ion mode ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized with isoflurane and perfused through the ascending aorta with 0.1 M phosphate buffer saline (PBS pH 7.4, Gibco, Waltham, MA, USA) plus heparin (7,500 USP units) ...
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bioRxiv - Biophysics 2024Quote: ... Pure α-Synuclein was characterized by C4 analytical RP-HPLC column (Higgins Analytical) and ESI-MS (M+H+) and yield was determined by Pierce BCA assay (Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... the lysis of 20 M cells was pre-cleared by incubation with 125 μL of MyOne C1 Streptavidin beads (Invitrogen, USA) at 45 °C for 30 min ...