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Citations for Bio-Rad :
4701 - 4750 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed in a solution of TBS+0.01 % Tween-20 (TBS-T) and incubated in 5 % non-fat milk (Biorad) blocking solution for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... 5 to 50 µg of DNA was electroporated (375 V, 1,000 µF, 750 Ω; GenePulser Xcell; BioRad, Hercules, CA) into trophozoites ...
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bioRxiv - Cell Biology 2020Quote: ... Standard qPCR reactions with 5 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed on 5 ng/μL cDNA using iTaq Universal SYBR Green Supermix (Bio-rad, 64361172) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
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bioRxiv - Microbiology 2020Quote: ... purified on Ni-NTA resin (G-Biosciences, #786-940 in 5 ml Bio-Scale™ Mini Cartridges, BioRad #7324661), then dialyzed ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked with 5% (w/v) non-fat milk tris-buffered saline (TBS) with 0.1% Tween-20 (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... pH 8.3) running buffer by loading 5 μg sample onto 4-20% Mini-PROTEAN® TGXTM gels (Bio-Rad) with iBrightTM Prestained Protein Ladder (#LC5615 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Genomic DNA was obtained from single adults via incubation in 100µL of 5% w/v Chelex beads (BioRad Inc.) at 55°C ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL blocking buffer supplemented with 5 μL of horseradish peroxidase (HRP) conjugated rabbit anti-mouse secondary antibody (BioRad) was added and incubated for 2 hours at room temperature with agitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 15μg protein in 5x Laemmli buffer with 5% b-mercaptoethanol was loaded on Mini-PROTEAN TGX Precast Gels (BioRad). Gel lanes were cut into three sections for peptide extraction ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 800 µl of cell suspension mixed with 10 µg of pDM304_Red-FL2 in a 4-mm-width cuvette was subjected to electroporation (two 5 sec separated pulses with 1.0 kV and a 1.0 msec time constant) using a MicroPulser (Bio-Rad). These cells were plated on 4–6 pieces of 90-mm plastic dishes with HL5 medium and incubated at 22°C for 18 hours under the non-selective conditions and then cultured in the presence of 10 µg/ml G418 (Wako) ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome quality was analyzed by running the nucleosome on native PAGE on a 5% TBE gel in 0.5X TBE buffer (BioRad) that was run for 60 min at 150 volts ...
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bioRxiv - Biochemistry 2021Quote: ... was added to a final concentration of 1X to the quenched reaction and samples were loaded onto a 5% TBE gel and run for 60 min at 150 volts in 0.5X TBE Buffer (BioRad). Gels were stained with ethidium bromide and imaged on a BioRad ChemiDoc ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.5] with 0.1% (v/v) Tween 20) containing 5% Blotting-Grade Blocker (Bio-Rad Laboratories, cat. #170-6404) for 30 mins ...
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bioRxiv - Microbiology 2023Quote: ... both proteins were supplemented with 5 mM MgCl2 and protein concentration was determined using a colorimetric assay (Bio-Rad). Proteins were then mixed at a 2.5:1 mass ratio of KtrC to N-terminal Strep-tagged KtrA (11 mg of KtrC and 4.5 mg of N-terminal Strep-tagged KtrA in 10 mL total volume of 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf lysates were cleared by centrifugation at 10,000×g for 5 min and total protein normalized via Bradford Assay (Biorad). Extracts were prepared for SDS-PAGE with a 5x loading buffer containing 10% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with 5% non-fat milk in PBS-T (0.5% Tween-20 [1706531, Bio-Rad] in PBS) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µL of the supernatant was loaded into a 4−20 % Mini-PROTEAN-TGX gel (BioRad, Hercules, CA, USA), run at 165 V for 50 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Each reaction consisted of 5 μl of extracted DNA and 15 μL of iTaq™ Universal Probes Supermix (BioRad), containing 0.4 mM of each primer ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed twice with water and resuspended in 20 µL Laemmli sample buffer (Bio-Rad, 2X with 5% β-mercaptoethanol). After boiling for 5 min at 100°C ...
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bioRxiv - Biochemistry 2024Quote: ... The enzyme was purified using a 5-mL Ni-charged EconoFit Nuvia IMAC Column (Bio-Rad, Hercules, CA, USA) and a linear gradient of 10 - 250 mM imidazole in binding buffer (100 mL in total at 2.5 mL/min flow rate) ...
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bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
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bioRxiv - Biophysics 2024Quote: ... Blots were washed 3 times with TBST for 5 min and visualized using a ChemiDoc MP Imaging System (Biorad).
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bioRxiv - Neuroscience 2024Quote: ... After 1h-incubation with HRP-conjugated secondary antibodies diluted in 5% milk in TBST (anti-mouse HRP (BioRad, 1706516) 1:10000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: Quantitative PCR (qPCR) was performed in 10 µL reactions containing 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was loaded onto a 5 mL Bio-Scale Mini Nuvia IMAC Ni-charged Cartridges (Bio-Rad) which was pre-equilibrated with 5 column volumes (CV ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were heated to 95°C with 2X sample buffer for 5 mins before loading the samples directly to a 0.2μm nitrocellulose (BioRad, #1704159) membrane ...
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bioRxiv - Microbiology 2024Quote: ... and then lysed by a 5-minute incubation with with 50 uL iScript RT-qPCR Sample Preparation Reagent (BioRad) (64) ...
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bioRxiv - Immunology 2022Quote: ... Internal loading control was performed by stain free visualization after 5 min activation on a ChemiDoc imaging system (BioRad). Protein transfer was done either on nitrocellulose membrane or polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2022Quote: ... 5% β-mercaptoethanol) to be run on SDS-PAGE gels using the Mini Protean system (Bio-Rad, no. 1658004). Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... the membrane was blocked using 5% non-fat milk in 0.1% TBS-T (1X Tris-Buffered Saline, 0.1% Tween 20, Bio-Rad) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and then boiled for 5 minutes before being loaded into either a precast 10% polyacrylamide gel (4561034, Bio-Rad) or manually made gel for electrophoresis with Tris-glycine-SDS running buffer (24.8 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... samples were loaded onto Mini-PROTEAN TGX Pre-cast Stain-Free gels (5-15%, BioRad Laboratories, Hercules, CA, USA) and total protein was visualised post-transfer to PVDF membranes on ChemiDoc Touch Imaging System ...
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bioRxiv - Cell Biology 2023Quote: ... To visualize the protein bands, membranes were incubated (5 min, RT) in Clarity™ western ECL substrate (Bio-Rad) and imaged using the ChemiDoc™ MP imaging system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... The transferred membrane was briefly washed with TBST (0.1% Tween-20 in TBS) and blocked with Blocking Buffer (5% Blotting-Grade Blocker [1706404, BioRad] or 5% BSA [A9647-50G ...
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bioRxiv - Immunology 2023Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primer ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 μg/sample was loaded onto 4-15% gradient polyacrylamide gels (Mini-PROTEAN TGX Gels, Bio-Rad, 4561083), transferred (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... and 20 μl were mixed with 5 μl 5x Lämmli buffer for loading on a 10% SDS-PAGE gel (BioRad). After blotting was done on nitrocellulose using the semi-dry system (BioRad) ...
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bioRxiv - Plant Biology 2023Quote: ... at 1:10,000 dilution in 5% milk PBST and ECL prime chemiluminescent reagent (Amersham: RPN2236) on a ChemiDoc gel imager (Biorad).
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was clarified and incubated at 55°C for 5 minutes with Laemmli sample buffer (1610747, Bio-Rad). Samples were run on a 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gel (4568086 ...