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Citations for Macherey-Nagel GmbH :
1351 - 1400 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel) and quantified for determining the purity and concentration ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and flowers (Suppl. Table S1.1) with the NucleoSpin RNA Plant and Fungi Kit (Macherey-Nagel, USA), and checked for integrity by capillary electrophoresis using an Agilent (Santa Clara ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from the cells using the NucleoSpin RNA kit (Macherey-Nagel), and cDNA was prepared using ReverTra Ace RT Master Mix (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, 740609) and quantified by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted using Nucleozol (Macherey-Nagel), poly-A selected using oligo dT-beads (Dynabeads mRNA DIRECT Kit life tech) ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting constructs were used for protoplast transformation through midiprep using the NucleoBond Xtra plasmid kit (Macherey-Nagel GmbH & Co.KG, Duren, German) as per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... preparative HPLC was employed using a reversed-phase C18 column (Macherey-Nagel VP 250/21 Nucleodur 100-5) with a mobile phase solvent A (Water + 0.05% vol/vol trifluoroacetic acid (TFA)) ...
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bioRxiv - Neuroscience 2023Quote: ... coli transformed clones using the NucleoBond Xtra Midi Kit (Macherey-Nagel), according with the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using Nucleospin kit (Macherey-Nagel, Düren, Germany), and subcloned into pGEM-T plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified using Nucleospin kit according to manufacturer’s instructions (Macherey-Nagel, Düren, Germany), and visualized by electrophoresis using 2.5 % LE agarose gel ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: Minipreps were performed using NucleoSpin® Plasmid EasyPure Kits (Macherey-Nagel, Germany). 1 % agarose gels were run at 100 V for 20-30 min using 6x DNA Gel Loading Dye and GeneRuler 1 kb DNA Ladder (Thermo Fisher Scientific Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... and the resultant powder was transferred and redissolved in lysis buffer provided by the Nucleospin Plant II kit (Macherey-Nagel, Clonetech, CA, USA). Genome isolation was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA from the resulting immunopurified material was extracted using NucleoSpin RNA XS kit (Macherey-Nagel, 740902) in 100 μL RA1 lysis buffer and purified according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified using magnetic beads (NucleoMag NGS Clean-up and Size Select, Macherey-Nagel) according to the manufacturer’s instructions in a DNA to beads volume ratio of 1:1.8 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was purified using NucleoBond PC 2000 plasmid megaprep kit (Macherey-Nagel, #740549). Recombinant protein expression was carried out at the Protein Expression Laboratory ...
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bioRxiv - Microbiology 2023Quote: ... or the mucosal lining using a NucleoSpin RNA isolation kit (Macherey-Nagel), 0.5-1 μg was reverse transcribed into cDNA (SuperScript IV VILO Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... and 2.5 µl of RNAse (Macherey-Nagel) at 1000 unit and 3.5 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was purified with the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). The binding of cAMP-CRP (kindly provided by Annie Kolb ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Microbiology 2023Quote: ... Both products were gel-purified using the NucleoSpin Gel and PCR clean-up system (Macherey-Nagel) and stored in 5 mM Tris-HCl pH 8.5 at-30°C ...
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bioRxiv - Immunology 2023Quote: RNA extraction of in vitro cultured Th17 cells was performed with the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... PCR products were cleaned using PCR clean-up kit (Macherey-Nagel REF#740609) and validated after migration on BioAnalyser (Agilent) ...
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A systematic approach identifies p53-DREAM target genes associated with blood or brain abnormalitiesbioRxiv - Genetics 2023Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was extracted using the Nucleo-Spin RNA II kit (Macherey-Nagel). 1,000 ng of total RNA was used as input for cDNA synthesis using High- capacity cDNA Reverse Transcription kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... one of which was treated with 20 µg/mL of Proteinase K (740506.75, Macherey-Nagel), and incubated at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA of progeny virions was extracted from supernatants using the NucleoSpin RNA virus kit (Macherey-Nagel, #740956) according to the manufacturer’s instructions and stored at −80 °C until PCR-based analysis.
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bioRxiv - Cancer Biology 2023Quote: ... purified with NucleoSpin RNA columns (Macherey-Nagel), quantified by Nanodrop One (Thermo Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany); their concentration was brought to 30 ng/μl ...
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bioRxiv - Plant Biology 2023Quote: ... and total RNAs were extracted using Nucleospin RNA Plant (Macherey-Nagel). Five hundred ng of total RNAs were used for each sample ...
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bioRxiv - Neuroscience 2023Quote: ... RNA purification kit (Macherey-Nagel, #740902.50), SYBR Green reactifs (LightCycler® 480 SYBR Green I Master ...
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bioRxiv - Physiology 2023Quote: ... RNA from FAC-sorted lung EC was extracted using the Nucleaospin® TriPrep Kit (MACHEREY-NAGEL, 740966.50, Düren, Germany Germany). RNA quantity and quality were assessed using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The final PCR products were purified using a PCR clean-up kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
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bioRxiv - Genomics 2023Quote: ... Maxi kit for DNA from blood (Macherey-Nagel) and treated by DNase-free RNase A followed by ethanol precipitation ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
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bioRxiv - Genomics 2023Quote: NucleoSpin Blood kit (Macherey-Nagel, 740951.50) was used to isolate the genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: ... the medium in the reservoirs was collected and the cells from the chambers were lysed with 350 µl Lysis Buffer RA1 (Macherey-Nagel, Germany) supplemented with 3.5 µl ß-mercaptoethanol (Sigma ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was then extracted from homogenates using the NucleoSpin 96 Tissue Core Kit (Macherey-Nagel) and stored at −20°C until use.
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bioRxiv - Microbiology 2023Quote: ... silica gel G60 plates (10 x 20 cm, Macherey-Nagel) were used to spot samples and lipids were separated with CHCl3/CH3OH (90:10 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated using the NucleoSpin® RNA Plus XS kit (740990.50, Macherey-Nagel) or RNeasy Mini Kit (74104 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and plasmid kits were purchased from Macherey-Nagel. Precision Plus ProteinTM protein standard was provided by Biorad ...
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bioRxiv - Synthetic Biology 2023Quote: ... in Protino gravity columns (Macherey-Nagel), previously equilibrated with 2 column volumes of buffer A ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA purification from cultured cells and tissue (740955; Macherey-Nagel). Complementary cDNA was synthesised using Maxima first strand cDNA kit from five hundred nanograms of purified total RNA (K1641 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The pooled extracts were further desalted and purified on a Chromabond® C18 ec poplyprolyene column (Macherey-Nagel, Düren, Germany). The extracted glycolipids were evaporated to dryness under nitrogen gas to remove organic solvents for deglycosylation ...
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bioRxiv - Physiology 2023Quote: ... The mixture was dried under a stream of N2 (g) and samples were resuspended in methanol (140 μL) and transferred into a running vial (Macherey-Nagel, Hoerdt, France). The mixture was then dried and resuspended in methanol (10 µL ...