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Citations for Applied Biological Materials :
151 - 200 of 432 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The cell line identities were confirmed by genotyping using short tandem repeat fingerprinting and were tested negative for mycoplasma with Mycoplasma PCR Detection Kit (ABM).
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bioRxiv - Systems Biology 2022Quote: ... Cells were regularly passaged and tested for presence of mycoplasma contamination (Mycoplasma PCR Detection Kit, Applied Biological Materials).
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bioRxiv - Genetics 2022Quote: ... Lentiviral shRNA particles were titered using the qPCR Lentiviral Titration Kit (ABM). shRNA particles were transduced into 12Z cells at a 100-fold multiplicity of infection ...
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bioRxiv - Molecular Biology 2022Quote: ... an anti-His rabbit polyclonal antibody (ABM, Richland, BC, Canada) in combination with a polyclonal goat anti-rabbit HRP conjugated secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with 1μg/mL of mouse anti-Flag (Applied Biological Materials Inc., Richmond, BC, Canada), 0.25 μg/mL of rabbit anti-SARS-CoV-2-Nsp1 (Genetex ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentivirus titer was measured using a Lentivirus qPCR Titer kit (Applied Biological Materials, Richmond, BC, Canada).
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bioRxiv - Bioengineering 2022Quote: ... Cells were seeded in uncoated flasks and maintained in astrocyte basal medium (ABM) supplemented with rhEGF ...
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bioRxiv - Microbiology 2022Quote: We built an agent based model (ABM) of cell growth and division ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we briefly describe the agent-based model (ABM) implemented in Julia (varmodel3 ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed to generate cDNA using 5X All-In-One RT MasterMix (with AccuRT Ge5X All-In-One RT MasterMix and AccuRT Genomic DNA Removal Kit) (Applied Biological Materials) for RT–qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... Mycoplasma testing was conducted monthly using a mycoplasma PCR detection kit (ABM, G238). Human and mouse cell lines were authenticated by STR profiling (Almeida et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... three different CRISPR/Cas9 sgRNA designs in an all-in-one lentiviral vector (ABM, 382541140595) were purchased ...
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bioRxiv - Cancer Biology 2022Quote: ... with an additional modification of a deletion of 1 adenosine in the 10-A polynucleotide sequence in exon 10 (codons 771-774) of the ATR cDNA sequence (Applied Biological Materials).
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bioRxiv - Cancer Biology 2022Quote: ... A GFP tag was added by Applied Biological Materials (Richmond, BC). ATR-I774Yfs*5-GFP is a modification of the ATR-WT-GFP PCDNA 3.1 plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ZNF692 sgRNA CRISPR/Cas9 All-in-One Lentivector or Scrambled sgRNA CRISPR/Cas9 All-in-One Lentivector (Applied Biological Materials) was transfected together with lentiviral packaging plasmids pSPAX2 and pMD2g to HEK293FT cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription was performed using All-in-One 5X RT MasterMix (ABM, G592). Primer Express 3.0 software was used to design primers and qRT-PCR was performed as previously described (83,84 ...
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bioRxiv - Microbiology 2022Quote: ... Genomes were quantified using a qPCR Lentivirus Titer Kit (Applied Biological Materials, LC900) as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... the PCR products and digested plasmids were fused into complete expression plasmids using the Ligation-Free Cloning System (Applied Biological Materials, Inc., Canada).
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bioRxiv - Genetics 2022Quote: The SMN1 AAV Vector (Human; hSyn; Luc) (#444551011210) containing the functional, synthetic SMN1 gene (GenBank accession code, NM_000344) was obtained from Applied Biological Materials Inc ...
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bioRxiv - Immunology 2022Quote: ... cDNA was obtained using RT MasterMix (Applied Biological Materials) and used to perform a SYBR-based real-time PCR (BioLine ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral DNA constructs were packaged using ABMgood packaging mix (Applied Biological Materials Cat. no. LV053) transfected into 293 FT cell lines using jetPRIME transfection reagent (Polyplus ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral titers were obtained using ABMgood qPCR Lentivirus Titer Kit (Applied Biological Materials, Cat. no. LV900). All vectors were confirmed by restriction digest and Sanger sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... and human recombinant bFGF-2 (10ng/mL; ABM). DMEM-F12 w/o L-Glutamine w/o Hepes w/o Glucose (Biowest ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL; ABM), and human recombinant bFGF-2 (10ng/mL ...
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bioRxiv - Cell Biology 2022Quote: ... lentiviral particles were titrated using the qPCR lentivirus titration kit (Applied Biological Materials Inc.) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and Lin28 (Cat#: G353, ABM Inc., Canada). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... EEO media was defined as the minimal set of components needed to establish organoid cultures expansion and was composed of advanced basal media cocktail10 (ABM, 1X); rhEGF (50 ng/mL ...
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bioRxiv - Microbiology 2022Quote: ... Cells were routinely confirmed to be mycoplasma negative according to PCR Mycoplasma Detection Kit (ABM Inc.) and were used at low passage (<10 ...
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bioRxiv - Cancer Biology 2022Quote: ... pLenti-III HSVtk Lentivirus vector was purchased from Applied Biological Materials (abm).
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bioRxiv - Microbiology 2022Quote: ... 3,3’,5,5’-Tetramethylbenzidine (TMB) solution was purchased from Applied Biological materials (ABM), Canada ...
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bioRxiv - Microbiology 2022Quote: ... solution was purchased from Applied Biological materials (ABM), Canada ...
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bioRxiv - Microbiology 2022Quote: We constructed an agent-based model (ABM) in the program Netlogo (66 ...
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bioRxiv - Immunology 2022Quote: ... One quarter of the total RNA sample was used as template for OneStep RT-PCR (ABM) using the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... pshPVR (ABM #i018439) for shRNA or pHAGE-Scr or pHAGE-UL141 for heterogenous expression ...
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bioRxiv - Neuroscience 2022Quote: ... The titer was determined using the qPCR Adeno-Associated Virus Titration kit (Applied Biological Materials) and the purity was verified by SDS-PAGE and total protein staining using instant blue reagent (Expedeon) ...
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bioRxiv - Microbiology 2022Quote: ... BlasTaq 2X qPCR MasterMix (G891, Applied Biological Materials Inc) and ABI Step One system (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized from 1 μg RNA by using the OneScript® Plus cDNA Synthesis Kit (Applied Biological Materials Inc.). Gene-specific primers pairs (Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... COL-hTERT cells (T0570) were purchased from ABM (Richmond, BC). 293 Flp-In cells were obtained from Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... the parental HeLa cell lines were authenticated by genomic sequencing of 24 loci (University of Utah Sequencing Core) and confirmed to be mycoplasma-free by routine PCR testing (ABM) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: hTERT immortalized fetal astrocyte cell lines were supplied by Applied biological materials (Abm) (#T0281) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lentivirus titer determined using the qPCR lentivirus titer kit was ~108 IU/mL (Applied Biological Materials).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in DMEM supplemented with 10% FBS and 100 units/mL penicillin/streptomycin under 5% CO2 at 37C and were routinely tested for mycoplasma contamination by ABM’s PCR Mycoplasma detection kit ...
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bioRxiv - Cell Biology 2022Quote: ... or scrambled siRNA GFP lentivector (Applied Biological Materials, catalog #: LV015-G) together with psPAX2 and pMD2.G plasmids using TransIT-2020 ...
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bioRxiv - Cancer Biology 2022Quote: ... Human HC cells immortalized by human telomerase reverse transcriptase (hTERT) expression were purchased from Applied Biological Materials (cat. no. T0570). The cell lines used were not further authenticated ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transfected with a Hsp47-set of four siRNA lentivectors (rat) (Applied Biological Materials, catalog #: 435050960395), or scrambled siRNA GFP lentivector (Applied Biological Materials ...
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bioRxiv - Cell Biology 2022Quote: ... The lentivirus was quantified with the qPCR lentivirus titration kit (Applied Biological Materials, catalog #: LV900), and stored at −80°C.
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bioRxiv - Cell Biology 2022Quote: ... Scrambled siRNA GFP lentivector (catalog #: LV015-G) and Hsp47-set of four siRNA lentivectors (rat) (catalog #: 435050960395) were obtained from Applied Biological Materials. psPAX2 (Addgene plasmid # 12260 ...
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bioRxiv - Cancer Biology 2022Quote: ... ctDNA was ligated to the PACBIO adaptor (GCGCTCTGTGTGCT) using the ABM DNA Library Prep Kit for Illumina Sequencing (Applied Biological Materials Inc. Canada). PACBio-labeled ctDNA and regular ctDNA were subjected to standard methods for library preparation and sequencing using Illumina and Agilent protocols ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was converted into cDNA using 5x ALL-In-One RT Master Mix (Applied Biological Materials Inc, G490) following the manufacturers’ instructions and diluted 20 times with nuclease-free H2O ...
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bioRxiv - Developmental Biology 2022Quote: ... and sent to Applied Biological Materials (ABM) for Illumina sequencing ...