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Citations for Bio-Rad :
5051 - 5100 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative PCR (qPCR) was performed in a 10 µL reaction that contained 5 µL SsoAdvanced™ Universal SYBR Green Supermix (BioRad), 1 µL H2O ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein was denatured at 95°C for 5 min in 1x Laemmli buffer containing 5% β-mercaptoethanol and separated in 4-15% mini-PROTEAN TGX Precast Protein Gels (Biorad) by electrophoresis and blotted onto Nitrocellulose membrane (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 3 μl ten-fold diluted cDNA preparation was mixed in a final volume of 10 μl containing 5 μl iQ SYBR Green Supermix (Bio-Rad) and 4 pmol of each primer (Fw ...
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bioRxiv - Cell Biology 2023Quote: ... 10µg and 5 µg /sample) were resolved by SDS-PAGE on Mini-PROTEAN TGX Stain-Free Gels (Cat. # 4568086; Bio-Rad) and were run alongside a Page Ruler prestained protein ladder (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 2 × 106 of MEL cells were suspended in 100ul of High-Performance Electroporation Solution (BTXpress, 45-0801) with 5-10 µg of plasmid DNA and electroporated with the Gene Pulser Xcell System (Bio-Rad) using 2 pulses at 200 V for 5 ms ...
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bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
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bioRxiv - Molecular Biology 2023Quote: ... About 15 μg of protein samples from lysates were boiled at 95°C for 5 min in Laemmli sample buffer (Bio-Rad) and resolved in 4-15% gradient SDS-PAGE gels (Mini-Protean TGX ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Synthetic Biology 2023Quote: ... The membrane was washed 3 times with TBST for 5 min each and then the membrane was incubated with Clarity Western ECL Substrate (Bio-Rad) for 2 min before exposure to film and development in a darkroom.
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bioRxiv - Cell Biology 2023Quote: ... Proteins samples were denatured in SDS-sample buffer for 5 min at 95°C and loaded onto Mini-Protean TGXTM gels (BioRad Laboratories) in equal amounts and subjected to electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were heated to 95 °C for 5 min and separated on 15% TBE with urea polyacrilamide gel (BioRad Cat# 3450093) at 200 V for 60 min ...
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bioRxiv - Biophysics 2024Quote: ... Staining was performed with 2X SYBR Gold in 1X TBE buffer for 5 minutes and the gel was imaged using the ChemiDOC MP imaging station (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Proteins samples were denatured in SDS-sample buffer for 5 min at 95°C and loaded onto Mini-Protean TGXTM gels (BioRad Laboratories) in equal amounts and subjected to electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... Electroporation was carried out with 3-5 μL of the linear PCR product (metH homology extension-flanked kan cassette) at 2.5 kV (Bio-Rad MicroPulser), and cells were immediately recovered with 1 mL SOC and cultured at 37°C for one hour ...
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bioRxiv - Immunology 2024Quote: ... were washed with 500 μl ice-cold acetone to remove TCA at 16,000g for 5 min at 4 °C and resuspended in 100 μl 1x XT sample buffer (Bio-Rad #1610791), boiled at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated for 5 minutes at 95 °C followed by 60 minutes at 60 °C using Thermocycler (Bio-rad, U.S). The samples were stored in −20 °C until use ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Treated whole cell extracts (5 µg per lane) were resolved on a 12% TGX Stain-free polyacrylamide gel (Bio-Rad, #1610185) by applying 180V until loading dye ran off the gel ...
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bioRxiv - Biophysics 2024Quote: ... 5 µL of sample was incubated with 195 µL of Bradford reagent (Quick Start™ Bradford 1x Dye Reagent #5000205, BioRad). Absorbance was measured at 550 nm after a 5-10 minutes incubation protected from the light ...
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bioRxiv - Cancer Biology 2024Quote: ... This was followed by a final extension at 72°C for 5 minutes and melt curve analysis on the CFX96 Real-Time System (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... boiled at 950C for 5 min and loaded into each lane of a 4–15% Mini-PROTEAN TGX Stain-Free precast gel (Bio-Rad). Colour Prestained Protein ladder was loaded in some wells in order to determine the molecular mass of proteins in the experimental samples ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then washed 4 times with PBST and then incubated in the dark for 5 min with Clarity Western ECL substrate (1705060, Bio-Rad), and imaged using the Amersham Imager 600 (GE Industries ...
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bioRxiv - Plant Biology 2024Quote: ... heat-denatured at 95°C for 5 min and separated by SDS-PAGE on home-made gels or 4-15% precast polyacrylamide gel (Bio-rad).
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then washed again for 3 x 5 min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...
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bioRxiv - Molecular Biology 2024Quote: ... using a 5 μL PCR reaction volume with SYBR Green supermix (SsoAdvanced™ Universal SYBR® Green Supermix, Bio-Rad, USA). All samples were run in duplicate simultaneously with template free controls ...
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bioRxiv - Neuroscience 2024Quote: ... The appropriate secondary fluorescent antibodies were applied for 1 h at room temperature and then washed off three times for 5 min each before the signals were visualized by the ChemiDoc Imaging system (BIO-RAD). Signal intensities were then measured by ImageJ (https://imagej.nih.gov/ij/index.html).
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bioRxiv - Neuroscience 2024Quote: ... heated at 95 °C for 5 min and loaded on stain-free 4–15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis gels (Bio-Rad). Separated proteins were transferred onto a Polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... 10 µg of total proteins were denatured at 95°C for 5 min in 100 mM dithiothreitol (DTT) and 1x Laemmli buffer (Bio-Rad). Proteins mixtures were separated using a Mini-PROTEAN Tetra Vertical Eletrophoresis Cell for Mini Precast Gels apparatus (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... The maleamide reaction was quenched with 5 mM DTT and unreacted Halo Ligand and Quencher were removed using two Bio-Spin 6 (Tris) columns (Bio-rad).
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bioRxiv - Neuroscience 2024Quote: ... sample protein (5-8 µg) and molecular weight ladders were loaded onto a 4–15% Criterion TGX precast gels (Bio-Rad) for gel electrophoresis separation ...
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bioRxiv - Plant Biology 2024Quote: Protein samples were denatured for 5 min at 95°C and separated in TGX Stain-Free FastCast 10% Acrylamide gel (Biorad #1610183) using running buffer (25 mM Tris base pH 8.3 ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Wells were washed five times with PBS+0.05% Tween 20 and blocked with PBS+5% dry milk solution (Bio-Rad, 1706404) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was then heated to 95 °C for 5 min and loaded into pre-cast polyacrylamide gels (Mini-PROTEAN TGX, Bio-Rad) for electrophoresis in TGS buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1/5 and quantified by RT-qPCR in 10 μL final reactions using 2X iTaq Universal Sybr Green Supermix (#1725125, Bio-Rad) in CFX96 Touch Real-Time PCR machines (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 vol of DTT 1M was added and the mixture heated at 95°C for 5 min before loading onto Any kD Mini-PROTEAN TGX Stain-Free protein gel (4568124, Bio-Rad) and migration in 1x Tris-Glycine-SDS buffer at 200V ...
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bioRxiv - Biochemistry 2024Quote: ... These samples were spun down for 5 min at 16000× g before loading 10 µL into 10 % Mini-PROTEAN TGX precast gels (Bio-Rad). The gel was run on gel electrophoresis at 150 V for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was washed 3 times with TBST for 5 minutes and was developed with Clarity Max Western ECL Substrate (Bio-Rad) for 1 minute before imaging ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were boiled at 95°C for 5 min and proteins were separated on 4–15% Mini-Protean TGX precast protein gel (Bio-Rad) and transferred onto hydrophobic polyvinylidene fluoride (0.45 μm PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were first incubated with Amido Black to verify uniform loading and incubated with blocking buffer (5% Blotting-grade Blocker (Bio-Rad) in Tris-Buffered Saline ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μg protein/sample was denatured at 95 °C for 5 min in Laemmli buffer with 2.5% ß-mercaptoethanol and separated with a 4–20% Mini-PROTEAN (Bio-Rad) polyacrylamide gel in 25 mM Tris ...
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bioRxiv - Synthetic Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed on 5 ng of cDNA with iTaq Universal SYBR Green Supermix (BIO-RAD) in a CFX Connect Real Time PCR system (BIO-RAD) ...
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bioRxiv - Cancer Biology 2024Quote: ... heated at 95 °C for 5 min followed by loading on a 10% precast polyacrylamide gel (Bio-Rad, Hercules, California, USA). The separated bands were visualized using a ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... the samples were treated with 5% 2-mercaptoethanol in Laemmli buffer and heated at 95°C for 5 minutes prior to loading into a 4-15% gradient SDS gel (Cat# 4568085, Bio-Rad) and run at 160v for 50-55 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 150 mM NaCl, 5% Glycerol, and 500 mM imidazole. The flow-through, wash, and elution fractions were analysed on Bio-Rad 4-20% Mini-PROTEAN® TGX™ Precast Protein gels stained with Coomassie Brilliant Blue.
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bioRxiv - Biophysics 2024Quote: ... were separated on a 5-20% polyacrylamide gels (SuperSep Ace, Fujifilm Wako) and transferred onto a polyvinylidene fluoride (PVDF) or nitrocellulose membrane (Bio-Rad) using a Trans-Blot Turbo semi-dry transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... heating at 95 °C for 5 min and loading it on a 10% precast polyacrylamide gel (Bio-Rad, Hercules, California, USA). The separated bands were visualized using a ChemiDoc MP Imaging System (Bio-Rad ...
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bioRxiv - Biophysics 2024Quote: ... and eluted proteins from co-IP and surface biotinylation experiments were prepared by boiling at 95 °C for 5 minutes in SDS-PAGE sample buffer (Bio-Rad, 90% 4X Laemmli Sample Buffer + 10% 2-Mercaptoethanol) ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... Sucrose was added to achieve a final concentration of 8% and samples were loaded onto a ice-cold 5% Criterion TBE gel (Bio-Rad). Gel electrophoresis was performed in ice-cold 0.5× TBE buffer (4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... prewet in blotting buffer for 5 min) and the protein was transferred using a semi-dry system (Trans-Blot Turbo Transfer System, Bio-Rad) at 25 V 2.5 A for 20 min ...