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Citations for Applied Biological Materials :
151 - 200 of 494 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Viral titers were assessed with a qPCR Lentivirus Titration Kit (ABM) and functional titers were assessed by transducing iPSC-derived NPCs with serial dilutions of virus and quantifying BFP fluorescence intensity and RNase H1 protein levels by Western Blot.
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bioRxiv - Immunology 2023Quote: ... pLenti-mCherry (derived from pLenti-III-EF1a, Applied Biological Materials Inc., LV043), and Lenti-luciferase-P2A-Neo (RRID:Addgene_105621).
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus particles expressing human α-syn under cytomegalovirus (CMV) promoter (Applied Biological Materials, Inc, Canada) was used to re-express α-syn in SK-MEL-28 SNCA-KO cells as described previously27
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bioRxiv - Immunology 2023Quote: ... The titer was determined using the qPCR Adeno-Associated Virus Titration kit (Applied Biological Materials) and the purity was verified by SDS–PAGE and total protein staining using InstantBlue reagent (Expedeon).
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bioRxiv - Synthetic Biology 2023Quote: ... All fragments were joined together by Gibson Assembly (mCherry-LOV-ABM). Variants with L514K and L531E mutations on the AsLOV2 domain were designed similarly64 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were screened for mycoplasma contamination using the Mycoplasma PCR Detection Kit (Applied Biological Materials, Inc.).
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was then synthesized using All-In-One RT MasterMix (Applied Biological Materials). qPCR was carried out using TB Green Premix Ex Taq II (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA (Applied Biological Materials, G166,1:4000), anti-VPS4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were stained with primary antibodies for 16 hours at 4 °C using the following antibodies: anti-GFP (Applied Biological Materials, G096, 1:1000), anti mCherry (novus biologicals ...
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bioRxiv - Microbiology 2023Quote: ... and the gel was stained with SybrGreen fluorochrome mixture (Safe-Green &Trade, ABM Inc. USA). The resulting bands were visualized with a Mini Blue light transilluminator (Cleaver Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... this computational model is an agent-based model (ABM) with bacteria ...
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bioRxiv - Cancer Biology 2023Quote: ... in a pLenti-Puro vector (pLenti-All-in-one-U6-sgRNA human Zeb1 (target1) or scramble -SFFV-Cas9 nuclease-2A-Puro) (Applied Biological Materials Inc., Richmond, Canada). The sequences of the sgRNA targeting ZEB1 are the following ...
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bioRxiv - Microbiology 2023Quote: ... Pseudomonas syringae Lz4W cells were grown in Antarctic Bacterial medium (ABM: 5g liter-1 peptone and 2g liter-1 yeast extract ...
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bioRxiv - Molecular Biology 2023Quote: ... hES cells were routinely tested for Mycoplasma using the Mycoplasma PCR Detection kit (Applied Biological Materials, Richmond, Canada).
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bioRxiv - Microbiology 2023Quote: ... using 2.5 U of Precision Taq Polymerase (Applied Biological Materials Inc, Richmond), each primer had a final concentration of 500 nM ...
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bioRxiv - Microbiology 2023Quote: ... 2.5 U of Precision Taq Polymerase (Applied Biological Materials Inc, Richmond). Each primer had a final concentration of 500 nM ...
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bioRxiv - Immunology 2023Quote: ... and human endometrial stromal cells (hESCs; T0533 ABM, Canada) were utilized in this study ...
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bioRxiv - Immunology 2023Quote: ... ready-to-use lentivirus particles containing three unique sgRNA targeting human Itch or viruses containing scrambled sgRNA were purchased from Applied Biological Materials Inc ...
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bioRxiv - Microbiology 2023Quote: ... The viral titer of concentrated viruses was calculated using the qPCR Lentivirus Titration Kit from ABM (Applied Biological Materials, Vancouver, Canada) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The viral titer of concentrated viruses was calculated using the qPCR Lentivirus Titration Kit from ABM (Applied Biological Materials ...
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bioRxiv - Bioengineering 2023Quote: Swan71 trophoblast cells (Applied Biological Materials Inc. T0532) were cultured on T25 flasks in phenol red-free DMEM/F12 (Gibco 21041-025 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were tested for mycoplasma using PCR-based screening (PCR Mycoplasma Detection Kit, Cat# G238, Applied Biological Materials Inc.) weekly or biweekly.
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bioRxiv - Cancer Biology 2023Quote: ... Concentrations of viral particles were verified via qPCR using a lentiviral titration kit from Applied Biological Materials on QuantStudio 3 from Thermo Fisher.
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bioRxiv - Cell Biology 2023Quote: ... containing 1ug of each of the gRNA and donor plasmid vectors (ABM, Richmond, Canada). Antibiotic selection was performed ...
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bioRxiv - Cell Biology 2023Quote: ... MuTuDC1940 cells were purchased from Applied Biological Materials Inc ...
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bioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed in serum free RPMI-1460 (w/o antibiotic and antimycotic) in the presence of ViralEntry™ Transduction Enhancer (Applied Biological Materials Inc. ABM, BC, Canada). A non-effective shRNA cassette in the TR30030 pRFP-C-shLenti vector plasmid was used as a scrambled control ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... and the HRP signal was visualized with ECL Western Blotting Detection reagents (ABM) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Human KCNJ3-HA plasmid was purchased from Applied Biological Materials (Richmond ...
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bioRxiv - Immunology 2023Quote: ... The lentivirus titer determined using the qPCR lentivirus titer kit was >109 IU/mL (Applied Biological Materials).
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bioRxiv - Microbiology 2023Quote: ... and co-transfected with recombinant saCas9 Nuclease NLS Protein (Applied Biological Materials Inc.), and a PCR-based puromycin resistance construct containing 5’- and 3’-UTRs of the targeted gene ...
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bioRxiv - Molecular Biology 2023Quote: A lentiviral vector (pLenti-U6-sgRNA-PGK-Neo) encoding the designed gRNA was synthesized by Applied Biological Materials Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... cultures were screened for various Mycoplasma strains using the PCR Mycoplasma detection kit (ABM) and confirmed negative before being used for experimental assays ...
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bioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed in serum free RPMI-1460 (w/o antibiotic and antimycotic) in the presence of ViralEntry™ Transduction Enhancer (Applied Biological Materials Inc. ABM, BC, Canada). A non-effective shRNA cassette in the TR30030 pRFP-C-shLenti vector plasmid was used as a scrambled control ...
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bioRxiv - Cell Biology 2023Quote: ... (ABM).
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bioRxiv - Biochemistry 2023Quote: ... anti-HA Tag (Applied Biological Materials, #G166; 1:1000), anti-G6PD (Abcam ...
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bioRxiv - Biochemistry 2023Quote: The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
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bioRxiv - Cell Biology 2023Quote: ... HaCaT cells were transfected with pLenti-EF1a-Cas9-Puro lentiviral particles (Applied Biological Materials, Richmond, Canada) and selected with 1 µg/mL puromycin (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized by reverse transcription using All-In-One 5X RT Master Mix (ABM-G592) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR reaction mixtures (25 μl) consisted of 12.5 μl of MegaFi Fidelity 2X PCR MasterMix (ABM Inc., Richmond, Canada), 1.5 μl of each primer (final concentration 0.6 μM) ...
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bioRxiv - Physiology 2023Quote: ... RNA samples underwent reverse transcription (All-in-one RT Master Mix – ABM). qPCR was performed with BrightGreen Express reagent (ABM ...
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bioRxiv - Physiology 2023Quote: ... qPCR was performed with BrightGreen Express reagent (ABM) and run on the CFX384 Real-Time PCR system for 40 cycles ...
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bioRxiv - Physiology 2023Quote: ... Coronary Arterial Smooth Muscle cells (CSM; Applied Biological Materials T0557) were propagated and prepared in the same manner ...
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bioRxiv - Genetics 2023Quote: ... Lentivirus was titered using the qPCR Lentivirus Titration Kit (Applied Biological Materials LV900).
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids with sgRNA target sequences cloned into pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro vector were obtained from ABM Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... Scrambled sgRNA vector was used as negative control (ABM Inc., K010)
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bioRxiv - Cancer Biology 2023Quote: U-87 MG and U-251 MG GBM cell lines were obtained from ATCC and tested to be mycoplasma free using the Mycoplasma PCR Detection Kit (Applied Biological Materials Inc; G238), prior to experimentation ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus particles expressing human α-syn under cytomegalovirus (CMV) promoter (Applied Biological Materials, Inc, Canada) was used to re-express α-syn in SK-MEL-28 KO cells as described previously30 ...
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bioRxiv - Cell Biology 2023Quote: ... Mycoplasma negativity of the original cell lines (hTERT RPE-1 and 293T) grown in antibiotics-free media were confirmed by a PCR based test (G238, Applied Biological Materials).
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bioRxiv - Cell Biology 2023Quote: ... miRNA Mimic Negative Control (ABM-MCH00000, abm), and Anti-miR™ miRNA Inhibitor (AM17000 ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were routinely tested for Mycoplasma via PCR test (Applied Biological Materials).