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Citations for Bio-Rad :
4851 - 4900 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Detection of proteins was achieved following 5 min incubation with Enhanced Chemiluminescence (ECL) reagent and exposed using a ChemiDoc imaging system (BioRad). Loading control was performed by normalizing the target protein to the stain-free images of total protein ...
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bioRxiv - Biophysics 2023Quote: ... The blots were finally washed thrice with TBST with 5 minute incubations before adding the ECL substrate solution (1705061, Biorad). Chemiluminescence images were acquired on a Chemidoc™ imaging system (Biorad) ...
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bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
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bioRxiv - Microbiology 2023Quote: ... followed by immunoprecipitation with 5 µg appropriate antibodies or corresponding IgG control using magnetic protein A/G beads (BIO-RAD). After several rounds of washing with low-salt wash buffer (20 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 10 minutes with 1x TBST solution followed by 5 minutes incubation at room temperature with either Clarity Western ECL (BioRad). Immunoblots were developed using an Amersham ImageQuant 500 CCD imaging system (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were boiled at 95 °C for 5 min before being loaded onto a polyacrylamide gel (Cat# 4561094, Bio-Rad). Proteins were blotted onto a PVDF membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies to the membrane for 1 h in the presence of 5% milk followed by four washes in TBS-T and developing with ECL substrate (1705061; BioRad). Blot signal was captured using the same BioRad ChemiDoc™ Touch Gel Imager ...
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bioRxiv - Microbiology 2023Quote: ... All samples collected were heated to 95°C for 5 minutes before resolving on a 12% mini-PROTEAN precast gel (BioRad). A Western blot using polyclonal EssR antiserum was conducted following the protocol outlined in the Western blot method section below ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
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bioRxiv - Genetics 2022Quote: ... Approximately 43µl of the newly formed droplet solution were transferred into a ddPCR 96-well plate and then sealed at 180°C for 5 seconds in the PX1 PCR Plate Sealer (BioRad). PCR was carried out with the following conditions ...
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bioRxiv - Plant Biology 2022Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
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bioRxiv - Microbiology 2022Quote: The apparent KD of GST-DnaA for the dnaX-ebfC 5’ UTR DNA probe was determined by densitometry from triplicate EMSAs using ImageLab software (BioRad). Lanes were manually set to the EMSA gel ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was washed for 5 min with TBS-T and then imaged using a ChemiDoc XRS+System (Bio-Rad). For chemiluminescence-based detection of immunoblots ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 15 μg siRNA (5 μg from each siRNA) was transferred to a 4-mm cuvette (Bio-Rad) and 5-10×106 DCs were added in 200 μl OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein concentrations of the supernatants were determined using DC™ Protein Assay Kit (Bio-Rad #5 000 112). Equal concentrations of protein (30 μg ...
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bioRxiv - Genetics 2023Quote: ... The beads were washed four times for 5 min with RIP buffer containing 0.5% sodium deoxycholate and boiled in 1xLaemmli Sample Buffer (Bio-Rad) for 10 min at 100°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 μl of the sample were heated at 95°C for 5 min and resuspended in 4x Laemmli sample buffer (BioRad)/50 mM DTT before SDS-PAGE.
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bioRxiv - Cancer Biology 2022Quote: ... 10-15 μg of protein samples and 3-5 μL of Precision Plus Protein Dual Color Standards (BIO-RAD, #1610374) were loaded in NuPAGE 4-12% Bis-Tris protein gels (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... bovis BCG-mCherry) respectively at an interval of 24 hours daily for 5 days using a fluorescence multimode plate reader (Biorad). An MOI of 1 ...
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bioRxiv - Microbiology 2023Quote: ... followed by three-times washing with TBST for 5 min and subsequent incubation with the goat anti-rabbit IgG (H+L)-HRP conjugate (BioRad) (diluted 1:3000 in TBST ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µg of protein was boiled for 5 min at 95°C and then loaded on a 4-15% SDS gel (Biorad).
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bioRxiv - Genetics 2024Quote: cDNA was generated using 5 ng of total RNA using iScript Reverse Transcriptase supermix (cat#1708841) and C1000 Touch Thermal Cycler (Biorad) as per manufacturer guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3 times for 5 min with 1X TBS 0.1% Tween 20 and unsaturated fluorescent signal was acquired using Chemidoc MP imager (Bio-Rad) with identical settings for samples to be compared within an experiment.
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bioRxiv - Cancer Biology 2024Quote: ... and RT-qPCR performed on the resulting cDNA (diluted 5-fold) using the iQ SYBR Green Supermix (Bio-Rad, #1708882) and following the manufactures instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed again after secondary antibody incubation and developed using either nitroblue tetrazolium (NBT)–5-bromo-4-chloro-3-indolylphosphate (BCIP) for AP-conjugated secondary antibodies or the ECL substrate (BioRad) for the HRP labelled secondary antibody after incubating for about 10 to 15 mins at room temperature with gentle shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... USA) in 5% (w/v) DifcoTM skim milk and developed using the ChemiDoc XRS+ imager (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Systems Biology 2024Quote: ... beads were resuspended in 1 mL of washing buffer (50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM EDTA) and transferred to BioSpin membrane columns (Bio-Rad). Beads were washed 3x with 1 mL washing buffer ...
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bioRxiv - Biochemistry 2024Quote: ... at a 20-90°C gradient (increment: 0.3 °C, hold for 5 s before read) on a Real-time PCR system (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed 3x with 1x TBST then incubated with ECL Prime (Amersham) for 5 minutes and imaged using a Chemidoc Imaging system (BioRad).
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bioRxiv - Microbiology 2024Quote: ... and 0.25µM of each 5’ 6-FAM/ZEN/3’ IBFQ or 5’ HEX/ZEN/3’ IBFQ probe with the QX200 Droplet Digital PCR System (Biorad). Reactions were performed using the following PCR cycle settings ...
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bioRxiv - Microbiology 2024Quote: ... 3-5 µL of cell suspension was then deposited on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) pad prepared using a gene frame positioned on a glass slide ...
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bioRxiv - Microbiology 2024Quote: ... boiled for 5 min at 95°C and the proteins were separated on 10–20% gradient polyacrylamide gels (Criterion precast gel, Biorad). The virus proteins were visualised by staining with Coomassie Brilliant Blue.
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bioRxiv - Immunology 2024Quote: ... Membranes were then blocked with 5% non-fat milk for one hour at room temperature (Bio-Rad, Hercules, CA, USA). After blocking ...
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bioRxiv - Cell Biology 2024Quote: ... and ∼140-150nt DNA fragments were then size-selected by gel electrophoresis with 5% TBE gels (Bio-Rad, Cat #4565015) following NEB library prep kit protocol ...
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bioRxiv - Neuroscience 2024Quote: ... boiled for 5 min at 95 °C and then loaded on 4–15% Mini-PROTEAN TGX Precast Gels (Bio-Rad). Gels were transferred onto polyvinylidene fluoride membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were washed four times with PBS-T for 5 minutes and chemiluminescent signals were detected using Clarity Western ECL Substrate (BioRad,), and visualised using the ImageQuant® LAS-4000 imager (GE Healthcare ...
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bioRxiv - Pathology 2024Quote: ... Total reaction volume was 10 µl and contained 5 µl of iTaq Universal SYBR green one-step kit (Bio-Rad), 300 nM of forward and reverse primers (Table S5 ...
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bioRxiv - Cell Biology 2024Quote: ... before heating at 95°C for 5 minutes and finally loaded onto 4-20% polyacrylamide gel (Bio-Rad, Cat # 4561094). Samples were then transferred to a PVDF membrane using the Trans-Blot® Turbo™ Transfer System (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were resuspended in FACS buffer and acquired with a 5-laser Bio-Rad ZE5 flow cytometer (Bio-Rad). FACS data were analyzed using FlowJo version 10.9 software (BD Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples (25 µl total volume) were heated 5 minutes at 95 °C and loaded onto an AnyKD precast MiniProtean TGX Stain Free gel (Biorad) or an 15% SDS-PAGE gel ...
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bioRxiv - Neuroscience 2024Quote: ... A preliminary qPCR was then performed with a fraction of the reaction product to determine the optimal number of amplification cycles needed for a second round of amplification without saturation: to this end 5 µL of iQ SYBR Green Supermix (Biorad) were added to 5 µL preamplified DNA and used for qPCR (98°C 30 s denaturation) ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then run on 10 % SDS-PAGE gel for 5 cm long in separation gel and stained using Bio-Safe Coomassie Stain (BioRad).
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bioRxiv - Neuroscience 2024Quote: ... Samples were then denatured for 5 min at 95°C with 50 μl of 2X sample buffer (BioRad, cat#1610737EDU) with β- mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... and then buffer exchanged into 150 mM ammonium acetate pH 7.5 by two consecutive passes over centrifugal gel filtration columns (Micro Bio-Spin P-6, 6-kDa cut off; BioRad). Protein concentration was verified by A280 ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were run for 5 min at 130 V followed by 55min min at 180 V (PowerPac Basic Power Supply (Biorad)) ...
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bioRxiv - Cancer Biology 2024Quote: ... The calibration curve for each cytokine was analysis with 5-parametric logistic curve regression using Bio-Plex manager software (ver. 5.0, Bio-Rad). ELISAs were performed with quantification of absorbance using a spectrophotometer (BioTek) ...
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bioRxiv - Cell Biology 2024Quote: ... and 5-10 μg aliquots were fractionated by SDS-PAGE and then transferred to nitrocellulose 0.45 μm membrane (Bio-Rad). Membranes were blocked for 1 h at room temperature in 5% BSA in 1x Tris-buffered saline + 0.1% Tween 20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... and 1’,3’-bis[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho]-glycerol (16:0-18:1 Cardiolipin) were spotted using a Hamilton syringe onto a nitrocellulose membrane (Biorad trans-blot turbo RTA Midi 0.2 µm) to yield 10 ...
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bioRxiv - Microbiology 2021Quote: ... 10 or 1 µl of total lysate and 10 or 1 µg of OMVs were separated on Criterion™ TGX Stain-Free™ any kD™ gel (Bio-Rad, Hercules, California, USA) for Coomassie staining or Western Blot ...
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bioRxiv - Immunology 2020Quote: ... a total of 6 × 107 cells was incubated in staining buffer with mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:50) at 4°C for 20 min ...