Labshake search
Citations for Bio-Rad :
4801 - 4850 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Lysates were then denatured in 2X Laemmli at 95°C for 5 minutes and run in Mini-PROTEAN Precast Gels (BioRad) and transferred onto membranes using Trans-Blot Turbo ...
-
bioRxiv - Genetics 2020Quote: ... The membrane was washes in TBS-T three times and incubated 5 min with Clarity Western ECL Substrate (#1705061, Biorad) in order to detect proteins immunoblotted signals at Alliance LD4 (UVItec Cambridge ...
-
bioRxiv - Biochemistry 2020Quote: ... The samples were heated at 95°C for 5 min and separated on a 12% Bis-Tris SDS-PAGE gel (Biorad).
-
bioRxiv - Synthetic Biology 2020Quote: ... 5 or 10 μL of the supernatant were resolved on an Any kD TGX Stain-Free protein gel (4568126, BioRad) alongside a Chameleon Duo Pre-Stained Protein Ladder (928-60000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... After centrifugation (50 x g for 5 min) the Ni-NTA agarose beads were transferred to an empty Bio-Spin Chromatography column (Biorad). The column was extensively washed with 20 CV (column volume ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were diluted into 5 ml 1X PBS and sorted and analysed with a S3e(tm) Cell Sorter and ProSort(tm) Software (BioRad). Fluorescence intensities of cells were measured using 488 nm and 561 nm lasers ...
-
bioRxiv - Biochemistry 2020Quote: Aliquots (250 μg total protein) of hippocampal extracts of WAR animals subjected to freeze/thaw were treated with 5 μL of dithiothreitol (Biorad) 50 μg/μL ...
-
bioRxiv - Neuroscience 2020Quote: ... gels (4.5% acrylamide stacking gel and 10% acrylamide resolving gel) and were then transferred to 0.45 µm nitrocellulose membranes (Bio-Rad) using a semi-dry transfer system (Trans-Blot Turbo ...
-
bioRxiv - Genomics 2021Quote: ... Libraries generated by the same protocol were pooled and separated on 5% TBE-PAGE on Mini-PROTEAN tetra cell (BioRad) (Suppl.5 ...
-
bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: qPCR reactions were carried out in 25 µl total volume containing 5 ng of cDNA sample and 0.3 µM designed primers and SYBR Green Master Mix (Bio-Rad). Amplification was performed by Step One Plus (Applied Bio System ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
-
bioRxiv - Physiology 2021Quote: ... membrane-enriched fraction: 5 μg per lane) were loaded onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA) – alternating between control and high CO2 treatments to avoid possible gel lane effects ...
-
bioRxiv - Immunology 2020Quote: ... and membrane blocking was performed overnight at 4°C in Tris-buffered saline (TBS) containing 0.2% Tween 20 (V/V) (TBST) and 5% (W/V) Blotting-Grade Blocker (Bio-Rad). Following overnight blocking ...
-
bioRxiv - Molecular Biology 2020Quote: ... proteins in loading buffer were boiled for 5 min before loading on to 12-15% SDS-PAGE gels using the Mini-Protean III gel system (BioRad). A protein transfer apparatus (BioRad ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were stripped using Restore™ Western Blot Stripping Buffer (Cat. number: 21059) following the manufacturer’s protocol and blocked with 5% Blocking-Grade Blocker solution (Bio-Rad) for 2 h at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... Mean fluorescence intensity was measured to calculate final concentration in pg/mL using Bioplex200 and Bioplex Manager 5 software (Biorad).
-
bioRxiv - Plant Biology 2023Quote: ... membranes were washed at least three times for 15 minutes in 1x TBST solution and incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
-
bioRxiv - Plant Biology 2023Quote: ... Membranes were washed three times for 15 minutes in 1x TBST and immunoblots were incubated for 5 minutes at room temperature with either Clarity Western ECL (BioRad) or Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... PFGE was undertaken by running samples on a 1.0% agarose gel in 0.5 X TBE solution at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, BioRad). 6 V/cm were used with 15 h switch time of 60 s followed by 15 h 120 s at 120° ...
-
bioRxiv - Microbiology 2022Quote: ... pBAC-BoHV-4-A-revORF45HA and pBAC-BoHV-4-A-ΔORF45KanaGalK DNAs (5 μg) were electroporated in 600 μl DMEM high without serum (Biorad, Gene Pulser XCell ...
-
bioRxiv - Microbiology 2023Quote: ... Protein preps were boiled for 5 minutes and then separated by SDS-PAGE on a 10-20% Tris-Gycine gel (BioRad). Proteins were electro-transferred from gels to nitrocellulose membranes ...
-
bioRxiv - Cancer Biology 2023Quote: ... membranes were washed in 4 x 5 minutes in 0.2% TBS tween before incubating in Clarity™ Western ECL Substrate (BioRad) for 5 minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... 7% acetic acid for 30 minutes at room temperature followed by two 5-minute washes in Mill-Q water for imaging on the ChemiDoc (BioRad). Alternatively ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by 40 cycles of 95 °C for 5 sec and 60 °C for 30 sec using the CFX 96 real-time systems (Biorad). The 2−ΔΔCt method was used to analyze the data (Schmittgen and Livak ...
-
bioRxiv - Microbiology 2023Quote: ... The membrane was then washed three times with TBS-T for 5 min each before developing using Clarity MaxTM Western ECL Substrate (1705062; BioRad) and imaged using a BioRad ChemiDoc XRS+.
-
bioRxiv - Cell Biology 2023Quote: ... The secondary antibody was washed away 5× with TBS-T and developed with an AP Conjugate Substrate Kit (Bio-Rad). Spots were quantified using a Nikon ELISPOT system and NIS-Elements AR software (v ...
-
bioRxiv - Cell Biology 2023Quote: ... The oligonucleotide substrate products were visualized using the ChemiDoc MP Imaging System to detect Cy 5 fluorescence (Bio-Rad, 1708280).
-
bioRxiv - Microbiology 2023Quote: ... Blot sections were washed 5 × 5minutes in TBST and signal was detected using Clarity Western ECL Substrate (BioRad 170 5060). Images were captured on a BioRad Chemi Doc XRS+ imager.
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein (5 µg) were loaded into each lane and separated on 4– 20% polyacrylamide Tris glycine SDS gels (BioRad), then transferred to 0.45 µm PVDF membranes (Millipore Sigma) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... the hybrid RNA/DNA origami was run on 1.5% agarose gel in TAE buffer (75 mL were poured into a 15 cm x 10 cm gel tray, Biorad) and analysed by in-gel imaging using the specific dye DFHBI-1T as described above with few modifications due to the different gel composition (agarose instead of polyacrylamide ...
-
bioRxiv - Neuroscience 2022Quote: ... R primer: 5′-GTACCACCATGTACCCAGGC-3’) cDNA were amplified using the iScript RT-PCR iQ SYBR Green Supermix (BIORAD, Cat.:# 170882) in a qPCR detection system (LightCycler LC480 ...
-
bioRxiv - Microbiology 2022Quote: ... Cell equivalents of ~1.5×106 cells per lane were separated by 10% SDS-PAGE along with the Precision Plus Protein Kaleidoscope standard (BioRad). The fractionated proteins were transferred to a PVDF membrane ...
-
bioRxiv - Neuroscience 2022Quote: ... After resuspension in TeSR/E8 medium supplemented with 5 μM ROCK inhibitor cells were counted with a TC20 automated cell counter (Biorad) and resuspended to get a final concentration of 2 x 10^5 cells/ml ...
-
bioRxiv - Neuroscience 2024Quote: ... Stain-free protein gels were activated with UV light for 5 min and then transferred to PVDF membranes (Bio-Rad) using a turbo transfer system (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.01% bromophenol blue) and denaturated 5 min at 95°C before protein separation by SDS–PAGE on 4-15% precast gels (Biorad).
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was blocked with B-TBST (TBS buffer with 0.05% Tween-20 and 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membranes were then blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membranes were then blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature ...
-
Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... Expression studies were further carried out using 100 ng of cDNA template and 0.5 µM (each) of specific primers in a Bio Rad CFX96 real time PCR with SyBr Green iTaq Mix (Biorad). Data generated was further analysed using 2-ΔΔCT method ...
-
bioRxiv - Cell Biology 2023Quote: ... and boiled for 5 min at 95 C and loaded onto an or 4-20% TGX stain-free polyacrylamide gels (Biorad), followed by standard western blotting procedures ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples were eluted by heating at 100°C for 5 min in Laemmli sample loading buffer (Bio-Rad, USA), and resolved on an SDS/PAGE ...
-
bioRxiv - Cell Biology 2023Quote: ... 150ul of the suspension was filtered through a 40μM cell strainer at 40G for 5 minutes to remove clumped cells and subsequently used for analysis on the ZE5 flow cytometer (Biorad). Analysis of gene expression was analyzed using the Easyflow MATLAB script (https://antebilab.github.io/easyflow/) ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 50°C and 5 minutes at 85°C in T100 Thermal Cycler (1861096, Bio-Rad). Real-time qPCR reactions were performed using SsoAdvanced Universal SYBR® Green Supermix (1725274 ...
-
bioRxiv - Biochemistry 2023Quote: ... Gels were run for 5 min at 130 V followed by 55min min at 180 V (PowerPac Basic Power Supply (Biorad)) ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples adjusted to contain equal amounts of protein (10 μg or 12 μg) were next denatured (5 min at 95°C) in loading buffer (#1610747, BioRad) and run on 10% TGX (#4561034 ...
-
bioRxiv - Microbiology 2023Quote: ... 0.5 μl of this cell suspension was then placed on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) TGY2X pad prepared on a cover glass using a gene frame (Thermo Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 95 °C for 5 minutes and separated on precast 4-20% Criterion TGX gels (Bio-Rad). The competition of IA-rhodamine was analyzed by in-gel fluorescent signal using a ChemiDoc MP (Bio-Rad) ...