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Citations for Bio-Rad :
5001 - 5050 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... membranes were washed again in PBS-Tween before being placed in 0.1 mL working solution (equal parts stable peroxide solution and Luminol/Enhancer solution) per cm2 membrane for 5 minutes before being placed for imaging in ChemiDoc Gel Imaging System (Bio-Rad). Gels were quantified using image J by taking the ratio of the integrated intensity (area x intensity ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fractions containing Chimera were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then treated according to the instruction of the beads and the protein complexes were then eluted by incubating at 99°C for 5 minutes with Laemmli Sample Buffer (1610747, Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... 30-50 μg of total protein was boiled at 99°C for 5 minutes with the addition of Laemmli Sample Buffer (1610747, Bio-Rad) containing 1% 2-Mercaptoethanol in a total volume of 60-80 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... blots were washed three times in TBST for 5 minutes each and incubated with an anti-mouse HRP secondary (Bio-Rad) at 1:2000 dilution for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 10 min final extension at 72°C before resolving on a 5% polyacrylamide tris-borate-EDTA (TBE) gel (Bio-Rad) at 35 V ...
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bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membranes were blocked for 1h at RT in 5% Blotting Grade Blocker Non-Fat Dry Milk (Biorad, Hercules, CA, USA) and TBST ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extractions containing 5 µg of Chl with 1 x SDS loading buffer were boiled at 100℃ for 5 min and loaded onto a 4 - 20% polyacrylamide gel (Mini Protean TGX, Biorad Laboratories). Proteins were transferred to a PVDF-FL membrane on a Biorad semidry blotting system ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad, Hercules, CA) and 5 μM each of gene-specific forward and reverse primers ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of synthesized cDNA was firstly diluted in a 1:5 ratio and used in a 20 µL reaction volume using the CFX96 Touch Real-Time PCR Detection System (BioRad, USA) with the following thermal cycling conditions ...
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bioRxiv - Biochemistry 2024Quote: ... Images were collected following 5 days of incubation at 30 °C with a Bio-Rad Chemidoc MP Imaging System (Bio-Rad) and analyzed using the ImageJ software (50).
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Cancer Biology 2024Quote: ... equipped with a 5-mL Foresight Nuvia HPQ anion-exchange column prepacked with CHT ceramic hydroxyapatite XT mixed-mode chromatography media (Bio-Rad) or 25-mL column in-house packed with the same CHT media ...
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bioRxiv - Microbiology 2023Quote: ... boiled for 5 minutes at 95°C and loaded onto Mini-Protean TGX Stain Free Gels 4-20% (Bio-Rad, P4568096). The gels were transferred onto 0.45 μm nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Pathology 2023Quote: ... Each individual and multiplexed TaqMan reaction was performed in a final volume of 10 µL containing 5 µL of Sso Advanced Universal Probes Supermix (Bio-Rad), 600 nM of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then washed five times with TBST and incubated for 5 min with ECL substrate (Clarity Western ECL substrate, Bio-Rad) in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... they were heated at 95 degrees for 5 minutes after addition of TBE Urea buffer (Novex) and immediately run on a 15% TBE-Urea gel (Bio-Rad). The gels were then imaged using Cy2 filter on ImageQuant LAS 4000 (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... in denaturing conditions after incubation into XT-sample buffer (Bio Rad) for 5 min at 90 °C with 12% Bis-Tris Criterion XT precast gels (Biorad, USA) with 1x XT-MOPS buffer at fixed current of 25 mA for 1.5-2 h ...
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bioRxiv - Biochemistry 2022Quote: ... The immunoblot was washed three times for 5 min each in TBST buffer and developed using clarity Western ECL substrate (Bio-Rad). Finally ...
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bioRxiv - Developmental Biology 2022Quote: Protein extracts were separated on a 12% SDS gel with a 5% stacking gel and transferred to PVDF membranes using the Trans-Blot Turbo System (Bio-Rad). Membranes were blocked using 5% milk for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 μg of protein extracts were run on a 4%–15% polyacrylamide gels (Mini Protein TGX Stain-Free gels, Bio-Rad) and transferred to PVDF (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... with a melt-curve from 65 to 95 °C (in 0.5 °C increments per 5 s) using CFX384 Touch Real-Time PCR Detection System (Bio-Rad, 1845384). Quantification of mRNA was determined using the comparative cycles to threshold CT (ΔΔCT ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing ADDoCoV were pooled and further purified by ion exchange chromatography (IEX) using a 5 ml Bio-Scale Mini Macro Prep High Q (Bio-Rad) equilibrated in Buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: NKX2-5 homeodomain purity was evaluated through SDS-PAGE using 15 well Mini-PROTEAN TGX Precast Protein Gels® (Bio-Rad). Samples were prepared with 4X loading buffer containing β-mercaptoethanol (BME ...
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bioRxiv - Microbiology 2022Quote: ... 20 μg of proteins of each sample or both input and eluates from the IPs were heated in Laemmli loading buffer at 95°C for 5 min and loaded on a SDS-polyacrylamide electrophoresis gel (Bio-Rad 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels ...
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bioRxiv - Cancer Biology 2023Quote: ... Quantitative PCR was performed using 4.5µl of 1:30 diluted RT product and 5.5 µl of reaction mix (composed of 5 µl of Sso Advanced Universal SYBR Green Supermix (Bio-Rad, #1725275) and 0.5 µl of forward and reverse PCR primers mixed together at a final primer concentration of 0.5 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were boiled for 5 minutes before being loaded on the gel (4-15% Criterion TGX Precast Midi Protein Gel, Bio-Rad). Proteins were separated by SDS-PAGE and blotted into a polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were washed 3 times for 5 min with TBS and were subsequently incubated with Clarity Western ECL substrate working solution (Bio-Rad) for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie Blue.
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bioRxiv - Biochemistry 2023Quote: ... The samples were incubated at 95°C for 5 min and 10 μL of the sample was resolved on a 4%–15% Mini-PROTEAN® TGX Precast Gel (Biorad) and stained with Coomassie blue dye.
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bioRxiv - Plant Biology 2023Quote: ... and 10 (V/V) % beta-mercaptoethanol for 5 minutes before being loaded into a 4-20% gradient SDS-PAGE gel (Biorad: 4561096) and run according the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg protein per sample was heated to 95 °C for 5 min in sample loading buffer and separated by SDS-PAGE using the Mini-PROTEAN electrophoresis system (Bio-Rad). Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then washed three times for 5 min each in TBST and then imaged on a ChemiDoc MP imager (Bio-Rad) using Image Lab 5.0 software using the StarBright B520 channel.
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bioRxiv - Microbiology 2023Quote: ... 1 µl of a 1:10 dilution in nuclease-free water of the cDNA samples was mixed with 5 µl of SsoAdvanced Univ SYBR Green Supermix (Bio-Rad) and 0.8 µl of 5 µM forward and reverse primers (Table S10) ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos surviving for 24 h post- injection were incubated in 100 µl of 5 % Chelex® 100 Molecular Biology Grade Resin (BioRad) and 50 µl of Proteinase K Solution (20 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad). Blotting was performed onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was washed three times with T-TBS and twice with TBS for 5 min each and incubated with Clarity Western ECL substrate (Bio-Rad). After 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µl of input and 5 µl of IP samples were loaded and protein samples were separated on 4-20% Tris-glycine polyacrylamide gels (Bio-Rad) at 170 V for 1 hour in 1X Tris/glycine/SDS running buffer ...
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bioRxiv - Microbiology 2023Quote: ... Samples (24 µg) were denatured by heating 5 minutes at 95°C and separated by Stain-Free SDS-PAGE gel (4568096, Bio-Rad). The gel was activated using one-minute setting (Bio-Rad Imager ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then boiled at 95°C for 5 minutes and 20 μL of the samples were loaded into a pre-made polyacrylamide gel (Bio-Rad) immersed in 1x Tris-Glycine-SDS Buffer ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5×106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Microbiology 2023Quote: A volume of 5 μL of cDNA was used for quantitative PCR (qPCR) on a CFX Connect Real- Time PCR Detection System (Bio-Rad). TaqMan and SYBR Green qPCRs were performed as described previously (Benschop et al. ...