Labshake search
Citations for Bio-Rad :
4751 - 4800 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... samples were incubated for 5 minutes (tissue ≤ 40μm) or 10 minutes (tissue ≥ 100μm) in polyacrylamide (PA) solution [4% acrylamide/bis acrylamide (BioRad., 1610154), 60mM Tris HCl pH 8 (Corning ...
-
bioRxiv - Molecular Biology 2024Quote: ... whereafter 5 μl was used for a 25 μl qPCR reaction using 2x SYBR Green Master Mix (Bio-Rad) in a CFX96 Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... which were boiled at 95°C for 5 minutes before loading in pre-cast PAA gels (Bio-RAD, 4561084) and run at 100V for approx ...
-
bioRxiv - Microbiology 2024Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... we incubated the samples 5 min at 65°C in a C1000 thermal cycler (Bio-Rad, Hercules, CA, USA). We added a synthesis mixture of 10 mM deoxynucleoside triphosphates ...
-
bioRxiv - Molecular Biology 2024Quote: ... The lysate was clarified and incubated at 55°C for 5 minutes with Laemmli sample buffer (1610747, Bio-Rad). Samples were run on a 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gel (4568086 ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants were then collected by centrifugation at 20,000 × g and mixed with 5 µg/mL Ethidium bromide (EtBr, BioRad). Fluorescence was measured using a CLARIOstar plate reader (BMG ...
-
bioRxiv - Cancer Biology 2024Quote: ... 20lilµg of total protein from each sample were subjected to 7.5% or 10% SDS polyacrylamide gel electrophoresis (PAGE) and electrophoretic transfer onto 0.2lilµm polyvinylidene difluoride (PVDF) membrane (Bio-Rad) at 30lilV ...
-
bioRxiv - Bioengineering 2024Quote: ... for an additional 5 minutes before imaging using the rapid-auto protocol on a ChemiDoc Imaging system (Bio-Rad). Each blot was verified with two additional replicates.
-
bioRxiv - Developmental Biology 2024Quote: ... and qPCR performed with 5 ng of cDNA and the Sso Advanced Universal SYBR Green Supermix (Bio-Rad, 1725270) in a Bio-Rad CFX-384 Real Time Thermal Cycler ...
-
bioRxiv - Cell Biology 2024Quote: ... for two rounds of electroporation at 850 V and 25 μF with an interval of 5 s (Bio-Rad Gene Pulser Xcell Electroporation Systems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated in horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature and finally developed using Bio-Rad ChemiDoc Imaging System (Bio-Rad, Hercules, CA, USA, cat# 12003154) using ECL substrates (ThermoFisher ...
-
bioRxiv - Microbiology 2023Quote: ... TCA precipitated protein mixtures were dissolved in 30 µL 1 x Laemmli sample buffer and loaded on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories, Germany). After short 3 cm SDS PAGE separation ...
-
bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
-
bioRxiv - Cell Biology 2024Quote: ... Secondary horseradish peroxidase-coupled antibodies (1:3000, Pierce, α-mouse #1858413, α-rabbit #1858415) were applied prior to ECL-based chemiluminescence (Biorad #1705061 and ThermoFisher #34094) detected using a Fusion Fx instrument (Vilber Lourmat) ...
-
bioRxiv - Microbiology 2024Quote: ... alongside a DNA Marker 1, 100-1000 bp marker ladder (A&A Biotechnology, Gdynia, Poland) using a PowerPacTM Basic (Bio-Rad Laboratories Inc., Hercules, CA, USA) at 95 V and 400 mA ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were then transferred using a semi-wet transfer approach into Nitrocellulose blotting membranes (Carl Roth #200H.1) using the Trans-Blot® Turbo™ Transfer Starter System (Bio-Rad Laboratories, CA, USA). Membranes were then stained with Ponceau S (Sigma #P7170-1L ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5’KI and 3’KI as detailed (Fig 2A and D) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad). Briefly ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
-
bioRxiv - Developmental Biology 2021Quote: ... by Bio-Rad tans-blot turbo transfer system at mixed weight transfer setting and incubated in 5% blotting grade blocker (BioRad Catalog: 1706404) solution for 1 hour ...
-
bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was obtained by incubating the samples (whole embryos or adult caudal fin fragments) in TE buffer supplemented with 5% Chelex-100 (BioRad) and 10 μg/ml Proteinase K (Roche ...
-
bioRxiv - Biochemistry 2022Quote: ... Tween 20 (TBS-T) in 5% (w/v) milk (pH 7.4) followed by incubation with an anti 6xhistidine antibody (Bio-Rad) overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... The dried pellets enriched for histones were dissolved in sample buffer (8 M urea, 5% β-mercaptoethanol and 10 mM Tris-HCl (pH 7.0)) and added sample loading buffer (Biorad XT sample buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... The blots were washed four times with TBS-T for 5 min each prior to developing with Clarity Western ECL Blotting Substrates (BioRad) and imaging on a UVP BioSpectrum Imaging System ...
-
bioRxiv - Developmental Biology 2022Quote: ... the gel was incubated in 1x TBE for 10 min and after was stained with Green Safe reagent dye (5 µL /100 mL of TBE 1x) for 30 min and visualized on ChemiDoc XRS+ system (BioRad).
-
bioRxiv - Biochemistry 2020Quote: ... proteins were dialyzed in HEPES sodium salt-HCl (75 mM, pH 7.0) with Chelex-100 resin (5 g/L, Bio-Rad) to remove any divalent metal contaminants ...
-
bioRxiv - Plant Biology 2021Quote: ... The blot was washed with TBST (TBS with 0.1% tween 20) and was blocked with either 5% blotting grade blocker (Bio-Rad) or 4% BSA (Sigma ...
-
Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... The 20 μL reaction mix contained 5 μL sample or standard DNA together with 10 μL 2x SsoAdvanced Universal Probes Supermix (Biorad), 1 μL of each primer (5 μM stock solution) ...
-
bioRxiv - Biochemistry 2021Quote: ... and the proteins containing 5× SYPRO™ Orange were loaded onto Hard-Shell® 96-well PCR Plates (Bio-Rad) for measurement ...
-
bioRxiv - Biochemistry 2021Quote: ... The membrane was first blocked with B-TBST (TBS buffer with 0.05% Tween-20 and supplemented with 5% blotting grade non-fat dry milk; Bio-Rad) for 1 hr at room temperature and subsequently blotted with the primary antibodies in B-TBST overnight at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... The resin was resuspended in 5 mL of extraction buffer and transferred to a gravity flow column (Biorad, Hercules, CA). After loading ...
-
bioRxiv - Biophysics 2021Quote: ... 5 µL of purified (mutant) protein sample was mixed with 1.67 µL of 4X Laemmli loading buffer (Bio-Rad 1610747), then 5 µL was loaded onto an SDS-PAGE gel (Either 12% isocratic (Bio-Rad 4561046 ...
-
bioRxiv - Cell Biology 2021Quote: ... 25 µg of protein lysate was heat-denatured at 95°C for 5 minutes in Laemmli sample buffer (Bio-Rad), and then separated using a 4 to 12% Bis-Tris polyacrylamide gel (Invitrogen ...
-
bioRxiv - Microbiology 2021Quote: Isothermal assembly reaction buffer (5×) (500 mM Tris-HCl [pH 7.5], 50 mM MgCl2, 50 mM dithiothreitol [DTT] [Bio-Rad] ...
-
bioRxiv - Molecular Biology 2020Quote: ... Mean fluorescence intensity was measured to calculate final concentration in pg/mL using Bioplex200 and Bioplex Manager 5 software (Biorad).
-
bioRxiv - Cancer Biology 2020Quote: ... Target sequences in cDNA library were amplified in 10 μl qPCR reaction (5 μl SYBR Green supermix (Bio-Rad #1725121), 0.675 μl 2.5 μM primer mix and 0.45 μl diluted cDNA ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to PVDF membranes and blocked in TBS with 0.1% Tween-20 (TBST) and 5% Blotting Grade Buffer (BGB, Bio-Rad). The anti-RBM20 rabbit polyclonal primary antibody (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... samples were loaded onto Mini-PROTEAN TGX Pre-cast Stain-Free gels (5-15%, Bio-Rad Laboratories, Hercules, CA, USA) and total protein was visualised post-transfer to PVDF membranes on ChemiDoc Touch Imaging System ...
-
bioRxiv - Biophysics 2021Quote: ... 10 mM borate pH 10 was prepared at 5 μM and loaded onto a 4-20% Mini-Protean TGX pre-cast gel (BioRad). 10 μL of each folding sample was subsequently loaded onto the same gel ...
-
bioRxiv - Biochemistry 2022Quote: ... by loading samples onto a 0.5X TBE 5% polyacrylamide gel prepared for a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad) using ProtoGel acrylamide (National Diagnostics) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The beads were washed 5 times and denatured with 4x Laemmli sample buffer (Cat: 161-0747, BioRad, Hercules, CA, USA) at 100 C for 5 min ...
-
bioRxiv - Microbiology 2022Quote: ... 5 ng of each sample was loaded into a well of a 10% Mini-PROTEAN TGX Precast Gel (Bio-Rad). Gels were transferred to PVDF membranes (Merck KGaA ...
-
bioRxiv - Genomics 2022Quote: Slot blot was performed using 1.5 ng of gDNA that was denatured in 400 mM NaOH/10 mM EDTA and blotted onto nitrocellulose membrane (BioRad) in duplicate for dsDNA and 5mC DNA using a slot blot apparatus (BioRad) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted by heating the beads at 95°C for 5 min in Laemmli Sample Buffer (1610747, Bio-Rad) with 50 mM DTT followed by magnetic separation ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed with IP lysis buffer three time and boiled in 25 ul of 2X SDS-loading buffer for 5 minutes and loaded into 4-15% polyacrylamide gels (BioRad).
-
bioRxiv - Microbiology 2021Quote: ... The protein from the 5% of beads was eluted by boiling the beads in Laemmli sample buffer supplemented with BME (BioRad) and 2 mM biotin at 90°C for 10 min ...
-
bioRxiv - Neuroscience 2022Quote: ... heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl; BioRad) and transferred onto Immun-Blot PVDF 0.2 µm (BioRad ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 ng of cDNA was analyzed with isoform-specific droplet digital PCR assays and ddPCR Supermix for probes (Bio-Rad). Primers and probes (Integrated DNA Technologies ...
-
bioRxiv - Biochemistry 2022Quote: ... The PCR reaction was carried out in 10 μl volume with 5 μl of 2X iTaq Universal Sybergreen Supermix (BioRad), 500 nM of forward and reverse primers (for promoter ...