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Citations for Applied Biological Materials :
451 - 494 of 494 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... CA).19 Polymerase Chain Reaction was performed using qPCR master-mix (Applied Biological Materials Inc., Canada). The primer sequences are listed in Table 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lines used were regularly tested for mycoplasma contamination using a PCR Mycoplasma Detection Kit (Applied Biological Materials, G238).
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bioRxiv - Molecular Biology 2020Quote: cDNA synthesis was carried out with isolated total RNA using 5 X All-In-One RT MasterMix kit (Applied Biological Materials, G490) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequent qPCR analysis was performed with BrightGreen 2x qPCR Master mix (Applied Biological Materials Inc.) on a Bio-Rad 1000 Series Thermal Cycling CFX96 Optical Reaction module ...
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bioRxiv - Neuroscience 2020Quote: ... Virus titre was quantified using the qPCR Lentivirus Titration Kit according to manufacturer’s directions (Applied Biological Materials, British Columbia, Canada).
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bioRxiv - Molecular Biology 2020Quote: ... pilenti-SPINK1-shRNA-GFP-Puro construct(rat) from Applied Biological Materials(abm), Nanjing.
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bioRxiv - Microbiology 2020Quote: ... containing SafeView (ABM), and UV visualization were performed on Gel Documentation System WGD-30 (DAIHAN Scientific Korean) ...
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bioRxiv - Neuroscience 2020Quote: ... of AAV1 viral particles containing Tet1 isoform TALE constructs were packaged by Applied Biological Materials (ABM). For primary hippocampal neuron experiments in 12 or 24 well plates ...
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bioRxiv - Cell Biology 2020Quote: E14.5 FL and adult BM (ABM) cells were depleted for Ter119+ cells and lineage-positive cells ...
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bioRxiv - Genomics 2020Quote: ... twelve hours after blood meal (ABM), twenty-four hours ABM ...
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bioRxiv - Genomics 2020Quote: ... A group of males (blood fed, five days ABM) was dissected to obtain the testicles ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Lentiviruses were produced from sets of four mouse piLenti-siRNA-GFP lentiviral vectors (Applied Biological Materials, Inc. Richmond, BC, Canada) targeting Pelp1 (cat no ...
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bioRxiv - Immunology 2020Quote: Lentiviral plasmids encoding shRNAs were obtained from Sigma-Aldrich and all-in-one vectors carrying CTNNB1 sgRNA/Cas9 with GFP reporter were obtained from Applied Biological Materials. Each plasmid was transformed into One Shot® Stbl3™ chemically competent cells (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For all experiments human embryonic kidney (HEK) 293SL cells84 were used and regularly tested for mycoplasma contamination (PCR Mycoplasma Detection kit, ABM, Canada). HEK293SL cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Bioengineering 2020Quote: ... by following the procedures described elsewhere.55 PCR products were purified using DNA purification solid-phase reversible immobilization magnetic beads (G95, Applied Biological Materials Inc.) and the purified products were sent out for Sanger sequencing (Genewiz Inc.).
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bioRxiv - Genetics 2020Quote: ... Primary cell cultures were tested monthly for mycoplasma using a PCR Mycoplasma Detection Kit (Applied Biological Materials, Richmond, BC, Canada).
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bioRxiv - Genetics 2020Quote: ... and human recombinant bFGF-2 (10ng/mL; ABM). Cells were monitored for mycoplasma contamination using MycoAlert (Lonza ...
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bioRxiv - Genetics 2020Quote: ... human recombinant EGF (20 ng/mL; ABM, Richmond, BC, Canada) and human recombinant bFGF-2 (10ng/mL ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... carried out with OneScript® Plus cDNA Synthesis Kit (ABM), according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... carried out with the OneScript® Plus cDNA Synthesis Kit (ABM, Richmond, Canada). For amplification of the full HhTAR1 open reading frame (ORF) ...
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bioRxiv - Immunology 2020Quote: ... Cells were confirmed to be Mycoplasma-free using a PCR Mycoplasma Detection Kit (Applied Biological Materials Inc. G238). Cells were cultured in a 37°C humidified incubator containing 5% CO2.
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bioRxiv - Cancer Biology 2020Quote: ... SC-1800 cells were grown in astrocyte basal medium (ABM) supplemented with astrocyte single Quots™ (AGM-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using BrightGreen 2X qPCR MasterMix (ABM, Vancouver, Canada) on a QuantStudio 3 real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was then generated using the 5X RT Mastermix (ABM, Vancouver, Canada). Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using BrightGreen 2X qPCR MasterMix (ABM ...
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bioRxiv - Genetics 2020Quote: ... The target genes were detected using a Brightgreen 2× qPCR Mastermix low-rox (#Mastermix-lr; ABM.). The details of the primers used in these assays are given in Supplementary Table 1 in the Supplementary Information.
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bioRxiv - Evolutionary Biology 2020Quote: ... phenotypic distribution of available partners and of competitors will not affect their decision (i.e. no adaptive behaviour ABM wise), it only affects the outcome of interactions.
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bioRxiv - Evolutionary Biology 2020Quote: ... We use hereafter the term “demo-genetic agent-based model” (DG-ABM) to indicate that the present model integrates the full retroactive loops between these elements.
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bioRxiv - Cancer Biology 2020Quote: ... and the absence of mycoplasma was confirmed using Mycoplasma PCR detection kit (Applied Biological Materials).
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bioRxiv - Cell Biology 2020Quote: Cells were transduced by centrifugation (Xin et al., 2003) with DKK3 siRNA/shRNAi Lentivirus (iV006166) or scrambled siRNA GFP Lentivirus (LVP015-G) (Applied Biological Materials Inc., CA) and selected by passaging at least once into media containing 1–3 μg/mL puromycin until all cells were GFP+ before plating into Matrigel.
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Ex vivo Immune Profiling in Patient Blood enables Quantification of innate immune Effector FunctionsbioRxiv - Immunology 2020Quote: In order to investigate also spatial aspects of host-pathogen interactions we applied a previously developed agent-based model (ABM) [49,50] that was adjusted to the context of whole-blood infection assays [17,18] ...
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bioRxiv - Physiology 2020Quote: ... were cultured in astocytic basal medium (ABM) supplemented with AGM bulletKit™ (Lonza) ...
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bioRxiv - Physiology 2020Quote: ... and audible baseline monitor (model ABM-3, WPI) as previously described27 ...
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bioRxiv - Molecular Biology 2020Quote: ... Human colonic cells (hCC; T0570, Applied biological materials, Inc.) were cultured in DMEM supplemented with 10% FBS in 5% CO2/air humidified atmosphere at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Trans Genic Inc.) and HRP-conjugated goat anti-rabbit antibody (1:15 000 dilution, SH012; Applied Biological Materials). Chemiluminescence was detected using SuperSignal West Femto Max Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... the First-strand cDNA was synthesized using a 5X All-In-One RT MasterMix (ABM, Canada). The relative quantification was done by ChamQTM Universal SYBR® qPCR Master Mix (Vazyme ...
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bioRxiv - Microbiology 2020Quote: ... prior to cDNA synthesis using the 5X All-In-One Master mix (Applied Biological Materials), both according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 2 ug of RNA was treated with an AccuRT Genomic DNA Removal Kit (Applied Biological Materials, Richmond, BC, Canada) prior to cDNA synthesis using the 5X All-In-One Master mix (Applied Biological Materials) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant adenovirus expressing human ACE2 (Ad5-hACE2) and control adenovirus (Ad5-Ctrl) were purchased from ABM (Vancouver, Canada).
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bioRxiv - Microbiology 2020Quote: The recombinant adenovirus 5 expressing human ACE2 (Ad5-hACE2) and control adenovirus (Ad5-Ctrl) were purchased from ABM (Vancouver, Canada) or generated in the laboratory ...
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bioRxiv - Microbiology 2020Quote: The mAb 3D11-expressing hybridoma cell line variable heavy and light chain antibody genes were sequenced (Applied Biological Materials Inc). mAb 3D11 VK and VH regions were cloned individually into custom pcDNA3.4 expression vectors immediately upstream of human Igκ and Igγ1-CH1 domains ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA fragment length between 200-300bp were selected with SPRI magnetic beads (ABM, Richmond, BC, Canada), followed by bisulfite treatment (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... comprised of randomly ordered 30-second blocks of either cued AM retrieval (remembering previous autobiographical memories - ABM) or a 2-back WM task (remembering which word they saw 2 words ago in a stream of words) ...
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bioRxiv - Neuroscience 2020Quote: ... up to a maximum of 64 blocks (32 ABM, 32 WM), in chunks of 16 blocks ...
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bioRxiv - Neuroscience 2020Quote: ... Each block is either an autobiographical memory retrieval task (ABM task) or a 2-back working memory (WM ...