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Citations for Bio-Rad :
4901 - 4950 of 5277 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... One or two micrograms of the concentrated viruses were heated at 95 °C for 5 min before being resolved on 4-20% SDS-PAGE (Bio-Rad) using the Novex™ Sharp Pre-stained Protein Standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Real time quantitative PCR (qRT-PCR) was performed using an iQ™5 Multicolor Real-Time PCR Detection System (BIO-RAD) with qPCRBIO SyGreen Mix fluorescein (PCR BIOSYSTEMS) ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in nuclease-free water and the primer(s) were incubated at 65°C for 5 minutes in the DNA Engine Dyad®Peltier Thermal Cycler (BioRad). Afterward ...
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bioRxiv - Cell Biology 2020Quote: ... to pH 8.88 first for 5 min at 95 °C followed by 60 °C for 3 h on a T100 Thermal Cycler (Bio-Rad) with the heated lid set to 105 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The blots were washed four times with TBST for 5 minutes each before developing with Clarity Western ECL Blotting Substrates (Bio-Rad) and imaging ...
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bioRxiv - Immunology 2021Quote: ... They were incubated at the room temperature for 5-10 min and they were placed inside a 4-mm cuvette (Bio-Rad). The cuvettes were pulsed using the BTX electroporator (300 V ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were washed three times with TBST for 5 minutes and incubated with Goat Anti-Rabbit IgG (Bio-Rad, 170-6515) for 1 hour at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to 10 μL RNA and incubating at 65°C for 5 min on a C1000 Touch Thermal Cycler (Bio-Rad, USA) followed by chilling on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 seconds at 72°C and a final extension of 5 minutes at 72 °C in a thermal cycler (T100TM Thermal Cycler, Bio-Rad). In order to standardize the expression pattern of NvDsx ...
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bioRxiv - Biochemistry 2022Quote: ... and heated to 100 °C for 5 min and resolved on 4-20% (w/v) gradient SDS-PAGE gels (Bio-Rad) with Tris-glycine buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was mixed with 5× RT Reaction Buffer and 10× RT Enzyme Mix and transferred to a thermal cycler (Bio-Rad). Cycling conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were cleared by centrifugation at 16000xg for 5 min at 4°C and the total protein concentration in the lysates was measured by Bradford protein assay (Bio-Rad). For western blot analysis 25 µg of each lysate was boiled for 5 min at 98°C (for the detection of LC3B and ATG9A samples were heated at 60°C for 10 min ...
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bioRxiv - Developmental Biology 2020Quote: ... excess antibody subsequently removed by a further 4 washes in TBSTw for 5 minutes before detection with ECL Western blotting reagent (Bio-Rad) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
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bioRxiv - Bioengineering 2021Quote: ... This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad) for 1 h with rocking ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.5 to a salt concentration of 100 mM NaCl and loaded onto a 5 mL Bio-Scale TM Mini CHT Type II column (Bio-Rad) equilibrated with CHT column buffer (5% glycerol (w/v) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Protein was eluted from beads by heating for 5 min at 95 °C in 1X Laemmli Buffer (Bio-Rad, Hercules, CA). Retrieved protein was detected by standard immunoblotting techniques using the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg of neuronal lysate and corresponding mHTTex1 seed were boiled for 5 min with sample loading buffer and loaded into a 4-20 % polyacrylamide gel (SDS-PAGE, Criterion Bio-Rad) to detect monomeric endogenous HTT or by semi-denaturing detergent agarose (1.5% ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 min and proteins were resolved on 10% SDS polyacrylamide gels and blotted onto nitrocellulose membranes (Bio-Rad). Anti-rabbit Phospho-p44/42 MAPK antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... Assays comprising of premixtures of a forward and reverse primer (18 μM each) with a FAM- or HEX-conjugated hydrolysis probe (5 μM) were either purchased from Bio-Rad, based on previous publications (Roberts et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Gene expression analysis were performed by quantitative RT-qPCR in a Viia7PCR device with 5 μl reaction mix containing SyberGreen Supermix (Bio-Rad). Tomato actin was used as a reference gene and fold change in expression were calculated based on 2^(-ddCt ...
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bioRxiv - Bioengineering 2021Quote: ... 15 µg of the extracted protein samples were boiled for 5 minutes at 95 °C in 4X laemlli buffer (BioRad; 1610747), run on mini-PROTEAN TGX precast gels (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Plates were incubated for 30 °C for 5 or 7 days and imaged using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... and boiled for 5 min prior to loading on 4-20% SDS-PAGE gradient gels (Mini-PROTEAN TGX precast gels; Bio-Rad). Precision Plus Protein Unstained Protein Standards (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... The total PCR reaction volume was 15 µL per well (10 µL master mix + 5 µL cDNA sample) and the qPCR was performed on the CFX384 Touch Real-Time PCR Detection System (Bio-Rad) using the cycling conditions listed below:
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bioRxiv - Physiology 2021Quote: ... Protein aliquots were denatured in Laemmli sample buffer at 100°C for 5 min and then loaded (10– 15 μg protein per lane) on 4–20% Criterion TGX Stain-free gels (Bio-Rad) and run for 45 min at 200 V ...
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bioRxiv - Microbiology 2020Quote: ... followed by ExtrAvidin alkaline phosphatase conjugate and alkaline phosphatase substrate: p-nitro blue tetrazolium chloride enhanced 5-bromo-4chloro-3-indolyl phosphate (Bio-Rad). The membranes with the transferred rgp120 were incubated with the HRP-conjugated V5 antibody (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed again for 3 × 5-min in TBS-T before being immersed for 5 min under gentle agitation at room temperature in Clarity ECL detection substrate (Bio-rad Laboratories Pty Ltd ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were boiled for 5 min and the proteins were separated in 4-20% Mini-PROTEAN TGX Precast Protein Gel (Bio-RAD) and electroblotted to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2022Quote: ... Standard qPCR reactions with 5 or 10 ng cDNA per reaction were performed with iTaq Universal SYBR Green Supermix (Bio-Rad) in the CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were run at 90 V for 120 min and there-after stained with Ethidium bromide at room temperature for 5 min and visualised using a Gel Doc XR imaging system (Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... cell density was between 4-5 × 106 cells/mL with >95% viability as determined using a TC20 automated cell counter (Bio-Rad). Cells were diluted with warm Expi293 Expression Media to 2 × 106 cells/mL in 42.5 mL ...
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bioRxiv - Molecular Biology 2022Quote: ... for 30 minutes at room temperature was followed by three 5 minute washes in milliQ water before imaging on a ChemiDoc MP (Bio-Rad).
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
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bioRxiv - Cell Biology 2022Quote: ... denatured in 2x Laemmli buffer (5 min at +95°C) and 20 µg were loaded per well of Mini-PROTEAN Precast Gel (Bio-Rad). The protein transfer was done with the Trans-Blot Turbo system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were incubated at 95 °C for 5 minutes prior to being resolved on a 4 – 15% Mini-PROTEAN TGX Precast Protein Gels (Bio-rad) and transferred onto a methanol activated Immobilon-P PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... The stoichiometry of condensin II subunits for each preparation was checked by 5% SDS-PAGE followed by Coomassie Brilliant Blue R-250 (Bio-Rad) staining ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with beta-mercaptoethanol and denaturated at 95 °C for 5 min before loading on a 12 % PAA precast gel (#4561044, Bio-Rad). The gel was run at 70 V for 15 min and then 100 V for 1 h ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein lysates were prepared from 25 pair of testes and loaded in equal dilution series (5, 10, and 20 μg) onto precast stain-free PAAG gel (Bio-Rad). After electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25% of beads by heating at 95°C for 5 minutes in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from the remaining 75% of beads using TRIzol (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were boiled for 5 min at 95°C in 1x XT sample buffer containing 1x XT reducing agent (both from Bio-Rad) and resolved on 4-12% Bis-Tris XT precast gels (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: Protein samples were denatured for 5 min at 95 °C and run on Mini or Midi TGX-Stain-free precast gels (Bio-rad) at 110V for 60 min (mini gels ...
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bioRxiv - Immunology 2020Quote: ... polβ and Exo1 to the 5’Sμ (in CH12-F3) was assessed by quantifying ChIP-enriched DNA using CFX384 realtime PCR (Bio-Rad). The following primers were used for qPCR:
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed four times in PBST for 5 minutes at room temperature and imaged directly using a ChemiDoc MP imaging system (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... 14 µL of PCR product were then transformed by electroporation in 90 µL cells (2.5 kV electric pulse for 5 ms, Bio-Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...
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bioRxiv - Biochemistry 2022Quote: ... heated at 95 °C for 5 min and resolved on a 4–12% Criterion XT Bis-Tris Protein Gel (Cat. No. 3450125, Bio-Rad). Proteins on the gel were transferred to the PVDF membrane ...
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bioRxiv - Immunology 2022Quote: ... Transfection took place over two rounds of electroporation at 850 V and 25 μF with an interval of 5 secs (Bio-Rad Gene Pulser Xcell Electroporation Systems) ...